Rroxscaffold_5G00333770

Coiled-coil regions of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005999
Physical Location & Seq
Forward (+)
1137264 .. 1150395
13132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00333770.1

Sequence Viewer

Length: 1950 bp
ATGACCCAACTGTCAGAGCAGCATAAACGTCTTTCAGTTCGTGACCTTGCCAGTAAATTTGACAAGAACTTGGCTGCTGCTGCTAAATTGTCTGAAGAGGCAAAAATTAGAGAAGTGGCTTCTTTGGAGGGACATGTTCTTTTGAAGAAGTTGAGAGATGCATTGGAATATTTGAGAGGGCTTTTAGCAGGTAGAAACAAGGAGGATGTGGAGGAAGCTATTTCTATGGTGGAGGCTTTAGCTGTTAAGTTGACTCAAAAGGAAGGAGAATTGATTCAGGACAAGTTTGAAGTGAAAAAGCTAGCCAGCTTTCTCAAACAGACTTCTGAAGATGCTAAAAAGCTGGTTAGCCAAGAGAAATCTTTTGCCTGTGCTGAAATCGAAAGTGCGAGGGGAATAGTCAAGAGAATTGGAGAGGCTCTTGAAGAACGAAACAGAACTACAGAAGATCCTAAAAAACAGGATGTGGAGGAACTAGTTGAGGTGGTCCAGGAAGCTAGGAGAATTAAATTGATGCATCAGCCAAGCAAGGTGATGGACATGGAACATGAGCTTCGTGCATTACGAACACAGATTCGAGAGAAATGCATAGTTTCAGTGAAGCTTCAGAAAGAGCTGGCAATGAGCAAGAGGGCAGAGGAGAACAAATCTCGCGGATATGCTATAGTTGGTTCTGAAACTCTAGGTTCATATCTACAAATCCAAACTTGCTCAGAAAATGCTCCACAGCTTTCAAACTGTTCAATCCAGTGGTATCGTGTTTCACCAGATGGCAGCTGGAATGAAGCTATTTCAGGTGCCAATAAATTAACTTATGCTCCAGAGCCTTTTGATGTTGGAAGAACCTTGGGTGCAGACATTATTTCCAATGGTCGAAAAGTGACAGTTACAACTGCTGGTCCAATTGATCCTGCTGCAGGACTTGAAAGCTACATAGACACACTTTTGCGAAAATCTAACACGGAATTTCATCTATGTGGCGCTAGAGGTGATGTTGCTAATGTGGCCAAGGGAGTGTTTTGGCAACCAAGGAAGGGTCTTTCATTTGTATTGACATTTGAAACAGAGCGAGATAGAAACGCAGCAATACTTCTTGCCAGGAAATATGCTCTTGATTGCAATGCTATTGCGCAGCTCATTCCAAGCCATGGTCGGTTGGCAAAGGAATTGTATACAATCCCTAACTCGACATTTTGGGAAAGCAAGACTAGTCGAGGCCACTTCAACTCCTCAGACCCAACAATCACTCTTGCGGCGCCCTTTTTATCACTACTCTCAACACTGGATCTTTCATCGGCTAAATGGCTTCTCAATAACTTCTATGTATGGGGTTATTCTCAACTTCCCCCTTTTTCTCAGGACCCTAACAAGATCGACCTGGTTGGCAAACTGGTTCGTGGTATGCGTGTTGAAGATGTGTTGTCACAATTGTCAGTGACCGACAAGCAAGCTGCAAAAACCATGTATCAGGCTATTTACTCAGCTCGAGCAGATGCAACTTATAAACATGGGTTGGATCCAGATCGTCTACTTGTTGCTGGGGCATCTATTGGAAAGGGATTCGTGGGATTCAACAAGAAGAGACTGGCCTACCATGGTAAAGGCAATGGAATTAAAGTGAGACCAAAACACCAACTGATGGTGGTTTTAAGGGAGATTGCCCCTGAAGAGGAGGCGGAGATAGCCAGACAAAGAGCCGACAATTTTCTTTATCTCAGTAAGCGCCTCAGGCTCACTAAGGAGGAAAATAGGAATATTCCTCTCCAGCTTATTCGGAAAAACAAAGGCAAATTCAGTAATGGTGAACCAAGTGGAGGTACTAGGAAGGACCACAAGGAGGCACGCAATGGCCTGTTTTACCCGAAGTCCGACACCGCGCCGCGCCAAAGACGCGACACCCCCCTCCGGCATCGAGTACACCAAATAGGTGGGCTTGCCCTCCCCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007623 GO:0008150 GO:0016020 GO:0044464 GO:0048511 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAB-ABD SCAB-IgPH SCAB_CC

Protein Analysis

649

Amino Acids

72.63

Weight (kDa)

9.48

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 2 - 35 3.9e-19 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 39 - 206 2.2e-72 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 220 - 317 1.4e-43 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 321 - 376 1e-22 PH domain of plant-specific actin-binding protein
Ribosomal_L22 PF00237 454 - 540 2.2e-13 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1503
AasI GACNNNNNNGTC 1 cut(s) 10
Acc16I TGCGCA 1 cut(s) 1131
Acc36I ACCTGC 1 cut(s) 179
AccB1I GGYRCC 2 cut(s) 797, 1255
AccB7I CCANNNNNTGG 2 cut(s) 1148, 1639
AccI GTMKAC 2 cut(s) 1172, 1528
AccII CGCG 4 cut(s) 654, 1877, 1882, 1893
AciI CCGC 6 cut(s) 654, 1253, 1676, 1875, 1880, 1944
AclWI GGATC 5 cut(s) 443, 902, 1293, 1511, 1524
AcoI YGGCCR 1 cut(s) 1005
AcsI RAATTY 3 cut(s) 56, 965, 1790
AcuI CTGAAG 4 cut(s) 114, 348, 590, 1686
AcyI GRCGYC 1 cut(s) 1256
AfaI GTAC 2 cut(s) 1819, 1917
AfiI CCNNNNNNNGG 8 cut(s) 917, 1034, 1148, 1639, 1669, 1814, 1837, 1905
AflIII ACRYGT 1 cut(s) 133
AhlI ACTAGT 2 cut(s) 475, 1208
AjnI CCWGG 3 cut(s) 489, 1097, 1377
Alw26I GTCTC 2 cut(s) 1576, 1615
AlwI GGATC 5 cut(s) 443, 902, 1293, 1511, 1524
Ama87I CYCGRG 1 cut(s) 1485
AoxI GGCC 4 cut(s) 1005, 1216, 1587, 1849
ApeKI GCWGC 9 cut(s) 19, 74, 77, 80, 774, 914, 1082, 1132, 1451
ApoI RAATTY 3 cut(s) 56, 965, 1790
Asp700I GAANNNNTTC 1 cut(s) 273
AspLEI GCGC 6 cut(s) 983, 1132, 1258, 1725, 1879, 1884
AspS9I GGNCC 4 cut(s) 487, 899, 1360, 1828
AsuHPI GGTGA 4 cut(s) 544, 756, 1001, 1814
AsuNHI GCTAGC 1 cut(s) 301
AvaI CYCGRG 1 cut(s) 1485
AvaII GGWCC 4 cut(s) 487, 899, 1360, 1828
AxyI CCTNAGG 1 cut(s) 1727
BalI TGGCCA 1 cut(s) 1007
BamHI GGATCC 1 cut(s) 1516
BanI GGYRCC 2 cut(s) 797, 1255
BbvI GCAGC 9 cut(s) 31, 61, 64, 67, 786, 901, 1094, 1144, 1438
BccI CCATC 3 cut(s) 529, 764, 1633
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciT130I CCWGG 3 cut(s) 491, 1099, 1379
BcoDI GTCTC 2 cut(s) 1576, 1615
BcuI ACTAGT 2 cut(s) 475, 1208
BfaI CTAG 7 cut(s) 302, 476, 498, 683, 984, 1209, 1821
BfmI CTRYAG 3 cut(s) 441, 663, 915
BfoI RGCGCY 3 cut(s) 984, 1259, 1726
BfuAI ACCTGC 1 cut(s) 179
Bme1390I CCNGG 3 cut(s) 491, 1099, 1379
Bme18I GGWCC 4 cut(s) 487, 899, 1360, 1828
BmeT110I CYCGRG 1 cut(s) 1485
BmgT120I GGNCC 4 cut(s) 487, 899, 1360, 1828
BmiI GGNNCC 4 cut(s) 799, 1257, 1362, 1518
BmrFI CCNGG 3 cut(s) 491, 1099, 1379
BmsI GCATC 7 cut(s) 148, 322, 504, 526, 1483, 1553, 1918
BmtI GCTAGC 1 cut(s) 305
BpmI CTGGAG 2 cut(s) 804, 1748
BsaBI GATNNNNATC 1 cut(s) 1521
BsaHI GRCGYC 1 cut(s) 1256
BsaI GGTCTC 1 cut(s) 1615
BsaJI CCNNGG 5 cut(s) 846, 1008, 1028, 1147, 1594
BsaXI ACNNNNNCTCC 4 cut(s) 802, 832, 1211, 1241
Bsc4I CCNNNNNNNGG 8 cut(s) 917, 1034, 1148, 1639, 1669, 1814, 1837, 1905
Bse1I ACTGG 5 cut(s) 51, 748, 1287, 1395, 1590
Bse21I CCTNAGG 1 cut(s) 1727
Bse3DI GCAATG 4 cut(s) 627, 1126, 1612, 1852
Bse8I GATNNNNATC 1 cut(s) 1521
BseBI CCWGG 3 cut(s) 491, 1099, 1379
BseDI CCNNGG 5 cut(s) 846, 1008, 1028, 1147, 1594
BseGI GGATG 2 cut(s) 211, 469
BseJI GATNNNNATC 1 cut(s) 1521
BseLI CCNNNNNNNGG 8 cut(s) 917, 1034, 1148, 1639, 1669, 1814, 1837, 1905
BseMI GCAATG 4 cut(s) 627, 1126, 1612, 1852
BseMII CTCAG 6 cut(s) 726, 1245, 1370, 1494, 1729, 1741
BseNI ACTGG 5 cut(s) 51, 748, 1287, 1395, 1590
BseRI GAGGAG 3 cut(s) 653, 1219, 1685
BseXI GCAGC 9 cut(s) 31, 61, 64, 67, 786, 901, 1094, 1144, 1438
BseYI CCCAGC 1 cut(s) 1538
BsgI GTGCAG 1 cut(s) 873
Bsh1236I CGCG 4 cut(s) 654, 1877, 1882, 1893
BshFI GGCC 4 cut(s) 1007, 1218, 1589, 1851
BshNI GGYRCC 2 cut(s) 797, 1255
BsiHKCI CYCGRG 1 cut(s) 1485
BsiSI CCGG 1 cut(s) 1906
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 8 cut(s) 917, 1034, 1148, 1639, 1669, 1814, 1837, 1905
BsmAI GTCTC 2 cut(s) 1576, 1615
BsmFI GGGAC 1 cut(s) 144
BsnI GGCC 4 cut(s) 1007, 1218, 1589, 1851
Bso31I GGTCTC 1 cut(s) 1615
BsoBI CYCGRG 1 cut(s) 1485
Bsp143I GATC 6 cut(s) 448, 907, 1285, 1371, 1516, 1522
Bsp19I CCATGG 2 cut(s) 1147, 1594
BspACI CCGC 6 cut(s) 654, 1253, 1676, 1875, 1880, 1944
BspANI GGCC 4 cut(s) 1007, 1218, 1589, 1851
BspCNI CTCAG 6 cut(s) 725, 1244, 1369, 1493, 1728, 1740
BspFNI CGCG 4 cut(s) 654, 1877, 1882, 1893
BspLI GGNNCC 4 cut(s) 799, 1257, 1362, 1518
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 179
BspOI GCTAGC 1 cut(s) 305
BspPI GGATC 5 cut(s) 443, 902, 1293, 1511, 1524
BspT107I GGYRCC 2 cut(s) 797, 1255
BspTNI GGTCTC 1 cut(s) 1615
BsrDI GCAATG 4 cut(s) 627, 1126, 1612, 1852
BsrI ACTGG 5 cut(s) 51, 748, 1287, 1395, 1590
BssECI CCNNGG 5 cut(s) 846, 1008, 1028, 1147, 1594
BssMI GATC 6 cut(s) 448, 907, 1285, 1371, 1516, 1522
BssNAI GTATAC 1 cut(s) 1173
BssNI GRCGYC 1 cut(s) 1256
BssT1I CCWWGG 5 cut(s) 846, 1008, 1028, 1147, 1594
Bst1107I GTATAC 1 cut(s) 1173
Bst2UI CCWGG 3 cut(s) 491, 1099, 1379
Bst4CI ACNGT 3 cut(s) 12, 740, 886
Bst6I CTCTTC 3 cut(s) 90, 1574, 1662
BstACI GRCGYC 1 cut(s) 1256
BstC8I GCNNGC 6 cut(s) 303, 307, 618, 1449, 1843, 1935
BstDEI CTNAG 7 cut(s) 712, 1231, 1356, 1480, 1715, 1727, 1737
BstDSI CCRYGG 2 cut(s) 1147, 1594
BstENI CCTNNNNNAGG 1 cut(s) 915
BstF5I GGATG 2 cut(s) 211, 469
BstFNI CGCG 4 cut(s) 654, 1877, 1882, 1893
BstH2I RGCGCY 3 cut(s) 984, 1259, 1726
BstHHI GCGC 6 cut(s) 983, 1132, 1258, 1725, 1879, 1884
BstKTI GATC 6 cut(s) 451, 910, 1288, 1374, 1519, 1525
BstMAI GTCTC 2 cut(s) 1576, 1615
BstMBI GATC 6 cut(s) 448, 907, 1285, 1371, 1516, 1522
BstMWI GCNNNNNNNGC 6 cut(s) 80, 1004, 1682, 1729, 1890, 1943
BstNI CCWGG 3 cut(s) 491, 1099, 1379
BstNSI RCATGY 1 cut(s) 137
BstSCI CCNGG 3 cut(s) 489, 1097, 1377
BstSFI CTRYAG 3 cut(s) 441, 663, 915
BstUI CGCG 4 cut(s) 654, 1877, 1882, 1893
BstV1I GCAGC 9 cut(s) 31, 61, 64, 67, 786, 901, 1094, 1144, 1438
BstX2I RGATCY 3 cut(s) 448, 1285, 1516
BstXI CCANNNNNNTGG 1 cut(s) 1928
BstYI RGATCY 3 cut(s) 448, 1285, 1516
BstZ17I GTATAC 1 cut(s) 1173
Bsu36I CCTNAGG 1 cut(s) 1727
BsuRI GGCC 4 cut(s) 1007, 1218, 1589, 1851
BtgI CCRYGG 2 cut(s) 1147, 1594
BtsCI GGATG 2 cut(s) 211, 469
BtsIMutI CAGTG 4 cut(s) 603, 755, 1280, 1440
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 6 cut(s) 303, 307, 618, 1449, 1843, 1935
CfoI GCGC 6 cut(s) 983, 1132, 1258, 1725, 1879, 1884
Cfr13I GGNCC 4 cut(s) 487, 899, 1360, 1828
CseI GACGC 1 cut(s) 1899
CsiI ACCWGGT 1 cut(s) 1377
Csp6I GTAC 2 cut(s) 1818, 1916
CviAII CATG 7 cut(s) 134, 541, 548, 1148, 1462, 1508, 1595
CviQI GTAC 2 cut(s) 1818, 1916
DdeI CTNAG 7 cut(s) 712, 1231, 1356, 1480, 1715, 1727, 1737
DinI GGCGCC 1 cut(s) 1257
DpnI GATC 6 cut(s) 450, 909, 1287, 1373, 1518, 1524
DpnII GATC 6 cut(s) 448, 907, 1285, 1371, 1516, 1522
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 1005
Eam1104I CTCTTC 3 cut(s) 90, 1574, 1662
EarI CTCTTC 3 cut(s) 90, 1574, 1662
EciI GGCGGA 1 cut(s) 1691
Eco130I CCWWGG 5 cut(s) 846, 1008, 1028, 1147, 1594
Eco31I GGTCTC 1 cut(s) 1615
Eco47I GGWCC 4 cut(s) 487, 899, 1360, 1828
Eco57I CTGAAG 4 cut(s) 114, 348, 590, 1686
Eco81I CCTNAGG 1 cut(s) 1727
Eco88I CYCGRG 1 cut(s) 1485
EcoNI CCTNNNNNAGG 1 cut(s) 915
EcoO109I RGGNCCY 1 cut(s) 1360
EcoRII CCWGG 3 cut(s) 489, 1097, 1377
EcoT14I CCWWGG 5 cut(s) 846, 1008, 1028, 1147, 1594
EcoT22I ATGCAT 3 cut(s) 163, 519, 590
EgeI GGCGCC 1 cut(s) 1257
EheI GGCGCC 1 cut(s) 1257
ErhI CCWWGG 5 cut(s) 846, 1008, 1028, 1147, 1594
FaeI CATG 7 cut(s) 137, 544, 551, 1151, 1465, 1511, 1598
FaqI GGGAC 1 cut(s) 144
FatI CATG 7 cut(s) 133, 540, 547, 1147, 1461, 1507, 1594
FblI GTMKAC 2 cut(s) 1172, 1528
FokI GGATG 2 cut(s) 218, 476
FspBI CTAG 7 cut(s) 302, 476, 498, 683, 984, 1209, 1821
FspI TGCGCA 1 cut(s) 1131
GlaI GCGC 6 cut(s) 982, 1131, 1257, 1724, 1878, 1883
GsaI CCCAGC 1 cut(s) 1542
GsuI CTGGAG 2 cut(s) 804, 1748
HaeII RGCGCY 3 cut(s) 984, 1259, 1726
HaeIII GGCC 4 cut(s) 1007, 1218, 1589, 1851
HapII CCGG 1 cut(s) 1906
HgaI GACGC 1 cut(s) 1899
HhaI GCGC 6 cut(s) 983, 1132, 1258, 1725, 1879, 1884
Hin1I GRCGYC 1 cut(s) 1256
Hin1II CATG 7 cut(s) 137, 544, 551, 1151, 1465, 1511, 1598
Hin6I GCGC 6 cut(s) 981, 1130, 1256, 1723, 1877, 1882
HinP1I GCGC 6 cut(s) 981, 1130, 1256, 1723, 1877, 1882
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 5 cut(s) 253, 274, 574, 1560, 1569
HpaII CCGG 1 cut(s) 1906
HphI GGTGA 4 cut(s) 544, 756, 1001, 1814
Hpy166II GTNNAC 5 cut(s) 252, 1173, 1529, 1805, 1918
Hpy188I TCNGA 9 cut(s) 16, 94, 328, 609, 676, 715, 1234, 1776, 1870
Hpy188III TCNNGA 9 cut(s) 41, 278, 403, 422, 578, 821, 1112, 1358, 1520
Hpy8I GTNNAC 5 cut(s) 252, 1173, 1529, 1805, 1918
HpyAV CCTTC 3 cut(s) 257, 1027, 1819
HpyCH4III ACNGT 3 cut(s) 12, 740, 886
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 9 cut(s) 161, 517, 560, 588, 854, 917, 1119, 1454, 1496
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 1004, 1682, 1729, 1890, 1943
HpyF3I CTNAG 7 cut(s) 712, 1231, 1356, 1480, 1715, 1727, 1737
HpySE526I ACGT 1 cut(s) 28
Hsp92I GRCGYC 1 cut(s) 1256
Hsp92II CATG 7 cut(s) 137, 544, 551, 1151, 1465, 1511, 1598
HspAI GCGC 6 cut(s) 981, 1130, 1256, 1723, 1877, 1882
KasI GGCGCC 1 cut(s) 1255
Kzo9I GATC 6 cut(s) 448, 907, 1285, 1371, 1516, 1522
LmnI GCTCC 2 cut(s) 727, 823
Lsp1109I GCAGC 9 cut(s) 31, 61, 64, 67, 786, 901, 1094, 1144, 1438
LweI GCATC 7 cut(s) 148, 322, 504, 526, 1483, 1553, 1918
MabI ACCWGGT 1 cut(s) 1377
MaeI CTAG 7 cut(s) 302, 476, 498, 683, 984, 1209, 1821
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 5 cut(s) 41, 880, 886, 1422, 1435
MalI GATC 6 cut(s) 450, 909, 1287, 1373, 1518, 1524
MboI GATC 6 cut(s) 448, 907, 1285, 1371, 1516, 1522
MboII GAAGA 9 cut(s) 107, 157, 341, 437, 458, 852, 1424, 1591, 1679
MfeI CAATTG 2 cut(s) 903, 1427
MflI RGATCY 3 cut(s) 448, 1285, 1516
MlsI TGGCCA 1 cut(s) 1007
MluNI TGGCCA 1 cut(s) 1007
Mly113I GGCGCC 1 cut(s) 1256
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 3 cut(s) 817, 1494, 1893
Mox20I TGGCCA 1 cut(s) 1007
Mph1103I ATGCAT 3 cut(s) 163, 519, 590
MroXI GAANNNNTTC 1 cut(s) 273
MscI TGGCCA 1 cut(s) 1007
MseI TTAA 5 cut(s) 246, 507, 809, 1614, 1649
MslI CAYNNNNRTG 1 cut(s) 975
Msp20I TGGCCA 1 cut(s) 1007
MspA1I CMGCKG 1 cut(s) 777
MspI CCGG 1 cut(s) 1906
MspR9I CCNGG 3 cut(s) 491, 1099, 1379
MteI GCGCNGCGC 1 cut(s) 1880
MunI CAATTG 2 cut(s) 903, 1427
MvaI CCWGG 3 cut(s) 491, 1099, 1379
MvnI CGCG 4 cut(s) 654, 1877, 1882, 1893
MwoI GCNNNNNNNGC 6 cut(s) 80, 1004, 1682, 1729, 1890, 1943
NarI GGCGCC 1 cut(s) 1256
NcoI CCATGG 2 cut(s) 1147, 1594
NdeII GATC 6 cut(s) 448, 907, 1285, 1371, 1516, 1522
NheI GCTAGC 1 cut(s) 301
NlaIII CATG 7 cut(s) 137, 544, 551, 1151, 1465, 1511, 1598
NlaIV GGNNCC 4 cut(s) 799, 1257, 1362, 1518
NmuCI GTSAC 4 cut(s) 41, 880, 1422, 1435
NsbI TGCGCA 1 cut(s) 1131
NsiI ATGCAT 3 cut(s) 163, 519, 590
NspI RCATGY 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 1485
PciI ACATGT 1 cut(s) 133
PcsI WCGNNNNNNNCGW 2 cut(s) 562, 1402
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 4 cut(s) 274, 574, 1560, 1569
PflMI CCANNNNNTGG 2 cut(s) 1148, 1639
PfoI TCCNGGA 1 cut(s) 489
PleI GAGTC 1 cut(s) 247
PluTI GGCGCC 1 cut(s) 1259
PpsI GAGTC 1 cut(s) 247
PpuMI RGGWCCY 1 cut(s) 1360
PscI ACATGT 1 cut(s) 133
PsiI TTATAA 1 cut(s) 1503
Psp5II RGGWCCY 1 cut(s) 1360
Psp6I CCWGG 3 cut(s) 489, 1097, 1377
PspFI CCCAGC 1 cut(s) 1538
PspGI CCWGG 3 cut(s) 489, 1097, 1377
PspN4I GGNNCC 4 cut(s) 799, 1257, 1362, 1518
PspPI GGNCC 4 cut(s) 487, 899, 1360, 1828
PspPPI RGGWCCY 1 cut(s) 1360
PspXI VCTCGAGB 1 cut(s) 1485
PstI CTGCAG 1 cut(s) 919
PsuI RGATCY 3 cut(s) 448, 1285, 1516
PvuII CAGCTG 1 cut(s) 777
RsaI GTAC 2 cut(s) 1819, 1917
RsaNI GTAC 2 cut(s) 1818, 1916
RseI CAYNNNNRTG 1 cut(s) 975
SaqAI TTAA 5 cut(s) 246, 507, 809, 1614, 1649
Sau3AI GATC 6 cut(s) 448, 907, 1285, 1371, 1516, 1522
Sau96I GGNCC 4 cut(s) 487, 899, 1360, 1828
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 3 cut(s) 491, 1099, 1379
SexAI ACCWGGT 1 cut(s) 1377
SfaNI GCATC 7 cut(s) 148, 322, 504, 526, 1483, 1553, 1918
SfcI CTRYAG 3 cut(s) 441, 663, 915
SfoI GGCGCC 1 cut(s) 1257
Sfr274I CTCGAG 1 cut(s) 1485
SinI GGWCC 4 cut(s) 487, 899, 1360, 1828
SlaI CTCGAG 1 cut(s) 1485
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 1 cut(s) 1485
SmoI CTYRAG 1 cut(s) 1485
SpeI ACTAGT 2 cut(s) 475, 1208
SsiI CCGC 6 cut(s) 654, 1253, 1676, 1875, 1880, 1944
SspDI GGCGCC 1 cut(s) 1255
SspI AATATT 2 cut(s) 170, 1756
SspMI CTAG 7 cut(s) 302, 476, 498, 683, 984, 1209, 1821
StyD4I CCNGG 3 cut(s) 489, 1097, 1377
StyI CCWWGG 5 cut(s) 846, 1008, 1028, 1147, 1594
TaaI ACNGT 3 cut(s) 12, 740, 886
TaiI ACGT 1 cut(s) 31
TaqI TCGA 8 cut(s) 381, 577, 874, 1187, 1213, 1374, 1486, 1912
TaqII GACCGA 1 cut(s) 1454
TatI WGTACW 1 cut(s) 1915
TauI GCSGC 2 cut(s) 1256, 1882
TfiI GAWTC 4 cut(s) 274, 574, 1560, 1569
Tru1I TTAA 5 cut(s) 246, 507, 809, 1614, 1649
Tru9I TTAA 5 cut(s) 246, 507, 809, 1614, 1649
TscAI CASTG 4 cut(s) 603, 755, 1287, 1440
TseFI GTSAC 4 cut(s) 41, 880, 1422, 1435
TseI GCWGC 9 cut(s) 19, 74, 77, 80, 774, 914, 1082, 1132, 1451
Tsp45I GTSAC 4 cut(s) 41, 880, 1422, 1435
TspDTI ATGAA 5 cut(s) 678, 798, 959, 1032, 1281
TspGWI ACGGA 1 cut(s) 977
TspRI CASTG 4 cut(s) 603, 755, 1287, 1440
Van91I CCANNNNNTGG 2 cut(s) 1148, 1639
VpaK11BI GGWCC 4 cut(s) 487, 899, 1360, 1828
XagI CCTNNNNNAGG 1 cut(s) 915
XapI RAATTY 3 cut(s) 56, 965, 1790
XceI RCATGY 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 774
XhoI CTCGAG 1 cut(s) 1485
XmiI GTMKAC 2 cut(s) 1172, 1528
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 7 cut(s) 302, 476, 498, 683, 984, 1209, 1821
Zsp2I ATGCAT 3 cut(s) 163, 519, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.