Rh5DG573700
WRKY Family

WRKY DNA -binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018827
Physical Location & Seq
Reverse (-)
88076752 .. 88078349
1598 bp
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UTR
Exon/CDS
Intron
Rh5DG573700.1

Sequence Viewer

Length: 429 bp
ATGGAAACTGATCATCATGATGAGGATGATCAGATATACGGAGAAGGAGAGGAGGTGCAAGACAACACCAGCTCAGGCGGAAGCAACAAATTGGTGATACCAGAAGATGGCTACGAATGGAAAAAATATGGACAAAAGTTCATTAAAAATGTTGGAAAGTTCAGGAGCTATTTCAAGTGCCAAAACAGCAATTGTAGAGCCAAGAAGCGAGCCGAGTGGTCGAGCTCCGAGCCTGATGAACTTCGAATAGTGTATGAAGGGGTGCACAATCACACCGAAAATAATGCCTCATCTGCAGGATCGGCTTCACATTCACAGCAGCCGGATTCCTCATCATCTTTAAATCCGAGTACTGATGCAAACCGGTATGACTTGTACACACAAGTTTTTGGTAATCAATCCTTCTCCAAGTGGGATAAAAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

142

Amino Acids

16.11

Weight (kDa)

5.33

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 35 - 91 8.8e-21 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018827)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45810
rosa_chinensis RchiOBHm_Chr5g0083891
rosa_laevigata RLG00000037062
rosa_multiflora Rmu_sc0001137.1_g000010
rosa_rugosa Rorug05G0487300 Rorug05G0487300
rosa_samantha Rh5CG587100 Rh5DG573700
rosa_wichuraiana Rw5G050220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 107
AciI CCGC 1 cut(s) 78
AclWI GGATC 1 cut(s) 307
AfaI GTAC 2 cut(s) 352, 377
AfiI CCNNNNNNNGG 1 cut(s) 107
AgeI ACCGGT 1 cut(s) 363
AgsI TTSAA 1 cut(s) 175
AluBI AGCT 3 cut(s) 72, 168, 225
AluI AGCT 3 cut(s) 72, 168, 225
Alw21I GWGCWC 2 cut(s) 227, 267
Alw44I GTGCAC 1 cut(s) 263
AlwI GGATC 1 cut(s) 307
ApaLI GTGCAC 1 cut(s) 263
ApeKI GCWGC 1 cut(s) 319
AsiGI ACCGGT 1 cut(s) 363
AsuHPI GGTGA 1 cut(s) 106
AsuII TTCGAA 1 cut(s) 244
BaeGI GKGCMC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 227
Bbv12I GWGCWC 2 cut(s) 227, 267
BbvI GCAGC 1 cut(s) 331
BccI CCATC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BclI TGATCA 2 cut(s) 10, 28
BfmI CTRYAG 1 cut(s) 294
BisI GCNGC 1 cut(s) 320
BlsI GCNGC 1 cut(s) 321
BmcAI AGTACT 1 cut(s) 352
BmsI GCATC 1 cut(s) 346
Bpu10I CCTNAGC 1 cut(s) 73
Bpu14I TTCGAA 1 cut(s) 244
BsaWI WCCGGW 1 cut(s) 363
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse118I RCCGGY 1 cut(s) 363
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 65
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 1 cut(s) 331
BshTI ACCGGT 1 cut(s) 363
BsiHKAI GWGCWC 2 cut(s) 227, 267
BsiSI CCGG 2 cut(s) 323, 364
BslI CCNNNNNNNGG 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 244
Bsp1286I GDGCHC 2 cut(s) 227, 267
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 3 cut(s) 10, 28, 299
BspACI CCGC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 86
BspHI TCATGA 1 cut(s) 16
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 1 cut(s) 307
BspT104I TTCGAA 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 363
BsrGI TGTACA 1 cut(s) 375
BssAI RCCGGY 1 cut(s) 363
BssMI GATC 3 cut(s) 10, 28, 299
BstAUI TGTACA 1 cut(s) 375
BstBI TTCGAA 1 cut(s) 244
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 1 cut(s) 73
BstF5I GGATG 1 cut(s) 31
BstKTI GATC 3 cut(s) 13, 31, 302
BstMBI GATC 3 cut(s) 10, 28, 299
BstMWI GCNNNNNNNGC 3 cut(s) 186, 293, 302
BstSFI CTRYAG 1 cut(s) 294
BstSLI GKGCMC 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 331
BtsCI GGATG 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 210
CciI TCATGA 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 363
Csp6I GTAC 2 cut(s) 351, 376
CspAI ACCGGT 1 cut(s) 363
CviAII CATG 1 cut(s) 17
CviJI RGCY 9 cut(s) 72, 111, 168, 200, 212, 225, 232, 305, 322
CviKI_1 RGCY 9 cut(s) 72, 111, 168, 200, 212, 225, 232, 305, 322
CviQI GTAC 2 cut(s) 351, 376
DdeI CTNAG 1 cut(s) 73
DpnI GATC 3 cut(s) 12, 30, 301
DpnII GATC 3 cut(s) 10, 28, 299
DraI TTTAAA 1 cut(s) 342
EciI GGCGGA 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 227
Eco53kI GAGCTC 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 1 cut(s) 20
FaiI YATR 5 cut(s) 18, 37, 129, 255, 369
FatI CATG 1 cut(s) 16
FbaI TGATCA 2 cut(s) 10, 28
Fnu4HI GCNGC 1 cut(s) 320
FokI GGATG 1 cut(s) 38
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 1 cut(s) 320
GluI GCNGC 1 cut(s) 320
HapII CCGG 2 cut(s) 323, 364
Hin1II CATG 1 cut(s) 20
HinfI GANTC 1 cut(s) 326
HpaII CCGG 2 cut(s) 323, 364
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 265, 378
Hpy188I TCNGA 3 cut(s) 33, 229, 348
Hpy188III TCNNGA 2 cut(s) 17, 163
Hpy8I GTNNAC 2 cut(s) 265, 378
HpyAV CCTTC 3 cut(s) 38, 251, 412
HpyCH4V TGCA 4 cut(s) 58, 265, 296, 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 293, 302
HpyF3I CTNAG 1 cut(s) 73
Hsp92II CATG 1 cut(s) 20
Ksp22I TGATCA 2 cut(s) 10, 28
Kzo9I GATC 3 cut(s) 10, 28, 299
LmnI GCTCC 2 cut(s) 165, 230
LpnPI CCDG 8 cut(s) 60, 82, 114, 148, 246, 282, 336, 377
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 1 cut(s) 346
MalI GATC 3 cut(s) 12, 30, 301
MboI GATC 3 cut(s) 10, 28, 299
MboII GAAGA 1 cut(s) 116
MfeI CAATTG 1 cut(s) 190
MhlI GDGCHC 2 cut(s) 227, 267
MluCI AATT 2 cut(s) 89, 190
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 5 cut(s) 16, 43, 46, 298, 340
MseI TTAA 2 cut(s) 144, 341
MslI CAYNNNNRTG 1 cut(s) 18
MspI CCGG 2 cut(s) 323, 364
MunI CAATTG 1 cut(s) 190
MwoI GCNNNNNNNGC 3 cut(s) 186, 293, 302
NdeII GATC 3 cut(s) 10, 28, 299
NlaIII CATG 1 cut(s) 20
NmeAIII GCCGAG 1 cut(s) 238
NspV TTCGAA 1 cut(s) 244
PagI TCATGA 1 cut(s) 16
PfeI GAWTC 1 cut(s) 326
PflMI CCANNNNNTGG 1 cut(s) 107
PinAI ACCGGT 1 cut(s) 363
PkrI GCNGC 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 227
PstI CTGCAG 1 cut(s) 298
RsaI GTAC 2 cut(s) 352, 377
RsaNI GTAC 2 cut(s) 351, 376
RseI CAYNNNNRTG 1 cut(s) 18
SacI GAGCTC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 144, 341
SatI GCNGC 1 cut(s) 320
Sau3AI GATC 3 cut(s) 10, 28, 299
ScaI AGTACT 1 cut(s) 352
SduI GDGCHC 2 cut(s) 227, 267
SetI ASST 4 cut(s) 57, 74, 170, 227
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 244
SmiMI CAYNNNNRTG 1 cut(s) 18
Sse9I AATT 2 cut(s) 89, 190
SsiI CCGC 1 cut(s) 78
SstI GAGCTC 1 cut(s) 227
TaqI TCGA 2 cut(s) 221, 244
TasI AATT 2 cut(s) 89, 190
TatI WGTACW 2 cut(s) 350, 375
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 2 cut(s) 144, 341
Tru9I TTAA 2 cut(s) 144, 341
TseI GCWGC 1 cut(s) 319
TspDTI ATGAA 3 cut(s) 130, 252, 270
TspGWI ACGGA 1 cut(s) 54
Van91I CCANNNNNTGG 1 cut(s) 107
VneI GTGCAC 1 cut(s) 263
ZrmI AGTACT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.