Rmu_sc0001137.1_g000010
WRKY Family

WRKY DNA -binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001137.1
OG0018827
Physical Location & Seq
Forward (+)
68121 .. 70335
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001137.1_g000010.1.cds

Sequence Viewer

Length: 648 bp
atgtccgattatcgattgattttatatagggaaactgatcatcatgatgaggatgatcagatatacggagaaggagaggaggtgcaagacaacaccagctcaggcggaagcaacaaattggtgataccagaagatggctacgaatggaaaaaatatggacaaaagttcattaaaaatgttggaaagttcaggagctatttcaagtgccaaaacagcaattgtagagccaagaagcgagccgagtggtcgagctcagagcctgatgaacttcgagtagtgtatgaaggggtgcacaatcacaccgataataatgcctcatctgcaggatcggcttcacattcacagcagccggattcctcatcatctttaaatccgagtactgatgcaaaccggtatgacttgtacacacaagtttttgccatgacttcagttggctcattggctgctcccagtggccgtgtcggcgctgataagaaatttgacaccttgtcgtctcgtgcttccatttcttcattctcattctccaagaggcccaatgtagctctcaggaggacacgttcccctagagtctccgccatggccaaggagttgcatttcaacaaggatggttctgccatcaagaagctacaagtgagtctttttgaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

215

Amino Acids

24.04

Weight (kDa)

8.42

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018827)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45810
rosa_chinensis RchiOBHm_Chr5g0083891
rosa_laevigata RLG00000037062
rosa_multiflora Rmu_sc0001137.1_g000010
rosa_rugosa Rorug05G0487300 Rorug05G0487300
rosa_samantha Rh5CG587100 Rh5DG573700
rosa_wichuraiana Rw5G050220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 134
AciI CCGC 2 cut(s) 105, 573
AclWI GGATC 1 cut(s) 334
AcoI YGGCCR 2 cut(s) 454, 579
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 2 cut(s) 379, 404
AfiI CCNNNNNNNGG 1 cut(s) 134
AflIII ACRYGT 1 cut(s) 554
AgeI ACCGGT 1 cut(s) 390
AgsI TTSAA 3 cut(s) 202, 598, 644
AhdI GACNNNNNGTC 1 cut(s) 487
AloI GAACNNNNNNTCC 2 cut(s) 541, 573
AluBI AGCT 5 cut(s) 99, 195, 252, 542, 625
AluI AGCT 5 cut(s) 99, 195, 252, 542, 625
Alw21I GWGCWC 2 cut(s) 254, 294
Alw26I GTCTC 2 cut(s) 498, 574
Alw44I GTGCAC 1 cut(s) 290
AlwI GGATC 1 cut(s) 334
AlwNI CAGNNNCTG 1 cut(s) 260
AoxI GGCC 3 cut(s) 454, 530, 579
ApaLI GTGCAC 1 cut(s) 290
ApeKI GCWGC 2 cut(s) 346, 443
ApoI RAATTY 1 cut(s) 476
AsiGI ACCGGT 1 cut(s) 390
AspLEI GCGC 1 cut(s) 467
AspS9I GGNCC 1 cut(s) 531
AsuHPI GGTGA 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 294
BalI TGGCCA 1 cut(s) 581
BanII GRGCYC 1 cut(s) 254
BauI CACGAG 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 254, 294
BbvI GCAGC 2 cut(s) 358, 430
BccI CCATC 3 cut(s) 128, 599, 623
BceAI ACGGC 1 cut(s) 441
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BclI TGATCA 2 cut(s) 37, 55
BcoDI GTCTC 2 cut(s) 498, 574
BfaI CTAG 1 cut(s) 564
BfmI CTRYAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 468
BglI GCCNNNNNGGC 1 cut(s) 462
BisI GCNGC 2 cut(s) 347, 444
BlsI GCNGC 2 cut(s) 348, 445
BmcAI AGTACT 1 cut(s) 379
BmeRI GACNNNNNGTC 1 cut(s) 487
BmgT120I GGNCC 1 cut(s) 531
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 1 cut(s) 373
BmuI ACTGGG 1 cut(s) 444
Bpu10I CCTNAGC 1 cut(s) 100
Bsa29I ATCGAT 1 cut(s) 13
BsaJI CCNNGG 2 cut(s) 576, 582
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 541, 571
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse118I RCCGGY 1 cut(s) 390
Bse1I ACTGG 1 cut(s) 450
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 2 cut(s) 576, 582
BseGI GGATG 2 cut(s) 58, 610
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 3 cut(s) 114, 267, 559
BseNI ACTGG 1 cut(s) 450
BseRI GAGGAG 1 cut(s) 92
BseSI GKGCMC 1 cut(s) 294
BseXI GCAGC 2 cut(s) 358, 430
BshFI GGCC 3 cut(s) 456, 532, 581
BshTI ACCGGT 1 cut(s) 390
BshVI ATCGAT 1 cut(s) 13
BsiHKAI GWGCWC 2 cut(s) 254, 294
BsiSI CCGG 2 cut(s) 350, 391
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 2 cut(s) 498, 574
BsmBI CGTCTC 1 cut(s) 498
BsnI GGCC 3 cut(s) 456, 532, 581
Bsp1286I GDGCHC 2 cut(s) 254, 294
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 3 cut(s) 37, 55, 326
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 2 cut(s) 105, 573
BspANI GGCC 3 cut(s) 456, 532, 581
BspCNI CTCAG 3 cut(s) 113, 266, 558
BspDI ATCGAT 1 cut(s) 13
BspHI TCATGA 1 cut(s) 43
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 390
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 1 cut(s) 450
BssAI RCCGGY 1 cut(s) 390
BssECI CCNNGG 2 cut(s) 576, 582
BssMI GATC 3 cut(s) 37, 55, 326
BssSI CACGAG 1 cut(s) 495
BssT1I CCWWGG 2 cut(s) 576, 582
Bst2BI CACGAG 1 cut(s) 495
BstAUI TGTACA 1 cut(s) 402
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 3 cut(s) 100, 253, 545
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 2 cut(s) 58, 610
BstH2I RGCGCY 1 cut(s) 468
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 3 cut(s) 40, 58, 329
BstMAI GTCTC 2 cut(s) 498, 574
BstMBI GATC 3 cut(s) 37, 55, 326
BstMWI GCNNNNNNNGC 4 cut(s) 213, 320, 329, 462
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 294
BstV1I GCAGC 2 cut(s) 358, 430
Bsu15I ATCGAT 1 cut(s) 13
BsuRI GGCC 3 cut(s) 456, 532, 581
BsuTUI ATCGAT 1 cut(s) 13
BtgI CCRYGG 1 cut(s) 576
BtsCI GGATG 2 cut(s) 58, 610
BtsIMutI CAGTG 1 cut(s) 457
Cac8I GCNNGC 1 cut(s) 237
CaiI CAGNNNCTG 1 cut(s) 260
CciI TCATGA 1 cut(s) 43
CfoI GCGC 1 cut(s) 467
Cfr10I RCCGGY 1 cut(s) 390
Cfr13I GGNCC 1 cut(s) 531
ClaI ATCGAT 1 cut(s) 13
Csp6I GTAC 2 cut(s) 378, 403
CspAI ACCGGT 1 cut(s) 390
CviAII CATG 3 cut(s) 44, 421, 577
CviQI GTAC 2 cut(s) 378, 403
DdeI CTNAG 3 cut(s) 100, 253, 545
DpnI GATC 3 cut(s) 39, 57, 328
DpnII GATC 3 cut(s) 37, 55, 326
DraI TTTAAA 1 cut(s) 369
DriI GACNNNNNGTC 1 cut(s) 487
EaeI YGGCCR 2 cut(s) 454, 579
Eam1105I GACNNNNNGTC 1 cut(s) 487
EciI GGCGGA 2 cut(s) 120, 562
Ecl136II GAGCTC 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 576, 582
Eco24I GRGCYC 1 cut(s) 254
Eco53kI GAGCTC 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 252
EcoT14I CCWWGG 2 cut(s) 576, 582
EcoT38I GRGCYC 1 cut(s) 254
ErhI CCWWGG 2 cut(s) 576, 582
Esp3I CGTCTC 1 cut(s) 498
FaeI CATG 3 cut(s) 47, 424, 580
FaiI YATR 9 cut(s) 25, 27, 45, 64, 156, 282, 396, 422, 578
FalI AAGNNNNNCTT 1 cut(s) 621
FatI CATG 3 cut(s) 43, 420, 576
FbaI TGATCA 2 cut(s) 37, 55
Fnu4HI GCNGC 2 cut(s) 347, 444
FokI GGATG 2 cut(s) 65, 617
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 2 cut(s) 347, 444
FspBI CTAG 1 cut(s) 564
GlaI GCGC 1 cut(s) 466
GluI GCNGC 2 cut(s) 347, 444
HaeII RGCGCY 1 cut(s) 468
HaeIII GGCC 3 cut(s) 456, 532, 581
HapII CCGG 2 cut(s) 350, 391
HhaI GCGC 1 cut(s) 467
Hin1II CATG 3 cut(s) 47, 424, 580
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HinfI GANTC 3 cut(s) 353, 567, 634
HpaII CCGG 2 cut(s) 350, 391
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 2 cut(s) 292, 405
Hpy188I TCNGA 4 cut(s) 7, 60, 256, 375
Hpy188III TCNNGA 4 cut(s) 44, 190, 547, 619
Hpy8I GTNNAC 2 cut(s) 292, 405
HpyAV CCTTC 2 cut(s) 65, 278
HpyCH4IV ACGT 1 cut(s) 556
HpyCH4V TGCA 5 cut(s) 85, 292, 323, 386, 592
HpyF10VI GCNNNNNNNGC 4 cut(s) 213, 320, 329, 462
HpyF3I CTNAG 3 cut(s) 100, 253, 545
HpySE526I ACGT 1 cut(s) 556
Hsp92II CATG 3 cut(s) 47, 424, 580
HspAI GCGC 1 cut(s) 465
Ksp22I TGATCA 2 cut(s) 37, 55
Kzo9I GATC 3 cut(s) 37, 55, 326
LmnI GCTCC 2 cut(s) 192, 451
Lsp1109I GCAGC 2 cut(s) 358, 430
LweI GCATC 1 cut(s) 373
MaeI CTAG 1 cut(s) 564
MaeII ACGT 1 cut(s) 556
MalI GATC 3 cut(s) 39, 57, 328
MboI GATC 3 cut(s) 37, 55, 326
MboII GAAGA 2 cut(s) 143, 501
MfeI CAATTG 1 cut(s) 217
MhlI GDGCHC 2 cut(s) 254, 294
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 3 cut(s) 116, 217, 476
MluNI TGGCCA 1 cut(s) 581
MlyI GAGTC 2 cut(s) 576, 643
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 7 cut(s) 43, 70, 73, 325, 367, 522, 543
Mox20I TGGCCA 1 cut(s) 581
MscI TGGCCA 1 cut(s) 581
MseI TTAA 2 cut(s) 171, 368
MslI CAYNNNNRTG 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 581
MspI CCGG 2 cut(s) 350, 391
MunI CAATTG 1 cut(s) 217
MwoI GCNNNNNNNGC 4 cut(s) 213, 320, 329, 462
NcoI CCATGG 1 cut(s) 576
NdeII GATC 3 cut(s) 37, 55, 326
NlaIII CATG 3 cut(s) 47, 424, 580
NmeAIII GCCGAG 1 cut(s) 265
PagI TCATGA 1 cut(s) 43
PfeI GAWTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 390
PkrI GCNGC 2 cut(s) 348, 445
PleI GAGTC 2 cut(s) 575, 642
PpsI GAGTC 2 cut(s) 575, 642
Psp124BI GAGCTC 1 cut(s) 254
PspPI GGNCC 1 cut(s) 531
PstI CTGCAG 1 cut(s) 325
PstNI CAGNNNCTG 1 cut(s) 260
RsaI GTAC 2 cut(s) 379, 404
RsaNI GTAC 2 cut(s) 378, 403
RseI CAYNNNNRTG 1 cut(s) 45
SacI GAGCTC 1 cut(s) 254
SaqAI TTAA 2 cut(s) 171, 368
SatI GCNGC 2 cut(s) 347, 444
Sau3AI GATC 3 cut(s) 37, 55, 326
Sau96I GGNCC 1 cut(s) 531
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 2 cut(s) 576, 643
SduI GDGCHC 2 cut(s) 254, 294
SetI ASST 8 cut(s) 84, 101, 197, 254, 488, 544, 559, 627
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 45
Sse9I AATT 3 cut(s) 116, 217, 476
SsiI CCGC 2 cut(s) 105, 573
SspMI CTAG 1 cut(s) 564
SstI GAGCTC 1 cut(s) 254
StyI CCWWGG 2 cut(s) 576, 582
TaiI ACGT 1 cut(s) 559
TaqI TCGA 3 cut(s) 13, 248, 271
TasI AATT 3 cut(s) 116, 217, 476
TatI WGTACW 2 cut(s) 377, 402
TfiI GAWTC 1 cut(s) 353
Tru1I TTAA 2 cut(s) 171, 368
Tru9I TTAA 2 cut(s) 171, 368
TscAI CASTG 1 cut(s) 457
TseI GCWGC 2 cut(s) 346, 443
TspDTI ATGAA 4 cut(s) 157, 279, 297, 501
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 1 cut(s) 457
Van91I CCANNNNNTGG 1 cut(s) 134
VneI GTGCAC 1 cut(s) 290
XapI RAATTY 1 cut(s) 476
XspI CTAG 1 cut(s) 564
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.