FvH4_3g41280

Transmembrane protein 56-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004387
Physical Location & Seq
Reverse (-)
34706027 .. 34710207
4181 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41280.t4

Sequence Viewer

Length: 825 bp
ATGGTGGGAATTGCATATCCTCTTGGTTTTATGTCCATCAGTGGCTATGTCAGCCCAAACAAAGAGTTTCACTGGCTGGCTTCAGCTCTTTTTGGCATGCTTCTCTGCAGAATTGTTTACAAATTAACTGGTCTTATTAGCCTTCATAGCTTCAAGGGATATCAGAAACTGACCAAAGCAGAAAAAGTTGAATGGAACAACCGGGGTTTCTCAACTGTTCATGCTATTGGTGTAGCAGTTGCATCTTTCTACCTTATACTGTTATCAGATACATTTCATGAGGATTCTAGTGATGTGCTGATCATTAGTAGAAGATCTACTTTGTCAGATACAACATTGGGGATCTCCATTGGTTATTTTCTGTCAGACATAGCAATGATTTTTTGGCACTTTCCTGCTTTAGGTGGACTGGAGTATGTTTTGCACCATGGACTATCTATGTTCTCAATCTTCCTTTCCCTTATAAGTGGTGAAGGGCAGATTTACATACTAATGGTTCTGTTCTCTGAGAGCACAACTCCTTTCGTAAACCTAAGATGGTACTTGGACATTGCTGGTAAGAAGAACTCTAACCTGTACGTCTGCAATGGGATAGCATTGTTCCTGGGGTGGCTGATTGCAAGGATTCTTTTGTTCATTTACTTTTTCATCCACATGTTTTTCCATTTTGATCAAGTCAAGACAATTTTCCCATTGGGATTTTACAGCTTGCTCTCAATACCTCCTGTGTTGGCAATGATGAATTTGTTTTGGTTTTGGAAGATTGCCAAAGGTTTGATCAAAACAGTTTCGAAGGCAAAACACAAGAACATACACAGTCAGTGA

Protein Analysis

275

Amino Acids

31.16

Weight (kDa)

9.32

Isoelectric Point (pI)

31.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 64 - 257 5e-33 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
AclWI GGATC 1 cut(s) 350
AcsI RAATTY 1 cut(s) 742
AcuI CTGAAG 1 cut(s) 66
AfaI GTAC 2 cut(s) 542, 578
AfiI CCNNNNNNNGG 1 cut(s) 401
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 2 cut(s) 154, 191
AjnI CCWGG 1 cut(s) 603
AluBI AGCT 3 cut(s) 86, 150, 708
AluI AGCT 3 cut(s) 86, 150, 708
Alw21I GWGCWC 1 cut(s) 515
AlwI GGATC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 169
ApoI RAATTY 1 cut(s) 742
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 482
AsuII TTCGAA 1 cut(s) 791
Bbv12I GWGCWC 1 cut(s) 515
BccI CCATC 2 cut(s) 44, 531
BciT130I CCWGG 1 cut(s) 605
BclI TGATCA 3 cut(s) 300, 670, 777
BcnI CCSGG 1 cut(s) 203
BfaI CTAG 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 106
BglII AGATCT 1 cut(s) 314
Bme1390I CCNGG 2 cut(s) 203, 605
BmrFI CCNGG 2 cut(s) 203, 605
BmsI GCATC 1 cut(s) 251
BplI GAGNNNNNCTC 2 cut(s) 502, 534
BpmI CTGGAG 1 cut(s) 431
Bpu14I TTCGAA 1 cut(s) 791
BpuMI CCSGG 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 202, 427, 604
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse1I ACTGG 3 cut(s) 77, 133, 414
Bse3DI GCAATG 4 cut(s) 381, 549, 592, 741
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 3 cut(s) 202, 427, 604
BseGI GGATG 1 cut(s) 648
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 4 cut(s) 381, 549, 592, 741
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 3 cut(s) 77, 133, 414
BsiHKAI GWGCWC 1 cut(s) 515
BsiSI CCGG 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 401
Bsp119I TTCGAA 1 cut(s) 791
Bsp1286I GDGCHC 1 cut(s) 515
Bsp143I GATC 5 cut(s) 300, 314, 342, 670, 777
Bsp19I CCATGG 1 cut(s) 427
BspCNI CTCAG 1 cut(s) 499
BspHI TCATGA 1 cut(s) 277
BspMAI CTGCAG 1 cut(s) 110
BspPI GGATC 1 cut(s) 350
BspT104I TTCGAA 1 cut(s) 791
BsrDI GCAATG 4 cut(s) 381, 549, 592, 741
BsrI ACTGG 3 cut(s) 77, 133, 414
BssECI CCNNGG 3 cut(s) 202, 427, 604
BssMI GATC 5 cut(s) 300, 314, 342, 670, 777
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 4 cut(s) 217, 261, 787, 818
BstBI TTCGAA 1 cut(s) 791
BstC8I GCNNGC 3 cut(s) 78, 98, 710
BstDEI CTNAG 2 cut(s) 507, 533
BstDSI CCRYGG 1 cut(s) 427
BstENI CCTNNNNNAGG 1 cut(s) 399
BstF5I GGATG 1 cut(s) 648
BstKTI GATC 5 cut(s) 303, 317, 345, 673, 780
BstMBI GATC 5 cut(s) 300, 314, 342, 670, 777
BstMWI GCNNNNNNNGC 2 cut(s) 51, 147
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 2 cut(s) 100, 658
BstSCI CCNGG 2 cut(s) 201, 603
BstSFI CTRYAG 1 cut(s) 106
BstX2I RGATCY 2 cut(s) 314, 342
BstYI RGATCY 2 cut(s) 314, 342
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 1 cut(s) 648
BtsIMutI CAGTG 2 cut(s) 46, 70
Cac8I GCNNGC 3 cut(s) 78, 98, 710
CaiI CAGNNNCTG 1 cut(s) 169
CciI TCATGA 1 cut(s) 277
Csp6I GTAC 2 cut(s) 541, 577
CviAII CATG 5 cut(s) 97, 221, 278, 428, 655
CviJI RGCY 9 cut(s) 45, 54, 76, 80, 86, 141, 150, 613, 708
CviKI_1 RGCY 9 cut(s) 45, 54, 76, 80, 86, 141, 150, 613, 708
CviQI GTAC 2 cut(s) 541, 577
DdeI CTNAG 2 cut(s) 507, 533
DpnI GATC 5 cut(s) 302, 316, 344, 672, 779
DpnII GATC 5 cut(s) 300, 314, 342, 670, 777
Eco130I CCWWGG 1 cut(s) 427
Eco32I GATATC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 66
EcoNI CCTNNNNNAGG 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 603
EcoRV GATATC 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 5 cut(s) 100, 224, 281, 431, 658
FalI AAGNNNNNCTT 2 cut(s) 304, 336
FatI CATG 5 cut(s) 96, 220, 277, 427, 654
FbaI TGATCA 3 cut(s) 300, 670, 777
FokI GGATG 1 cut(s) 635
FspBI CTAG 1 cut(s) 288
GsuI CTGGAG 1 cut(s) 431
HapII CCGG 1 cut(s) 202
Hin1II CATG 5 cut(s) 100, 224, 281, 431, 658
HinfI GANTC 2 cut(s) 284, 625
HpaII CCGG 1 cut(s) 202
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 3 cut(s) 118, 407, 529
Hpy188I TCNGA 5 cut(s) 165, 268, 328, 367, 508
Hpy188III TCNNGA 2 cut(s) 278, 679
Hpy8I GTNNAC 3 cut(s) 118, 407, 529
HpyAV CCTTC 3 cut(s) 152, 467, 787
HpyCH4III ACNGT 4 cut(s) 217, 261, 787, 818
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 6 cut(s) 14, 108, 242, 424, 585, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 147
HpyF3I CTNAG 2 cut(s) 507, 533
HpySE526I ACGT 1 cut(s) 579
Hsp92II CATG 5 cut(s) 100, 224, 281, 431, 658
Ksp22I TGATCA 3 cut(s) 300, 670, 777
Kzo9I GATC 5 cut(s) 300, 314, 342, 670, 777
LweI GCATC 1 cut(s) 251
MaeI CTAG 1 cut(s) 288
MaeII ACGT 1 cut(s) 579
MalI GATC 5 cut(s) 302, 316, 344, 672, 779
MboI GATC 5 cut(s) 300, 314, 342, 670, 777
MboII GAAGA 4 cut(s) 324, 442, 574, 772
MflI RGATCY 2 cut(s) 314, 342
MhlI GDGCHC 1 cut(s) 515
MluCI AATT 5 cut(s) 9, 111, 122, 684, 742
MnlI CCTC 3 cut(s) 30, 274, 732
MseI TTAA 1 cut(s) 125
MslI CAYNNNNRTG 3 cut(s) 374, 491, 653
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 2 cut(s) 203, 605
MvaI CCWGG 1 cut(s) 605
MwoI GCNNNNNNNGC 2 cut(s) 51, 147
NciI CCSGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 427
NdeII GATC 5 cut(s) 300, 314, 342, 670, 777
NlaIII CATG 5 cut(s) 100, 224, 281, 431, 658
NspI RCATGY 2 cut(s) 100, 658
NspV TTCGAA 1 cut(s) 791
PaeI GCATGC 1 cut(s) 100
PagI TCATGA 1 cut(s) 277
PciI ACATGT 1 cut(s) 654
PfeI GAWTC 2 cut(s) 284, 625
PscI ACATGT 1 cut(s) 654
PsiI TTATAA 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PstI CTGCAG 1 cut(s) 110
PstNI CAGNNNCTG 1 cut(s) 169
PsuI RGATCY 2 cut(s) 314, 342
RsaI GTAC 2 cut(s) 542, 578
RsaNI GTAC 2 cut(s) 541, 577
RseI CAYNNNNRTG 3 cut(s) 374, 491, 653
SaqAI TTAA 1 cut(s) 125
Sau3AI GATC 5 cut(s) 300, 314, 342, 670, 777
ScrFI CCNGG 2 cut(s) 203, 605
SduI GDGCHC 1 cut(s) 515
SfaNI GCATC 1 cut(s) 251
SfcI CTRYAG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 791
SmiMI CAYNNNNRTG 3 cut(s) 374, 491, 653
SphI GCATGC 1 cut(s) 100
Sse9I AATT 5 cut(s) 9, 111, 122, 684, 742
SspMI CTAG 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 201, 603
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 4 cut(s) 217, 261, 787, 818
TaiI ACGT 1 cut(s) 582
TaqI TCGA 1 cut(s) 791
TasI AATT 5 cut(s) 9, 111, 122, 684, 742
TfiI GAWTC 2 cut(s) 284, 625
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 2 cut(s) 46, 77
TspDTI ATGAA 6 cut(s) 134, 209, 266, 625, 637, 755
TspRI CASTG 2 cut(s) 46, 77
XagI CCTNNNNNAGG 1 cut(s) 399
XapI RAATTY 1 cut(s) 742
XceI RCATGY 2 cut(s) 100, 658
XspI CTAG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.