Rh5CG587100
WRKY Family

WRKY DNA -binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0018827
Physical Location & Seq
Reverse (-)
83218180 .. 83219936
1757 bp
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UTR
Exon/CDS
Intron
Rh5CG587100.1

Sequence Viewer

Length: 456 bp
ATGTCCGATTATCGATTGATTTTATATAGGGAAACTGATCATCATGATGAGGATGATCAGATATACGGAGAAGGAGAGGAGGTGCAAGACAACACCAGCTCAGGCGGAAGCAACAAATTGGTGATACCAGAAGATGGCTACGAATGGAAAAAATATGGACAAAAGTTCATTAAAAATGTTGGAAAGTTCAGGAGCTATTTCAAGTGCCAAAACAGCAATTGTAGAGCCAAGAAGCGAGCCGAGTGGTCGAGCTCAGAGCCTGATGAACTTCGAGTAGTGTATGAAGGGGTGCACAATCACACCGATAATAATGCCTCATCTGCAGGATCGGCTTCACATTCACAGCAGTCGGATTCCTCATCATCTTTAAATCCGAGTACTGATGCAAACCGGTATGACTTGTACACACAAGTTTTTGGTAATCAATCCTTCTCCAAGTGGGATCAAAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

151

Amino Acids

17.25

Weight (kDa)

5.33

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 44 - 100 1.1e-20 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018827)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45810
rosa_chinensis RchiOBHm_Chr5g0083891
rosa_laevigata RLG00000037062
rosa_multiflora Rmu_sc0001137.1_g000010
rosa_rugosa Rorug05G0487300 Rorug05G0487300
rosa_samantha Rh5CG587100 Rh5DG573700
rosa_wichuraiana Rw5G050220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 134
AciI CCGC 1 cut(s) 105
AclWI GGATC 2 cut(s) 334, 450
AfaI GTAC 2 cut(s) 379, 404
AfiI CCNNNNNNNGG 1 cut(s) 134
AgeI ACCGGT 1 cut(s) 390
AgsI TTSAA 1 cut(s) 202
AluBI AGCT 3 cut(s) 99, 195, 252
AluI AGCT 3 cut(s) 99, 195, 252
Alw21I GWGCWC 2 cut(s) 254, 294
Alw44I GTGCAC 1 cut(s) 290
AlwI GGATC 2 cut(s) 334, 450
AlwNI CAGNNNCTG 1 cut(s) 260
ApaLI GTGCAC 1 cut(s) 290
AsiGI ACCGGT 1 cut(s) 390
AsuHPI GGTGA 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 294
BanII GRGCYC 1 cut(s) 254
Bbv12I GWGCWC 2 cut(s) 254, 294
BccI CCATC 1 cut(s) 128
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BclI TGATCA 2 cut(s) 37, 55
BfmI CTRYAG 1 cut(s) 321
BmcAI AGTACT 1 cut(s) 379
BmsI GCATC 1 cut(s) 373
Bpu10I CCTNAGC 1 cut(s) 100
Bsa29I ATCGAT 1 cut(s) 13
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse118I RCCGGY 1 cut(s) 390
BseCI ATCGAT 1 cut(s) 13
BseGI GGATG 1 cut(s) 58
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 2 cut(s) 114, 267
BseRI GAGGAG 1 cut(s) 92
BseSI GKGCMC 1 cut(s) 294
BshTI ACCGGT 1 cut(s) 390
BshVI ATCGAT 1 cut(s) 13
BsiHKAI GWGCWC 2 cut(s) 254, 294
BsiSI CCGG 1 cut(s) 391
BslI CCNNNNNNNGG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 254, 294
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 4 cut(s) 37, 55, 326, 442
BspACI CCGC 1 cut(s) 105
BspCNI CTCAG 2 cut(s) 113, 266
BspDI ATCGAT 1 cut(s) 13
BspHI TCATGA 1 cut(s) 43
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 2 cut(s) 334, 450
BsrFI RCCGGY 1 cut(s) 390
BsrGI TGTACA 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 390
BssMI GATC 4 cut(s) 37, 55, 326, 442
BstAUI TGTACA 1 cut(s) 402
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 2 cut(s) 100, 253
BstF5I GGATG 1 cut(s) 58
BstKTI GATC 4 cut(s) 40, 58, 329, 445
BstMBI GATC 4 cut(s) 37, 55, 326, 442
BstMWI GCNNNNNNNGC 3 cut(s) 213, 320, 329
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 294
Bsu15I ATCGAT 1 cut(s) 13
BsuTUI ATCGAT 1 cut(s) 13
BtsCI GGATG 1 cut(s) 58
Cac8I GCNNGC 1 cut(s) 237
CaiI CAGNNNCTG 1 cut(s) 260
CciI TCATGA 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 390
ClaI ATCGAT 1 cut(s) 13
Csp6I GTAC 2 cut(s) 378, 403
CspAI ACCGGT 1 cut(s) 390
CviAII CATG 1 cut(s) 44
CviJI RGCY 8 cut(s) 99, 138, 195, 227, 239, 252, 259, 332
CviKI_1 RGCY 8 cut(s) 99, 138, 195, 227, 239, 252, 259, 332
CviQI GTAC 2 cut(s) 378, 403
DdeI CTNAG 2 cut(s) 100, 253
DpnI GATC 4 cut(s) 39, 57, 328, 444
DpnII GATC 4 cut(s) 37, 55, 326, 442
DraI TTTAAA 1 cut(s) 369
EciI GGCGGA 1 cut(s) 120
Ecl136II GAGCTC 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 254
Eco53kI GAGCTC 1 cut(s) 252
EcoICRI GAGCTC 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 254
FaeI CATG 1 cut(s) 47
FaiI YATR 7 cut(s) 25, 27, 45, 64, 156, 282, 396
FatI CATG 1 cut(s) 43
FbaI TGATCA 2 cut(s) 37, 55
FokI GGATG 1 cut(s) 65
FriOI GRGCYC 1 cut(s) 254
HapII CCGG 1 cut(s) 391
Hin1II CATG 1 cut(s) 47
HinfI GANTC 1 cut(s) 353
HpaII CCGG 1 cut(s) 391
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 2 cut(s) 292, 405
Hpy188I TCNGA 5 cut(s) 7, 60, 256, 352, 375
Hpy188III TCNNGA 2 cut(s) 44, 190
Hpy8I GTNNAC 2 cut(s) 292, 405
HpyAV CCTTC 3 cut(s) 65, 278, 439
HpyCH4V TGCA 4 cut(s) 85, 292, 323, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 213, 320, 329
HpyF3I CTNAG 2 cut(s) 100, 253
Hsp92II CATG 1 cut(s) 47
Ksp22I TGATCA 2 cut(s) 37, 55
Kzo9I GATC 4 cut(s) 37, 55, 326, 442
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 7 cut(s) 87, 109, 141, 175, 273, 309, 404
LweI GCATC 1 cut(s) 373
MalI GATC 4 cut(s) 39, 57, 328, 444
MboI GATC 4 cut(s) 37, 55, 326, 442
MboII GAAGA 1 cut(s) 143
MfeI CAATTG 1 cut(s) 217
MhlI GDGCHC 2 cut(s) 254, 294
MluCI AATT 2 cut(s) 116, 217
MmeI TCCRAC 2 cut(s) 160, 330
MnlI CCTC 5 cut(s) 43, 70, 73, 325, 367
MseI TTAA 2 cut(s) 171, 368
MslI CAYNNNNRTG 1 cut(s) 45
MspI CCGG 1 cut(s) 391
MunI CAATTG 1 cut(s) 217
MwoI GCNNNNNNNGC 3 cut(s) 213, 320, 329
NdeII GATC 4 cut(s) 37, 55, 326, 442
NlaIII CATG 1 cut(s) 47
NmeAIII GCCGAG 1 cut(s) 265
PagI TCATGA 1 cut(s) 43
PfeI GAWTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 254
PstI CTGCAG 1 cut(s) 325
PstNI CAGNNNCTG 1 cut(s) 260
RsaI GTAC 2 cut(s) 379, 404
RsaNI GTAC 2 cut(s) 378, 403
RseI CAYNNNNRTG 1 cut(s) 45
SacI GAGCTC 1 cut(s) 254
SaqAI TTAA 2 cut(s) 171, 368
Sau3AI GATC 4 cut(s) 37, 55, 326, 442
ScaI AGTACT 1 cut(s) 379
SduI GDGCHC 2 cut(s) 254, 294
SetI ASST 4 cut(s) 84, 101, 197, 254
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 45
Sse9I AATT 2 cut(s) 116, 217
SsiI CCGC 1 cut(s) 105
SstI GAGCTC 1 cut(s) 254
TaqI TCGA 3 cut(s) 13, 248, 271
TasI AATT 2 cut(s) 116, 217
TatI WGTACW 2 cut(s) 377, 402
TfiI GAWTC 1 cut(s) 353
Tru1I TTAA 2 cut(s) 171, 368
Tru9I TTAA 2 cut(s) 171, 368
TspDTI ATGAA 3 cut(s) 157, 279, 297
TspGWI ACGGA 1 cut(s) 81
Van91I CCANNNNNTGG 1 cut(s) 134
VneI GTGCAC 1 cut(s) 290
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.