Prupe.6G086800_v2.0.a1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0012947
Physical Location & Seq
Forward (+)
5873392 .. 5875682
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G086800.1

Sequence Viewer

Length: 1545 bp
ATGTTGTGTACTCTTGCTCCTCCTCTTCTTATAAATCTTCCAATTTCACTTCCCATCTACATAAATTCACAGACAACTCACCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCATTTGGTCATGGAGCTTCTCAGTTTACTCTGTGCTTTCTCAGCCTTGTACTTCATTTTTATGATGTGGAAACGCTTTGATGAAAAAAGGGACCAAGAGTGCTACATTTTGGACTATCAGTGCCATAAGCCCTCAGATGACAGAAAGCTAGACACACGGTTTTCAGGGGAGGTCATCCGGAGGACCAAGAATCTTGGTCTTCTAGAGTACCAGTTCCTCCTCAAAGCCATTGTGAGCTCAGGCATTGGTGAACAAACCTATGCTCCAAGAATCATTTTTTCAGGCAGAGAATCTTGCCCCACATTGGAAGATAGCGTCTCAGAGATGGAGGAGTTTTTCCATGACAGCATTCAGAAGCTTCTGGACAGATCAGGCATTTCCCCTTCACAACTTGATGTGCTTGTTGTGAATGTGTCCATGTTTGCTTCTGTGCCTTCTTTAGCTTCCAGAATTATCAACCACTACAAGATGAGGGAGGACATAAAGGTCTTTAATCTCACTGGGATGGGTTGCTCTGCTAGCCTAATTTCAACCGACATTGTTAGGAACATTTTCAAGTCCTACAAGAATGTGTATGCACTTGTGGTCACTTCTGAGTCTTTGAGCCCCAATTGGTATTCAGGTAATGACAGATCCATGATTTTAGCAAATTGTTTGTTTAGGTCTGGTGGTTGTGCGGTCCTTTTGACCAACAAAAGGGCCTTGAAAAACCAAGCCATGTTCAAATTGAAGTGTTTGGTTAGAACCCATCATGGGGCTAGAGATGAGTCATATGGTTGTTGCATCCAACAAGAAGATGGGCAAGGGATGCTTGGTTTTCACCTTGACAAGATCTTACCCAAGGCTGCCACAAGAGCCTTTGTGGATAATCTTAGGGAAATTTCACCCAAGATTTTACCCATTAGGGAGTTGGTTAGGTTTATGCTTGTAACCCTAGTGAGAAAATTGAAGTCACAATCCTCCAAGGGAGGAGCAAGCACTTCACCAAAACCTGTCATCAATTTCAAGACAGGGGTTGATCATTTTTGCCTTCACACTGGTGGAAAGGCAGTCATAGATGGGATTGGGTTGAACCTTGGGCTTAGTGAGTATGATCTTGAGCCTGCAAGGATGACACTTCACAGATTTGGCAACACATCTGCAAGTAGCCTTTGGTATGTCTTGGGTTACATGGAAGCCAAGAAGAGGCTCAAGAAAGGTGACACAGTGTTGATGATAAGCTTTGGTGCTGGCTTTAAATGCAACAGCTGTTTGCTAGAGGTGGTGAGAGATTTGGGAGATGAAAATGTGTGGAAGGAGGACATTGCTGTGTACCCACCAAAATCATTGACCAACCCTTTCTTGGAGAAATATGGTTGGATCCATCAAGAGGATCTAAGCACATTCAACCCTGAAAATGTGGTCATCTAA

Protein Analysis

515

Amino Acids

57.68

Weight (kDa)

8.62

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07720 AT1G07720 AT2G28630 AT2G28630
fragaria_vesca FvH4_3g34690
malus_domestica MD03G1102500.v1.1 MD11G1116500.v1.1
prunus_persica Prupe.6G086800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0062381
rosa_laevigata RLG00000035554
rosa_multiflora Rmu_sc0005798.1_g000006
rosa_roxburghii Rroxscaffold_1G00018280
rosa_rugosa Rorug05G0349200
rosa_samantha Rh5AG408700 Rh5BG423100 Rh5CG447000 Rh5DG437800
rosa_wichuraiana Rw5G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 32
AasI GACNNNNNNGTC 1 cut(s) 620
AccIII TCCGGA 1 cut(s) 312
AciI CCGC 1 cut(s) 812
AclWI GGATC 4 cut(s) 762, 1489, 1502, 1515
AcsI RAATTY 2 cut(s) 64, 1014
AfaI GTAC 4 cut(s) 10, 185, 344, 1448
AfiI CCNNNNNNNGG 7 cut(s) 439, 831, 888, 889, 1320, 1477, 1504
AgsI TTSAA 9 cut(s) 666, 691, 841, 859, 865, 1084, 1141, 1207, 1522
AleI CACNNNNGTG 1 cut(s) 1173
AluBI AGCT 7 cut(s) 151, 283, 372, 493, 578, 1356, 1383
AluI AGCT 7 cut(s) 151, 283, 372, 493, 578, 1356, 1383
Alw21I GWGCWC 1 cut(s) 374
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 4 cut(s) 762, 1489, 1502, 1515
Aor13HI TCCGGA 1 cut(s) 312
AoxI GGCC 1 cut(s) 834
ApeKI GCWGC 1 cut(s) 980
ApoI RAATTY 2 cut(s) 64, 1014
Asp700I GAANNNNTTC 1 cut(s) 686
AspS9I GGNCC 4 cut(s) 226, 318, 814, 834
AsuHPI GGTGA 7 cut(s) 71, 395, 947, 1011, 1110, 1346, 1411
AsuNHI GCTAGC 1 cut(s) 653
AvaII GGWCC 3 cut(s) 226, 318, 814
BamHI GGATCC 1 cut(s) 1494
BanII GRGCYC 2 cut(s) 374, 743
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 374
BbvI GCAGC 1 cut(s) 967
BccI CCATC 7 cut(s) 62, 454, 634, 891, 926, 1187, 1506
BclI TGATCA 1 cut(s) 1153
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 6 cut(s) 284, 338, 654, 894, 1070, 1391
BglII AGATCT 1 cut(s) 966
BisI GCNGC 1 cut(s) 981
BlsI GCNGC 1 cut(s) 982
Bme18I GGWCC 3 cut(s) 226, 318, 814
BmgT120I GGNCC 4 cut(s) 226, 318, 814, 834
BmiI GGNNCC 2 cut(s) 227, 1496
BmrI ACTGGG 1 cut(s) 645
BmsI GCATC 2 cut(s) 927, 933
BmtI GCTAGC 1 cut(s) 657
BmuI ACTGGG 1 cut(s) 645
BpiI GAAGAC 1 cut(s) 326
Bpu10I CCTNAGC 1 cut(s) 373
BpuEI CTTGAG 2 cut(s) 1253, 1310
BsaJI CCNNGG 3 cut(s) 975, 1098, 1210
BsaWI WCCGGW 1 cut(s) 312
BsaXI ACNNNNNCTCC 3 cut(s) 31, 382, 412
Bsc4I CCNNNNNNNGG 7 cut(s) 439, 831, 888, 889, 1320, 1477, 1504
Bse1I ACTGG 3 cut(s) 346, 640, 1177
Bse3DI GCAATG 1 cut(s) 1437
BseAI TCCGGA 1 cut(s) 312
BseDI CCNNGG 3 cut(s) 975, 1098, 1210
BseGI GGATG 5 cut(s) 309, 645, 918, 948, 1251
BseLI CCNNNNNNNGG 7 cut(s) 439, 831, 888, 889, 1320, 1477, 1504
BseMI GCAATG 1 cut(s) 1437
BseMII CTCAG 6 cut(s) 169, 189, 282, 387, 468, 720
BseNI ACTGG 3 cut(s) 346, 640, 1177
BseRI GAGGAG 5 cut(s) 9, 12, 344, 479, 1119
BseXI GCAGC 1 cut(s) 967
BshFI GGCC 1 cut(s) 836
BsiHKAI GWGCWC 1 cut(s) 374
BsiSI CCGG 1 cut(s) 313
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 7 cut(s) 439, 831, 888, 889, 1320, 1477, 1504
BsmAI GTCTC 1 cut(s) 457
BsmBI CGTCTC 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 239
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 1 cut(s) 836
Bsp1286I GDGCHC 2 cut(s) 374, 743
Bsp13I TCCGGA 1 cut(s) 312
Bsp143I GATC 7 cut(s) 503, 767, 966, 1153, 1228, 1494, 1507
BspACI CCGC 1 cut(s) 812
BspANI GGCC 1 cut(s) 836
BspCNI CTCAG 6 cut(s) 168, 188, 281, 386, 467, 721
BspEI TCCGGA 1 cut(s) 312
BspLI GGNNCC 2 cut(s) 227, 1496
BspOI GCTAGC 1 cut(s) 657
BspPI GGATC 4 cut(s) 762, 1489, 1502, 1515
BsrDI GCAATG 1 cut(s) 1437
BsrI ACTGG 3 cut(s) 346, 640, 1177
BssECI CCNNGG 3 cut(s) 975, 1098, 1210
BssMI GATC 7 cut(s) 503, 767, 966, 1153, 1228, 1494, 1507
BssT1I CCWWGG 3 cut(s) 975, 1098, 1210
Bst4CI ACNGT 2 cut(s) 294, 1342
Bst6I CTCTTC 2 cut(s) 30, 1313
BstAPI GCANNNNNTGC 1 cut(s) 943
BstC8I GCNNGC 4 cut(s) 655, 1111, 1239, 1366
BstDEI CTNAG 9 cut(s) 155, 175, 268, 373, 454, 729, 1007, 1217, 1511
BstF5I GGATG 5 cut(s) 309, 645, 918, 948, 1251
BstKTI GATC 7 cut(s) 506, 770, 969, 1156, 1231, 1497, 1510
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 7 cut(s) 503, 767, 966, 1153, 1228, 1494, 1507
BstMWI GCNNNNNNNGC 5 cut(s) 176, 654, 943, 989, 1374
BstV1I GCAGC 1 cut(s) 967
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 4 cut(s) 767, 966, 1494, 1507
BstYI RGATCY 4 cut(s) 767, 966, 1494, 1507
BsuRI GGCC 1 cut(s) 836
BtsCI GGATG 5 cut(s) 309, 645, 918, 948, 1251
BtsIMutI CAGTG 4 cut(s) 260, 633, 1170, 1347
Cac8I GCNNGC 4 cut(s) 655, 1111, 1239, 1366
Cfr13I GGNCC 4 cut(s) 226, 318, 814, 834
CseI GACGC 1 cut(s) 439
Csp6I GTAC 4 cut(s) 9, 184, 343, 1447
CviAII CATG 7 cut(s) 145, 476, 553, 772, 853, 887, 1306
CviQI GTAC 4 cut(s) 9, 184, 343, 1447
DdeI CTNAG 9 cut(s) 155, 175, 268, 373, 454, 729, 1007, 1217, 1511
DpnI GATC 7 cut(s) 505, 769, 968, 1155, 1230, 1496, 1509
DpnII GATC 7 cut(s) 503, 767, 966, 1153, 1228, 1494, 1507
DraI TTTAAA 1 cut(s) 1372
DrdI GACNNNNNNGTC 1 cut(s) 620
DseDI GACNNNNNNGTC 1 cut(s) 620
Eam1104I CTCTTC 2 cut(s) 30, 1313
EarI CTCTTC 2 cut(s) 30, 1313
Ecl136II GAGCTC 1 cut(s) 372
Eco130I CCWWGG 3 cut(s) 975, 1098, 1210
Eco24I GRGCYC 2 cut(s) 374, 743
Eco47I GGWCC 3 cut(s) 226, 318, 814
Eco53kI GAGCTC 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 834
EcoT14I CCWWGG 3 cut(s) 975, 1098, 1210
EcoT38I GRGCYC 2 cut(s) 374, 743
ErhI CCWWGG 3 cut(s) 975, 1098, 1210
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 7 cut(s) 148, 479, 556, 775, 856, 890, 1309
FalI AAGNNNNNCTT 2 cut(s) 930, 962
FaqI GGGAC 1 cut(s) 239
FatI CATG 7 cut(s) 144, 475, 552, 771, 852, 886, 1305
FauNDI CATATG 1 cut(s) 907
FbaI TGATCA 1 cut(s) 1153
Fnu4HI GCNGC 1 cut(s) 981
FokI GGATG 5 cut(s) 296, 652, 905, 955, 1258
FriOI GRGCYC 2 cut(s) 374, 743
Fsp4HI GCNGC 1 cut(s) 981
FspBI CTAG 6 cut(s) 284, 338, 654, 894, 1070, 1391
GluI GCNGC 1 cut(s) 981
HaeIII GGCC 1 cut(s) 836
HapII CCGG 1 cut(s) 313
HgaI GACGC 1 cut(s) 439
Hin1II CATG 7 cut(s) 148, 479, 556, 775, 856, 890, 1309
HindIII AAGCTT 2 cut(s) 491, 1354
HinfI GANTC 5 cut(s) 325, 405, 425, 731, 902
HpaII CCGG 1 cut(s) 313
HphI GGTGA 7 cut(s) 71, 395, 947, 1011, 1110, 1346, 1411
Hpy166II GTNNAC 4 cut(s) 9, 161, 386, 1447
Hpy188I TCNGA 4 cut(s) 271, 457, 489, 730
Hpy188III TCNNGA 8 cut(s) 313, 338, 497, 582, 1141, 1232, 1327, 1502
Hpy8I GTNNAC 4 cut(s) 9, 161, 386, 1447
HpyAV CCTTC 4 cut(s) 528, 579, 1175, 1423
HpyCH4III ACNGT 2 cut(s) 294, 1342
HpyCH4V TGCA 5 cut(s) 713, 918, 1241, 1277, 1377
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 654, 943, 989, 1374
HpyF3I CTNAG 9 cut(s) 155, 175, 268, 373, 454, 729, 1007, 1217, 1511
Hsp92II CATG 7 cut(s) 148, 479, 556, 775, 856, 890, 1309
Kpn2I TCCGGA 1 cut(s) 312
Ksp22I TGATCA 1 cut(s) 1153
Kzo9I GATC 7 cut(s) 503, 767, 966, 1153, 1228, 1494, 1507
LmnI GCTCC 4 cut(s) 22, 148, 403, 1106
Lsp1109I GCAGC 1 cut(s) 967
LweI GCATC 2 cut(s) 927, 933
MaeI CTAG 6 cut(s) 284, 338, 654, 894, 1070, 1391
MaeIII GTNAC 5 cut(s) 721, 1063, 1086, 1301, 1334
MalI GATC 7 cut(s) 505, 769, 968, 1155, 1230, 1496, 1509
MboI GATC 7 cut(s) 503, 767, 966, 1153, 1228, 1494, 1507
MboII GAAGA 6 cut(s) 17, 29, 326, 455, 941, 1330
MfeI CAATTG 1 cut(s) 745
MflI RGATCY 4 cut(s) 767, 966, 1494, 1507
MhlI GDGCHC 2 cut(s) 374, 743
MlyI GAGTC 2 cut(s) 740, 911
MmeI TCCRAC 2 cut(s) 946, 1472
MroI TCCGGA 1 cut(s) 312
MroXI GAANNNNTTC 1 cut(s) 686
MseI TTAA 2 cut(s) 627, 1371
MslI CAYNNNNRTG 4 cut(s) 194, 638, 1173, 1442
MspA1I CMGCKG 1 cut(s) 1383
MspI CCGG 1 cut(s) 313
MunI CAATTG 1 cut(s) 745
Mva1269I GAATGC 1 cut(s) 483
MwoI GCNNNNNNNGC 5 cut(s) 176, 654, 943, 989, 1374
NdeI CATATG 1 cut(s) 907
NdeII GATC 7 cut(s) 503, 767, 966, 1153, 1228, 1494, 1507
NheI GCTAGC 1 cut(s) 653
NlaIII CATG 7 cut(s) 148, 479, 556, 775, 856, 890, 1309
NlaIV GGNNCC 2 cut(s) 227, 1496
NmuCI GTSAC 3 cut(s) 721, 1086, 1334
OliI CACNNNNGTG 1 cut(s) 1173
PctI GAATGC 1 cut(s) 483
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 3 cut(s) 325, 405, 425
PkrI GCNGC 1 cut(s) 982
PleI GAGTC 2 cut(s) 739, 910
PpsI GAGTC 2 cut(s) 739, 910
PsiI TTATAA 1 cut(s) 32
Psp124BI GAGCTC 1 cut(s) 374
PspN4I GGNNCC 2 cut(s) 227, 1496
PspPI GGNCC 4 cut(s) 226, 318, 814, 834
PsuI RGATCY 4 cut(s) 767, 966, 1494, 1507
PvuII CAGCTG 1 cut(s) 1383
RsaI GTAC 4 cut(s) 10, 185, 344, 1448
RsaNI GTAC 4 cut(s) 9, 184, 343, 1447
RseI CAYNNNNRTG 4 cut(s) 194, 638, 1173, 1442
SacI GAGCTC 1 cut(s) 374
SaqAI TTAA 2 cut(s) 627, 1371
SatI GCNGC 1 cut(s) 981
Sau3AI GATC 7 cut(s) 503, 767, 966, 1153, 1228, 1494, 1507
Sau96I GGNCC 4 cut(s) 226, 318, 814, 834
SchI GAGTC 2 cut(s) 740, 911
SduI GDGCHC 2 cut(s) 374, 743
SfaNI GCATC 2 cut(s) 927, 933
SinI GGWCC 3 cut(s) 226, 318, 814
SmiMI CAYNNNNRTG 4 cut(s) 194, 638, 1173, 1442
SmlI CTYRAG 2 cut(s) 1232, 1325
SmoI CTYRAG 2 cut(s) 1232, 1325
SsiI CCGC 1 cut(s) 812
SspMI CTAG 6 cut(s) 284, 338, 654, 894, 1070, 1391
SstI GAGCTC 1 cut(s) 374
StyI CCWWGG 3 cut(s) 975, 1098, 1210
TaaI ACNGT 2 cut(s) 294, 1342
TatI WGTACW 2 cut(s) 8, 183
TfiI GAWTC 3 cut(s) 325, 405, 425
Tru1I TTAA 2 cut(s) 627, 1371
Tru9I TTAA 2 cut(s) 627, 1371
TscAI CASTG 4 cut(s) 260, 640, 1177, 1347
TseFI GTSAC 3 cut(s) 721, 1086, 1334
TseI GCWGC 1 cut(s) 980
Tsp45I GTSAC 3 cut(s) 721, 1086, 1334
TspDTI ATGAA 3 cut(s) 178, 231, 1431
TspRI CASTG 4 cut(s) 260, 640, 1177, 1347
VpaK11BI GGWCC 3 cut(s) 226, 318, 814
XapI RAATTY 2 cut(s) 64, 1014
XbaI TCTAGA 1 cut(s) 337
XcmI CCANNNNNNNNNTGG 3 cut(s) 929, 1042, 1474
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 6 cut(s) 284, 338, 654, 894, 1070, 1391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.