MD03G1102500.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012947
Physical Location & Seq
Forward (+)
8771766 .. 8773298
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1102500.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGGGAGTCTTTAGCATCTTCACATGTAGTGTACCTCCTCCTCCTCCCTCTTCTTATAAGACTCCCATTTTCAAGCTCACATTTCCATCCACATCTATCTATCTATCTCTCTCTCCCCATTTTGTAATGGAGCTTCTTGCTTTAGTCTGTGTTTTTTCAGCCTTGTTCTTCATTTTTATGATGTGGAAGCTTTTTGATGAGAGGAGAGACCACGAGTGCTACATTTTGGACTACCAGTGCCACAAGCCCACAGATGACAGAAAGCTAGACACTGAGTACTCAGGTGAGATTATCAAGAGGACCAAGAATCTTGGCCTTTTGGAATACCAGTTCCTCCTCAAGGCAATTGTCAGCTCAGGGATTGGTGAGCAAACTTATGCTCCAAGAATCATGTTCTCAGGCAGGGAATCATGTCCCACATTGGAAGACAGCATCTCGGAGATGGAGGAGTTCTTCCATGACAGCATCAAGAAACTCCTTGACAAATCAGGGATTTCACCTTCAGAAATTGATGTGCTTGTTGTGAATGTCTCCATGTTTGCATCTGTTCCATCGTTGCCCTCCAGGATTATAAACCACTACAAGATGAGGGAGGACATAAAGGTCTTTAACCTCTGTGGGATGGGCTGCTCAGCATCCCTAATCTCAGTTGACATAGTGAAAAACATTTTCAAGTCACTCAAAAATGTTTATGCACTTGTGGTCACTTCTGAGTCCTTGAGCCCCAATTGGTATTCAGGCAATGCCAGATCCATGATTCTGGCAAACTGTTTGTTCAGGTCCGGCGGTTGCGCAATTCTACTGACCAACAAACGGGCCTTAAAACACCGAGCCATGTTCAAGTTGAAGTGCTTGGTGAGAACCCATCACGGTGCGAGAGACGATTCCTACGGGAGCTGCATCCAACAAGAAGATACACAAGGGAATCTAGGGGTTCACCTAGACAAATTCTTACCCAAGGCGGCTACGAGAGCTTTCGTGGACAACCTAAAGGTGATCTCCCCCAAGATTTTACCCATTAGGGAGCTGGTGAGGTTTTTGCTTGTAACCCTAGTGAAAAAAATGACCTCCTTCCAACATCCCACCAAGGGAGGGGCTACTTCTAGCCCATCACCAAAATCTGTGATCAATTTCAAAACTGGGGTGGATCATTTTTGCCTCCACACTGGTGGGAAGGCGGTGATCGATGGGGTAGGATTAAATCTTGGGCTGAGTGAGTATGATCTGGAACCTGCAAGGATGACACTCCACAGATTTGGGAACACATCAGCAAGCAGCCTCTGGTACGTGTTGGGATACATGGAGGCAAAGAAGAGGTTGAAGAAAGGAGATACAGTTTTGATGATAAGCTTCGGAGGTGGGTTTAAATGCAACAGTTGCTTGTGGGAGGTAGTGAGGGATTTGGGGGATGAAAATGTTTGGAAGGAGGACATTGCTGTGTACCCACCAAAGACACTCACAAACCCTTTCTTGGAGAAGTTTGGTTGGATTCATCAGGAGGATCCAAGCACATTCAACCTGGAAAATTATTAA

Protein Analysis

511

Amino Acids

57.36

Weight (kDa)

8.68

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 67 - 354 9.7e-116 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 382 - 463 1.7e-13 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 385 - 460 3.8e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07720 AT1G07720 AT2G28630 AT2G28630
fragaria_vesca FvH4_3g34690
malus_domestica MD03G1102500.v1.1 MD11G1116500.v1.1
prunus_persica Prupe.6G086800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0062381
rosa_laevigata RLG00000035554
rosa_multiflora Rmu_sc0005798.1_g000006
rosa_roxburghii Rroxscaffold_1G00018280
rosa_rugosa Rorug05G0349200
rosa_samantha Rh5AG408700 Rh5BG423100 Rh5CG447000 Rh5DG437800
rosa_wichuraiana Rw5G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 57, 572
AasI GACNNNNNNGTC 1 cut(s) 602
Acc16I TGCGCA 1 cut(s) 793
Acc36I ACCTGC 1 cut(s) 1240
AciI CCGC 3 cut(s) 786, 962, 1178
AclWI GGATC 4 cut(s) 744, 1155, 1496, 1509
AcsI RAATTY 1 cut(s) 947
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 4 cut(s) 33, 278, 1286, 1442
AfiI CCNNNNNNNGG 5 cut(s) 340, 813, 1088, 1092, 1471
AflIII ACRYGT 2 cut(s) 23, 1287
AgsI TTSAA 7 cut(s) 73, 673, 841, 847, 1135, 1321, 1516
AjnI CCWGG 2 cut(s) 563, 1518
AleI CACNNNNGTG 1 cut(s) 1167
AloI GAACNNNNNNTCC 2 cut(s) 314, 346
AluBI AGCT 9 cut(s) 76, 133, 190, 265, 354, 897, 974, 1027, 1350
AluI AGCT 9 cut(s) 76, 133, 190, 265, 354, 897, 974, 1027, 1350
Alw26I GTCTC 3 cut(s) 201, 535, 873
AlwI GGATC 4 cut(s) 744, 1155, 1496, 1509
AlwNI CAGNNNCTG 1 cut(s) 1281
AoxI GGCC 2 cut(s) 313, 816
ApeKI GCWGC 3 cut(s) 627, 897, 1275
ApoI RAATTY 1 cut(s) 947
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 3 cut(s) 300, 780, 816
AsuHPI GGTGA 9 cut(s) 296, 377, 489, 868, 929, 1006, 1042, 1104, 1192
AvaII GGWCC 2 cut(s) 300, 780
BamHI GGATCC 1 cut(s) 1501
BanII GRGCYC 1 cut(s) 725
BarI GAAGNNNNNNTAC 2 cut(s) 117, 149
BauI CACGAG 1 cut(s) 212
BbsI GAAGAC 1 cut(s) 432
BbvI GCAGC 3 cut(s) 614, 884, 1287
BccI CCATC 7 cut(s) 94, 436, 559, 616, 873, 1117, 1181
BciT130I CCWGG 2 cut(s) 565, 1520
BciVI GTATCC 1 cut(s) 1289
BclI TGATCA 1 cut(s) 1125
BcoDI GTCTC 3 cut(s) 201, 535, 873
BfaI CTAG 5 cut(s) 266, 929, 941, 1052, 1104
BfuAI ACCTGC 1 cut(s) 1240
BfuI GTATCC 1 cut(s) 1289
BisI GCNGC 4 cut(s) 628, 898, 963, 1276
BlpI GCTNAGC 1 cut(s) 631
BlsI GCNGC 4 cut(s) 629, 899, 964, 1277
BmcAI AGTACT 1 cut(s) 278
Bme1390I CCNGG 2 cut(s) 565, 1520
Bme18I GGWCC 2 cut(s) 300, 780
BmgT120I GGNCC 3 cut(s) 300, 780, 816
BmiI GGNNCC 2 cut(s) 1230, 1503
BmrFI CCNGG 2 cut(s) 565, 1520
BmrI ACTGGG 1 cut(s) 1149
BmsI GCATC 6 cut(s) 24, 441, 474, 551, 644, 909
BmuI ACTGGG 1 cut(s) 1149
BpiI GAAGAC 1 cut(s) 432
BpmI CTGGAG 1 cut(s) 547
Bpu10I CCTNAGC 1 cut(s) 355
Bpu1102I GCTNAGC 1 cut(s) 631
BpuEI CTTGAG 2 cut(s) 323, 739
Bsa29I ATCGAT 1 cut(s) 1185
BsaAI YACGTR 1 cut(s) 1288
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 957, 1086
BsaXI ACNNNNNCTCC 4 cut(s) 364, 394, 1320, 1350
Bsc4I CCNNNNNNNGG 5 cut(s) 340, 813, 1088, 1092, 1471
Bse1I ACTGG 4 cut(s) 235, 328, 1144, 1171
Bse3DI GCAATG 2 cut(s) 748, 1431
BseBI CCWGG 2 cut(s) 565, 1520
BseCI ATCGAT 1 cut(s) 1185
BseDI CCNNGG 2 cut(s) 957, 1086
BseGI GGATG 7 cut(s) 86, 627, 635, 900, 1078, 1245, 1414
BseLI CCNNNNNNNGG 5 cut(s) 340, 813, 1088, 1092, 1471
BseMI GCAATG 2 cut(s) 748, 1431
BseMII CTCAG 8 cut(s) 264, 294, 369, 411, 645, 660, 702, 1202
BseNI ACTGG 4 cut(s) 235, 328, 1144, 1171
BseRI GAGGAG 6 cut(s) 27, 30, 33, 217, 326, 461
BseXI GCAGC 3 cut(s) 614, 884, 1287
BshFI GGCC 2 cut(s) 315, 818
BshVI ATCGAT 1 cut(s) 1185
BsiSI CCGG 1 cut(s) 783
BslFI GGGAC 1 cut(s) 399
BslI CCNNNNNNNGG 5 cut(s) 340, 813, 1088, 1092, 1471
BsmAI GTCTC 3 cut(s) 201, 535, 873
BsmBI CGTCTC 1 cut(s) 873
BsmFI GGGAC 1 cut(s) 399
BsnI GGCC 2 cut(s) 315, 818
Bso31I GGTCTC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 725
Bsp143I GATC 7 cut(s) 749, 996, 1125, 1147, 1182, 1222, 1501
Bsp1720I GCTNAGC 1 cut(s) 631
BspACI CCGC 3 cut(s) 786, 962, 1178
BspANI GGCC 2 cut(s) 315, 818
BspCNI CTCAG 8 cut(s) 265, 293, 368, 410, 644, 659, 703, 1203
BspDI ATCGAT 1 cut(s) 1185
BspLI GGNNCC 2 cut(s) 1230, 1503
BspMI ACCTGC 1 cut(s) 1240
BspPI GGATC 4 cut(s) 744, 1155, 1496, 1509
BspTNI GGTCTC 1 cut(s) 201
BsrDI GCAATG 2 cut(s) 748, 1431
BsrI ACTGG 4 cut(s) 235, 328, 1144, 1171
BssECI CCNNGG 2 cut(s) 957, 1086
BssMI GATC 7 cut(s) 749, 996, 1125, 1147, 1182, 1222, 1501
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 2 cut(s) 957, 1086
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 2 cut(s) 565, 1520
Bst4CI ACNGT 4 cut(s) 770, 872, 1336, 1376
Bst6I CTCTTC 2 cut(s) 55, 1307
BstAPI GCANNNNNTGC 1 cut(s) 1377
BstBAI YACGTR 1 cut(s) 1288
BstC8I GCNNGC 1 cut(s) 1273
BstDEI CTNAG 8 cut(s) 273, 280, 355, 397, 631, 646, 711, 1211
BstENI CCTNNNNNAGG 1 cut(s) 338
BstF5I GGATG 7 cut(s) 86, 627, 635, 900, 1078, 1245, 1414
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 7 cut(s) 752, 999, 1128, 1150, 1185, 1225, 1504
BstMAI GTCTC 3 cut(s) 201, 535, 873
BstMBI GATC 7 cut(s) 749, 996, 1125, 1147, 1182, 1222, 1501
BstMWI GCNNNNNNNGC 2 cut(s) 971, 1377
BstNI CCWGG 2 cut(s) 565, 1520
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 2 cut(s) 563, 1518
BstV1I GCAGC 3 cut(s) 614, 884, 1287
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 2 cut(s) 749, 1501
BstXI CCANNNNNNTGG 3 cut(s) 760, 1169, 1256
BstYI RGATCY 2 cut(s) 749, 1501
Bsu15I ATCGAT 1 cut(s) 1185
BsuI GTATCC 1 cut(s) 1289
BsuRI GGCC 2 cut(s) 315, 818
BsuTUI ATCGAT 1 cut(s) 1185
BtsCI GGATG 7 cut(s) 86, 627, 635, 900, 1078, 1245, 1414
BtsIMutI CAGTG 3 cut(s) 242, 270, 1164
BveI ACCTGC 1 cut(s) 1240
Cac8I GCNNGC 1 cut(s) 1273
CaiI CAGNNNCTG 1 cut(s) 1281
CfoI GCGC 1 cut(s) 794
Cfr13I GGNCC 3 cut(s) 300, 780, 816
ClaI ATCGAT 1 cut(s) 1185
Csp6I GTAC 4 cut(s) 32, 277, 1285, 1441
CviAII CATG 8 cut(s) 24, 391, 411, 458, 535, 754, 835, 1300
CviQI GTAC 4 cut(s) 32, 277, 1285, 1441
DdeI CTNAG 8 cut(s) 273, 280, 355, 397, 631, 646, 711, 1211
DpnI GATC 7 cut(s) 751, 998, 1127, 1149, 1184, 1224, 1503
DpnII GATC 7 cut(s) 749, 996, 1125, 1147, 1182, 1222, 1501
DraI TTTAAA 1 cut(s) 1366
DrdI GACNNNNNNGTC 1 cut(s) 602
DseDI GACNNNNNNGTC 1 cut(s) 602
Eam1104I CTCTTC 2 cut(s) 55, 1307
EarI CTCTTC 2 cut(s) 55, 1307
Eco130I CCWWGG 2 cut(s) 957, 1086
Eco24I GRGCYC 1 cut(s) 725
Eco31I GGTCTC 1 cut(s) 201
Eco47I GGWCC 2 cut(s) 300, 780
Eco57I CTGAAG 1 cut(s) 486
EcoNI CCTNNNNNAGG 1 cut(s) 338
EcoRII CCWGG 2 cut(s) 563, 1518
EcoT14I CCWWGG 2 cut(s) 957, 1086
EcoT38I GRGCYC 1 cut(s) 725
ErhI CCWWGG 2 cut(s) 957, 1086
Esp3I CGTCTC 1 cut(s) 873
FaeI CATG 8 cut(s) 27, 394, 414, 461, 538, 757, 838, 1303
FaqI GGGAC 1 cut(s) 399
FatI CATG 8 cut(s) 23, 390, 410, 457, 534, 753, 834, 1299
FbaI TGATCA 1 cut(s) 1125
Fnu4HI GCNGC 4 cut(s) 628, 898, 963, 1276
FokI GGATG 7 cut(s) 73, 622, 634, 887, 1065, 1252, 1421
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 4 cut(s) 628, 898, 963, 1276
FspBI CTAG 5 cut(s) 266, 929, 941, 1052, 1104
FspI TGCGCA 1 cut(s) 793
GlaI GCGC 1 cut(s) 793
GluI GCNGC 4 cut(s) 628, 898, 963, 1276
GsuI CTGGAG 1 cut(s) 547
HaeIII GGCC 2 cut(s) 315, 818
HapII CCGG 1 cut(s) 783
HhaI GCGC 1 cut(s) 794
Hin1II CATG 8 cut(s) 27, 394, 414, 461, 538, 757, 838, 1303
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 2 cut(s) 188, 1348
HpaII CCGG 1 cut(s) 783
HphI GGTGA 9 cut(s) 296, 377, 489, 868, 929, 1006, 1042, 1104, 1192
Hpy166II GTNNAC 5 cut(s) 32, 652, 937, 982, 1441
Hpy188I TCNGA 4 cut(s) 439, 505, 712, 1355
Hpy188III TCNNGA 4 cut(s) 295, 469, 1226, 1496
Hpy8I GTNNAC 5 cut(s) 32, 652, 937, 982, 1441
HpyAV CCTTC 4 cut(s) 510, 1081, 1168, 1417
HpyCH4III ACNGT 4 cut(s) 770, 872, 1336, 1376
HpyCH4IV ACGT 1 cut(s) 1287
HpyCH4V TGCA 5 cut(s) 542, 695, 900, 1235, 1371
HpyF10VI GCNNNNNNNGC 2 cut(s) 971, 1377
HpyF3I CTNAG 8 cut(s) 273, 280, 355, 397, 631, 646, 711, 1211
HpySE526I ACGT 1 cut(s) 1287
Hsp92II CATG 8 cut(s) 27, 394, 414, 461, 538, 757, 838, 1303
HspAI GCGC 1 cut(s) 792
Ksp22I TGATCA 1 cut(s) 1125
Kzo9I GATC 7 cut(s) 749, 996, 1125, 1147, 1182, 1222, 1501
LmnI GCTCC 4 cut(s) 130, 385, 894, 1024
Lsp1109I GCAGC 3 cut(s) 614, 884, 1287
LweI GCATC 6 cut(s) 24, 441, 474, 551, 644, 909
MaeI CTAG 5 cut(s) 266, 929, 941, 1052, 1104
MaeII ACGT 1 cut(s) 1287
MaeIII GTNAC 3 cut(s) 675, 703, 1045
MalI GATC 7 cut(s) 751, 998, 1127, 1149, 1184, 1224, 1503
MboI GATC 7 cut(s) 749, 996, 1125, 1147, 1182, 1222, 1501
MboII GAAGA 8 cut(s) 10, 42, 160, 437, 445, 923, 1324, 1333
MfeI CAATTG 2 cut(s) 345, 727
MflI RGATCY 2 cut(s) 749, 1501
MhlI GDGCHC 1 cut(s) 725
MluCI AATT 7 cut(s) 345, 507, 727, 795, 947, 1129, 1525
MlyI GAGTC 3 cut(s) 15, 55, 722
MmeI TCCRAC 3 cut(s) 928, 1099, 1466
MseI TTAA 5 cut(s) 609, 821, 1199, 1365, 1531
MslI CAYNNNNRTG 4 cut(s) 176, 870, 1167, 1436
MspI CCGG 1 cut(s) 783
MspR9I CCNGG 2 cut(s) 565, 1520
MunI CAATTG 2 cut(s) 345, 727
MvaI CCWGG 2 cut(s) 565, 1520
MwoI GCNNNNNNNGC 2 cut(s) 971, 1377
NdeII GATC 7 cut(s) 749, 996, 1125, 1147, 1182, 1222, 1501
NlaIII CATG 8 cut(s) 27, 394, 414, 461, 538, 757, 838, 1303
NlaIV GGNNCC 2 cut(s) 1230, 1503
NmuCI GTSAC 2 cut(s) 675, 703
NsbI TGCGCA 1 cut(s) 793
NspI RCATGY 1 cut(s) 27
OliI CACNNNNGTG 1 cut(s) 1167
PciI ACATGT 1 cut(s) 23
PfeI GAWTC 7 cut(s) 307, 387, 407, 757, 884, 925, 1489
PfoI TCCNGGA 1 cut(s) 563
PkrI GCNGC 4 cut(s) 629, 899, 964, 1277
PleI GAGTC 3 cut(s) 14, 55, 721
PpsI GAGTC 3 cut(s) 14, 55, 721
Ppu21I YACGTR 1 cut(s) 1288
PscI ACATGT 1 cut(s) 23
PsiI TTATAA 2 cut(s) 57, 572
Psp6I CCWGG 2 cut(s) 563, 1518
PspGI CCWGG 2 cut(s) 563, 1518
PspN4I GGNNCC 2 cut(s) 1230, 1503
PspPI GGNCC 3 cut(s) 300, 780, 816
PstNI CAGNNNCTG 1 cut(s) 1281
PsuI RGATCY 2 cut(s) 749, 1501
RsaI GTAC 4 cut(s) 33, 278, 1286, 1442
RsaNI GTAC 4 cut(s) 32, 277, 1285, 1441
RseI CAYNNNNRTG 4 cut(s) 176, 870, 1167, 1436
SaqAI TTAA 5 cut(s) 609, 821, 1199, 1365, 1531
SatI GCNGC 4 cut(s) 628, 898, 963, 1276
Sau3AI GATC 7 cut(s) 749, 996, 1125, 1147, 1182, 1222, 1501
Sau96I GGNCC 3 cut(s) 300, 780, 816
ScaI AGTACT 1 cut(s) 278
SchI GAGTC 3 cut(s) 15, 55, 722
ScrFI CCNGG 2 cut(s) 565, 1520
SduI GDGCHC 1 cut(s) 725
SfaNI GCATC 6 cut(s) 24, 441, 474, 551, 644, 909
SinI GGWCC 2 cut(s) 300, 780
SmiMI CAYNNNNRTG 4 cut(s) 176, 870, 1167, 1436
SmlI CTYRAG 2 cut(s) 338, 718
SmoI CTYRAG 2 cut(s) 338, 718
Sse9I AATT 7 cut(s) 345, 507, 727, 795, 947, 1129, 1525
SsiI CCGC 3 cut(s) 786, 962, 1178
SspMI CTAG 5 cut(s) 266, 929, 941, 1052, 1104
StyD4I CCNGG 2 cut(s) 563, 1518
StyI CCWWGG 2 cut(s) 957, 1086
TaaI ACNGT 4 cut(s) 770, 872, 1336, 1376
TaiI ACGT 1 cut(s) 1290
TaqI TCGA 1 cut(s) 1185
TasI AATT 7 cut(s) 345, 507, 727, 795, 947, 1129, 1525
TatI WGTACW 1 cut(s) 276
TauI GCSGC 1 cut(s) 965
TfiI GAWTC 7 cut(s) 307, 387, 407, 757, 884, 925, 1489
Tru1I TTAA 5 cut(s) 609, 821, 1199, 1365, 1531
Tru9I TTAA 5 cut(s) 609, 821, 1199, 1365, 1531
TscAI CASTG 3 cut(s) 242, 277, 1171
TseFI GTSAC 2 cut(s) 675, 703
TseI GCWGC 3 cut(s) 627, 897, 1275
Tsp45I GTSAC 2 cut(s) 675, 703
TspDTI ATGAA 3 cut(s) 160, 1425, 1481
TspRI CASTG 3 cut(s) 242, 277, 1171
VpaK11BI GGWCC 2 cut(s) 300, 780
XagI CCTNNNNNAGG 1 cut(s) 338
XapI RAATTY 1 cut(s) 947
XceI RCATGY 1 cut(s) 27
XcmI CCANNNNNNNNNTGG 1 cut(s) 1024
XspI CTAG 5 cut(s) 266, 929, 941, 1052, 1104
ZrmI AGTACT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.