RchiOBHm_Chr5g0083891
WRKY Family

WRKY DNA -binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018827
Physical Location & Seq
Reverse (-)
89560914 .. 89562040
1127 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35800

Sequence Viewer

Length: 423 bp
ATGTCGGAAACTGATCATCATGATGAGGATGATCAGATATACGGAGAAGGAGAGGAGGTGCAAGACAACACCAGCTCAGGCGGAAGCAACAAATTGGTGATACCAGAAGATGGCTACGAATGGAAAAAATATGGACAAAAGTTCATTAAAAATGTTGGAAAGTTCAGGAGCTATTTCAAGTGCCAAAACAGCAATTGTAGAGCCAAGAAGCGAGCCGAGTGGTCGAGCTCAGAGCCTGATGAACTTCGAGTAGTGTATGAAGGGGTGCACAATCACACCGATAATAATGCCTCATCTGCAGGATCGGCTTCACATTCACAGCAGCCGGATTCCTCATCATCTTTAAATCCGAGTACTGATGCAAACCGGTATGACTTGTACACACAAGTTTTTGGTAATCAACAAGGAGATAAAAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

140

Amino Acids

15.72

Weight (kDa)

5.16

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 36 - 92 9.5e-21 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018827)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45810
rosa_chinensis RchiOBHm_Chr5g0083891
rosa_laevigata RLG00000037062
rosa_multiflora Rmu_sc0001137.1_g000010
rosa_rugosa Rorug05G0487300 Rorug05G0487300
rosa_samantha Rh5CG587100 Rh5DG573700
rosa_wichuraiana Rw5G050220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 110
AciI CCGC 1 cut(s) 81
AclWI GGATC 1 cut(s) 310
AfaI GTAC 2 cut(s) 355, 380
AfiI CCNNNNNNNGG 1 cut(s) 110
AgeI ACCGGT 1 cut(s) 366
AgsI TTSAA 1 cut(s) 178
AluBI AGCT 3 cut(s) 75, 171, 228
AluI AGCT 3 cut(s) 75, 171, 228
Alw21I GWGCWC 2 cut(s) 230, 270
Alw44I GTGCAC 1 cut(s) 266
AlwI GGATC 1 cut(s) 310
AlwNI CAGNNNCTG 1 cut(s) 236
ApaLI GTGCAC 1 cut(s) 266
ApeKI GCWGC 1 cut(s) 322
AsiGI ACCGGT 1 cut(s) 366
AsuHPI GGTGA 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 230
Bbv12I GWGCWC 2 cut(s) 230, 270
BbvI GCAGC 1 cut(s) 334
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BclI TGATCA 2 cut(s) 13, 31
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 1 cut(s) 323
BlsI GCNGC 1 cut(s) 324
BmcAI AGTACT 1 cut(s) 355
BmsI GCATC 1 cut(s) 349
Bpu10I CCTNAGC 1 cut(s) 76
BsaWI WCCGGW 1 cut(s) 366
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 366
BseGI GGATG 1 cut(s) 34
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMII CTCAG 2 cut(s) 90, 243
BseRI GAGGAG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 270
BseXI GCAGC 1 cut(s) 334
BshTI ACCGGT 1 cut(s) 366
BsiHKAI GWGCWC 2 cut(s) 230, 270
BsiSI CCGG 2 cut(s) 326, 367
BslI CCNNNNNNNGG 1 cut(s) 110
Bsp1286I GDGCHC 2 cut(s) 230, 270
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 3 cut(s) 13, 31, 302
BspACI CCGC 1 cut(s) 81
BspCNI CTCAG 2 cut(s) 89, 242
BspHI TCATGA 1 cut(s) 19
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 1 cut(s) 310
BsrFI RCCGGY 1 cut(s) 366
BsrGI TGTACA 1 cut(s) 378
BssAI RCCGGY 1 cut(s) 366
BssMI GATC 3 cut(s) 13, 31, 302
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 2 cut(s) 76, 229
BstF5I GGATG 1 cut(s) 34
BstKTI GATC 3 cut(s) 16, 34, 305
BstMBI GATC 3 cut(s) 13, 31, 302
BstMWI GCNNNNNNNGC 3 cut(s) 189, 296, 305
BstSFI CTRYAG 1 cut(s) 297
BstSLI GKGCMC 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 334
BtsCI GGATG 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 213
CaiI CAGNNNCTG 1 cut(s) 236
CciI TCATGA 1 cut(s) 19
Cfr10I RCCGGY 1 cut(s) 366
Csp6I GTAC 2 cut(s) 354, 379
CspAI ACCGGT 1 cut(s) 366
CviAII CATG 1 cut(s) 20
CviJI RGCY 9 cut(s) 75, 114, 171, 203, 215, 228, 235, 308, 325
CviKI_1 RGCY 9 cut(s) 75, 114, 171, 203, 215, 228, 235, 308, 325
CviQI GTAC 2 cut(s) 354, 379
DdeI CTNAG 2 cut(s) 76, 229
DpnI GATC 3 cut(s) 15, 33, 304
DpnII GATC 3 cut(s) 13, 31, 302
DraI TTTAAA 1 cut(s) 345
EciI GGCGGA 1 cut(s) 96
Ecl136II GAGCTC 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 230
Eco53kI GAGCTC 1 cut(s) 228
EcoICRI GAGCTC 1 cut(s) 228
EcoT38I GRGCYC 1 cut(s) 230
FaeI CATG 1 cut(s) 23
FaiI YATR 5 cut(s) 21, 40, 132, 258, 372
FatI CATG 1 cut(s) 19
FbaI TGATCA 2 cut(s) 13, 31
Fnu4HI GCNGC 1 cut(s) 323
FokI GGATG 1 cut(s) 41
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 1 cut(s) 323
GluI GCNGC 1 cut(s) 323
HapII CCGG 2 cut(s) 326, 367
Hin1II CATG 1 cut(s) 23
HinfI GANTC 1 cut(s) 329
HpaII CCGG 2 cut(s) 326, 367
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 2 cut(s) 268, 381
Hpy188I TCNGA 4 cut(s) 7, 36, 232, 351
Hpy188III TCNNGA 2 cut(s) 20, 166
Hpy8I GTNNAC 2 cut(s) 268, 381
HpyAV CCTTC 2 cut(s) 41, 254
HpyCH4V TGCA 4 cut(s) 61, 268, 299, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 296, 305
HpyF3I CTNAG 2 cut(s) 76, 229
Hsp92II CATG 1 cut(s) 23
Ksp22I TGATCA 2 cut(s) 13, 31
Kzo9I GATC 3 cut(s) 13, 31, 302
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 8 cut(s) 63, 85, 117, 151, 249, 285, 339, 380
Lsp1109I GCAGC 1 cut(s) 334
LweI GCATC 1 cut(s) 349
MalI GATC 3 cut(s) 15, 33, 304
MboI GATC 3 cut(s) 13, 31, 302
MboII GAAGA 1 cut(s) 119
MfeI CAATTG 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 230, 270
MluCI AATT 2 cut(s) 92, 193
MmeI TCCRAC 1 cut(s) 136
MnlI CCTC 5 cut(s) 19, 46, 49, 301, 343
MseI TTAA 2 cut(s) 147, 344
MslI CAYNNNNRTG 1 cut(s) 21
MspI CCGG 2 cut(s) 326, 367
MunI CAATTG 1 cut(s) 193
MwoI GCNNNNNNNGC 3 cut(s) 189, 296, 305
NdeII GATC 3 cut(s) 13, 31, 302
NlaIII CATG 1 cut(s) 23
NmeAIII GCCGAG 1 cut(s) 241
PagI TCATGA 1 cut(s) 19
PfeI GAWTC 1 cut(s) 329
PflMI CCANNNNNTGG 1 cut(s) 110
PinAI ACCGGT 1 cut(s) 366
PkrI GCNGC 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 230
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 355, 380
RsaNI GTAC 2 cut(s) 354, 379
RseI CAYNNNNRTG 1 cut(s) 21
SacI GAGCTC 1 cut(s) 230
SaqAI TTAA 2 cut(s) 147, 344
SatI GCNGC 1 cut(s) 323
Sau3AI GATC 3 cut(s) 13, 31, 302
ScaI AGTACT 1 cut(s) 355
SduI GDGCHC 2 cut(s) 230, 270
SetI ASST 4 cut(s) 60, 77, 173, 230
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 21
Sse9I AATT 2 cut(s) 92, 193
SsiI CCGC 1 cut(s) 81
SstI GAGCTC 1 cut(s) 230
TaqI TCGA 2 cut(s) 224, 247
TasI AATT 2 cut(s) 92, 193
TatI WGTACW 2 cut(s) 353, 378
TfiI GAWTC 1 cut(s) 329
Tru1I TTAA 2 cut(s) 147, 344
Tru9I TTAA 2 cut(s) 147, 344
TseI GCWGC 1 cut(s) 322
TspDTI ATGAA 3 cut(s) 133, 255, 273
TspGWI ACGGA 1 cut(s) 57
Van91I CCANNNNNTGG 1 cut(s) 110
VneI GTGCAC 1 cut(s) 266
ZrmI AGTACT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.