Rorug05G0487300
WRKY Family

WRKY DNA -binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0018827
Physical Location & Seq
Forward (+)
67088659 .. 67091348
2690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0487300.1

Sequence Viewer

Length: 954 bp
ATGGAGTCTGGACATGAATTTGTTGATGTTCTCTTAAGCTACTTGACAATCCCCATAGGAACAATTATCAAGCTTGGCCGTAATAAGGATAATTCGGCACCACTGGGAATAGGTTGCATGAACAATTTGTATGCAAGTGTTGTGAATATGGATGTGCAGCATTTCAGGACTGAAGCTTGTAGGGACATGTTGCTATGTCCTCGAAATGGAGCTGGATCGACTTTAAAGAGCCTCAAACTCAAAATTGATGACCGTGATCCTACACAGTACTTTAAGTGCTCGAATGTGTCTTGCCGCATATCTCCGGCTAGGCTAAGCTATTACAGAAGTGTTCGCTGTTGTCGTTGTGGAAATCAAATGGACTCAAAGAGCAACCTTTTAGTTGGCTGTCTTGATGGGGTCTTTGTAAAAGGACTAGCCAGACTTATAATTAGTGACGATTTACAAGTCATGCCTGCAGTAACATCAGTAAGCTCTTCTTTGTTGATGAAGCCTGGGGTCATGGATTCAAATACTGTTGAGGAGGTGACTTTGGACATTGGAGTTGATGAGGTTGTCAATTTGCTTATGTGCTCATTTATATCAAAATCACCTCTGACTGATGTTCTTCTACAGCATAGACCATTAGCAAACTTGAATAAAAATATCAGTAACCAACCAATATGTGTAGATTACTATTCTGCAAGAGTAGAAGACACAGTGAATGATGAAGCGAACATTACTTTGAAGCTTATACTAAGCAAATCGAAGAAGGTGATTTGCTATGCAGAAGCAGGGGAAGATTTTGTTAATTTGCTTTTCAGCTTCCTAGTTCTTCCACTTGGATTTTTGTTAAAACAAATGTGGCTTGGTACTTTGAAGGGATGCATTGATCACCTGTACAAAAGTGTCCGGGATCTTGATCAGCAGCTCTTGAAGTCTAGATACTCTAAGGAAGTACTAGTTAATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

317

Amino Acids

35.25

Weight (kDa)

7.89

Isoelectric Point (pI)

28.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 2 - 313 4.6e-97 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018827)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45810
rosa_chinensis RchiOBHm_Chr5g0083891
rosa_laevigata RLG00000037062
rosa_multiflora Rmu_sc0001137.1_g000010
rosa_rugosa Rorug05G0487300 Rorug05G0487300
rosa_samantha Rh5CG587100 Rh5DG573700
rosa_wichuraiana Rw5G050220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 428
AccB1I GGYRCC 1 cut(s) 97
AciI CCGC 1 cut(s) 295
AclWI GGATC 3 cut(s) 223, 251, 903
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 1 cut(s) 17
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 4 cut(s) 269, 853, 881, 939
AfiI CCNNNNNNNGG 2 cut(s) 85, 206
AflII CTTAAG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 186
AgsI TTSAA 5 cut(s) 510, 637, 727, 859, 916
AhlI ACTAGT 1 cut(s) 940
AjnI CCWGG 1 cut(s) 493
AluBI AGCT 9 cut(s) 39, 73, 176, 212, 318, 474, 730, 804, 910
AluI AGCT 9 cut(s) 39, 73, 176, 212, 318, 474, 730, 804, 910
Alw21I GWGCWC 2 cut(s) 281, 575
AlwI GGATC 3 cut(s) 223, 251, 903
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 2 cut(s) 157, 907
ApoI RAATTY 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 893
AsuHPI GGTGA 4 cut(s) 538, 582, 766, 866
BanI GGYRCC 1 cut(s) 97
BarI GAAGNNNNNNTAC 2 cut(s) 908, 940
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 2 cut(s) 281, 575
BbvI GCAGC 2 cut(s) 169, 919
BccI CCATC 1 cut(s) 389
BceAI ACGGC 1 cut(s) 63
BciT130I CCWGG 1 cut(s) 495
BclI TGATCA 2 cut(s) 871, 901
BcnI CCSGG 1 cut(s) 893
BcuI ACTAGT 1 cut(s) 940
BfaI CTAG 6 cut(s) 309, 416, 809, 921, 941, 952
BfmI CTRYAG 2 cut(s) 456, 611
BfrI CTTAAG 1 cut(s) 34
BisI GCNGC 3 cut(s) 158, 295, 908
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 3 cut(s) 159, 296, 909
BmcAI AGTACT 2 cut(s) 269, 939
Bme1390I CCNGG 2 cut(s) 495, 893
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 2 cut(s) 495, 893
BmrI ACTGGG 1 cut(s) 113
BmsI GCATC 1 cut(s) 854
BmuI ACTGGG 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 893
BsaBI GATNNNNATC 1 cut(s) 900
BsaJI CCNNGG 1 cut(s) 494
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 206
Bse1I ACTGG 1 cut(s) 108
Bse8I GATNNNNATC 1 cut(s) 900
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 1 cut(s) 494
BseGI GGATG 2 cut(s) 157, 869
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 2 cut(s) 85, 206
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 2 cut(s) 169, 919
BsgI GTGCAG 1 cut(s) 176
BshFI GGCC 1 cut(s) 78
BshNI GGYRCC 1 cut(s) 97
BsiHKAI GWGCWC 2 cut(s) 281, 575
BsiSI CCGG 2 cut(s) 305, 892
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 2 cut(s) 85, 206
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 281, 575
Bsp1407I TGTACA 1 cut(s) 879
Bsp143I GATC 5 cut(s) 215, 256, 871, 895, 901
Bsp1720I GCTNAGC 1 cut(s) 314
BspACI CCGC 1 cut(s) 295
BspANI GGCC 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 99
BspMAI CTGCAG 1 cut(s) 460
BspPI GGATC 3 cut(s) 223, 251, 903
BspQI GCTCTTC 1 cut(s) 481
BspT107I GGYRCC 1 cut(s) 97
BspTI CTTAAG 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 879
BsrI ACTGG 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 494
BssMI GATC 5 cut(s) 215, 256, 871, 895, 901
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 4 cut(s) 254, 267, 517, 700
Bst6I CTCTTC 1 cut(s) 481
BstAFI CTTAAG 1 cut(s) 34
BstAUI TGTACA 1 cut(s) 879
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 3 cut(s) 314, 737, 930
BstF5I GGATG 2 cut(s) 157, 869
BstKTI GATC 5 cut(s) 218, 259, 874, 898, 904
BstMBI GATC 5 cut(s) 215, 256, 871, 895, 901
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 1 cut(s) 190
BstSCI CCNGG 2 cut(s) 493, 891
BstSFI CTRYAG 2 cut(s) 456, 611
BstV1I GCAGC 2 cut(s) 169, 919
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 1 cut(s) 895
BstYI RGATCY 1 cut(s) 895
BsuRI GGCC 1 cut(s) 78
BtsCI GGATG 2 cut(s) 157, 869
BtsIMutI CAGTG 2 cut(s) 101, 705
Cac8I GCNNGC 1 cut(s) 456
Csp6I GTAC 4 cut(s) 268, 852, 880, 938
CviAII CATG 5 cut(s) 14, 118, 187, 451, 502
CviQI GTAC 4 cut(s) 268, 852, 880, 938
DdeI CTNAG 3 cut(s) 314, 737, 930
DpnI GATC 5 cut(s) 217, 258, 873, 897, 903
DpnII GATC 5 cut(s) 215, 256, 871, 895, 901
DraI TTTAAA 1 cut(s) 225
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 481
EarI CTCTTC 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 493
EcoT22I ATGCAT 1 cut(s) 869
FaeI CATG 5 cut(s) 17, 121, 190, 454, 505
FalI AAGNNNNNCTT 2 cut(s) 463, 495
FaqI GGGAC 1 cut(s) 197
FatI CATG 5 cut(s) 13, 117, 186, 450, 501
FbaI TGATCA 2 cut(s) 871, 901
Fnu4HI GCNGC 3 cut(s) 158, 295, 908
FokI GGATG 2 cut(s) 164, 876
Fsp4HI GCNGC 3 cut(s) 158, 295, 908
FspBI CTAG 6 cut(s) 309, 416, 809, 921, 941, 952
GluI GCNGC 3 cut(s) 158, 295, 908
HaeIII GGCC 1 cut(s) 78
HapII CCGG 2 cut(s) 305, 892
Hin1II CATG 5 cut(s) 17, 121, 190, 454, 505
HindIII AAGCTT 3 cut(s) 71, 174, 728
HinfI GANTC 3 cut(s) 5, 362, 506
HpaII CCGG 2 cut(s) 305, 892
HphI GGTGA 4 cut(s) 538, 582, 766, 866
Hpy188I TCNGA 1 cut(s) 597
Hpy188III TCNNGA 6 cut(s) 9, 166, 392, 899, 913, 921
HpyAV CCTTC 2 cut(s) 745, 853
HpyCH4III ACNGT 4 cut(s) 254, 267, 517, 700
HpyCH4V TGCA 7 cut(s) 117, 134, 157, 458, 683, 767, 867
HpyF3I CTNAG 3 cut(s) 314, 737, 930
Hsp92II CATG 5 cut(s) 17, 121, 190, 454, 505
Ksp22I TGATCA 2 cut(s) 871, 901
Kzo9I GATC 5 cut(s) 215, 256, 871, 895, 901
LguI GCTCTTC 1 cut(s) 481
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 2 cut(s) 169, 919
LweI GCATC 1 cut(s) 854
MaeI CTAG 6 cut(s) 309, 416, 809, 921, 941, 952
MaeIII GTNAC 4 cut(s) 434, 460, 526, 650
MalI GATC 5 cut(s) 217, 258, 873, 897, 903
MboI GATC 5 cut(s) 215, 256, 871, 895, 901
MboII GAAGA 6 cut(s) 468, 599, 704, 760, 791, 806
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 2 cut(s) 281, 575
MluCI AATT 8 cut(s) 17, 63, 91, 124, 243, 429, 559, 790
MlyI GAGTC 2 cut(s) 14, 356
MnlI CCTC 6 cut(s) 210, 242, 514, 517, 544, 603
Mph1103I ATGCAT 1 cut(s) 869
MseI TTAA 6 cut(s) 35, 224, 273, 789, 833, 945
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 2 cut(s) 305, 892
MspR9I CCNGG 2 cut(s) 495, 893
MvaI CCWGG 1 cut(s) 495
NciI CCSGG 1 cut(s) 893
NdeII GATC 5 cut(s) 215, 256, 871, 895, 901
NlaIII CATG 5 cut(s) 17, 121, 190, 454, 505
NlaIV GGNNCC 1 cut(s) 99
NmuCI GTSAC 2 cut(s) 434, 526
NsiI ATGCAT 1 cut(s) 869
NspI RCATGY 1 cut(s) 190
PciI ACATGT 1 cut(s) 186
PciSI GCTCTTC 1 cut(s) 481
PcsI WCGNNNNNNNCGW 1 cut(s) 340
PfeI GAWTC 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 891
PkrI GCNGC 3 cut(s) 159, 296, 909
PleI GAGTC 2 cut(s) 13, 356
PpsI GAGTC 2 cut(s) 13, 356
PscI ACATGT 1 cut(s) 186
PsiI TTATAA 1 cut(s) 428
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 1 cut(s) 99
PstI CTGCAG 1 cut(s) 460
PsuI RGATCY 1 cut(s) 895
RsaI GTAC 4 cut(s) 269, 853, 881, 939
RsaNI GTAC 4 cut(s) 268, 852, 880, 938
SapI GCTCTTC 1 cut(s) 481
SaqAI TTAA 6 cut(s) 35, 224, 273, 789, 833, 945
SatI GCNGC 3 cut(s) 158, 295, 908
Sau3AI GATC 5 cut(s) 215, 256, 871, 895, 901
ScaI AGTACT 2 cut(s) 269, 939
SchI GAGTC 2 cut(s) 14, 356
ScrFI CCNGG 2 cut(s) 495, 893
SduI GDGCHC 2 cut(s) 281, 575
SfaNI GCATC 1 cut(s) 854
SfcI CTRYAG 2 cut(s) 456, 611
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
SpeI ACTAGT 1 cut(s) 940
Sse9I AATT 8 cut(s) 17, 63, 91, 124, 243, 429, 559, 790
SsiI CCGC 1 cut(s) 295
SspMI CTAG 6 cut(s) 309, 416, 809, 921, 941, 952
StyD4I CCNGG 2 cut(s) 493, 891
TaaI ACNGT 4 cut(s) 254, 267, 517, 700
TaqI TCGA 4 cut(s) 202, 218, 281, 746
TasI AATT 8 cut(s) 17, 63, 91, 124, 243, 429, 559, 790
TatI WGTACW 3 cut(s) 267, 879, 937
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 6 cut(s) 35, 224, 273, 789, 833, 945
Tru9I TTAA 6 cut(s) 35, 224, 273, 789, 833, 945
TscAI CASTG 2 cut(s) 108, 705
TseFI GTSAC 2 cut(s) 434, 526
TseI GCWGC 2 cut(s) 157, 907
Tsp45I GTSAC 2 cut(s) 434, 526
TspDTI ATGAA 4 cut(s) 30, 134, 503, 723
TspRI CASTG 2 cut(s) 108, 705
Vha464I CTTAAG 1 cut(s) 34
XapI RAATTY 1 cut(s) 17
XbaI TCTAGA 1 cut(s) 920
XceI RCATGY 1 cut(s) 190
XspI CTAG 6 cut(s) 309, 416, 809, 921, 941, 952
ZrmI AGTACT 2 cut(s) 269, 939
Zsp2I ATGCAT 1 cut(s) 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.