MD11G1116500.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012947
Physical Location & Seq
Forward (+)
10526802 .. 10528163
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1116500.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGTGGAAGCTTTTTGATAAGAGGAGAGACCAAGAGTGCTACATTTTGGACTACCATTGCTACAAGCCCACAGATGACAGAAAGCTAGACACTGAGTTCTCAGGTGAGATTATCAAGAGGACCAAGAATCTTGGTCTTTTGGAATACCAGTTCCTCCTCAAGGCAATTGTAAGCTCAGGGATTGGTGAGCAAACTTATGCTCCAAGAATAATGTTTTCAGGCAGGGAATCATGTCCCACATTGGACGACAGCATCTCTGAGATGGAGGAGTTCTTCCATGATAGCATCAAGAAACTCCTTGACAGATCAGGGATTTCACCTTCAGAAATTGATGTGCTTGTTGTGAATGTCTCCATGTTTGCATCTGTTCCATCTTTGTCCTCCAGGATTATAAACCACTACAAGATGAGGGAGAACATAAAGGTCTTTAACCTCAGTGGGATGGGCTGCTCAGCATCCCTAATCTCAGTTGACATAGTGAAAAACATTTTCAAGTCACACAGAAATGTTTATGCACTTGTGGTCACTTCTGAGTCCTTGAGCCCCAATTGGTATTCAGGCAATGCCAGATCCATGATTCTGGCAAACTGTTTGTTCAGGTCCGGCGGTTGCGCGATCCTTCTGACGAACAAACGGGCCTTAAAACACCGAGCCATGTTCAAATTGAAATGCTTGGTGAGAACCCATCACGGCGGGAGAGACGATTCCTACGGGTGCTGCATCCAACAAGAAGATACACAAGGGTATCTAGGGTTTCACCTAGACAAATACTTACCCAAGGCGGCCACACGAGCTTTTGTGGACAACCTAAGGGTAATATCCCCCAAGATTTTACCCATCAGGGAGCTAATGAGGTTTTTGCTTGTAACCCTGGTGAAAAAAAAGACCTCCTCACAACAATCCACCAAGGGAGGGGCTATTTCTAGCCCATCACCAAAACCTGTGATCAATTTCAAAACTGGGGTGGATCATTTTTGCATCCACACCGGTGGGAAGGCGGTGATCGACGGCATAGGATTAAATCTTGGTCTCAGCGAGTATGATCTGGAACCTGCAAGGATGACACTCCACAGATTTGGGAACACATCAGCAAGCAGCCTCTGGTATGTGTTGGGATACATGGAGGCAAAGAAGAGGTTGAAGAAAGGGGATACTGTTTTGATGATAAGCTTTGGAGCCGGGTTTAAATGCAACAGTTGCTTGTTGGAGGTGGTAAGGGATTTGGGGGATGAAAATGTTTGGAAGGAGGACATTGCTACGTACCCACCAAAGACGCTCACAAACCCTTTCTTGGAGAAGTTTGGTTGGATTCGTCATGAGGATCCAAGCACATTCAACGTGGATAATTATTTGAACCTTTAA

Protein Analysis

454

Amino Acids

51.14

Weight (kDa)

9.02

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 7 - 295 1.3e-118 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 322 - 403 1.7e-13 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 325 - 404 8.8e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07720 AT1G07720 AT2G28630 AT2G28630
fragaria_vesca FvH4_3g34690
malus_domestica MD03G1102500.v1.1 MD11G1116500.v1.1
prunus_persica Prupe.6G086800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0062381
rosa_laevigata RLG00000035554
rosa_multiflora Rmu_sc0005798.1_g000006
rosa_roxburghii Rroxscaffold_1G00018280
rosa_rugosa Rorug05G0349200
rosa_samantha Rh5AG408700 Rh5BG423100 Rh5CG447000 Rh5DG437800
rosa_wichuraiana Rw5G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
Acc36I ACCTGC 1 cut(s) 1060
AccII CGCG 1 cut(s) 614
AciI CCGC 4 cut(s) 606, 693, 782, 998
AclWI GGATC 5 cut(s) 564, 610, 975, 1316, 1329
AcoI YGGCCR 1 cut(s) 783
AcuI CTGAAG 1 cut(s) 306
AfaI GTAC 1 cut(s) 1262
AfiI CCNNNNNNNGG 3 cut(s) 160, 912, 1291
AgeI ACCGGT 1 cut(s) 986
AgsI TTSAA 7 cut(s) 493, 661, 667, 955, 1141, 1336, 1354
AjnI CCWGG 2 cut(s) 383, 870
AleI CACNNNNGTG 1 cut(s) 987
AloI GAACNNNNNNTCC 2 cut(s) 134, 166
AluBI AGCT 6 cut(s) 10, 85, 174, 794, 847, 1170
AluI AGCT 6 cut(s) 10, 85, 174, 794, 847, 1170
Alw26I GTCTC 4 cut(s) 21, 355, 693, 1034
AlwI GGATC 5 cut(s) 564, 610, 975, 1316, 1329
AlwNI CAGNNNCTG 1 cut(s) 1101
AoxI GGCC 2 cut(s) 636, 783
ApeKI GCWGC 3 cut(s) 447, 717, 1095
AsiGI ACCGGT 1 cut(s) 986
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 3 cut(s) 120, 600, 636
AsuC2I CCSGG 1 cut(s) 1180
AsuHPI GGTGA 8 cut(s) 116, 197, 309, 688, 749, 886, 924, 1012
AvaII GGWCC 2 cut(s) 120, 600
AxyI CCTNAGG 1 cut(s) 809
BaeI ACNNNNGTAYC 2 cut(s) 728, 761
BamHI GGATCC 1 cut(s) 1321
BanII GRGCYC 1 cut(s) 545
BauI CACGAG 1 cut(s) 789
BbvI GCAGC 3 cut(s) 434, 704, 1107
BccI CCATC 6 cut(s) 256, 379, 436, 693, 845, 937
BceAI ACGGC 2 cut(s) 706, 1024
BciT130I CCWGG 2 cut(s) 385, 872
BciVI GTATCC 2 cut(s) 1109, 1144
BclI TGATCA 1 cut(s) 945
BcnI CCSGG 1 cut(s) 1180
BcoDI GTCTC 4 cut(s) 21, 355, 693, 1034
BfaI CTAG 4 cut(s) 86, 749, 761, 924
BfuAI ACCTGC 1 cut(s) 1060
BfuI GTATCC 2 cut(s) 1109, 1144
BisI GCNGC 4 cut(s) 448, 718, 783, 1096
BlpI GCTNAGC 1 cut(s) 451
BlsI GCNGC 4 cut(s) 449, 719, 784, 1097
Bme1390I CCNGG 3 cut(s) 385, 872, 1180
Bme18I GGWCC 2 cut(s) 120, 600
BmgT120I GGNCC 3 cut(s) 120, 600, 636
BmiI GGNNCC 3 cut(s) 1050, 1177, 1323
BmrFI CCNGG 3 cut(s) 385, 872, 1180
BmrI ACTGGG 1 cut(s) 969
BmsI GCATC 6 cut(s) 261, 294, 371, 464, 729, 987
BmuI ACTGGG 1 cut(s) 969
BpmI CTGGAG 1 cut(s) 367
Bpu10I CCTNAGC 1 cut(s) 175
Bpu1102I GCTNAGC 1 cut(s) 451
BpuEI CTTGAG 2 cut(s) 143, 559
BpuMI CCSGG 1 cut(s) 1180
BsaAI YACGTR 1 cut(s) 1260
BsaI GGTCTC 2 cut(s) 21, 1034
BsaJI CCNNGG 3 cut(s) 777, 870, 906
BsaWI WCCGGW 1 cut(s) 986
BsaXI ACNNNNNCTCC 2 cut(s) 184, 214
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 912, 1291
Bse118I RCCGGY 1 cut(s) 986
Bse1I ACTGG 2 cut(s) 148, 964
Bse21I CCTNAGG 1 cut(s) 809
Bse3DI GCAATG 3 cut(s) 55, 568, 1251
BseBI CCWGG 2 cut(s) 385, 872
BseDI CCNNGG 3 cut(s) 777, 870, 906
BseGI GGATG 6 cut(s) 447, 455, 720, 978, 1065, 1234
BseLI CCNNNNNNNGG 3 cut(s) 160, 912, 1291
BseMI GCAATG 3 cut(s) 55, 568, 1251
BseMII CTCAG 9 cut(s) 84, 114, 189, 249, 448, 465, 480, 522, 1045
BseNI ACTGG 2 cut(s) 148, 964
BseRI GAGGAG 4 cut(s) 37, 146, 281, 880
BseXI GCAGC 3 cut(s) 434, 704, 1107
Bsh1236I CGCG 1 cut(s) 614
BshFI GGCC 2 cut(s) 638, 785
BshTI ACCGGT 1 cut(s) 986
BsiSI CCGG 3 cut(s) 603, 987, 1179
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 3 cut(s) 160, 912, 1291
BsmAI GTCTC 4 cut(s) 21, 355, 693, 1034
BsmBI CGTCTC 1 cut(s) 693
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 2 cut(s) 638, 785
Bso31I GGTCTC 2 cut(s) 21, 1034
Bsp1286I GDGCHC 1 cut(s) 545
Bsp143I GATC 8 cut(s) 305, 569, 615, 945, 967, 1002, 1042, 1321
Bsp1720I GCTNAGC 1 cut(s) 451
BspACI CCGC 4 cut(s) 606, 693, 782, 998
BspANI GGCC 2 cut(s) 638, 785
BspCNI CTCAG 9 cut(s) 85, 113, 188, 250, 447, 464, 479, 523, 1044
BspFNI CGCG 1 cut(s) 614
BspHI TCATGA 1 cut(s) 1315
BspLI GGNNCC 3 cut(s) 1050, 1177, 1323
BspMI ACCTGC 1 cut(s) 1060
BspPI GGATC 5 cut(s) 564, 610, 975, 1316, 1329
BspTNI GGTCTC 2 cut(s) 21, 1034
BsrDI GCAATG 3 cut(s) 55, 568, 1251
BsrFI RCCGGY 1 cut(s) 986
BsrI ACTGG 2 cut(s) 148, 964
BssAI RCCGGY 1 cut(s) 986
BssECI CCNNGG 3 cut(s) 777, 870, 906
BssMI GATC 8 cut(s) 305, 569, 615, 945, 967, 1002, 1042, 1321
BssSI CACGAG 1 cut(s) 789
BssT1I CCWWGG 2 cut(s) 777, 906
Bst2BI CACGAG 1 cut(s) 789
Bst2UI CCWGG 2 cut(s) 385, 872
Bst4CI ACNGT 3 cut(s) 590, 1156, 1196
Bst6I CTCTTC 1 cut(s) 1127
BstAPI GCANNNNNTGC 1 cut(s) 1197
BstBAI YACGTR 1 cut(s) 1260
BstC8I GCNNGC 1 cut(s) 1093
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 6 cut(s) 447, 455, 720, 978, 1065, 1234
BstFNI CGCG 1 cut(s) 614
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 8 cut(s) 308, 572, 618, 948, 970, 1005, 1045, 1324
BstMAI GTCTC 4 cut(s) 21, 355, 693, 1034
BstMBI GATC 8 cut(s) 305, 569, 615, 945, 967, 1002, 1042, 1321
BstMWI GCNNNNNNNGC 2 cut(s) 791, 1197
BstNI CCWGG 2 cut(s) 385, 872
BstSCI CCNGG 3 cut(s) 383, 870, 1178
BstSNI TACGTA 1 cut(s) 1260
BstUI CGCG 1 cut(s) 614
BstV1I GCAGC 3 cut(s) 434, 704, 1107
BstX2I RGATCY 2 cut(s) 569, 1321
BstXI CCANNNNNNTGG 3 cut(s) 580, 989, 1076
BstYI RGATCY 2 cut(s) 569, 1321
Bsu36I CCTNAGG 1 cut(s) 809
BsuI GTATCC 2 cut(s) 1109, 1144
BsuRI GGCC 2 cut(s) 638, 785
BtsCI GGATG 6 cut(s) 447, 455, 720, 978, 1065, 1234
BtsIMutI CAGTG 2 cut(s) 90, 442
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 1 cut(s) 1093
CaiI CAGNNNCTG 1 cut(s) 1101
CciI TCATGA 1 cut(s) 1315
CfoI GCGC 1 cut(s) 614
Cfr10I RCCGGY 1 cut(s) 986
Cfr13I GGNCC 3 cut(s) 120, 600, 636
CseI GACGC 1 cut(s) 1282
Csp6I GTAC 1 cut(s) 1261
CspAI ACCGGT 1 cut(s) 986
CviAII CATG 7 cut(s) 231, 278, 355, 574, 655, 1120, 1316
CviQI GTAC 1 cut(s) 1261
DpnI GATC 8 cut(s) 307, 571, 617, 947, 969, 1004, 1044, 1323
DpnII GATC 8 cut(s) 305, 569, 615, 945, 967, 1002, 1042, 1321
DraI TTTAAA 1 cut(s) 1186
EaeI YGGCCR 1 cut(s) 783
Eam1104I CTCTTC 1 cut(s) 1127
EarI CTCTTC 1 cut(s) 1127
Eco105I TACGTA 1 cut(s) 1260
Eco130I CCWWGG 2 cut(s) 777, 906
Eco24I GRGCYC 1 cut(s) 545
Eco31I GGTCTC 2 cut(s) 21, 1034
Eco47I GGWCC 2 cut(s) 120, 600
Eco57I CTGAAG 1 cut(s) 306
Eco81I CCTNAGG 1 cut(s) 809
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 383, 870
EcoT14I CCWWGG 2 cut(s) 777, 906
EcoT38I GRGCYC 1 cut(s) 545
ErhI CCWWGG 2 cut(s) 777, 906
Esp3I CGTCTC 1 cut(s) 693
FaeI CATG 7 cut(s) 234, 281, 358, 577, 658, 1123, 1319
FaqI GGGAC 1 cut(s) 219
FatI CATG 7 cut(s) 230, 277, 354, 573, 654, 1119, 1315
FauI CCCGC 1 cut(s) 686
FbaI TGATCA 1 cut(s) 945
Fnu4HI GCNGC 4 cut(s) 448, 718, 783, 1096
FokI GGATG 6 cut(s) 442, 454, 707, 965, 1072, 1241
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 4 cut(s) 448, 718, 783, 1096
FspBI CTAG 4 cut(s) 86, 749, 761, 924
GlaI GCGC 1 cut(s) 613
GluI GCNGC 4 cut(s) 448, 718, 783, 1096
GsuI CTGGAG 1 cut(s) 367
HaeIII GGCC 2 cut(s) 638, 785
HapII CCGG 3 cut(s) 603, 987, 1179
HgaI GACGC 1 cut(s) 1282
HhaI GCGC 1 cut(s) 614
Hin1II CATG 7 cut(s) 234, 281, 358, 577, 658, 1123, 1319
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 2 cut(s) 8, 1168
HinfI GANTC 6 cut(s) 127, 227, 533, 577, 704, 1309
HpaII CCGG 3 cut(s) 603, 987, 1179
HphI GGTGA 8 cut(s) 116, 197, 309, 688, 749, 886, 924, 1012
Hpy166II GTNNAC 2 cut(s) 472, 802
Hpy188I TCNGA 4 cut(s) 259, 325, 532, 624
Hpy188III TCNNGA 4 cut(s) 115, 289, 1046, 1316
Hpy8I GTNNAC 2 cut(s) 472, 802
Hpy99I CGWCG 1 cut(s) 1010
HpyAV CCTTC 4 cut(s) 330, 629, 988, 1237
HpyCH4III ACNGT 3 cut(s) 590, 1156, 1196
HpyCH4IV ACGT 2 cut(s) 1259, 1338
HpyCH4V TGCA 6 cut(s) 362, 515, 720, 978, 1055, 1191
HpyF10VI GCNNNNNNNGC 2 cut(s) 791, 1197
HpySE526I ACGT 2 cut(s) 1259, 1338
Hsp92II CATG 7 cut(s) 234, 281, 358, 577, 658, 1123, 1319
HspAI GCGC 1 cut(s) 612
Ksp22I TGATCA 1 cut(s) 945
Kzo9I GATC 8 cut(s) 305, 569, 615, 945, 967, 1002, 1042, 1321
LmnI GCTCC 3 cut(s) 205, 844, 1175
Lsp1109I GCAGC 3 cut(s) 434, 704, 1107
LweI GCATC 6 cut(s) 261, 294, 371, 464, 729, 987
MaeI CTAG 4 cut(s) 86, 749, 761, 924
MaeII ACGT 2 cut(s) 1259, 1338
MaeIII GTNAC 3 cut(s) 495, 523, 865
MalI GATC 8 cut(s) 307, 571, 617, 947, 969, 1004, 1044, 1323
MboI GATC 8 cut(s) 305, 569, 615, 945, 967, 1002, 1042, 1321
MboII GAAGA 4 cut(s) 265, 743, 1144, 1153
MfeI CAATTG 2 cut(s) 165, 547
MflI RGATCY 2 cut(s) 569, 1321
MhlI GDGCHC 1 cut(s) 545
MluCI AATT 6 cut(s) 165, 327, 547, 662, 949, 1345
MlyI GAGTC 1 cut(s) 542
MmeI TCCRAC 3 cut(s) 748, 1185, 1286
MseI TTAA 5 cut(s) 429, 641, 1019, 1185, 1360
MslI CAYNNNNRTG 2 cut(s) 504, 987
MspI CCGG 3 cut(s) 603, 987, 1179
MspR9I CCNGG 3 cut(s) 385, 872, 1180
MunI CAATTG 2 cut(s) 165, 547
MvaI CCWGG 2 cut(s) 385, 872
MvnI CGCG 1 cut(s) 614
MwoI GCNNNNNNNGC 2 cut(s) 791, 1197
NciI CCSGG 1 cut(s) 1180
NdeII GATC 8 cut(s) 305, 569, 615, 945, 967, 1002, 1042, 1321
NlaIII CATG 7 cut(s) 234, 281, 358, 577, 658, 1123, 1319
NlaIV GGNNCC 3 cut(s) 1050, 1177, 1323
NmuCI GTSAC 2 cut(s) 495, 523
OliI CACNNNNGTG 1 cut(s) 987
PagI TCATGA 1 cut(s) 1315
PfeI GAWTC 5 cut(s) 127, 227, 577, 704, 1309
PfoI TCCNGGA 1 cut(s) 383
PinAI ACCGGT 1 cut(s) 986
PkrI GCNGC 4 cut(s) 449, 719, 784, 1097
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
Ppu21I YACGTR 1 cut(s) 1260
PsiI TTATAA 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 383, 870
PspGI CCWGG 2 cut(s) 383, 870
PspN4I GGNNCC 3 cut(s) 1050, 1177, 1323
PspPI GGNCC 3 cut(s) 120, 600, 636
PstNI CAGNNNCTG 1 cut(s) 1101
PsuI RGATCY 2 cut(s) 569, 1321
RsaI GTAC 1 cut(s) 1262
RsaNI GTAC 1 cut(s) 1261
RseI CAYNNNNRTG 2 cut(s) 504, 987
SaqAI TTAA 5 cut(s) 429, 641, 1019, 1185, 1360
SatI GCNGC 4 cut(s) 448, 718, 783, 1096
Sau3AI GATC 8 cut(s) 305, 569, 615, 945, 967, 1002, 1042, 1321
Sau96I GGNCC 3 cut(s) 120, 600, 636
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 3 cut(s) 385, 872, 1180
SduI GDGCHC 1 cut(s) 545
SfaNI GCATC 6 cut(s) 261, 294, 371, 464, 729, 987
SgrAI CRCCGGYG 1 cut(s) 986
SinI GGWCC 2 cut(s) 120, 600
SmiMI CAYNNNNRTG 2 cut(s) 504, 987
SmlI CTYRAG 2 cut(s) 158, 538
SmoI CTYRAG 2 cut(s) 158, 538
SnaBI TACGTA 1 cut(s) 1260
Sse9I AATT 6 cut(s) 165, 327, 547, 662, 949, 1345
SsiI CCGC 4 cut(s) 606, 693, 782, 998
SspMI CTAG 4 cut(s) 86, 749, 761, 924
StyD4I CCNGG 3 cut(s) 383, 870, 1178
StyI CCWWGG 2 cut(s) 777, 906
TaaI ACNGT 3 cut(s) 590, 1156, 1196
TaiI ACGT 2 cut(s) 1262, 1341
TaqI TCGA 1 cut(s) 1005
TasI AATT 6 cut(s) 165, 327, 547, 662, 949, 1345
TauI GCSGC 1 cut(s) 785
TfiI GAWTC 5 cut(s) 127, 227, 577, 704, 1309
Tru1I TTAA 5 cut(s) 429, 641, 1019, 1185, 1360
Tru9I TTAA 5 cut(s) 429, 641, 1019, 1185, 1360
TscAI CASTG 2 cut(s) 97, 442
TseFI GTSAC 2 cut(s) 495, 523
TseI GCWGC 3 cut(s) 447, 717, 1095
Tsp45I GTSAC 2 cut(s) 495, 523
TspDTI ATGAA 1 cut(s) 1245
TspRI CASTG 2 cut(s) 97, 442
VpaK11BI GGWCC 2 cut(s) 120, 600
XagI CCTNNNNNAGG 1 cut(s) 158
XspI CTAG 4 cut(s) 86, 749, 761, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.