Rw5G050220
WRKY Family

WRKY DNA -binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018827
Physical Location & Seq
Reverse (-)
86796225 .. 86797345
1121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G050220.1

Sequence Viewer

Length: 489 bp
ATGAATTTCATAGTATTTAACATGCATATATATATGTCCGATTATCGATTGATTTTATATAGGGAAACTGATCATCATGATGAGGATGATCAGATATACGGAGAAGGAGAGGAGGTGCAAGACAACACCAGCTCAGGCGGAAGCAACAAATTGGTGATACCAGAAGATGGCTACGAATGGAAAAAATATGGACAAAAGTTCATTAAAAATGTTGGAAAGTTCAGGAGCTATTTCAAGTGCCAAAACAGCAATTGTAGAGCCAAGAAGCGAGCCGAGTGGTCGAGCTCAGAGCCTGATGAACTTCGAGTAGTGTATGAAGGGGTGCACAATCACACCGATAATAATGCCTCATCTGCAGGATCGGCTTCACATTCACAGCAGCCGGATTCCTCATCATCTTTAAATCCGAGTACTGATGCAAACCGGTATGACTTGTACACACAAGTTTTTGGTAATCAATCCTTCTCCAAGTGGGATCAAAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

162

Amino Acids

18.67

Weight (kDa)

5.47

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 55 - 111 1.3e-20 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018827)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45810
rosa_chinensis RchiOBHm_Chr5g0083891
rosa_laevigata RLG00000037062
rosa_multiflora Rmu_sc0001137.1_g000010
rosa_rugosa Rorug05G0487300 Rorug05G0487300
rosa_samantha Rh5CG587100 Rh5DG573700
rosa_wichuraiana Rw5G050220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 167
AciI CCGC 1 cut(s) 138
AclWI GGATC 2 cut(s) 367, 483
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 2 cut(s) 412, 437
AfiI CCNNNNNNNGG 1 cut(s) 167
AgeI ACCGGT 1 cut(s) 423
AgsI TTSAA 1 cut(s) 235
AluBI AGCT 3 cut(s) 132, 228, 285
AluI AGCT 3 cut(s) 132, 228, 285
Alw21I GWGCWC 2 cut(s) 287, 327
Alw44I GTGCAC 1 cut(s) 323
AlwI GGATC 2 cut(s) 367, 483
AlwNI CAGNNNCTG 1 cut(s) 293
ApaLI GTGCAC 1 cut(s) 323
ApeKI GCWGC 1 cut(s) 379
ApoI RAATTY 1 cut(s) 4
AsiGI ACCGGT 1 cut(s) 423
AsuHPI GGTGA 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 327
BanII GRGCYC 1 cut(s) 287
Bbv12I GWGCWC 2 cut(s) 287, 327
BbvI GCAGC 1 cut(s) 391
BccI CCATC 1 cut(s) 161
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BclI TGATCA 2 cut(s) 70, 88
BfmI CTRYAG 1 cut(s) 354
BisI GCNGC 1 cut(s) 380
BlsI GCNGC 1 cut(s) 381
BmcAI AGTACT 1 cut(s) 412
BmsI GCATC 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 423
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse118I RCCGGY 1 cut(s) 423
BseCI ATCGAT 1 cut(s) 46
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMII CTCAG 2 cut(s) 147, 300
BseRI GAGGAG 1 cut(s) 125
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 1 cut(s) 391
BshTI ACCGGT 1 cut(s) 423
BshVI ATCGAT 1 cut(s) 46
BsiHKAI GWGCWC 2 cut(s) 287, 327
BsiSI CCGG 2 cut(s) 383, 424
BslI CCNNNNNNNGG 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 287, 327
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 4 cut(s) 70, 88, 359, 475
BspACI CCGC 1 cut(s) 138
BspCNI CTCAG 2 cut(s) 146, 299
BspDI ATCGAT 1 cut(s) 46
BspHI TCATGA 1 cut(s) 76
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 2 cut(s) 367, 483
BsrFI RCCGGY 1 cut(s) 423
BsrGI TGTACA 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 423
BssMI GATC 4 cut(s) 70, 88, 359, 475
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 2 cut(s) 133, 286
BstF5I GGATG 1 cut(s) 91
BstKTI GATC 4 cut(s) 73, 91, 362, 478
BstMBI GATC 4 cut(s) 70, 88, 359, 475
BstMWI GCNNNNNNNGC 3 cut(s) 246, 353, 362
BstNSI RCATGY 1 cut(s) 25
BstSFI CTRYAG 1 cut(s) 354
BstSLI GKGCMC 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 391
Bsu15I ATCGAT 1 cut(s) 46
BsuTUI ATCGAT 1 cut(s) 46
BtsCI GGATG 1 cut(s) 91
Cac8I GCNNGC 1 cut(s) 270
CaiI CAGNNNCTG 1 cut(s) 293
CciI TCATGA 1 cut(s) 76
Cfr10I RCCGGY 1 cut(s) 423
ClaI ATCGAT 1 cut(s) 46
Csp6I GTAC 2 cut(s) 411, 436
CspAI ACCGGT 1 cut(s) 423
CviAII CATG 2 cut(s) 22, 77
CviJI RGCY 9 cut(s) 132, 171, 228, 260, 272, 285, 292, 365, 382
CviKI_1 RGCY 9 cut(s) 132, 171, 228, 260, 272, 285, 292, 365, 382
CviQI GTAC 2 cut(s) 411, 436
DdeI CTNAG 2 cut(s) 133, 286
DpnI GATC 4 cut(s) 72, 90, 361, 477
DpnII GATC 4 cut(s) 70, 88, 359, 475
DraI TTTAAA 1 cut(s) 402
EciI GGCGGA 1 cut(s) 153
Ecl136II GAGCTC 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 287
Eco53kI GAGCTC 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 285
EcoT22I ATGCAT 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 287
FaeI CATG 2 cut(s) 25, 80
FatI CATG 2 cut(s) 21, 76
FbaI TGATCA 2 cut(s) 70, 88
Fnu4HI GCNGC 1 cut(s) 380
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 1 cut(s) 380
GluI GCNGC 1 cut(s) 380
HapII CCGG 2 cut(s) 383, 424
Hin1II CATG 2 cut(s) 25, 80
HinfI GANTC 1 cut(s) 386
HpaII CCGG 2 cut(s) 383, 424
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 2 cut(s) 325, 438
Hpy188I TCNGA 4 cut(s) 40, 93, 289, 408
Hpy188III TCNNGA 2 cut(s) 77, 223
Hpy8I GTNNAC 2 cut(s) 325, 438
HpyAV CCTTC 3 cut(s) 98, 311, 472
HpyCH4V TGCA 5 cut(s) 25, 118, 325, 356, 419
HpyF10VI GCNNNNNNNGC 3 cut(s) 246, 353, 362
HpyF3I CTNAG 2 cut(s) 133, 286
Hsp92II CATG 2 cut(s) 25, 80
Ksp22I TGATCA 2 cut(s) 70, 88
Kzo9I GATC 4 cut(s) 70, 88, 359, 475
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 8 cut(s) 120, 142, 174, 208, 306, 342, 396, 437
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 1 cut(s) 406
MalI GATC 4 cut(s) 72, 90, 361, 477
MboI GATC 4 cut(s) 70, 88, 359, 475
MboII GAAGA 1 cut(s) 176
MfeI CAATTG 1 cut(s) 250
MhlI GDGCHC 2 cut(s) 287, 327
MluCI AATT 3 cut(s) 4, 149, 250
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 5 cut(s) 76, 103, 106, 358, 400
Mph1103I ATGCAT 1 cut(s) 27
MseI TTAA 3 cut(s) 18, 204, 401
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 2 cut(s) 383, 424
MunI CAATTG 1 cut(s) 250
MwoI GCNNNNNNNGC 3 cut(s) 246, 353, 362
NdeII GATC 4 cut(s) 70, 88, 359, 475
NlaIII CATG 2 cut(s) 25, 80
NmeAIII GCCGAG 1 cut(s) 298
NsiI ATGCAT 1 cut(s) 27
NspI RCATGY 1 cut(s) 25
PagI TCATGA 1 cut(s) 76
PfeI GAWTC 1 cut(s) 386
PflMI CCANNNNNTGG 1 cut(s) 167
PinAI ACCGGT 1 cut(s) 423
PkrI GCNGC 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 287
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 293
RsaI GTAC 2 cut(s) 412, 437
RsaNI GTAC 2 cut(s) 411, 436
RseI CAYNNNNRTG 1 cut(s) 78
SacI GAGCTC 1 cut(s) 287
SaqAI TTAA 3 cut(s) 18, 204, 401
SatI GCNGC 1 cut(s) 380
Sau3AI GATC 4 cut(s) 70, 88, 359, 475
ScaI AGTACT 1 cut(s) 412
SduI GDGCHC 2 cut(s) 287, 327
SetI ASST 4 cut(s) 117, 134, 230, 287
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 3 cut(s) 4, 149, 250
SsiI CCGC 1 cut(s) 138
SstI GAGCTC 1 cut(s) 287
TaqI TCGA 3 cut(s) 46, 281, 304
TasI AATT 3 cut(s) 4, 149, 250
TatI WGTACW 2 cut(s) 410, 435
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 3 cut(s) 18, 204, 401
Tru9I TTAA 3 cut(s) 18, 204, 401
TseI GCWGC 1 cut(s) 379
TspDTI ATGAA 4 cut(s) 17, 190, 312, 330
TspGWI ACGGA 1 cut(s) 114
Van91I CCANNNNNTGG 1 cut(s) 167
VneI GTGCAC 1 cut(s) 323
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 25
ZrmI AGTACT 1 cut(s) 412
Zsp2I ATGCAT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.