Rw5G038550

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012947
Physical Location & Seq
Reverse (-)
67902708 .. 67904377
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G038550.1

Sequence Viewer

Length: 1407 bp
ATGGAGTTTCTGAGTTTACTCTATGCTTTCTCAGCTTTGTATTTCATGTTCACTTTGTGGAAGCGTTATGGTGAGAAAAGAGACCAACAATGTTACATCTTGGACTACCAATGCCACAAGCCCACAGATGACCGAAAGCTTGACACAGAGTATTCAGGGGAGATCATCAAGAGGACCAAGAATTTGGGTCTTCTAGAGTACCAGTTCCTCCTCAAAGCCATAGTGAGCTCCGGCATTGGCGAGCAAACCTACGCGCCGAGGATCATGTTTGACGGCAGGGAATCATGTCCCACATTGGAAGATTCAATCACAGAGATGGAGGAGTTCTTCCATGACAGCATTCAGAAGGTGTTGGACAGACAAGGCCTTTCCCCTTCACAAATTGATGTGCTTGTGGTCAATGTGTCAATGTTTGCTTCTGTGCCTTCTTTAGCCTCAAGGATTATAAACCACTACAAGATGAGAGAGGACATAAAGGTCTATAACCTCAGCGGAATGGGTTGCTCAGCTAGTCTAATCTCAGTTGGCATAGTCAGGAGCATTTTCAAGTCAAACAAGAATATTTATGCACTTGTGGTCACTTCAGAGTCTCTGAGCCCCAATTGGTACACAGGTAATGCCAGATCCATGATTCTTGCAAATTGTTTGTTTAGGTCTGGTGGCTGTGCTATCATTTTGACTAACAAAAGGGCCTTGAAAAACCAAGCCATGTTCAAACTGAACTGTTTGGTTAGAACCCATCATGGGGCCAAAGATGAGTCCTATGGTTGTTGCATACAACAAGAAGATGAGCAAGGGAGGCTTGGGTTTCACCTCGCCAAGATTTTGCCCAAGGCTGCCACAAGAGCTTTTGTGGACAACCTTAGGGAAATTTCTCCCAAAATCTTGCCCATCAGGGAGTTGGTTAGGTTTTTGCTTGCAACCCTTTTGAGGAAATTGAGAGAAAAGAGCAGCTCATCCTCTAAGGGATTAGGGACTTCTCCCCCAAAACCTGTGATAGACTTCAAAACTGGGGTTGATCACTTCTGCATTCACACCGGCGGAAAGGCAGTGATAGATGGAATTGGCAAGAGTCTTGGCCTCACTGAACATGACCTTGAGCCTGCAAGGATGACACTCCACAGGTTTGGGAATACTTCTGCAAGTAGTCTCTGGTATGTCTTGGCTTACATGGAGGCCAAGAAGAGGCTCAAGAAGGGTGATACAGTGTTGATGATAAGCTTTGGTGCTGGCTTTAAATGCAACAGCTGTTTTCTGGAGGTGGCGAGGGATTTGGAGGATGGCAATGTCTGGAAAGAGGAAATTGCTATGTACCCACCAAAGTCCTTGACCAACCCTTTCATGGAGAAATTTGGTTGGCTCCAACAAGAGGACCCAAGCACATTCAAGCTTGAAGATTTTCTATAA

Protein Analysis

468

Amino Acids

52.91

Weight (kDa)

8.75

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 25 - 312 1.6e-115 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 339 - 420 5.4e-15 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 342 - 417 5.2e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07720 AT1G07720 AT2G28630 AT2G28630
fragaria_vesca FvH4_3g34690
malus_domestica MD03G1102500.v1.1 MD11G1116500.v1.1
prunus_persica Prupe.6G086800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0062381
rosa_laevigata RLG00000035554
rosa_multiflora Rmu_sc0005798.1_g000006
rosa_roxburghii Rroxscaffold_1G00018280
rosa_rugosa Rorug05G0349200
rosa_samantha Rh5AG408700 Rh5BG423100 Rh5CG447000 Rh5DG437800
rosa_wichuraiana Rw5G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
AasI GACNNNNNNGTC 1 cut(s) 476
AccII CGCG 1 cut(s) 254
AciI CCGC 2 cut(s) 492, 1041
AclWI GGATC 2 cut(s) 269, 618
AcsI RAATTY 3 cut(s) 181, 870, 1349
AcuI CTGAAG 1 cut(s) 567
AdeI CACNNNGTG 1 cut(s) 57
AfaI GTAC 3 cut(s) 200, 608, 1313
AfiI CCNNNNNNNGG 6 cut(s) 744, 745, 930, 1185, 1342, 1369
AgsI TTSAA 7 cut(s) 306, 547, 697, 715, 1006, 1387, 1394
AluBI AGCT 9 cut(s) 35, 139, 228, 509, 848, 954, 1221, 1248, 1390
AluI AGCT 9 cut(s) 35, 139, 228, 509, 848, 954, 1221, 1248, 1390
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 3 cut(s) 75, 594, 1154
AlwI GGATC 2 cut(s) 269, 618
AoxI GGCC 5 cut(s) 364, 690, 747, 1078, 1176
ApeKI GCWGC 2 cut(s) 836, 951
ApoI RAATTY 3 cut(s) 181, 870, 1349
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
Asp700I GAANNNNTTC 1 cut(s) 1398
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 4 cut(s) 174, 690, 747, 1372
AsuHPI GGTGA 3 cut(s) 83, 803, 1211
AvaII GGWCC 2 cut(s) 174, 1372
AxyI CCTNAGG 1 cut(s) 863
BaeI ACNNNNGTAYC 2 cut(s) 598, 631
BanII GRGCYC 2 cut(s) 230, 599
BbsI GAAGAC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 230
BbvCI CCTCAGC 1 cut(s) 488
BbvI GCAGC 2 cut(s) 823, 963
BccI CCATC 5 cut(s) 310, 747, 899, 1052, 1274
BceAI ACGGC 1 cut(s) 289
BclI TGATCA 1 cut(s) 1018
BcoDI GTCTC 3 cut(s) 75, 594, 1154
BfaI CTAG 2 cut(s) 194, 510
BisI GCNGC 2 cut(s) 837, 952
BlpI GCTNAGC 1 cut(s) 505
BlsI GCNGC 2 cut(s) 838, 953
Bme18I GGWCC 2 cut(s) 174, 1372
BmgT120I GGNCC 4 cut(s) 174, 690, 747, 1372
BmiI GGNNCC 3 cut(s) 748, 1361, 1374
BmrI ACTGGG 1 cut(s) 1020
BmuI ACTGGG 1 cut(s) 1020
BpiI GAAGAC 1 cut(s) 182
BpmI CTGGAG 1 cut(s) 1277
Bpu10I CCTNAGC 1 cut(s) 488
Bpu1102I GCTNAGC 1 cut(s) 505
BpuEI CTTGAG 3 cut(s) 421, 1118, 1175
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 257, 831
Bsc4I CCNNNNNNNGG 6 cut(s) 744, 745, 930, 1185, 1342, 1369
Bse118I RCCGGY 1 cut(s) 1037
Bse1I ACTGG 2 cut(s) 202, 1015
Bse21I CCTNAGG 1 cut(s) 863
Bse3DI GCAATG 1 cut(s) 1291
BseDI CCNNGG 2 cut(s) 257, 831
BseGI GGATG 3 cut(s) 956, 1116, 1285
BseLI CCNNNNNNNGG 6 cut(s) 744, 745, 930, 1185, 1342, 1369
BseMI GCAATG 1 cut(s) 1291
BseMII CTCAG 5 cut(s) 45, 502, 519, 534, 584
BseNI ACTGG 2 cut(s) 202, 1015
BseRI GAGGAG 2 cut(s) 200, 335
BseXI GCAGC 2 cut(s) 823, 963
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 5 cut(s) 366, 692, 749, 1080, 1178
BsiHKAI GWGCWC 1 cut(s) 230
BsiSI CCGG 2 cut(s) 231, 1038
BslFI GGGAC 2 cut(s) 273, 988
BslI CCNNNNNNNGG 6 cut(s) 744, 745, 930, 1185, 1342, 1369
BsmAI GTCTC 3 cut(s) 75, 594, 1154
BsmFI GGGAC 2 cut(s) 273, 988
BsmI GAATGC 2 cut(s) 339, 1029
BsnI GGCC 5 cut(s) 366, 692, 749, 1080, 1178
Bso31I GGTCTC 1 cut(s) 75
Bsp1286I GDGCHC 2 cut(s) 230, 599
Bsp143I GATC 4 cut(s) 162, 261, 623, 1018
Bsp1720I GCTNAGC 1 cut(s) 505
BspACI CCGC 2 cut(s) 492, 1041
BspANI GGCC 5 cut(s) 366, 692, 749, 1080, 1178
BspCNI CTCAG 5 cut(s) 44, 501, 518, 533, 585
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 3 cut(s) 748, 1361, 1374
BspPI GGATC 2 cut(s) 269, 618
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 1291
BsrFI RCCGGY 1 cut(s) 1037
BsrI ACTGG 2 cut(s) 202, 1015
BssAI RCCGGY 1 cut(s) 1037
BssECI CCNNGG 2 cut(s) 257, 831
BssMI GATC 4 cut(s) 162, 261, 623, 1018
BssT1I CCWWGG 1 cut(s) 831
Bst4CI ACNGT 2 cut(s) 725, 1207
Bst6I CTCTTC 1 cut(s) 1178
BstC8I GCNNGC 4 cut(s) 242, 918, 1104, 1231
BstDEI CTNAG 8 cut(s) 11, 31, 488, 505, 520, 593, 863, 963
BstF5I GGATG 3 cut(s) 956, 1116, 1285
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 4 cut(s) 165, 264, 626, 1021
BstMAI GTCTC 3 cut(s) 75, 594, 1154
BstMBI GATC 4 cut(s) 162, 261, 623, 1018
BstMWI GCNNNNNNNGC 4 cut(s) 32, 799, 845, 1239
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 2 cut(s) 823, 963
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 623
BstXI CCANNNNNNTGG 2 cut(s) 184, 1127
BstYI RGATCY 1 cut(s) 623
Bsu36I CCTNAGG 1 cut(s) 863
BsuRI GGCC 5 cut(s) 366, 692, 749, 1080, 1178
BtsCI GGATG 3 cut(s) 956, 1116, 1285
BtsI GCAGTG 1 cut(s) 1056
BtsIMutI CAGTG 3 cut(s) 1056, 1083, 1212
Cac8I GCNNGC 4 cut(s) 242, 918, 1104, 1231
CfoI GCGC 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 1037
Cfr13I GGNCC 4 cut(s) 174, 690, 747, 1372
Csp6I GTAC 3 cut(s) 199, 607, 1312
CviQI GTAC 3 cut(s) 199, 607, 1312
DdeI CTNAG 8 cut(s) 11, 31, 488, 505, 520, 593, 863, 963
DpnI GATC 4 cut(s) 164, 263, 625, 1020
DpnII GATC 4 cut(s) 162, 261, 623, 1018
DraI TTTAAA 1 cut(s) 1237
DraIII CACNNNGTG 1 cut(s) 57
DrdI GACNNNNNNGTC 1 cut(s) 476
DseDI GACNNNNNNGTC 1 cut(s) 476
Eam1104I CTCTTC 1 cut(s) 1178
EarI CTCTTC 1 cut(s) 1178
EciI GGCGGA 1 cut(s) 1056
Ecl136II GAGCTC 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 831
Eco147I AGGCCT 1 cut(s) 366
Eco24I GRGCYC 2 cut(s) 230, 599
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 174, 1372
Eco53kI GAGCTC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 567
Eco81I CCTNAGG 1 cut(s) 863
EcoICRI GAGCTC 1 cut(s) 228
EcoO109I RGGNCCY 2 cut(s) 690, 1372
EcoT14I CCWWGG 1 cut(s) 831
EcoT38I GRGCYC 2 cut(s) 230, 599
ErhI CCWWGG 1 cut(s) 831
FalI AAGNNNNNCTT 2 cut(s) 786, 818
FaqI GGGAC 2 cut(s) 273, 988
FbaI TGATCA 1 cut(s) 1018
Fnu4HI GCNGC 2 cut(s) 837, 952
FokI GGATG 3 cut(s) 943, 1123, 1292
FriOI GRGCYC 2 cut(s) 230, 599
Fsp4HI GCNGC 2 cut(s) 837, 952
FspBI CTAG 2 cut(s) 194, 510
GlaI GCGC 1 cut(s) 255
GluI GCNGC 2 cut(s) 837, 952
GsuI CTGGAG 1 cut(s) 1277
HaeIII GGCC 5 cut(s) 366, 692, 749, 1080, 1178
HapII CCGG 2 cut(s) 231, 1038
HhaI GCGC 1 cut(s) 256
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HindIII AAGCTT 3 cut(s) 137, 1219, 1388
HinfI GANTC 6 cut(s) 281, 302, 587, 631, 758, 1072
HpaII CCGG 2 cut(s) 231, 1038
HphI GGTGA 3 cut(s) 83, 803, 1211
Hpy166II GTNNAC 4 cut(s) 17, 51, 609, 856
Hpy188I TCNGA 4 cut(s) 12, 345, 586, 594
Hpy188III TCNNGA 6 cut(s) 169, 194, 535, 1192, 1256, 1291
Hpy8I GTNNAC 4 cut(s) 17, 51, 609, 856
HpyAV CCTTC 4 cut(s) 340, 384, 435, 1189
HpyCH4III ACNGT 2 cut(s) 725, 1207
HpyCH4V TGCA 8 cut(s) 569, 638, 774, 920, 1029, 1106, 1142, 1242
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 799, 845, 1239
HpyF3I CTNAG 8 cut(s) 11, 31, 488, 505, 520, 593, 863, 963
HspAI GCGC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 1018
Kzo9I GATC 4 cut(s) 162, 261, 623, 1018
LmnI GCTCC 3 cut(s) 233, 537, 1365
Lsp1109I GCAGC 2 cut(s) 823, 963
MaeI CTAG 2 cut(s) 194, 510
MaeIII GTNAC 2 cut(s) 92, 577
MalI GATC 4 cut(s) 164, 263, 625, 1020
MboI GATC 4 cut(s) 162, 261, 623, 1018
MboII GAAGA 6 cut(s) 182, 311, 319, 797, 1195, 1406
MfeI CAATTG 1 cut(s) 601
MflI RGATCY 1 cut(s) 623
MhlI GDGCHC 2 cut(s) 230, 599
MluCI AATT 9 cut(s) 181, 381, 601, 640, 870, 935, 1062, 1302, 1349
MlyI GAGTC 3 cut(s) 596, 767, 1081
MmeI TCCRAC 2 cut(s) 333, 1387
MroXI GAANNNNTTC 1 cut(s) 1398
MseI TTAA 1 cut(s) 1236
MslI CAYNNNNRTG 1 cut(s) 314
MspA1I CMGCKG 2 cut(s) 492, 1248
MspI CCGG 2 cut(s) 231, 1038
MunI CAATTG 1 cut(s) 601
Mva1269I GAATGC 2 cut(s) 339, 1029
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 4 cut(s) 32, 799, 845, 1239
NdeII GATC 4 cut(s) 162, 261, 623, 1018
NlaIV GGNNCC 3 cut(s) 748, 1361, 1374
NmeAIII GCCGAG 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 577
PceI AGGCCT 1 cut(s) 366
PctI GAATGC 2 cut(s) 339, 1029
PdmI GAANNNNTTC 1 cut(s) 1398
PfeI GAWTC 3 cut(s) 281, 302, 631
PkrI GCNGC 2 cut(s) 838, 953
PleI GAGTC 3 cut(s) 595, 766, 1080
PpsI GAGTC 3 cut(s) 595, 766, 1080
PpuMI RGGWCCY 1 cut(s) 1372
PsiI TTATAA 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 1372
PspN4I GGNNCC 3 cut(s) 748, 1361, 1374
PspPI GGNCC 4 cut(s) 174, 690, 747, 1372
PspPPI RGGWCCY 1 cut(s) 1372
PsrI GAACNNNNNNTAC 2 cut(s) 32, 64
PsuI RGATCY 1 cut(s) 623
PvuII CAGCTG 1 cut(s) 1248
RsaI GTAC 3 cut(s) 200, 608, 1313
RsaNI GTAC 3 cut(s) 199, 607, 1312
RseI CAYNNNNRTG 1 cut(s) 314
SacI GAGCTC 1 cut(s) 230
SaqAI TTAA 1 cut(s) 1236
SatI GCNGC 2 cut(s) 837, 952
Sau3AI GATC 4 cut(s) 162, 261, 623, 1018
Sau96I GGNCC 4 cut(s) 174, 690, 747, 1372
SchI GAGTC 3 cut(s) 596, 767, 1081
SduI GDGCHC 2 cut(s) 230, 599
SgrAI CRCCGGYG 1 cut(s) 1037
SinI GGWCC 2 cut(s) 174, 1372
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 3 cut(s) 436, 1097, 1190
SmoI CTYRAG 3 cut(s) 436, 1097, 1190
Sse9I AATT 9 cut(s) 181, 381, 601, 640, 870, 935, 1062, 1302, 1349
SseBI AGGCCT 1 cut(s) 366
SsiI CCGC 2 cut(s) 492, 1041
SspI AATATT 1 cut(s) 562
SspMI CTAG 2 cut(s) 194, 510
SstI GAGCTC 1 cut(s) 230
StuI AGGCCT 1 cut(s) 366
StyI CCWWGG 1 cut(s) 831
TaaI ACNGT 2 cut(s) 725, 1207
TaqII GACCGA 1 cut(s) 147
TasI AATT 9 cut(s) 181, 381, 601, 640, 870, 935, 1062, 1302, 1349
TfiI GAWTC 3 cut(s) 281, 302, 631
Tru1I TTAA 1 cut(s) 1236
Tru9I TTAA 1 cut(s) 1236
TscAI CASTG 3 cut(s) 1056, 1090, 1212
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 2 cut(s) 836, 951
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 2 cut(s) 34, 1330
TspRI CASTG 3 cut(s) 1056, 1090, 1212
VpaK11BI GGWCC 2 cut(s) 174, 1372
XapI RAATTY 3 cut(s) 181, 870, 1349
XbaI TCTAGA 1 cut(s) 193
XcmI CCANNNNNNNNNTGG 2 cut(s) 898, 1339
XmnI GAANNNNTTC 1 cut(s) 1398
XspI CTAG 2 cut(s) 194, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.