Rh4CG010500

Coiled-coil regions of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005999
Physical Location & Seq
Forward (+)
1828087 .. 1839136
11050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG010500.1

Sequence Viewer

Length: 1476 bp
ATGACGAAGATAAGTCCTGAATTCGAAGAGAATCTCACAACAGAGGGAGTTCTTTCAGTTTCAGCTGATGTCAGCTTCATTTCTAATGGTTTCCCAAAGTACAGACTAGGACCTGATAATCAGATTCTGGATGAGCCGAAGGGGGGTAGCGATGGTCCAACCTTAAAAGATGTAGTTGAAGAAGAAATGACCCAACTGTCAGAGCAGCATAAACGTCTTTCAGTTCGTGACCTTGCCAGTAAATTTGACAAGAACTTGGCTGCTGCTGCTAAATTGTCTGAAGAGGCAAAAATTAGAGAAGTGGCTTCTTTGGAGGGACATGTTCTTTTGAAGAAGCTGAGAGATGCATTGGAATATTTGAGAGGGCTTTTAGCAGGGAGAAACAAGGAGGATGTGGAGGAAGCTATTTCTATGGTGGAGGCTTTAGCCGTTAAGTTGACTCAAAAGGAAGGAGAATTGATTCAGGACAAGTTTGAAGTGAAAAAGCTAGCCAGCTTTCTCAAACAGACTTCTGAAGATGCTAAAAAGCTGGTTAGCCAAGAGAAATCTTTTGCCTGTGCTGAAATCGAAAGTGCAAGGGGAATAGTCAAGAGAATTGGAGAGGCTCTTGAAGAACGAAACAGAACTACAGAAGATCCTAAAAAACAGGATGTGGAGGAACTAGTTGAGGTGGTCCAGGAAGCTAGGAGAATTAAATTGATGCATCAGCCAAGCAAGGTGATGGACATGGAACATGAGCTTCGTGCATTACGAACACAGATTCGAGAGAAATGCATAGTTTCAGTGAAGCTTCAGAAAGAGCTGGCAATGAGCAAGAGGGCAAAGGAGAACAAATCTCGCGGATACGCTATAGTTGGTTCTGAAACTCTAGGTTCATATCTACAAATCCAAACTTGCTCAGAAAATGTTCCACAGCTTTCAAACTGCTCAATCCAGTGGTACCGTGTTTCACCAGATGGCAGCTGGAATGAAGCTATTTCAGGTGCCAATATATTAACTTATGCTCCAGAGCCTTTTGATGTTGGTAGAACCTTGGGTGCAGACATTATTTCCAATGGTCAAAAAGTGACAGTTACAACTGCTGGTCCAATTGATCCTGCTGCAGGACTTGAAAGCTACATAGACACACTTTTGCGAAAATCTAACACGGAATTTCATGTTGTTATTTCACAAATGAATGGGCGTGATCATCCATCACGTTCTGTTCATGTATTTCATGTGGGGAAGATGAGGATGAAGCTTTGTAGAGGATGGATTACAAAGTCTAGAGAAAATTATTCCTCGTTGATGCAGCTATGTGGCGCTAGAGGTGATGTTGCTAATGTGGCCAAGGGAGTGTTTTGGCAACCAAGGAAGGGTCTTTCATTTGTATTGACATTTGAAACAGAGCGAGATAGAAATGCAGCAATACTTCTTGCCAGGAAATATGCTCTTGATTGCAATGTCATGATTGCTGGACCAGATGATAGAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007623 GO:0008150 GO:0016020 GO:0044464 GO:0048511 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAB-ABD SCAB-IgPH SCAB_CC

Protein Analysis

491

Amino Acids

54.8

Weight (kDa)

7.09

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 55 - 97 1.8e-23 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 101 - 268 1.3e-72 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 282 - 379 8.9e-43 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 382 - 489 2.8e-55 PH domain of plant-specific actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 196
Acc65I GGTACC 1 cut(s) 939
AccB1I GGYRCC 2 cut(s) 939, 983
AccII CGCG 1 cut(s) 840
AciI CCGC 1 cut(s) 840
AclWI GGATC 2 cut(s) 629, 1088
AcoI YGGCCR 1 cut(s) 1326
AcsI RAATTY 3 cut(s) 20, 242, 1151
AcuI CTGAAG 3 cut(s) 300, 534, 776
AfaI GTAC 2 cut(s) 101, 941
AfiI CCNNNNNNNGG 3 cut(s) 143, 1103, 1355
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 7 cut(s) 179, 331, 476, 611, 921, 1112, 1382
AhlI ACTAGT 1 cut(s) 661
AjnI CCWGG 2 cut(s) 675, 1418
AlwI GGATC 2 cut(s) 629, 1088
AlwNI CAGNNNCTG 1 cut(s) 127
AoxI GGCC 1 cut(s) 1326
ApeKI GCWGC 8 cut(s) 205, 260, 263, 266, 960, 1100, 1291, 1403
ApoI RAATTY 3 cut(s) 20, 242, 1151
Asp700I GAANNNNTTC 2 cut(s) 459, 906
Asp718I GGTACC 1 cut(s) 939
AspLEI GCGC 1 cut(s) 1304
AspS9I GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
AsuHPI GGTGA 3 cut(s) 730, 942, 1322
AsuII TTCGAA 1 cut(s) 24
AsuNHI GCTAGC 1 cut(s) 487
AvaII GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
BalI TGGCCA 1 cut(s) 1328
BanI GGYRCC 2 cut(s) 939, 983
BbvI GCAGC 8 cut(s) 217, 247, 250, 253, 972, 1087, 1303, 1415
BccI CCATC 5 cut(s) 146, 715, 950, 1201, 1245
BceAI ACGGC 1 cut(s) 413
BcgI CGANNNNNNTGC 2 cut(s) 753, 787
BciT130I CCWGG 2 cut(s) 677, 1420
BciVI GTATCC 1 cut(s) 836
BclI TGATCA 1 cut(s) 1186
BcuI ACTAGT 1 cut(s) 661
BfaI CTAG 7 cut(s) 107, 488, 662, 684, 869, 1266, 1305
BfmI CTRYAG 3 cut(s) 627, 849, 1101
BfoI RGCGCY 1 cut(s) 1305
BfuI GTATCC 1 cut(s) 836
BisI GCNGC 8 cut(s) 206, 261, 264, 267, 961, 1101, 1292, 1404
BlsI GCNGC 8 cut(s) 207, 262, 265, 268, 962, 1102, 1293, 1405
Bme1390I CCNGG 2 cut(s) 677, 1420
Bme18I GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
BmgT120I GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
BmiI GGNNCC 2 cut(s) 941, 985
BmrFI CCNGG 2 cut(s) 677, 1420
BmsI GCATC 5 cut(s) 334, 508, 690, 712, 1278
BmtI GCTAGC 1 cut(s) 491
BpmI CTGGAG 1 cut(s) 990
Bpu14I TTCGAA 1 cut(s) 24
BsaJI CCNNGG 3 cut(s) 1032, 1329, 1349
BsaXI ACNNNNNCTCC 2 cut(s) 988, 1018
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 1103, 1355
Bse1I ACTGG 2 cut(s) 237, 934
Bse3DI GCAATG 2 cut(s) 813, 1447
BseBI CCWGG 2 cut(s) 677, 1420
BseDI CCNNGG 3 cut(s) 1032, 1329, 1349
BseGI GGATG 6 cut(s) 136, 397, 655, 1189, 1239, 1256
BseLI CCNNNNNNNGG 3 cut(s) 143, 1103, 1355
BseMI GCAATG 2 cut(s) 813, 1447
BseMII CTCAG 2 cut(s) 329, 912
BseNI ACTGG 2 cut(s) 237, 934
BseXI GCAGC 8 cut(s) 217, 247, 250, 253, 972, 1087, 1303, 1415
BsgI GTGCAG 1 cut(s) 1059
Bsh1236I CGCG 1 cut(s) 840
BshFI GGCC 1 cut(s) 1328
BshNI GGYRCC 2 cut(s) 939, 983
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 3 cut(s) 143, 1103, 1355
BsmFI GGGAC 1 cut(s) 330
BsnI GGCC 1 cut(s) 1328
Bsp119I TTCGAA 1 cut(s) 24
Bsp143I GATC 3 cut(s) 634, 1093, 1186
BspACI CCGC 1 cut(s) 840
BspANI GGCC 1 cut(s) 1328
BspCNI CTCAG 2 cut(s) 330, 911
BspFNI CGCG 1 cut(s) 840
BspHI TCATGA 1 cut(s) 1446
BspLI GGNNCC 2 cut(s) 941, 985
BspMAI CTGCAG 1 cut(s) 1105
BspOI GCTAGC 1 cut(s) 491
BspPI GGATC 2 cut(s) 629, 1088
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 2 cut(s) 939, 983
BsrDI GCAATG 2 cut(s) 813, 1447
BsrI ACTGG 2 cut(s) 237, 934
BssECI CCNNGG 3 cut(s) 1032, 1329, 1349
BssMI GATC 3 cut(s) 634, 1093, 1186
BssT1I CCWWGG 3 cut(s) 1032, 1329, 1349
Bst2UI CCWGG 2 cut(s) 677, 1420
Bst4CI ACNGT 3 cut(s) 198, 944, 1072
Bst6I CTCTTC 2 cut(s) 21, 276
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 3 cut(s) 489, 493, 804
BstDEI CTNAG 2 cut(s) 338, 898
BstENI CCTNNNNNAGG 1 cut(s) 1101
BstF5I GGATG 6 cut(s) 136, 397, 655, 1189, 1239, 1256
BstFNI CGCG 1 cut(s) 840
BstH2I RGCGCY 1 cut(s) 1305
BstHHI GCGC 1 cut(s) 1304
BstKTI GATC 3 cut(s) 637, 1096, 1189
BstMBI GATC 3 cut(s) 634, 1093, 1186
BstMWI GCNNNNNNNGC 2 cut(s) 266, 1325
BstNI CCWGG 2 cut(s) 677, 1420
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 2 cut(s) 675, 1418
BstSFI CTRYAG 3 cut(s) 627, 849, 1101
BstUI CGCG 1 cut(s) 840
BstV1I GCAGC 8 cut(s) 217, 247, 250, 253, 972, 1087, 1303, 1415
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 1 cut(s) 1328
BtgZI GCGATG 1 cut(s) 165
BtsCI GGATG 6 cut(s) 136, 397, 655, 1189, 1239, 1256
BtsIMutI CAGTG 2 cut(s) 789, 941
Cac8I GCNNGC 3 cut(s) 489, 493, 804
CaiI CAGNNNCTG 1 cut(s) 127
CciI TCATGA 1 cut(s) 1446
CfoI GCGC 1 cut(s) 1304
Cfr13I GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
Csp6I GTAC 2 cut(s) 100, 940
CviAII CATG 7 cut(s) 320, 727, 734, 1157, 1208, 1217, 1447
CviQI GTAC 2 cut(s) 100, 940
DdeI CTNAG 2 cut(s) 338, 898
DpnI GATC 3 cut(s) 636, 1095, 1188
DpnII GATC 3 cut(s) 634, 1093, 1186
DrdI GACNNNNNNGTC 1 cut(s) 196
DseDI GACNNNNNNGTC 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 1326
Eam1104I CTCTTC 2 cut(s) 21, 276
EarI CTCTTC 2 cut(s) 21, 276
Eco130I CCWWGG 3 cut(s) 1032, 1329, 1349
Eco47I GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
Eco57I CTGAAG 3 cut(s) 300, 534, 776
EcoNI CCTNNNNNAGG 1 cut(s) 1101
EcoO109I RGGNCCY 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 675, 1418
EcoT14I CCWWGG 3 cut(s) 1032, 1329, 1349
EcoT22I ATGCAT 3 cut(s) 349, 705, 776
ErhI CCWWGG 3 cut(s) 1032, 1329, 1349
FaeI CATG 7 cut(s) 323, 730, 737, 1160, 1211, 1220, 1450
FaqI GGGAC 1 cut(s) 330
FatI CATG 7 cut(s) 319, 726, 733, 1156, 1207, 1216, 1446
FbaI TGATCA 1 cut(s) 1186
Fnu4HI GCNGC 8 cut(s) 206, 261, 264, 267, 961, 1101, 1292, 1404
FokI GGATG 6 cut(s) 143, 404, 662, 1176, 1246, 1263
Fsp4HI GCNGC 8 cut(s) 206, 261, 264, 267, 961, 1101, 1292, 1404
FspBI CTAG 7 cut(s) 107, 488, 662, 684, 869, 1266, 1305
GlaI GCGC 1 cut(s) 1303
GluI GCNGC 8 cut(s) 206, 261, 264, 267, 961, 1101, 1292, 1404
GsuI CTGGAG 1 cut(s) 990
HaeII RGCGCY 1 cut(s) 1305
HaeIII GGCC 1 cut(s) 1328
HhaI GCGC 1 cut(s) 1304
Hin1II CATG 7 cut(s) 323, 730, 737, 1160, 1211, 1220, 1450
Hin6I GCGC 1 cut(s) 1302
HinP1I GCGC 1 cut(s) 1302
HincII GTYRAC 1 cut(s) 438
HindII GTYRAC 1 cut(s) 438
HindIII AAGCTT 2 cut(s) 788, 1238
HinfI GANTC 5 cut(s) 31, 124, 439, 460, 760
HphI GGTGA 3 cut(s) 730, 942, 1322
Hpy166II GTNNAC 1 cut(s) 438
Hpy188I TCNGA 7 cut(s) 123, 202, 280, 514, 795, 862, 901
Hpy8I GTNNAC 1 cut(s) 438
HpyAV CCTTC 3 cut(s) 133, 443, 1348
HpyCH4III ACNGT 3 cut(s) 198, 944, 1072
HpyCH4IV ACGT 2 cut(s) 214, 1198
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 1325
HpyF3I CTNAG 2 cut(s) 338, 898
HpySE526I ACGT 2 cut(s) 214, 1198
Hsp92II CATG 7 cut(s) 323, 730, 737, 1160, 1211, 1220, 1450
HspAI GCGC 1 cut(s) 1302
KpnI GGTACC 1 cut(s) 943
Ksp22I TGATCA 1 cut(s) 1186
Kzo9I GATC 3 cut(s) 634, 1093, 1186
LmnI GCTCC 1 cut(s) 1009
Lsp1109I GCAGC 8 cut(s) 217, 247, 250, 253, 972, 1087, 1303, 1415
LweI GCATC 5 cut(s) 334, 508, 690, 712, 1278
MaeI CTAG 7 cut(s) 107, 488, 662, 684, 869, 1266, 1305
MaeII ACGT 2 cut(s) 214, 1198
MaeIII GTNAC 3 cut(s) 227, 1066, 1072
MalI GATC 3 cut(s) 636, 1095, 1188
MboI GATC 3 cut(s) 634, 1093, 1186
MfeI CAATTG 1 cut(s) 1089
MflI RGATCY 1 cut(s) 634
MlsI TGGCCA 1 cut(s) 1328
MluNI TGGCCA 1 cut(s) 1328
MlyI GAGTC 1 cut(s) 433
MmeI TCCRAC 1 cut(s) 182
Mox20I TGGCCA 1 cut(s) 1328
Mph1103I ATGCAT 3 cut(s) 349, 705, 776
MroXI GAANNNNTTC 2 cut(s) 459, 906
MscI TGGCCA 1 cut(s) 1328
MseI TTAA 4 cut(s) 164, 432, 693, 995
Msp20I TGGCCA 1 cut(s) 1328
MspA1I CMGCKG 2 cut(s) 65, 963
MspR9I CCNGG 2 cut(s) 677, 1420
MunI CAATTG 1 cut(s) 1089
MvaI CCWGG 2 cut(s) 677, 1420
MvnI CGCG 1 cut(s) 840
MwoI GCNNNNNNNGC 2 cut(s) 266, 1325
NdeII GATC 3 cut(s) 634, 1093, 1186
NheI GCTAGC 1 cut(s) 487
NlaIII CATG 7 cut(s) 323, 730, 737, 1160, 1211, 1220, 1450
NlaIV GGNNCC 2 cut(s) 941, 985
NmuCI GTSAC 2 cut(s) 227, 1066
NsiI ATGCAT 3 cut(s) 349, 705, 776
NspI RCATGY 1 cut(s) 323
NspV TTCGAA 1 cut(s) 24
PagI TCATGA 1 cut(s) 1446
PciI ACATGT 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 748
PdmI GAANNNNTTC 2 cut(s) 459, 906
PfeI GAWTC 4 cut(s) 31, 124, 460, 760
PfoI TCCNGGA 1 cut(s) 675
PkrI GCNGC 8 cut(s) 207, 262, 265, 268, 962, 1102, 1293, 1405
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
PpuMI RGGWCCY 1 cut(s) 110
PscI ACATGT 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 675, 1418
PspGI CCWGG 2 cut(s) 675, 1418
PspN4I GGNNCC 2 cut(s) 941, 985
PspPI GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
PspPPI RGGWCCY 1 cut(s) 110
PstI CTGCAG 1 cut(s) 1105
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 634
PvuII CAGCTG 2 cut(s) 65, 963
RsaI GTAC 2 cut(s) 101, 941
RsaNI GTAC 2 cut(s) 100, 940
SaqAI TTAA 4 cut(s) 164, 432, 693, 995
SatI GCNGC 8 cut(s) 206, 261, 264, 267, 961, 1101, 1292, 1404
Sau3AI GATC 3 cut(s) 634, 1093, 1186
Sau96I GGNCC 5 cut(s) 110, 155, 673, 1085, 1457
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 2 cut(s) 677, 1420
SfaNI GCATC 5 cut(s) 334, 508, 690, 712, 1278
SfcI CTRYAG 3 cut(s) 627, 849, 1101
SfuI TTCGAA 1 cut(s) 24
SinI GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
SpeI ACTAGT 1 cut(s) 661
SsiI CCGC 1 cut(s) 840
SspI AATATT 1 cut(s) 356
SspMI CTAG 7 cut(s) 107, 488, 662, 684, 869, 1266, 1305
StyD4I CCNGG 2 cut(s) 675, 1418
StyI CCWWGG 3 cut(s) 1032, 1329, 1349
TaaI ACNGT 3 cut(s) 198, 944, 1072
TaiI ACGT 2 cut(s) 217, 1201
TaqI TCGA 3 cut(s) 24, 567, 763
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 4 cut(s) 31, 124, 460, 760
Tru1I TTAA 4 cut(s) 164, 432, 693, 995
Tru9I TTAA 4 cut(s) 164, 432, 693, 995
TscAI CASTG 2 cut(s) 789, 941
TseFI GTSAC 2 cut(s) 227, 1066
TseI GCWGC 8 cut(s) 205, 260, 263, 266, 960, 1100, 1291, 1403
Tsp45I GTSAC 2 cut(s) 227, 1066
TspDTI ATGAA 9 cut(s) 67, 864, 984, 1145, 1190, 1196, 1205, 1250, 1353
TspGWI ACGGA 1 cut(s) 1163
TspRI CASTG 2 cut(s) 789, 941
VpaK11BI GGWCC 5 cut(s) 110, 155, 673, 1085, 1457
XagI CCTNNNNNAGG 1 cut(s) 1101
XapI RAATTY 3 cut(s) 20, 242, 1151
XbaI TCTAGA 1 cut(s) 1265
XceI RCATGY 1 cut(s) 323
XcmI CCANNNNNNNNNTGG 1 cut(s) 960
XmnI GAANNNNTTC 2 cut(s) 459, 906
XspI CTAG 7 cut(s) 107, 488, 662, 684, 869, 1266, 1305
Zsp2I ATGCAT 3 cut(s) 349, 705, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.