FvH4_3g45810
WRKY Family

WRKY DNA -binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018827
Physical Location & Seq
Reverse (-)
38000582 .. 38002084
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45810.t1

Sequence Viewer

Length: 414 bp
ATGAAAACTGATCATCATGATGAGGATGAGGGAGATGGAGAAGGAGAAGAGGAGGTGCAAGACAACAACAATTCAGGCGGAAGCAACAAAGTGGTGATACCAGAAGATGGCTTCGAGTGGAAAAAATATGGACAGAAGTTTATTAAAAATGTTGGAAAGTTCAGAAGCTATTTCAAGTGCCAAAACAGCAATTGTAGAGCCAAGAAGCGAGCCGAGTGGTCGAGCTCAGAGCCTGATAAACTTCGAGTAGTGTATGAAGGGGTGCACAATCACACTGATAATAATGCCTCATCTGCAGGATCATCGACATCACATTCCTCATCATCTTTAAATCCGAGTGCTGATGCAAACCAATATGACTTGTACACACAAGTTTTTGGTAACCAATCCTCCCATTGGGATAAAAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

138

Amino Acids

15.37

Weight (kDa)

5.64

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 35 - 91 1.4e-20 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018827)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45810
rosa_chinensis RchiOBHm_Chr5g0083891
rosa_laevigata RLG00000037062
rosa_multiflora Rmu_sc0001137.1_g000010
rosa_rugosa Rorug05G0487300 Rorug05G0487300
rosa_samantha Rh5CG587100 Rh5DG573700
rosa_wichuraiana Rw5G050220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 107
AciI CCGC 1 cut(s) 78
AclWI GGATC 1 cut(s) 307
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 2 cut(s) 107, 396
AgsI TTSAA 1 cut(s) 175
AluBI AGCT 2 cut(s) 168, 225
AluI AGCT 2 cut(s) 168, 225
Alw21I GWGCWC 2 cut(s) 227, 267
Alw44I GTGCAC 1 cut(s) 263
AlwI GGATC 1 cut(s) 307
AlwNI CAGNNNCTG 1 cut(s) 233
ApaLI GTGCAC 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 227
Bbv12I GWGCWC 2 cut(s) 227, 267
BccI CCATC 2 cut(s) 29, 101
BcgI CGANNNNNNTGC 2 cut(s) 285, 319
BclI TGATCA 1 cut(s) 10
BfmI CTRYAG 1 cut(s) 294
BmsI GCATC 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 396
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 2 cut(s) 107, 396
BseMII CTCAG 1 cut(s) 240
BseRI GAGGAG 1 cut(s) 65
BseSI GKGCMC 1 cut(s) 267
BsiHKAI GWGCWC 2 cut(s) 227, 267
BslI CCNNNNNNNGG 2 cut(s) 107, 396
Bsp1286I GDGCHC 2 cut(s) 227, 267
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 2 cut(s) 10, 299
BspACI CCGC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 239
BspHI TCATGA 1 cut(s) 16
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 1 cut(s) 307
BsrGI TGTACA 1 cut(s) 363
BssMI GATC 2 cut(s) 10, 299
Bst6I CTCTTC 1 cut(s) 42
BstAUI TGTACA 1 cut(s) 363
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 1 cut(s) 226
BstEII GGTNACC 1 cut(s) 380
BstF5I GGATG 1 cut(s) 31
BstKTI GATC 2 cut(s) 13, 302
BstMBI GATC 2 cut(s) 10, 299
BstMWI GCNNNNNNNGC 2 cut(s) 186, 293
BstPI GGTNACC 1 cut(s) 380
BstSFI CTRYAG 1 cut(s) 294
BstSLI GKGCMC 1 cut(s) 267
BtsCI GGATG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 210
CaiI CAGNNNCTG 1 cut(s) 233
CciI TCATGA 1 cut(s) 16
Csp6I GTAC 1 cut(s) 364
CviAII CATG 1 cut(s) 17
CviJI RGCY 6 cut(s) 111, 168, 200, 212, 225, 232
CviKI_1 RGCY 6 cut(s) 111, 168, 200, 212, 225, 232
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 226
DpnI GATC 2 cut(s) 12, 301
DpnII GATC 2 cut(s) 10, 299
DraI TTTAAA 1 cut(s) 330
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
EciI GGCGGA 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 227
Eco53kI GAGCTC 1 cut(s) 225
Eco91I GGTNACC 1 cut(s) 380
EcoICRI GAGCTC 1 cut(s) 225
EcoO65I GGTNACC 1 cut(s) 380
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 1 cut(s) 20
FaiI YATR 4 cut(s) 18, 129, 255, 357
FatI CATG 1 cut(s) 16
FbaI TGATCA 1 cut(s) 10
FokI GGATG 1 cut(s) 38
FriOI GRGCYC 1 cut(s) 227
Hin1II CATG 1 cut(s) 20
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 265, 366
Hpy188I TCNGA 3 cut(s) 164, 229, 336
Hpy188III TCNNGA 1 cut(s) 17
Hpy8I GTNNAC 2 cut(s) 265, 366
HpyAV CCTTC 2 cut(s) 35, 251
HpyCH4V TGCA 4 cut(s) 58, 265, 296, 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 293
HpyF3I CTNAG 1 cut(s) 226
Hsp92II CATG 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 2 cut(s) 10, 299
LpnPI CCDG 4 cut(s) 60, 114, 246, 282
LweI GCATC 1 cut(s) 334
MaeIII GTNAC 1 cut(s) 380
MalI GATC 2 cut(s) 12, 301
MboI GATC 2 cut(s) 10, 299
MboII GAAGA 2 cut(s) 59, 116
MfeI CAATTG 1 cut(s) 190
MhlI GDGCHC 2 cut(s) 227, 267
MluCI AATT 2 cut(s) 70, 190
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 7 cut(s) 16, 22, 43, 46, 298, 328, 400
MseI TTAA 2 cut(s) 144, 329
MslI CAYNNNNRTG 1 cut(s) 18
MunI CAATTG 1 cut(s) 190
MwoI GCNNNNNNNGC 2 cut(s) 186, 293
NdeII GATC 2 cut(s) 10, 299
NlaIII CATG 1 cut(s) 20
NmeAIII GCCGAG 1 cut(s) 238
PagI TCATGA 1 cut(s) 16
PflMI CCANNNNNTGG 1 cut(s) 107
Psp124BI GAGCTC 1 cut(s) 227
PspEI GGTNACC 1 cut(s) 380
PstI CTGCAG 1 cut(s) 298
PstNI CAGNNNCTG 1 cut(s) 233
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 18
SacI GAGCTC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 144, 329
Sau3AI GATC 2 cut(s) 10, 299
SduI GDGCHC 2 cut(s) 227, 267
SetI ASST 3 cut(s) 57, 170, 227
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 18
Sse9I AATT 2 cut(s) 70, 190
SsiI CCGC 1 cut(s) 78
SstI GAGCTC 1 cut(s) 227
TaqI TCGA 4 cut(s) 114, 221, 244, 305
TasI AATT 2 cut(s) 70, 190
TatI WGTACW 1 cut(s) 363
Tru1I TTAA 2 cut(s) 144, 329
Tru9I TTAA 2 cut(s) 144, 329
TscAI CASTG 1 cut(s) 280
TspDTI ATGAA 2 cut(s) 17, 270
TspRI CASTG 1 cut(s) 280
Van91I CCANNNNNTGG 1 cut(s) 107
VneI GTGCAC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.