MD12G1093800.v1.1

Coiled-coil regions of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005999
Physical Location & Seq
Forward (+)
12377984 .. 12389319
11336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1093800.v1.1.491

Sequence Viewer

Length: 1476 bp
ATGACAAAGATAAGTCCTGCATTTGAAGAGGAGATCCCAATTGAGGGAGTTCTGTCAGTTTCAGCTGATGTGAGCTTCATCTCTGATGGTTTTCCTAAGTATAAATTAGGACCTGATAATCAGATACTGGAAGAGCCCAAGGAGAATAGTGACGGTCCGACTGTGAAAGAGGTAGTTGAACAGGAAACTACCCATTTGTCAGAACAGCATAAACGTCTTTCAGTTCGTGACCTTGCCAGTAAATTTGACAAGAACTTGGTTGCTGCTGCTAAATTATCTGAAGAGGCAAAGCTTAGAGAAGTGGCTTCTTTGGAGGGACATGTTCTTTTGAAGAAGCTTAGAGATGCATTGGAACATTTGAGAGGGCGCTTGGCAGGGCGAAATAAGGAGGATGTGGAGAAAGCTATTTCTATGGTGGAAGCTTTAGCTGTTAAGTTGACTCAAAAGGAAGGAGAACTGATTCAGGAGAAGTTTGAAGTGAAAAAGCTGGCTAGTTTTCTCAAACAGGCATCTGAAGATGCTAAAAAGTTGGTTAACCAAGAGAAATCTTTTGCTTGTGCTGAAATTGAAAGTGCGAGGGCAGTAGTACAGAGAATTGGAGAGGCTCTTGAAGAGCAAGACATAACTTTTGAAGCTTCTAAAAAACAGGATGTGGAGGAACTAGTTGAGGTGGTTCAGGAAGCTAGGAGAATTAAGTTAATGCATCAGCCAAGCAAGGTAATGGACATGGAGCATGAGCTTCGTGCACTACGAACTCAGATACGAGAGAAATGCATAGTTTCAGTGAAGCTTCAGAAAGAGCTGGCAATGAACAAGAGAGCGGAGGAAAACAAATCTAGTTTATATCTTATAGATGGTTCAGAAACTCTAGGTGCATATCTGCGAATCCAACCTGTCTCAGAAAATGCTCCACAACTTTCAAACTGTGCAATTCAATGGTACCGCGTTTCATCAGATGGTAGCAGGAATGAAGCTATTTCAGGTGCCAACAAGTCTATTTATGCTCCAGAACCCTTTGATGTTGGACGAATCCTACAAGCAGACATAATTTCCAATGACCAAAGAGTCATAGTGATGACTGCTGGTCCTATTGATCCTGCTGCAGGAATCGGAAGCTATGTAGACACACTTTTGCGAAAATCTAACACTGAATTTAATGTAGTTATTTCAAAAATGAATGGACAAGACCATACATCGCATTCTGTTCATGCATTCCATGTTGGGAAGATGAGGATGAAGCTCAGTAGAGGATGGATTACCAAGTCGAGAGAAAACTATTCCTCTTCAATGCAGCTATGTGGCGTTAGAGGTGATATTGCAAATGCGGCGAAGGCATTGTTCTGGCAAGCAAGGAAGGGCCTCTCATTTGTACTGACATTTGAAACAGAGAGGGAAAGAAATGCAGCCATTATACTTGCCAGGAAATATGCTCTTGATTGCAATATCATGCTTGCCGGACCAGATGACAGAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007623 GO:0008150 GO:0016020 GO:0044464 GO:0048511 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAB-ABD SCAB-IgPH SCAB_CC

Protein Analysis

492

Amino Acids

54.86

Weight (kDa)

6.43

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 55 - 97 3.3e-24 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 101 - 268 2.7e-75 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 282 - 379 2.4e-45 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 382 - 489 4.5e-58 PH domain of plant-specific actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1064
Acc65I GGTACC 1 cut(s) 939
AccB1I GGYRCC 2 cut(s) 939, 983
AccBSI CCGCTC 1 cut(s) 821
AccI GTMKAC 1 cut(s) 1122
AccII CGCG 1 cut(s) 945
AciI CCGC 3 cut(s) 821, 943, 1325
AclWI GGATC 2 cut(s) 28, 1088
AcsI RAATTY 2 cut(s) 242, 1151
AcuI CTGAAG 3 cut(s) 300, 534, 776
AfaI GTAC 3 cut(s) 588, 941, 1371
AfiI CCNNNNNNNGG 3 cut(s) 43, 44, 1103
AflIII ACRYGT 1 cut(s) 319
AhdI GACNNNNNGTC 1 cut(s) 1083
AhlI ACTAGT 1 cut(s) 661
AjnI CCWGG 1 cut(s) 1418
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 1 cut(s) 901
Alw44I GTGCAC 1 cut(s) 744
AlwI GGATC 2 cut(s) 28, 1088
AlwNI CAGNNNCTG 1 cut(s) 127
AoxI GGCC 1 cut(s) 1357
ApaLI GTGCAC 1 cut(s) 744
ApeKI GCWGC 5 cut(s) 263, 266, 1100, 1291, 1403
ApoI RAATTY 2 cut(s) 242, 1151
ArsI GACNNNNNNTTYG 2 cut(s) 611, 643
Asp700I GAANNNNTTC 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 939
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 5 cut(s) 110, 155, 1085, 1357, 1457
AsuHPI GGTGA 1 cut(s) 1322
AvaII GGWCC 4 cut(s) 110, 155, 1085, 1457
BaeGI GKGCMC 1 cut(s) 748
BanI GGYRCC 2 cut(s) 939, 983
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 748
BbvI GCAGC 5 cut(s) 250, 253, 1087, 1303, 1415
BccI CCATC 4 cut(s) 80, 848, 950, 1245
BcgI CGANNNNNNTGC 4 cut(s) 722, 753, 756, 787
BciT130I CCWGG 1 cut(s) 1420
BcoDI GTCTC 1 cut(s) 901
BcuI ACTAGT 1 cut(s) 661
BfaI CTAG 5 cut(s) 492, 662, 684, 837, 869
BfmI CTRYAG 1 cut(s) 1101
BfoI RGCGCY 1 cut(s) 370
BisI GCNGC 6 cut(s) 264, 267, 1101, 1292, 1326, 1404
BlsI GCNGC 6 cut(s) 265, 268, 1102, 1293, 1327, 1405
Bme1390I CCNGG 1 cut(s) 1420
Bme18I GGWCC 4 cut(s) 110, 155, 1085, 1457
BmeRI GACNNNNNGTC 1 cut(s) 1083
BmgT120I GGNCC 5 cut(s) 110, 155, 1085, 1357, 1457
BmiI GGNNCC 2 cut(s) 941, 985
BmrFI CCNGG 1 cut(s) 1420
BmsI GCATC 4 cut(s) 334, 508, 518, 712
BpmI CTGGAG 1 cut(s) 990
BsaJI CCNNGG 1 cut(s) 138
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 44, 1103
Bse1I ACTGG 2 cut(s) 132, 237
Bse3DI GCAATG 1 cut(s) 813
BseBI CCWGG 1 cut(s) 1420
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 4 cut(s) 397, 655, 1239, 1256
BseLI CCNNNNNNNGG 3 cut(s) 43, 44, 1103
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 3 cut(s) 770, 912, 1255
BseNI ACTGG 2 cut(s) 132, 237
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 748
BseXI GCAGC 5 cut(s) 250, 253, 1087, 1303, 1415
Bsh1236I CGCG 1 cut(s) 945
BshFI GGCC 1 cut(s) 1359
BshNI GGYRCC 2 cut(s) 939, 983
BsiHKAI GWGCWC 1 cut(s) 748
BsiSI CCGG 1 cut(s) 1455
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 3 cut(s) 43, 44, 1103
BsmAI GTCTC 1 cut(s) 901
BsmFI GGGAC 1 cut(s) 330
BsmI GAATGC 2 cut(s) 1198, 1211
BsnI GGCC 1 cut(s) 1359
Bsp1286I GDGCHC 2 cut(s) 138, 748
Bsp143I GATC 2 cut(s) 33, 1093
BspACI CCGC 3 cut(s) 821, 943, 1325
BspANI GGCC 1 cut(s) 1359
BspCNI CTCAG 3 cut(s) 769, 911, 1254
BspFNI CGCG 1 cut(s) 945
BspLI GGNNCC 2 cut(s) 941, 985
BspMAI CTGCAG 1 cut(s) 1105
BspPI GGATC 2 cut(s) 28, 1088
BspQI GCTCTTC 2 cut(s) 126, 606
BspT107I GGYRCC 2 cut(s) 939, 983
BsrBI CCGCTC 1 cut(s) 821
BsrDI GCAATG 1 cut(s) 813
BsrI ACTGG 2 cut(s) 132, 237
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 2 cut(s) 33, 1093
BssT1I CCWWGG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 1420
Bst4CI ACNGT 3 cut(s) 155, 163, 926
Bst6I CTCTTC 5 cut(s) 21, 126, 276, 606, 1288
BstC8I GCNNGC 4 cut(s) 489, 804, 1347, 1452
BstDEI CTNAG 6 cut(s) 96, 293, 338, 756, 898, 1241
BstENI CCTNNNNNAGG 1 cut(s) 1101
BstF5I GGATG 4 cut(s) 397, 655, 1239, 1256
BstFNI CGCG 1 cut(s) 945
BstH2I RGCGCY 1 cut(s) 370
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 2 cut(s) 36, 1096
BstMAI GTCTC 1 cut(s) 901
BstMBI GATC 2 cut(s) 33, 1093
BstMWI GCNNNNNNNGC 2 cut(s) 1325, 1331
BstNI CCWGG 1 cut(s) 1420
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 1418
BstSFI CTRYAG 1 cut(s) 1101
BstSLI GKGCMC 1 cut(s) 748
BstUI CGCG 1 cut(s) 945
BstV1I GCAGC 5 cut(s) 250, 253, 1087, 1303, 1415
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 1 cut(s) 1359
BtgZI GCGATG 1 cut(s) 1179
BtsCI GGATG 4 cut(s) 397, 655, 1239, 1256
BtsIMutI CAGTG 2 cut(s) 789, 1146
Cac8I GCNNGC 4 cut(s) 489, 804, 1347, 1452
CaiI CAGNNNCTG 1 cut(s) 127
CfoI GCGC 1 cut(s) 369
Cfr13I GGNCC 5 cut(s) 110, 155, 1085, 1357, 1457
CpoI CGGWCCG 1 cut(s) 155
Csp6I GTAC 3 cut(s) 587, 940, 1370
CspI CGGWCCG 1 cut(s) 155
CviAII CATG 6 cut(s) 320, 727, 734, 1208, 1217, 1447
CviQI GTAC 3 cut(s) 587, 940, 1370
DdeI CTNAG 6 cut(s) 96, 293, 338, 756, 898, 1241
DpnI GATC 2 cut(s) 35, 1095
DpnII GATC 2 cut(s) 33, 1093
DrdI GACNNNNNNGTC 1 cut(s) 1064
DriI GACNNNNNGTC 1 cut(s) 1083
DseDI GACNNNNNNGTC 1 cut(s) 1064
Eam1104I CTCTTC 5 cut(s) 21, 126, 276, 606, 1288
Eam1105I GACNNNNNGTC 1 cut(s) 1083
EarI CTCTTC 5 cut(s) 21, 126, 276, 606, 1288
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 4 cut(s) 110, 155, 1085, 1457
Eco57I CTGAAG 3 cut(s) 300, 534, 776
EcoNI CCTNNNNNAGG 1 cut(s) 1101
EcoO109I RGGNCCY 2 cut(s) 110, 1357
EcoRII CCWGG 1 cut(s) 1418
EcoT14I CCWWGG 1 cut(s) 138
EcoT22I ATGCAT 4 cut(s) 349, 705, 776, 1213
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 6 cut(s) 323, 730, 737, 1211, 1220, 1450
FaqI GGGAC 1 cut(s) 330
FatI CATG 6 cut(s) 319, 726, 733, 1207, 1216, 1446
FblI GTMKAC 1 cut(s) 1122
Fnu4HI GCNGC 6 cut(s) 264, 267, 1101, 1292, 1326, 1404
FokI GGATG 4 cut(s) 404, 662, 1246, 1263
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 6 cut(s) 264, 267, 1101, 1292, 1326, 1404
FspBI CTAG 5 cut(s) 492, 662, 684, 837, 869
GlaI GCGC 1 cut(s) 368
GluI GCNGC 6 cut(s) 264, 267, 1101, 1292, 1326, 1404
GsuI CTGGAG 1 cut(s) 990
HaeII RGCGCY 1 cut(s) 370
HaeIII GGCC 1 cut(s) 1359
HapII CCGG 1 cut(s) 1455
HhaI GCGC 1 cut(s) 369
Hin1II CATG 6 cut(s) 323, 730, 737, 1211, 1220, 1450
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HincII GTYRAC 2 cut(s) 438, 535
HindII GTYRAC 2 cut(s) 438, 535
HindIII AAGCTT 5 cut(s) 290, 335, 420, 633, 788
HinfI GANTC 6 cut(s) 439, 460, 885, 1029, 1065, 1107
HpaI GTTAAC 1 cut(s) 535
HpaII CCGG 1 cut(s) 1455
HphI GGTGA 1 cut(s) 1322
Hpy166II GTNNAC 4 cut(s) 438, 535, 746, 1123
Hpy188III TCNNGA 7 cut(s) 227, 464, 608, 677, 1007, 1266, 1433
Hpy8I GTNNAC 4 cut(s) 438, 535, 746, 1123
HpyAV CCTTC 3 cut(s) 443, 1324, 1348
HpyCH4III ACNGT 3 cut(s) 155, 163, 926
HpyCH4IV ACGT 1 cut(s) 214
HpyF10VI GCNNNNNNNGC 2 cut(s) 1325, 1331
HpyF3I CTNAG 6 cut(s) 96, 293, 338, 756, 898, 1241
HpySE526I ACGT 1 cut(s) 214
Hsp92II CATG 6 cut(s) 323, 730, 737, 1211, 1220, 1450
HspAI GCGC 1 cut(s) 367
KpnI GGTACC 1 cut(s) 943
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 2 cut(s) 33, 1093
LguI GCTCTTC 2 cut(s) 126, 606
LmnI GCTCC 3 cut(s) 730, 913, 1009
Lsp1109I GCAGC 5 cut(s) 250, 253, 1087, 1303, 1415
LweI GCATC 4 cut(s) 334, 508, 518, 712
MaeI CTAG 5 cut(s) 492, 662, 684, 837, 869
MaeII ACGT 1 cut(s) 214
MaeIII GTNAC 2 cut(s) 149, 227
MalI GATC 2 cut(s) 35, 1095
MbiI CCGCTC 1 cut(s) 821
MboI GATC 2 cut(s) 33, 1093
MboII GAAGA 8 cut(s) 38, 143, 293, 343, 527, 623, 1237, 1275
MfeI CAATTG 1 cut(s) 39
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 2 cut(s) 138, 748
MlyI GAGTC 2 cut(s) 433, 1074
MmeI TCCRAC 3 cut(s) 182, 913, 1003
Mph1103I ATGCAT 4 cut(s) 349, 705, 776, 1213
MroXI GAANNNNTTC 1 cut(s) 459
MseI TTAA 5 cut(s) 432, 534, 693, 698, 1155
MslI CAYNNNNRTG 1 cut(s) 1073
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 1 cut(s) 1455
MspR9I CCNGG 1 cut(s) 1420
MunI CAATTG 1 cut(s) 39
Mva1269I GAATGC 2 cut(s) 1198, 1211
MvaI CCWGG 1 cut(s) 1420
MvnI CGCG 1 cut(s) 945
MwoI GCNNNNNNNGC 2 cut(s) 1325, 1331
NdeII GATC 2 cut(s) 33, 1093
NlaIII CATG 6 cut(s) 323, 730, 737, 1211, 1220, 1450
NlaIV GGNNCC 2 cut(s) 941, 985
NmuCI GTSAC 2 cut(s) 149, 227
NsiI ATGCAT 4 cut(s) 349, 705, 776, 1213
NspI RCATGY 1 cut(s) 323
PciI ACATGT 1 cut(s) 319
PciSI GCTCTTC 2 cut(s) 126, 606
PcsI WCGNNNNNNNCGW 1 cut(s) 748
PctI GAATGC 2 cut(s) 1198, 1211
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 4 cut(s) 460, 885, 1029, 1107
PkrI GCNGC 6 cut(s) 265, 268, 1102, 1293, 1327, 1405
PleI GAGTC 2 cut(s) 433, 1073
PpsI GAGTC 2 cut(s) 433, 1073
PpuMI RGGWCCY 1 cut(s) 110
PscI ACATGT 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 1418
PspGI CCWGG 1 cut(s) 1418
PspN4I GGNNCC 2 cut(s) 941, 985
PspPI GGNCC 5 cut(s) 110, 155, 1085, 1357, 1457
PspPPI RGGWCCY 1 cut(s) 110
PstI CTGCAG 1 cut(s) 1105
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 3 cut(s) 588, 941, 1371
RsaNI GTAC 3 cut(s) 587, 940, 1370
RseI CAYNNNNRTG 1 cut(s) 1073
Rsr2I CGGWCCG 1 cut(s) 155
RsrII CGGWCCG 1 cut(s) 155
SapI GCTCTTC 2 cut(s) 126, 606
SaqAI TTAA 5 cut(s) 432, 534, 693, 698, 1155
SatI GCNGC 6 cut(s) 264, 267, 1101, 1292, 1326, 1404
Sau3AI GATC 2 cut(s) 33, 1093
Sau96I GGNCC 5 cut(s) 110, 155, 1085, 1357, 1457
SchI GAGTC 2 cut(s) 433, 1074
ScrFI CCNGG 1 cut(s) 1420
SduI GDGCHC 2 cut(s) 138, 748
SfaNI GCATC 4 cut(s) 334, 508, 518, 712
SfcI CTRYAG 1 cut(s) 1101
SinI GGWCC 4 cut(s) 110, 155, 1085, 1457
SmiMI CAYNNNNRTG 1 cut(s) 1073
SpeI ACTAGT 1 cut(s) 661
SsiI CCGC 3 cut(s) 821, 943, 1325
SspMI CTAG 5 cut(s) 492, 662, 684, 837, 869
StyD4I CCNGG 1 cut(s) 1418
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 3 cut(s) 155, 163, 926
TaiI ACGT 1 cut(s) 217
TaqI TCGA 1 cut(s) 1265
TatI WGTACW 2 cut(s) 586, 1369
TauI GCSGC 1 cut(s) 1328
TfiI GAWTC 4 cut(s) 460, 885, 1029, 1107
Tru1I TTAA 5 cut(s) 432, 534, 693, 698, 1155
Tru9I TTAA 5 cut(s) 432, 534, 693, 698, 1155
TscAI CASTG 2 cut(s) 789, 1153
TseFI GTSAC 2 cut(s) 149, 227
TseI GCWGC 5 cut(s) 263, 266, 1100, 1291, 1403
Tsp45I GTSAC 2 cut(s) 149, 227
TspDTI ATGAA 7 cut(s) 67, 824, 939, 984, 1190, 1196, 1250
TspRI CASTG 2 cut(s) 789, 1153
VneI GTGCAC 1 cut(s) 744
VpaK11BI GGWCC 4 cut(s) 110, 155, 1085, 1457
XagI CCTNNNNNAGG 1 cut(s) 1101
XapI RAATTY 2 cut(s) 242, 1151
XceI RCATGY 1 cut(s) 323
XmiI GTMKAC 1 cut(s) 1122
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 5 cut(s) 492, 662, 684, 837, 869
Zsp2I ATGCAT 4 cut(s) 349, 705, 776, 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.