Rorug03G0306100

Coiled-coil regions of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005999
Physical Location & Seq
Forward (+)
32458592 .. 32460540
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0306100.1

Sequence Viewer

Length: 855 bp
ATGCGACCTCCCAGAGGCGGCGGTAGTTTCAGAGGCAACCGCGGTGGCGGAGGTTTCAGGGGCCGCGGCGGCGGGGACTTCAGAGGTGGCCGTGGTGGCTTCCGCGACGAAGGCCCTCCTTCCGAAGTGGTAGAGGTTTCGACGTTTGTTCATGCATGTGAGGGTGACGCTGTCACAAAGCTCACACATGAGAAGATACCCTATTTCAATGCCCCAATCTATCTGCAGAACAAGACTCAGATAGGCAAAGTTGATGAAATTTTTGGCCCAATTAATGAATCTTACTTCTCGGTCAAAGTGATGGAAGGCATTGTGGCAACTTCTTATTCACAGGGTGATAAGTTCTATATTGACCCAGCGAAGCTTCTTCCTCTTGCAAGATTTCTTCCACAGCCCAAGAAACTTCTTGGGAGCATTTGTTCTTTGTCATATCTACAATTGCCATTCCCGTTTCATGAGAACAGGAGATTCTTTGGGATTGTGTATTGGGGTCGAATGGGAATATCAGGGGACAGAAACCAGCTTTTTCTCGAGGTGGAGGACGTGGAGGCAGGGGACCTCCAAGGGGTGGCAGAGGTGGAGTTTTTCGTGGCAGGGGACCTCCAAGAGGGGGTGGCAGAGGACCCCCGAGGGGTGGGCGTGGTGGCTTCAGGGGCAGAGGGAGATTTTAGAGTAACATTGCCCTCCCCATTTCATGTTGTCGCTATTTCAGGATGCTTTAGGATTCACAAAAGTCACAGTTTCATGCTTGTTTTGGTGGTGACATGTGGTTTTGTGGATGTTCATGTTTATGTCTATGTTTATCTTGCTGAAGACTTTGTACTGGATGAAGTTAATTATAGATGTTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007623 GO:0008150 GO:0016020 GO:0044464 GO:0048511 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAB-ABD SCAB-IgPH SCAB_CC

Protein Analysis

284

Amino Acids

31.3

Weight (kDa)

5.68

Isoelectric Point (pI)

31.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gar1 PF04410 43 - 129 2e-27 Gar1/Naf1 RNA binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 568
AccII CGCG 3 cut(s) 42, 66, 105
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 1 cut(s) 258
AcuI CTGAAG 3 cut(s) 64, 633, 831
AdeI CACNNNGTG 1 cut(s) 335
AfaI GTAC 1 cut(s) 822
AfiI CCNNNNNNNGG 8 cut(s) 14, 17, 565, 568, 607, 610, 631, 634
AflIII ACRYGT 1 cut(s) 764
AgsI TTSAA 1 cut(s) 208
AjiI CACGTC 1 cut(s) 544
AloI GAACNNNNNNTCC 2 cut(s) 403, 435
AluBI AGCT 3 cut(s) 181, 364, 523
AluI AGCT 3 cut(s) 181, 364, 523
Ama87I CYCGRG 2 cut(s) 530, 627
AoxI GGCC 4 cut(s) 61, 88, 112, 265
ApoI RAATTY 1 cut(s) 258
AseI ATTAAT 1 cut(s) 273
AspS9I GGNCC 6 cut(s) 61, 113, 266, 556, 598, 622
AsuHPI GGTGA 3 cut(s) 176, 347, 772
AvaI CYCGRG 2 cut(s) 530, 627
AvaII GGWCC 3 cut(s) 556, 598, 622
BarI GAAGNNNNNNTAC 2 cut(s) 804, 836
BbsI GAAGAC 1 cut(s) 819
BccI CCATC 1 cut(s) 295
BceAI ACGGC 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 224
BglI GCCNNNNNGGC 2 cut(s) 69, 96
BisI GCNGC 4 cut(s) 19, 64, 67, 70
BlsI GCNGC 4 cut(s) 20, 65, 68, 71
Bme18I GGWCC 3 cut(s) 556, 598, 622
BmeT110I CYCGRG 2 cut(s) 530, 627
BmgBI CACGTC 1 cut(s) 544
BmgT120I GGNCC 6 cut(s) 61, 113, 266, 556, 598, 622
BmiI GGNNCC 4 cut(s) 62, 557, 599, 624
BmsI GCATC 1 cut(s) 704
BpiI GAAGAC 1 cut(s) 819
BsaJI CCNNGG 5 cut(s) 40, 64, 91, 562, 628
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 8 cut(s) 14, 17, 565, 568, 607, 610, 631, 634
Bse1I ACTGG 1 cut(s) 828
Bse3DI GCAATG 1 cut(s) 677
BseDI CCNNGG 5 cut(s) 40, 64, 91, 562, 628
BseGI GGATG 3 cut(s) 719, 784, 832
BseLI CCNNNNNNNGG 8 cut(s) 14, 17, 565, 568, 607, 610, 631, 634
BseMI GCAATG 1 cut(s) 677
BseMII CTCAG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 828
BseYI CCCAGC 1 cut(s) 355
Bsh1236I CGCG 3 cut(s) 42, 66, 105
BshFI GGCC 4 cut(s) 63, 90, 114, 267
BsiHKCI CYCGRG 2 cut(s) 530, 627
BslFI GGGAC 4 cut(s) 89, 524, 569, 611
BslI CCNNNNNNNGG 8 cut(s) 14, 17, 565, 568, 607, 610, 631, 634
BsmFI GGGAC 4 cut(s) 89, 524, 569, 611
BsnI GGCC 4 cut(s) 63, 90, 114, 267
BsoBI CYCGRG 2 cut(s) 530, 627
BspANI GGCC 4 cut(s) 63, 90, 114, 267
BspCNI CTCAG 1 cut(s) 250
BspFNI CGCG 3 cut(s) 42, 66, 105
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 4 cut(s) 62, 557, 599, 624
BspMAI CTGCAG 1 cut(s) 228
BsrDI GCAATG 1 cut(s) 677
BsrI ACTGG 1 cut(s) 828
BssECI CCNNGG 5 cut(s) 40, 64, 91, 562, 628
BssT1I CCWWGG 1 cut(s) 562
Bst4CI ACNGT 1 cut(s) 740
BstDEI CTNAG 1 cut(s) 237
BstDSI CCRYGG 3 cut(s) 40, 64, 91
BstENI CCTNNNNNAGG 2 cut(s) 12, 605
BstF5I GGATG 3 cut(s) 719, 784, 832
BstFNI CGCG 3 cut(s) 42, 66, 105
BstMWI GCNNNNNNNGC 4 cut(s) 69, 96, 111, 653
BstNSI RCATGY 2 cut(s) 159, 768
BstSFI CTRYAG 1 cut(s) 224
BstUI CGCG 3 cut(s) 42, 66, 105
BstV2I GAAGAC 1 cut(s) 819
BsuRI GGCC 4 cut(s) 63, 90, 114, 267
BtgI CCRYGG 3 cut(s) 40, 64, 91
BtrI CACGTC 1 cut(s) 544
BtsCI GGATG 3 cut(s) 719, 784, 832
CciI TCATGA 1 cut(s) 454
Cfr13I GGNCC 6 cut(s) 61, 113, 266, 556, 598, 622
Cfr42I CCGCGG 2 cut(s) 43, 67
CseI GACGC 1 cut(s) 176
Csp6I GTAC 1 cut(s) 821
CspCI CAANNNNNGTGG 2 cut(s) 25, 60
CviAII CATG 8 cut(s) 152, 156, 188, 455, 695, 745, 765, 785
CviQI GTAC 1 cut(s) 821
DdeI CTNAG 1 cut(s) 237
DraIII CACNNNGTG 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 88
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 562
Eco47I GGWCC 3 cut(s) 556, 598, 622
Eco57I CTGAAG 3 cut(s) 64, 633, 831
Eco88I CYCGRG 2 cut(s) 530, 627
EcoNI CCTNNNNNAGG 2 cut(s) 12, 605
EcoO109I RGGNCCY 4 cut(s) 113, 556, 598, 622
EcoT14I CCWWGG 1 cut(s) 562
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 562
FaeI CATG 8 cut(s) 155, 159, 191, 458, 698, 748, 768, 788
FaqI GGGAC 4 cut(s) 89, 524, 569, 611
FatI CATG 8 cut(s) 151, 155, 187, 454, 694, 744, 764, 784
FauI CCCGC 1 cut(s) 65
Fnu4HI GCNGC 4 cut(s) 19, 64, 67, 70
FokI GGATG 3 cut(s) 726, 791, 839
Fsp4HI GCNGC 4 cut(s) 19, 64, 67, 70
GluI GCNGC 4 cut(s) 19, 64, 67, 70
GsaI CCCAGC 1 cut(s) 359
HaeIII GGCC 4 cut(s) 63, 90, 114, 267
HgaI GACGC 1 cut(s) 176
Hin1II CATG 8 cut(s) 155, 159, 191, 458, 698, 748, 768, 788
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 4 cut(s) 235, 278, 468, 724
HphI GGTGA 3 cut(s) 176, 347, 772
Hpy188I TCNGA 4 cut(s) 32, 83, 124, 240
Hpy188III TCNNGA 3 cut(s) 455, 530, 711
Hpy99I CGWCG 2 cut(s) 110, 145
HpyAV CCTTC 3 cut(s) 104, 129, 299
HpyCH4III ACNGT 1 cut(s) 740
HpyCH4IV ACGT 2 cut(s) 143, 543
HpyCH4V TGCA 3 cut(s) 155, 226, 377
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 96, 111, 653
HpyF3I CTNAG 1 cut(s) 237
HpySE526I ACGT 2 cut(s) 143, 543
Hsp92II CATG 8 cut(s) 155, 159, 191, 458, 698, 748, 768, 788
KspI CCGCGG 2 cut(s) 43, 67
LmnI GCTCC 1 cut(s) 411
LweI GCATC 1 cut(s) 704
MaeII ACGT 2 cut(s) 143, 543
MaeIII GTNAC 5 cut(s) 164, 172, 673, 734, 760
MboII GAAGA 4 cut(s) 205, 359, 377, 824
MfeI CAATTG 1 cut(s) 437
MluCI AATT 4 cut(s) 258, 270, 437, 835
MlyI GAGTC 1 cut(s) 229
Mph1103I ATGCAT 1 cut(s) 157
MseI TTAA 2 cut(s) 273, 834
MslI CAYNNNNRTG 2 cut(s) 156, 789
MspA1I CMGCKG 2 cut(s) 42, 66
MunI CAATTG 1 cut(s) 437
MvnI CGCG 3 cut(s) 42, 66, 105
MwoI GCNNNNNNNGC 4 cut(s) 69, 96, 111, 653
NlaIII CATG 8 cut(s) 155, 159, 191, 458, 698, 748, 768, 788
NlaIV GGNNCC 4 cut(s) 62, 557, 599, 624
NmuCI GTSAC 4 cut(s) 164, 172, 734, 760
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 2 cut(s) 159, 768
PaeR7I CTCGAG 1 cut(s) 530
PagI TCATGA 1 cut(s) 454
PciI ACATGT 1 cut(s) 764
PfeI GAWTC 3 cut(s) 278, 468, 724
PflFI GACNNNGTC 1 cut(s) 170
PflMI CCANNNNNTGG 1 cut(s) 568
PkrI GCNGC 4 cut(s) 20, 65, 68, 71
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PpuMI RGGWCCY 3 cut(s) 556, 598, 622
PscI ACATGT 1 cut(s) 764
PshBI ATTAAT 1 cut(s) 273
Psp5II RGGWCCY 3 cut(s) 556, 598, 622
PspFI CCCAGC 1 cut(s) 355
PspN4I GGNNCC 4 cut(s) 62, 557, 599, 624
PspPI GGNCC 6 cut(s) 61, 113, 266, 556, 598, 622
PspPPI RGGWCCY 3 cut(s) 556, 598, 622
PstI CTGCAG 1 cut(s) 228
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 1 cut(s) 822
RsaNI GTAC 1 cut(s) 821
RseI CAYNNNNRTG 2 cut(s) 156, 789
SacII CCGCGG 2 cut(s) 43, 67
SaqAI TTAA 2 cut(s) 273, 834
SatI GCNGC 4 cut(s) 19, 64, 67, 70
Sau96I GGNCC 6 cut(s) 61, 113, 266, 556, 598, 622
SchI GAGTC 1 cut(s) 229
SfaNI GCATC 1 cut(s) 704
SfcI CTRYAG 1 cut(s) 224
Sfr274I CTCGAG 1 cut(s) 530
Sfr303I CCGCGG 2 cut(s) 43, 67
SgrBI CCGCGG 2 cut(s) 43, 67
SinI GGWCC 3 cut(s) 556, 598, 622
SlaI CTCGAG 1 cut(s) 530
SmiMI CAYNNNNRTG 2 cut(s) 156, 789
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 4 cut(s) 258, 270, 437, 835
StyI CCWWGG 1 cut(s) 562
TaaI ACNGT 1 cut(s) 740
TaiI ACGT 2 cut(s) 146, 546
TaqI TCGA 3 cut(s) 140, 493, 531
TaqII GACCGA 1 cut(s) 280
TasI AATT 4 cut(s) 258, 270, 437, 835
TatI WGTACW 1 cut(s) 820
TauI GCSGC 4 cut(s) 21, 66, 69, 72
TfiI GAWTC 3 cut(s) 278, 468, 724
Tru1I TTAA 2 cut(s) 273, 834
Tru9I TTAA 2 cut(s) 273, 834
TseFI GTSAC 4 cut(s) 164, 172, 734, 760
Tsp45I GTSAC 4 cut(s) 164, 172, 734, 760
TspDTI ATGAA 8 cut(s) 140, 270, 291, 443, 683, 733, 773, 843
Tth111I GACNNNGTC 1 cut(s) 170
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 3 cut(s) 556, 598, 622
VspI ATTAAT 1 cut(s) 273
XagI CCTNNNNNAGG 2 cut(s) 12, 605
XapI RAATTY 1 cut(s) 258
XceI RCATGY 2 cut(s) 159, 768
XhoI CTCGAG 1 cut(s) 530
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.