RLG00000037062
WRKY Family

WRKY DNA -binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018827
Physical Location & Seq
Reverse (-)
87981810 .. 87982691
882 bp
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UTR
Exon/CDS
Intron
RLM00000037062

Sequence Viewer

Length: 471 bp
ATGGCTTTGTTAAGTAAGTACTCAAAAATATCTTTAGATTCTATGGAAGCTGATCATCATGATGAGGATGATCAGACATACGGAGAAGGAGAGGAGGTGCAAGACAACACCAACTCAGGCGGAAGCAACAAATTGGTGATACCAGAAGATGGCTACGAATGGAAAAAATATGGACAAAAGTTCATTAAAAATGTTAGAAAGTTCAGGAGCTATTTCAAGTGCCAAAACAGCAATTGTAGAGCCAAGAAGCGAGCCGAGTGGTCAAGCTCAGAGCCTGATGAACTTCGAGTAGTGTATGAAGGGGTGCACAATCACACCGATAATAATGCCTCATCTGCAGGATCGGCTTCACATTCACAGCAGCCGGATTCCTCATCATCTTTAAATCCGAGTACTGATGCAAACCGCATCTTACCCCTTAATAAATTGGACAAGCCTCGCCATTTCAATGAGTTGCATGCCAGTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

157

Amino Acids

17.62

Weight (kDa)

5.96

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 49 - 105 7.3e-21 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018827)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45810
rosa_chinensis RchiOBHm_Chr5g0083891
rosa_laevigata RLG00000037062
rosa_multiflora Rmu_sc0001137.1_g000010
rosa_rugosa Rorug05G0487300 Rorug05G0487300
rosa_samantha Rh5CG587100 Rh5DG573700
rosa_wichuraiana Rw5G050220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 149
AciI CCGC 2 cut(s) 120, 406
AclWI GGATC 1 cut(s) 349
AfaI GTAC 2 cut(s) 20, 394
AfiI CCNNNNNNNGG 1 cut(s) 149
AgsI TTSAA 2 cut(s) 217, 448
AluBI AGCT 3 cut(s) 50, 210, 267
AluI AGCT 3 cut(s) 50, 210, 267
Alw21I GWGCWC 1 cut(s) 309
Alw44I GTGCAC 1 cut(s) 305
AlwI GGATC 1 cut(s) 349
AlwNI CAGNNNCTG 1 cut(s) 275
ApaLI GTGCAC 1 cut(s) 305
ApeKI GCWGC 1 cut(s) 361
AsuHPI GGTGA 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 1 cut(s) 373
BccI CCATC 1 cut(s) 143
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BclI TGATCA 2 cut(s) 52, 70
BfmI CTRYAG 1 cut(s) 336
BisI GCNGC 1 cut(s) 362
BlsI GCNGC 1 cut(s) 363
BmcAI AGTACT 2 cut(s) 20, 394
BmsI GCATC 2 cut(s) 388, 417
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 462
BseGI GGATG 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMII CTCAG 2 cut(s) 129, 282
BseNI ACTGG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 1 cut(s) 365
BslI CCNNNNNNNGG 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 3 cut(s) 52, 70, 341
BspACI CCGC 2 cut(s) 120, 406
BspCNI CTCAG 2 cut(s) 128, 281
BspHI TCATGA 1 cut(s) 58
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 1 cut(s) 349
BsrI ACTGG 1 cut(s) 462
BssMI GATC 3 cut(s) 52, 70, 341
BstC8I GCNNGC 2 cut(s) 252, 459
BstDEI CTNAG 2 cut(s) 115, 268
BstF5I GGATG 1 cut(s) 73
BstKTI GATC 3 cut(s) 55, 73, 344
BstMBI GATC 3 cut(s) 52, 70, 341
BstMWI GCNNNNNNNGC 3 cut(s) 228, 335, 344
BstNSI RCATGY 1 cut(s) 461
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 373
BtsCI GGATG 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 252, 459
CaiI CAGNNNCTG 1 cut(s) 275
CciI TCATGA 1 cut(s) 58
Csp6I GTAC 2 cut(s) 19, 393
CviAII CATG 2 cut(s) 59, 458
CviQI GTAC 2 cut(s) 19, 393
DdeI CTNAG 2 cut(s) 115, 268
DpnI GATC 3 cut(s) 54, 72, 343
DpnII GATC 3 cut(s) 52, 70, 341
DraI TTTAAA 1 cut(s) 384
EciI GGCGGA 1 cut(s) 135
FaeI CATG 2 cut(s) 62, 461
FaiI YATR 6 cut(s) 44, 60, 79, 171, 297, 459
FatI CATG 2 cut(s) 58, 457
FbaI TGATCA 2 cut(s) 52, 70
Fnu4HI GCNGC 1 cut(s) 362
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 1 cut(s) 362
GluI GCNGC 1 cut(s) 362
HapII CCGG 1 cut(s) 365
Hin1II CATG 2 cut(s) 62, 461
HinfI GANTC 2 cut(s) 38, 368
HpaII CCGG 1 cut(s) 365
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 3 cut(s) 75, 271, 390
Hpy188III TCNNGA 2 cut(s) 59, 205
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 2 cut(s) 80, 293
HpyCH4V TGCA 5 cut(s) 100, 307, 338, 401, 457
HpyF10VI GCNNNNNNNGC 3 cut(s) 228, 335, 344
HpyF3I CTNAG 2 cut(s) 115, 268
Hsp92II CATG 2 cut(s) 62, 461
Ksp22I TGATCA 2 cut(s) 52, 70
Kzo9I GATC 3 cut(s) 52, 70, 341
LmnI GCTCC 1 cut(s) 207
LpnPI CCDG 6 cut(s) 102, 156, 190, 288, 324, 378
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 2 cut(s) 388, 417
MalI GATC 3 cut(s) 54, 72, 343
MboI GATC 3 cut(s) 52, 70, 341
MboII GAAGA 1 cut(s) 158
MfeI CAATTG 1 cut(s) 232
MhlI GDGCHC 1 cut(s) 309
MluCI AATT 3 cut(s) 131, 232, 425
MnlI CCTC 6 cut(s) 58, 85, 88, 340, 382, 447
MseI TTAA 4 cut(s) 11, 186, 383, 420
MslI CAYNNNNRTG 2 cut(s) 60, 447
MspI CCGG 1 cut(s) 365
MunI CAATTG 1 cut(s) 232
MwoI GCNNNNNNNGC 3 cut(s) 228, 335, 344
NdeII GATC 3 cut(s) 52, 70, 341
NlaIII CATG 2 cut(s) 62, 461
NmeAIII GCCGAG 1 cut(s) 280
NspI RCATGY 1 cut(s) 461
PaeI GCATGC 1 cut(s) 461
PagI TCATGA 1 cut(s) 58
PfeI GAWTC 2 cut(s) 38, 368
PflMI CCANNNNNTGG 1 cut(s) 149
PkrI GCNGC 1 cut(s) 363
PstI CTGCAG 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 275
RsaI GTAC 2 cut(s) 20, 394
RsaNI GTAC 2 cut(s) 19, 393
RseI CAYNNNNRTG 2 cut(s) 60, 447
SaqAI TTAA 4 cut(s) 11, 186, 383, 420
SatI GCNGC 1 cut(s) 362
Sau3AI GATC 3 cut(s) 52, 70, 341
ScaI AGTACT 2 cut(s) 20, 394
SduI GDGCHC 1 cut(s) 309
SetI ASST 4 cut(s) 52, 99, 212, 269
SfaNI GCATC 2 cut(s) 388, 417
SfcI CTRYAG 1 cut(s) 336
SmiMI CAYNNNNRTG 2 cut(s) 60, 447
SphI GCATGC 1 cut(s) 461
Sse9I AATT 3 cut(s) 131, 232, 425
SsiI CCGC 2 cut(s) 120, 406
TaqI TCGA 1 cut(s) 286
TasI AATT 3 cut(s) 131, 232, 425
TatI WGTACW 2 cut(s) 18, 392
TfiI GAWTC 2 cut(s) 38, 368
Tru1I TTAA 4 cut(s) 11, 186, 383, 420
Tru9I TTAA 4 cut(s) 11, 186, 383, 420
TseI GCWGC 1 cut(s) 361
TspDTI ATGAA 3 cut(s) 172, 294, 312
TspGWI ACGGA 1 cut(s) 96
Van91I CCANNNNNTGG 1 cut(s) 149
VneI GTGCAC 1 cut(s) 305
XceI RCATGY 1 cut(s) 461
ZrmI AGTACT 2 cut(s) 20, 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.