FvH4_4g00550

Coiled-coil regions of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005999
Physical Location & Seq
Forward (+)
484016 .. 491828
7813 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00550.t5

Sequence Viewer

Length: 1476 bp
ATGACGAAGATAAGTCCTGAATTTGAAGACAATATCGCAACAGACGGAGTTCTTTCAGTTTCAGCTGATGTCAGCTTCATTTCTAATGGTTTCCCAAAGTACAAACTAGGACCTGATAATCAGATACTGGACGAGCCAAAGGGAGGTAGCGATGGTCCAAACTTAAAAGAGGTAGTTGAAGAAGAAACTACCCAACTGTCAGAGCAGCATAAACGTCTTTCAGTTCGTGACCTTGCCAGTAAATTTGATAAGAACTTGGCTGCGGCTGCTAAATTGTCTGAAGAGGCAAAAATTAGAGAAGTGGCTTCATTGGAGGGACATGTGCTTTTGAAGAAACTTAGAGATGCATTGGAATATTTGAGAGGGCTTTTAGCAGGAAGAAACAAGGAGGATGTGGAGGAAGCTATTTCTATGGTGGAAGCTTTGGCCGTTAAGTTGACTCAAAAAGAAGGAGAACTGATTCAGGACAAGTTTGAAGTGAAAAAGCTAGCCAGCTATCTCAAACAGACTTCTGAAGATGCAAAAAAACTGGTTAACCAAGAGAAATCTTTTGCCTGTGCTGAAATCGAAAGTGCAAGGGGAATAGTCAAGAGAATTGGAGAGGCTCTTGAAGAAAGAAACAGAACCACAGAAGATCCTAAAAGACAGGATGTGGAGGAACTAGTTGAGGTGGTCCAGGAAGCTAGGAGAATTAAATTGATGCATCAGCCAAGCAAGGTGATGGATATGGAACATGAGCTTCGTGCATTACGTACACAGATTCGAGAGAAATGCCTAGTTTCAGTGAAGCTTCAGAAAGAGCTGGCAATGAGCAAGAGGGAAGCAGAAAACAAATCTCGTGGATATGCTATGCTTGGTTCTGAAACTCTGGGTTCATATCTTCATATCCAAACTTGCTCAGAGAATGTTCCACAGCTTCCAAGCTGTTCAATCCAGTGGTATCGTGTTTCACCAGACGGTAGCTGGAATGAAGCTATTTCAGGTGCCAATAAATCAATTTATGCTCCAGAGCCTTTTGATGTTGGAAGAACCTTAGGTGCAGACATCATTTCCAATGGTCAGAAAAAGACTGTTACGACTGCTGGTCCAATTGATCCTGCTGCAGGACTTCAAAGCCACATAGACACACTTTTGCGAAAATCTAACACAGAATTTCATGTTGTCATTTCACAAATGAATGGGCGTGATCATCCATCACGTTCTGTTCACATATTTCATGTGGGAAAGATTAGGATGAAGCTGTGTAGAGGATGGATTACAAAGTCCAGAGAAATTTATTCCTCATCGATGCAGCTATGTGGTGCTAGAGGTGATCTTGCTAATGTGGCCAAGGGGGTGTTTTGGCAACCAAGGAAAGGTCTTTCATTTGTATTAACTTTTGAAACAGAGCGAGATAGAAACGCAGCATTACTTCTTGCCAGGAAATATGCTCTTGATTGCAATGTTATGCTTGCTGGACCAGATGATAGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007623 GO:0008150 GO:0016020 GO:0044464 GO:0048511 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAB-ABD SCAB-IgPH SCAB_CC

Protein Analysis

492

Amino Acids

54.79

Weight (kDa)

7.6

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 55 - 97 6e-25 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 101 - 268 1.8e-72 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 282 - 379 3.3e-42 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 382 - 431 1.4e-20 PH domain of plant-specific actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 983
AciI CCGC 1 cut(s) 263
AclWI GGATC 2 cut(s) 629, 1088
AcoI YGGCCR 2 cut(s) 426, 1326
AcsI RAATTY 4 cut(s) 20, 242, 1151, 1272
AcuI CTGAAG 3 cut(s) 300, 534, 776
AfaI GTAC 2 cut(s) 101, 754
AfiI CCNNNNNNNGG 3 cut(s) 143, 1103, 1355
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 8 cut(s) 26, 179, 331, 476, 611, 930, 1112, 1382
AhdI GACNNNNNGTC 1 cut(s) 1083
AhlI ACTAGT 1 cut(s) 661
AjnI CCWGG 2 cut(s) 675, 1418
AlwI GGATC 2 cut(s) 629, 1088
AlwNI CAGNNNCTG 1 cut(s) 127
AoxI GGCC 2 cut(s) 426, 1326
ApeKI GCWGC 6 cut(s) 205, 260, 266, 1100, 1291, 1403
ApoI RAATTY 4 cut(s) 20, 242, 1151, 1272
Asp700I GAANNNNTTC 1 cut(s) 459
AspS9I GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
AsuHPI GGTGA 3 cut(s) 730, 942, 1322
AsuNHI GCTAGC 1 cut(s) 487
AvaII GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
AxyI CCTNAGG 1 cut(s) 1033
BalI TGGCCA 1 cut(s) 1328
BanI GGYRCC 1 cut(s) 983
BauI CACGAG 1 cut(s) 837
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 6 cut(s) 217, 247, 253, 1087, 1303, 1415
BccI CCATC 4 cut(s) 146, 715, 1201, 1245
BceAI ACGGC 1 cut(s) 413
BcgI CGANNNNNNTGC 2 cut(s) 753, 787
BciT130I CCWGG 2 cut(s) 677, 1420
BclI TGATCA 1 cut(s) 1186
BcuI ACTAGT 1 cut(s) 661
BfaI CTAG 6 cut(s) 107, 488, 662, 684, 776, 1305
BfmI CTRYAG 1 cut(s) 1101
BisI GCNGC 7 cut(s) 206, 261, 264, 267, 1101, 1292, 1404
BlsI GCNGC 7 cut(s) 207, 262, 265, 268, 1102, 1293, 1405
Bme1390I CCNGG 2 cut(s) 677, 1420
Bme18I GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
BmeRI GACNNNNNGTC 1 cut(s) 1083
BmgT120I GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
BmiI GGNNCC 1 cut(s) 985
BmrFI CCNGG 2 cut(s) 677, 1420
BmsI GCATC 5 cut(s) 334, 508, 690, 712, 1278
BmtI GCTAGC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 990
Bsa29I ATCGAT 1 cut(s) 1286
BsaAI YACGTR 1 cut(s) 752
BsaJI CCNNGG 2 cut(s) 1329, 1349
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 1103, 1355
Bse1I ACTGG 4 cut(s) 132, 237, 534, 934
Bse21I CCTNAGG 1 cut(s) 1033
Bse3DI GCAATG 2 cut(s) 813, 1447
BseBI CCWGG 2 cut(s) 677, 1420
BseCI ATCGAT 1 cut(s) 1286
BseDI CCNNGG 2 cut(s) 1329, 1349
BseGI GGATG 5 cut(s) 397, 655, 1189, 1239, 1256
BseLI CCNNNNNNNGG 3 cut(s) 143, 1103, 1355
BseMI GCAATG 2 cut(s) 813, 1447
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 4 cut(s) 132, 237, 534, 934
BseXI GCAGC 6 cut(s) 217, 247, 253, 1087, 1303, 1415
BsgI GTGCAG 1 cut(s) 1059
BshFI GGCC 2 cut(s) 428, 1328
BshNI GGYRCC 1 cut(s) 983
BshVI ATCGAT 1 cut(s) 1286
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 3 cut(s) 143, 1103, 1355
BsmFI GGGAC 1 cut(s) 330
BsnI GGCC 2 cut(s) 428, 1328
Bsp143I GATC 4 cut(s) 634, 1093, 1186, 1312
BspACI CCGC 1 cut(s) 263
BspANI GGCC 2 cut(s) 428, 1328
BspCNI CTCAG 1 cut(s) 911
BspDI ATCGAT 1 cut(s) 1286
BspLI GGNNCC 1 cut(s) 985
BspMAI CTGCAG 1 cut(s) 1105
BspOI GCTAGC 1 cut(s) 491
BspPI GGATC 2 cut(s) 629, 1088
BspT107I GGYRCC 1 cut(s) 983
BsrDI GCAATG 2 cut(s) 813, 1447
BsrI ACTGG 4 cut(s) 132, 237, 534, 934
BssECI CCNNGG 2 cut(s) 1329, 1349
BssMI GATC 4 cut(s) 634, 1093, 1186, 1312
BssSI CACGAG 1 cut(s) 837
BssT1I CCWWGG 2 cut(s) 1329, 1349
Bst2BI CACGAG 1 cut(s) 837
Bst2UI CCWGG 2 cut(s) 677, 1420
Bst4CI ACNGT 3 cut(s) 198, 959, 1072
Bst6I CTCTTC 1 cut(s) 276
BstBAI YACGTR 1 cut(s) 752
BstC8I GCNNGC 4 cut(s) 489, 493, 804, 1452
BstDEI CTNAG 3 cut(s) 338, 898, 1033
BstENI CCTNNNNNAGG 1 cut(s) 1101
BstF5I GGATG 5 cut(s) 397, 655, 1189, 1239, 1256
BstKTI GATC 4 cut(s) 637, 1096, 1189, 1315
BstMBI GATC 4 cut(s) 634, 1093, 1186, 1312
BstMWI GCNNNNNNNGC 2 cut(s) 266, 1325
BstNI CCWGG 2 cut(s) 677, 1420
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 2 cut(s) 675, 1418
BstSFI CTRYAG 1 cut(s) 1101
BstSNI TACGTA 1 cut(s) 752
BstV1I GCAGC 6 cut(s) 217, 247, 253, 1087, 1303, 1415
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
Bsu15I ATCGAT 1 cut(s) 1286
Bsu36I CCTNAGG 1 cut(s) 1033
BsuRI GGCC 2 cut(s) 428, 1328
BsuTUI ATCGAT 1 cut(s) 1286
BtgZI GCGATG 1 cut(s) 165
BtsCI GGATG 5 cut(s) 397, 655, 1189, 1239, 1256
BtsIMutI CAGTG 2 cut(s) 789, 941
Cac8I GCNNGC 4 cut(s) 489, 493, 804, 1452
CaiI CAGNNNCTG 1 cut(s) 127
Cfr13I GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
ClaI ATCGAT 1 cut(s) 1286
Csp6I GTAC 2 cut(s) 100, 753
CspCI CAANNNNNGTGG 2 cut(s) 820, 855
CviAII CATG 4 cut(s) 320, 734, 1157, 1217
CviQI GTAC 2 cut(s) 100, 753
DdeI CTNAG 3 cut(s) 338, 898, 1033
DpnI GATC 4 cut(s) 636, 1095, 1188, 1314
DpnII GATC 4 cut(s) 634, 1093, 1186, 1312
DriI GACNNNNNGTC 1 cut(s) 1083
EaeI YGGCCR 2 cut(s) 426, 1326
Eam1104I CTCTTC 1 cut(s) 276
Eam1105I GACNNNNNGTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 276
Eco105I TACGTA 1 cut(s) 752
Eco130I CCWWGG 2 cut(s) 1329, 1349
Eco47I GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
Eco57I CTGAAG 3 cut(s) 300, 534, 776
Eco81I CCTNAGG 1 cut(s) 1033
EcoNI CCTNNNNNAGG 1 cut(s) 1101
EcoO109I RGGNCCY 1 cut(s) 110
EcoRII CCWGG 2 cut(s) 675, 1418
EcoT14I CCWWGG 2 cut(s) 1329, 1349
EcoT22I ATGCAT 2 cut(s) 349, 705
ErhI CCWWGG 2 cut(s) 1329, 1349
FaeI CATG 4 cut(s) 323, 737, 1160, 1220
FaqI GGGAC 1 cut(s) 330
FatI CATG 4 cut(s) 319, 733, 1156, 1216
FbaI TGATCA 1 cut(s) 1186
Fnu4HI GCNGC 7 cut(s) 206, 261, 264, 267, 1101, 1292, 1404
FokI GGATG 5 cut(s) 404, 662, 1176, 1246, 1263
Fsp4HI GCNGC 7 cut(s) 206, 261, 264, 267, 1101, 1292, 1404
FspBI CTAG 6 cut(s) 107, 488, 662, 684, 776, 1305
GluI GCNGC 7 cut(s) 206, 261, 264, 267, 1101, 1292, 1404
GsuI CTGGAG 1 cut(s) 990
HaeIII GGCC 2 cut(s) 428, 1328
Hin1II CATG 4 cut(s) 323, 737, 1160, 1220
HincII GTYRAC 2 cut(s) 438, 535
HindII GTYRAC 2 cut(s) 438, 535
HindIII AAGCTT 2 cut(s) 420, 788
HinfI GANTC 3 cut(s) 439, 460, 760
HpaI GTTAAC 1 cut(s) 535
HphI GGTGA 3 cut(s) 730, 942, 1322
Hpy166II GTNNAC 4 cut(s) 438, 535, 755, 1207
Hpy188I TCNGA 8 cut(s) 123, 202, 280, 514, 795, 862, 901, 1062
Hpy188III TCNNGA 9 cut(s) 17, 227, 464, 589, 608, 764, 1007, 1266, 1433
Hpy8I GTNNAC 4 cut(s) 438, 535, 755, 1207
HpyAV CCTTC 1 cut(s) 443
HpyCH4III ACNGT 3 cut(s) 198, 959, 1072
HpyCH4IV ACGT 3 cut(s) 214, 751, 1198
HpyCH4V TGCA 9 cut(s) 347, 521, 575, 703, 746, 1040, 1103, 1291, 1440
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 1325
HpyF3I CTNAG 3 cut(s) 338, 898, 1033
HpySE526I ACGT 3 cut(s) 214, 751, 1198
Hsp92II CATG 4 cut(s) 323, 737, 1160, 1220
Ksp22I TGATCA 1 cut(s) 1186
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 4 cut(s) 634, 1093, 1186, 1312
LmnI GCTCC 1 cut(s) 1009
Lsp1109I GCAGC 6 cut(s) 217, 247, 253, 1087, 1303, 1415
LweI GCATC 5 cut(s) 334, 508, 690, 712, 1278
MaeI CTAG 6 cut(s) 107, 488, 662, 684, 776, 1305
MaeII ACGT 3 cut(s) 214, 751, 1198
MaeIII GTNAC 2 cut(s) 227, 1072
MalI GATC 4 cut(s) 636, 1095, 1188, 1314
MboI GATC 4 cut(s) 634, 1093, 1186, 1312
MfeI CAATTG 1 cut(s) 1089
MflI RGATCY 1 cut(s) 634
MlsI TGGCCA 1 cut(s) 1328
MluNI TGGCCA 1 cut(s) 1328
MlyI GAGTC 1 cut(s) 433
MmeI TCCRAC 1 cut(s) 1003
Mox20I TGGCCA 1 cut(s) 1328
Mph1103I ATGCAT 2 cut(s) 349, 705
MroXI GAANNNNTTC 1 cut(s) 459
MscI TGGCCA 1 cut(s) 1328
MseI TTAA 5 cut(s) 164, 432, 534, 693, 1373
Msp20I TGGCCA 1 cut(s) 1328
MspA1I CMGCKG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 677, 1420
MunI CAATTG 1 cut(s) 1089
MvaI CCWGG 2 cut(s) 677, 1420
MwoI GCNNNNNNNGC 2 cut(s) 266, 1325
NdeII GATC 4 cut(s) 634, 1093, 1186, 1312
NheI GCTAGC 1 cut(s) 487
NlaIII CATG 4 cut(s) 323, 737, 1160, 1220
NlaIV GGNNCC 1 cut(s) 985
NmuCI GTSAC 1 cut(s) 227
NsiI ATGCAT 2 cut(s) 349, 705
NspI RCATGY 1 cut(s) 323
PciI ACATGT 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 748
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 460, 760
PfoI TCCNGGA 1 cut(s) 675
PkrI GCNGC 7 cut(s) 207, 262, 265, 268, 1102, 1293, 1405
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
Ppu21I YACGTR 1 cut(s) 752
PpuMI RGGWCCY 1 cut(s) 110
PscI ACATGT 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 675, 1418
PspGI CCWGG 2 cut(s) 675, 1418
PspN4I GGNNCC 1 cut(s) 985
PspPI GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
PspPPI RGGWCCY 1 cut(s) 110
PstI CTGCAG 1 cut(s) 1105
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 634
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 2 cut(s) 101, 754
RsaNI GTAC 2 cut(s) 100, 753
SaqAI TTAA 5 cut(s) 164, 432, 534, 693, 1373
SatI GCNGC 7 cut(s) 206, 261, 264, 267, 1101, 1292, 1404
Sau3AI GATC 4 cut(s) 634, 1093, 1186, 1312
Sau96I GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 2 cut(s) 677, 1420
SfaNI GCATC 5 cut(s) 334, 508, 690, 712, 1278
SfcI CTRYAG 1 cut(s) 1101
SinI GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
SnaBI TACGTA 1 cut(s) 752
SpeI ACTAGT 1 cut(s) 661
SsiI CCGC 1 cut(s) 263
SspI AATATT 1 cut(s) 356
SspMI CTAG 6 cut(s) 107, 488, 662, 684, 776, 1305
StyD4I CCNGG 2 cut(s) 675, 1418
StyI CCWWGG 2 cut(s) 1329, 1349
TaaI ACNGT 3 cut(s) 198, 959, 1072
TaiI ACGT 3 cut(s) 217, 754, 1201
TaqI TCGA 3 cut(s) 567, 763, 1286
TatI WGTACW 1 cut(s) 99
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 2 cut(s) 460, 760
Tru1I TTAA 5 cut(s) 164, 432, 534, 693, 1373
Tru9I TTAA 5 cut(s) 164, 432, 534, 693, 1373
TscAI CASTG 2 cut(s) 789, 941
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 6 cut(s) 205, 260, 266, 1100, 1291, 1403
Tsp45I GTSAC 1 cut(s) 227
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 2 cut(s) 789, 941
VpaK11BI GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
XagI CCTNNNNNAGG 1 cut(s) 1101
XapI RAATTY 4 cut(s) 20, 242, 1151, 1272
XceI RCATGY 1 cut(s) 323
XcmI CCANNNNNNNNNTGG 1 cut(s) 960
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 6 cut(s) 107, 488, 662, 684, 776, 1305
Zsp2I ATGCAT 2 cut(s) 349, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.