FvH4_4g04680

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013477
Physical Location & Seq
Forward (+)
4079744 .. 4082152
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04680.t1

Sequence Viewer

Length: 1560 bp
ATGTCGATCCTGGCCCCATTCCAGCTCCTGGAACTCAATGTGATATCGGCGCAGGACCTTGCACCGGTATCACGTTCCATGCGGACGTACGCCATTGCATGGGTGCACCCGGATCGCAAGTTGTCGACCCGGGTGGACACGCACGGCCACAACAACCCTACATGGAACGACAAGTTCGTGTTCCGGGTCGACGAGGACTTCTTGCATGAAGACACCTCTGCCGTGATGATCGAGATCTACGCGCTCCACTGGTTCAAAGACGTCCACGTCGGCACCATCCGTGTCCTCGTCGGCAATCTCATCCAAACTCCGGCGAAGCCCCACCACCACCACCCCCAGCTAGGCATGCGCTTCGTCGCATTACAGGTCCGGCGGCCCTCCGGGCGGCCACAGGGGATATTGAACATCGGGGTTGCCCTTCTTCATAGCTCCATGAGAAGCATGCCTTTGTATTCTCAGCTCAACTCATCCGCCGTGGGATATAAACAACTGATGGGAGAGGACGACCCGCCGAGGAGTCAAACCCAACAGTCAACGTTTTCGAAGCCGGAGCTGAGGCGGAGCAAGAGTGATTGCAGTTCCATGTTGGGGTCGGACCTCCGGTCCAAGGAGTTTAACAAGAAGGACAAAAAGGGAAGTTCAAAATCAAGCTCGGTGGTCAGCGGGTCGGAGGTCAGCGGTAGGAAGTATAAGAAGGGCCGATCCAAGGCCAGCTCGTTAATCAACGGCTCAGAACTCATCAAGGGCAGGGGTAGAAAAGGAAAAGCAAGCTCCGTGGTCAGCGCGTCTGACGTCAGCTGGAAGGGCAAAAAGGGTAAAGCGAGCTCCATTCTCAGCGTCTCCGACTTGGCCCCGAAAATTGGGGGGAAAGAGAAAAAGGGGAAGGCGAGCTCTTTGCTCAGCGGGTCGGATCCGAAACCGAACACCGGATCCGAACCCGACGAGTCGGTTAGAAAGCACAAGAACAACGGGAAATACAAAGTGCACGACATCGTGGCGGCGTGGCCGTTATCGAAGCCGTCGCCGAAGTACGTGGAGAGTTACGGAACTACCCCTGCGCGGAGGTCGTCGGTTCCGGGAAAGTCGCCGTTTAAGATGGGGACGTACGAGACGCCGCGGAAGTCGAATCTGATGGCGGTGCCGTATTTGACGGAGTCGGAGCTGGGGCCGTCGCCGTCGGAGGTGGCGGCGGCGATAGCGAGGGAGAGGGTGGACCAGGACAACGAGAGCTCGGTGGTGGTGGGGGCGTGGAACGAGGACGAGAGCGTGGAGGGGCTGCAGTCGAAGCTGGAGCGGTGGCGGACGGAGCTGCCGCCGGTGTACGACCGCGGCGACTATTCAAGTTTGCCGAGCAGCAGCGAGGGGCGACACACGCGGCGGCACACAGACGGCGGGAACGGGCTGTTCTCGTGCTTCAGTAACATCTGCGGCGTGGAGTGCTCCATCGTGTGCGGCGGCTCCGGCGAGGCGAGTAAGGAGAGGAAGAAGGCGGGTAGCAGCAGCAAGAGTAGCGGGCGGGTCAGGAAAAGCCCATCAGCGGATGATATGAGCTATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

520

Amino Acids

56.63

Weight (kDa)

9.71

Isoelectric Point (pI)

54.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 9 - 108 3.2e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04540 AT2G33320
fragaria_vesca FvH4_4g04680
malus_domestica MD13G1222100.v1.1 MD16G1227000.v1.1
prunus_persica Prupe.1G049100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0395501
rosa_laevigata RLG00000009618
rosa_multiflora Rmu_sc0003334.1_g000002 Rmu_sc0020819.1_g000001
rosa_roxburghii Rroxscaffold_5G00340430
rosa_rugosa Rorug03G0355700
rosa_samantha Rh4AG060600 Rh4AG061800 Rh4BG059600 Rh4CG065300 Rh4DG055500
rosa_wichuraiana Rw4G005050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
AatII GACGTC 2 cut(s) 264, 795
AccB1I GGYRCC 2 cut(s) 272, 1138
AccB7I CCANNNNNTGG 2 cut(s) 28, 99
AccBSI CCGCTC 1 cut(s) 1294
AccI GTMKAC 2 cut(s) 125, 189
AccII CGCG 6 cut(s) 242, 785, 1060, 1117, 1329, 1375
AclI AACGTT 1 cut(s) 536
AclWI GGATC 6 cut(s) 120, 696, 905, 918, 924, 937
AcoI YGGCCR 3 cut(s) 145, 386, 1004
AcuI CTGAAG 1 cut(s) 1399
AcyI GRCGYC 3 cut(s) 261, 792, 1112
AfaI GTAC 4 cut(s) 89, 1031, 1106, 1322
AgeI ACCGGT 1 cut(s) 64
AgsI TTSAA 4 cut(s) 256, 403, 642, 1341
AhdI GACNNNNNGTC 1 cut(s) 601
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 3 cut(s) 9, 27, 1215
AloI GAACNNNNNNTCC 2 cut(s) 914, 946
Alw21I GWGCWC 6 cut(s) 108, 827, 893, 987, 1232, 1442
Alw26I GTCTC 2 cut(s) 844, 1103
Alw44I GTGCAC 2 cut(s) 104, 983
AlwI GGATC 6 cut(s) 120, 696, 905, 918, 924, 937
AlwNI CAGNNNCTG 1 cut(s) 28
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 9 cut(s) 12, 145, 374, 386, 697, 708, 849, 1004, 1166
ApaLI GTGCAC 2 cut(s) 104, 983
ApeKI GCWGC 6 cut(s) 1276, 1309, 1353, 1356, 1497, 1500
AsiGI ACCGGT 1 cut(s) 64
AspLEI GCGC 5 cut(s) 52, 244, 351, 785, 1060
AsuC2I CCSGG 6 cut(s) 110, 130, 131, 185, 382, 1077
AsuII TTCGAA 1 cut(s) 542
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 5 cut(s) 55, 367, 595, 603, 1213
BaeGI GKGCMC 2 cut(s) 108, 987
BamHI GGATCC 2 cut(s) 910, 929
BanI GGYRCC 2 cut(s) 272, 1138
BanII GRGCYC 3 cut(s) 827, 893, 1232
BauI CACGAG 1 cut(s) 1408
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 6 cut(s) 108, 827, 893, 987, 1232, 1442
BbvCI CCTCAGC 1 cut(s) 554
BbvI GCAGC 6 cut(s) 1263, 1296, 1365, 1368, 1509, 1512
BccI CCATC 6 cut(s) 284, 487, 1090, 1126, 1451, 1540
BcgI CGANNNNNNTGC 6 cut(s) 95, 129, 334, 368, 877, 911
BciT130I CCWGG 3 cut(s) 11, 29, 1217
BcnI CCSGG 6 cut(s) 110, 130, 131, 185, 382, 1077
BcoDI GTCTC 2 cut(s) 844, 1103
BfaI CTAG 1 cut(s) 341
BfmI CTRYAG 1 cut(s) 1277
BglI GCCNNNNNGGC 1 cut(s) 382
BglII AGATCT 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 899
Bme1390I CCNGG 9 cut(s) 11, 29, 110, 130, 131, 185, 382, 1077, 1217
Bme18I GGWCC 5 cut(s) 55, 367, 595, 603, 1213
BmeRI GACNNNNNGTC 1 cut(s) 601
BmeT110I CYCGRG 1 cut(s) 129
BmgBI CACGTC 1 cut(s) 268
BmiI GGNNCC 9 cut(s) 15, 274, 852, 912, 931, 1074, 1140, 1167, 1459
BmrFI CCNGG 9 cut(s) 11, 29, 110, 130, 131, 185, 382, 1077, 1217
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 1310
Bpu10I CCTNAGC 1 cut(s) 554
Bpu1102I GCTNAGC 1 cut(s) 899
Bpu14I TTCGAA 1 cut(s) 542
BpuMI CCSGG 6 cut(s) 110, 130, 131, 185, 382, 1077
BsaAI YACGTR 1 cut(s) 1033
BsaBI GATNNNNATC 1 cut(s) 233
BsaHI GRCGYC 3 cut(s) 261, 792, 1112
BsaJI CCNNGG 8 cut(s) 129, 474, 512, 606, 705, 774, 1115, 1327
BsaWI WCCGGW 3 cut(s) 64, 600, 926
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bse118I RCCGGY 2 cut(s) 64, 1315
Bse1I ACTGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 233
BseBI CCWGG 3 cut(s) 11, 29, 1217
BseDI CCNNGG 8 cut(s) 129, 474, 512, 606, 705, 774, 1115, 1327
BseGI GGATG 4 cut(s) 276, 300, 467, 1546
BseJI GATNNNNATC 1 cut(s) 233
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 5 cut(s) 470, 545, 744, 847, 913
BseNI ACTGG 1 cut(s) 254
BseRI GAGGAG 1 cut(s) 529
BseSI GKGCMC 2 cut(s) 108, 987
BseXI GCAGC 6 cut(s) 1263, 1296, 1365, 1368, 1509, 1512
BseYI CCCAGC 2 cut(s) 336, 1162
Bsh1236I CGCG 6 cut(s) 242, 785, 1060, 1117, 1329, 1375
Bsh1285I CGRYCG 1 cut(s) 1327
BshFI GGCC 9 cut(s) 14, 147, 376, 388, 699, 710, 851, 1006, 1168
BshNI GGYRCC 2 cut(s) 272, 1138
BshTI ACCGGT 1 cut(s) 64
BsiEI CGRYCG 1 cut(s) 1327
BsiHKAI GWGCWC 6 cut(s) 108, 827, 893, 987, 1232, 1442
BsiHKCI CYCGRG 1 cut(s) 129
BsiWI CGTACG 2 cut(s) 87, 1104
BslFI GGGAC 1 cut(s) 1114
BsmAI GTCTC 2 cut(s) 844, 1103
BsmBI CGTCTC 2 cut(s) 844, 1103
BsmFI GGGAC 1 cut(s) 1114
BsnI GGCC 9 cut(s) 14, 147, 376, 388, 699, 710, 851, 1006, 1168
BsoBI CYCGRG 1 cut(s) 129
Bsp119I TTCGAA 1 cut(s) 542
Bsp1286I GDGCHC 6 cut(s) 108, 827, 893, 987, 1232, 1442
Bsp143I GATC 7 cut(s) 6, 112, 228, 234, 701, 910, 929
Bsp1720I GCTNAGC 1 cut(s) 899
BspANI GGCC 9 cut(s) 14, 147, 376, 388, 699, 710, 851, 1006, 1168
BspCNI CTCAG 5 cut(s) 469, 546, 743, 846, 912
BspFNI CGCG 6 cut(s) 242, 785, 1060, 1117, 1329, 1375
BspLI GGNNCC 9 cut(s) 15, 274, 852, 912, 931, 1074, 1140, 1167, 1459
BspMAI CTGCAG 1 cut(s) 1281
BspPI GGATC 6 cut(s) 120, 696, 905, 918, 924, 937
BspT104I TTCGAA 1 cut(s) 542
BspT107I GGYRCC 2 cut(s) 272, 1138
BsrBI CCGCTC 1 cut(s) 1294
BsrDI GCAATG 1 cut(s) 93
BsrFI RCCGGY 2 cut(s) 64, 1315
BsrI ACTGG 1 cut(s) 254
BssAI RCCGGY 2 cut(s) 64, 1315
BssECI CCNNGG 8 cut(s) 129, 474, 512, 606, 705, 774, 1115, 1327
BssMI GATC 7 cut(s) 6, 112, 228, 234, 701, 910, 929
BssNI GRCGYC 3 cut(s) 261, 792, 1112
BssSI CACGAG 1 cut(s) 1408
BssT1I CCWWGG 2 cut(s) 606, 705
Bst2BI CACGAG 1 cut(s) 1408
Bst2UI CCWGG 3 cut(s) 11, 29, 1217
Bst4CI ACNGT 1 cut(s) 531
BstACI GRCGYC 3 cut(s) 261, 792, 1112
BstBAI YACGTR 1 cut(s) 1033
BstBI TTCGAA 1 cut(s) 542
BstC8I GCNNGC 7 cut(s) 347, 443, 712, 769, 823, 889, 1514
BstDEI CTNAG 5 cut(s) 456, 554, 730, 833, 899
BstDSI CCRYGG 4 cut(s) 474, 774, 1115, 1327
BstF5I GGATG 4 cut(s) 276, 300, 467, 1546
BstFNI CGCG 6 cut(s) 242, 785, 1060, 1117, 1329, 1375
BstHHI GCGC 5 cut(s) 52, 244, 351, 785, 1060
BstKTI GATC 7 cut(s) 9, 115, 231, 237, 704, 913, 932
BstMAI GTCTC 2 cut(s) 844, 1103
BstMBI GATC 7 cut(s) 6, 112, 228, 234, 701, 910, 929
BstMCI CGRYCG 1 cut(s) 1327
BstNI CCWGG 3 cut(s) 11, 29, 1217
BstNSI RCATGY 2 cut(s) 349, 445
BstSCI CCNGG 9 cut(s) 9, 27, 108, 128, 129, 183, 380, 1075, 1215
BstSFI CTRYAG 1 cut(s) 1277
BstSLI GKGCMC 2 cut(s) 108, 987
BstUI CGCG 6 cut(s) 242, 785, 1060, 1117, 1329, 1375
BstV1I GCAGC 6 cut(s) 1263, 1296, 1365, 1368, 1509, 1512
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 3 cut(s) 234, 910, 929
BstYI RGATCY 3 cut(s) 234, 910, 929
BsuRI GGCC 9 cut(s) 14, 147, 376, 388, 699, 710, 851, 1006, 1168
BtgI CCRYGG 4 cut(s) 474, 774, 1115, 1327
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 4 cut(s) 276, 300, 467, 1546
BtsIMutI CAGTG 1 cut(s) 247
Cac8I GCNNGC 7 cut(s) 347, 443, 712, 769, 823, 889, 1514
CaiI CAGNNNCTG 1 cut(s) 28
CfoI GCGC 5 cut(s) 52, 244, 351, 785, 1060
Cfr10I RCCGGY 2 cut(s) 64, 1315
Cfr42I CCGCGG 2 cut(s) 1118, 1330
Cfr9I CCCGGG 1 cut(s) 129
CseI GACGC 3 cut(s) 774, 826, 1120
Csp6I GTAC 4 cut(s) 88, 1030, 1105, 1321
CspAI ACCGGT 1 cut(s) 64
CspCI CAANNNNNGTGG 4 cut(s) 636, 671, 756, 791
CviAII CATG 8 cut(s) 79, 99, 162, 206, 346, 433, 442, 583
CviQI GTAC 4 cut(s) 88, 1030, 1105, 1321
DdeI CTNAG 5 cut(s) 456, 554, 730, 833, 899
DpnI GATC 7 cut(s) 8, 114, 230, 236, 703, 912, 931
DpnII GATC 7 cut(s) 6, 112, 228, 234, 701, 910, 929
DrdI GACNNNNNNGTC 1 cut(s) 266
DriI GACNNNNNGTC 1 cut(s) 601
DseDI GACNNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 3 cut(s) 145, 386, 1004
Eam1105I GACNNNNNGTC 1 cut(s) 601
EciI GGCGGA 3 cut(s) 460, 574, 1315
Ecl136II GAGCTC 3 cut(s) 825, 891, 1230
Eco130I CCWWGG 2 cut(s) 606, 705
Eco24I GRGCYC 3 cut(s) 827, 893, 1232
Eco32I GATATC 1 cut(s) 45
Eco47I GGWCC 5 cut(s) 55, 367, 595, 603, 1213
Eco53kI GAGCTC 3 cut(s) 825, 891, 1230
Eco57I CTGAAG 1 cut(s) 1399
Eco88I CYCGRG 1 cut(s) 129
EcoICRI GAGCTC 3 cut(s) 825, 891, 1230
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 3 cut(s) 9, 27, 1215
EcoRV GATATC 1 cut(s) 45
EcoT14I CCWWGG 2 cut(s) 606, 705
EcoT38I GRGCYC 3 cut(s) 827, 893, 1232
ErhI CCWWGG 2 cut(s) 606, 705
Esp3I CGTCTC 2 cut(s) 844, 1103
FaeI CATG 8 cut(s) 82, 102, 165, 209, 349, 436, 445, 586
FalI AAGNNNNNCTT 2 cut(s) 430, 462
FaqI GGGAC 1 cut(s) 1114
FatI CATG 8 cut(s) 78, 98, 161, 205, 345, 432, 441, 582
FauI CCCGC 7 cut(s) 516, 656, 896, 1385, 1483, 1505, 1509
FblI GTMKAC 2 cut(s) 125, 189
FokI GGATG 4 cut(s) 263, 287, 454, 1553
FriOI GRGCYC 3 cut(s) 827, 893, 1232
FspBI CTAG 1 cut(s) 341
GlaI GCGC 5 cut(s) 51, 243, 350, 784, 1059
GsaI CCCAGC 2 cut(s) 340, 1166
GsuI CTGGAG 1 cut(s) 1310
HaeIII GGCC 9 cut(s) 14, 147, 376, 388, 699, 710, 851, 1006, 1168
HgaI GACGC 3 cut(s) 774, 826, 1120
HhaI GCGC 5 cut(s) 52, 244, 351, 785, 1060
Hin1I GRCGYC 3 cut(s) 261, 792, 1112
Hin1II CATG 8 cut(s) 82, 102, 165, 209, 349, 436, 445, 586
Hin6I GCGC 5 cut(s) 50, 242, 349, 783, 1058
HinP1I GCGC 5 cut(s) 50, 242, 349, 783, 1058
HincII GTYRAC 3 cut(s) 126, 190, 534
HindII GTYRAC 3 cut(s) 126, 190, 534
HinfI GANTC 4 cut(s) 517, 944, 1126, 1154
Hpy166II GTNNAC 9 cut(s) 106, 126, 136, 190, 265, 534, 985, 1213, 1321
Hpy188III TCNNGA 2 cut(s) 232, 1522
Hpy8I GTNNAC 9 cut(s) 106, 126, 136, 190, 265, 534, 985, 1213, 1321
Hpy99I CGWCG 9 cut(s) 194, 272, 293, 359, 944, 1024, 1072, 1174, 1180
HpyAV CCTTC 6 cut(s) 428, 616, 688, 796, 877, 1480
HpyCH4III ACNGT 1 cut(s) 531
HpyCH4IV ACGT 8 cut(s) 73, 86, 261, 267, 536, 792, 1032, 1103
HpyCH4V TGCA 7 cut(s) 62, 98, 106, 205, 576, 985, 1279
HpyF3I CTNAG 5 cut(s) 456, 554, 730, 833, 899
HpySE526I ACGT 8 cut(s) 73, 86, 261, 267, 536, 792, 1032, 1103
Hsp92I GRCGYC 3 cut(s) 261, 792, 1112
Hsp92II CATG 8 cut(s) 82, 102, 165, 209, 349, 436, 445, 586
HspAI GCGC 5 cut(s) 50, 242, 349, 783, 1058
KspI CCGCGG 2 cut(s) 1118, 1330
Kzo9I GATC 7 cut(s) 6, 112, 228, 234, 701, 910, 929
Lsp1109I GCAGC 6 cut(s) 1263, 1296, 1365, 1368, 1509, 1512
MaeI CTAG 1 cut(s) 341
MaeII ACGT 8 cut(s) 73, 86, 261, 267, 536, 792, 1032, 1103
MaeIII GTNAC 2 cut(s) 1040, 1418
MalI GATC 7 cut(s) 8, 114, 230, 236, 703, 912, 931
MbiI CCGCTC 1 cut(s) 1294
MboI GATC 7 cut(s) 6, 112, 228, 234, 701, 910, 929
MboII GAAGA 3 cut(s) 221, 413, 1495
MflI RGATCY 3 cut(s) 234, 910, 929
MhlI GDGCHC 6 cut(s) 108, 827, 893, 987, 1232, 1442
MluCI AATT 1 cut(s) 858
MlyI GAGTC 3 cut(s) 526, 953, 1163
MmeI TCCRAC 6 cut(s) 573, 648, 867, 888, 1137, 1158
MseI TTAA 3 cut(s) 615, 719, 1092
MspA1I CMGCKG 7 cut(s) 663, 678, 798, 903, 1117, 1329, 1538
MspR9I CCNGG 9 cut(s) 11, 29, 110, 130, 131, 185, 382, 1077, 1217
MvaI CCWGG 3 cut(s) 11, 29, 1217
MvnI CGCG 6 cut(s) 242, 785, 1060, 1117, 1329, 1375
NciI CCSGG 6 cut(s) 110, 130, 131, 185, 382, 1077
NdeII GATC 7 cut(s) 6, 112, 228, 234, 701, 910, 929
NlaIII CATG 8 cut(s) 82, 102, 165, 209, 349, 436, 445, 586
NlaIV GGNNCC 9 cut(s) 15, 274, 852, 912, 931, 1074, 1140, 1167, 1459
NmeAIII GCCGAG 2 cut(s) 537, 1374
NspI RCATGY 2 cut(s) 349, 445
NspV TTCGAA 1 cut(s) 542
PaeI GCATGC 2 cut(s) 349, 445
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 1329
PfeI GAWTC 1 cut(s) 1126
Pfl23II CGTACG 2 cut(s) 87, 1104
PflFI GACNNNGTC 1 cut(s) 1153
PflMI CCANNNNNTGG 2 cut(s) 28, 99
PfoI TCCNGGA 2 cut(s) 27, 1075
PinAI ACCGGT 1 cut(s) 64
PleI GAGTC 3 cut(s) 525, 952, 1162
PpsI GAGTC 3 cut(s) 525, 952, 1162
Ppu21I YACGTR 1 cut(s) 1033
PpuMI RGGWCCY 1 cut(s) 55
Psp124BI GAGCTC 3 cut(s) 827, 893, 1232
Psp1406I AACGTT 1 cut(s) 536
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 3 cut(s) 9, 27, 1215
PspFI CCCAGC 2 cut(s) 336, 1162
PspGI CCWGG 3 cut(s) 9, 27, 1215
PspLI CGTACG 2 cut(s) 87, 1104
PspN4I GGNNCC 9 cut(s) 15, 274, 852, 912, 931, 1074, 1140, 1167, 1459
PspPPI RGGWCCY 1 cut(s) 55
PstI CTGCAG 1 cut(s) 1281
PstNI CAGNNNCTG 1 cut(s) 28
PsuI RGATCY 3 cut(s) 234, 910, 929
PsyI GACNNNGTC 1 cut(s) 1153
PvuII CAGCTG 1 cut(s) 798
RsaI GTAC 4 cut(s) 89, 1031, 1106, 1322
RsaNI GTAC 4 cut(s) 88, 1030, 1105, 1321
SacI GAGCTC 3 cut(s) 827, 893, 1232
SacII CCGCGG 2 cut(s) 1118, 1330
SalI GTCGAC 2 cut(s) 124, 188
SaqAI TTAA 3 cut(s) 615, 719, 1092
Sau3AI GATC 7 cut(s) 6, 112, 228, 234, 701, 910, 929
SchI GAGTC 3 cut(s) 526, 953, 1163
ScrFI CCNGG 9 cut(s) 11, 29, 110, 130, 131, 185, 382, 1077, 1217
SduI GDGCHC 6 cut(s) 108, 827, 893, 987, 1232, 1442
SfcI CTRYAG 1 cut(s) 1277
Sfr303I CCGCGG 2 cut(s) 1118, 1330
SfuI TTCGAA 1 cut(s) 542
SgrAI CRCCGGYG 1 cut(s) 1315
SgrBI CCGCGG 2 cut(s) 1118, 1330
SinI GGWCC 5 cut(s) 55, 367, 595, 603, 1213
SmaI CCCGGG 1 cut(s) 131
SphI GCATGC 2 cut(s) 349, 445
Sse9I AATT 1 cut(s) 858
SspMI CTAG 1 cut(s) 341
SstI GAGCTC 3 cut(s) 827, 893, 1232
StyD4I CCNGG 9 cut(s) 9, 27, 108, 128, 129, 183, 380, 1075, 1215
StyI CCWWGG 2 cut(s) 606, 705
TaaI ACNGT 1 cut(s) 531
TaiI ACGT 8 cut(s) 76, 89, 264, 270, 539, 795, 1035, 1106
TaqI TCGA 8 cut(s) 5, 125, 189, 231, 542, 1013, 1124, 1283
TasI AATT 1 cut(s) 858
TfiI GAWTC 1 cut(s) 1126
Tru1I TTAA 3 cut(s) 615, 719, 1092
Tru9I TTAA 3 cut(s) 615, 719, 1092
TscAI CASTG 1 cut(s) 254
TseI GCWGC 6 cut(s) 1276, 1309, 1353, 1356, 1497, 1500
TspDTI ATGAA 2 cut(s) 222, 413
TspGWI ACGGA 5 cut(s) 269, 763, 1059, 1166, 1319
TspMI CCCGGG 1 cut(s) 129
TspRI CASTG 1 cut(s) 254
Tth111I GACNNNGTC 1 cut(s) 1153
Van91I CCANNNNNTGG 2 cut(s) 28, 99
VneI GTGCAC 2 cut(s) 104, 983
VpaK11BI GGWCC 5 cut(s) 55, 367, 595, 603, 1213
XceI RCATGY 2 cut(s) 349, 445
XmaI CCCGGG 1 cut(s) 129
XmiI GTMKAC 2 cut(s) 125, 189
XspI CTAG 1 cut(s) 341
ZraI GACGTC 2 cut(s) 262, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.