Rmu_sc0020819.1_g000001

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020819.1
OG0013477
Physical Location & Seq
Reverse (-)
2 .. 666
665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020819.1_g000001.1.cds

Sequence Viewer

Length: 665 bp
atgatcgagatctacgccctccactggttcaaagacgtccacgtcggtaccatccgtgtcctcgtcggcaatctcatccccactccggtgaagccccaccaccaccatcctcagctgggcatgcgcttcgtggcgctccaggtacggcggccctccggccggccacaggggatattgaacatcggtgtggcgctgcttcacagctccatgcgcagcatgcctttgtattctcagctcagcgcatccgccgtgggatataaacagttaatgggggaggacgacccgccgcggagtcaaacccaccggagtcagtcaacgttttcaaaagcggagctgagacgcagcaagagtgactgcagctccatgttggcgtcggagatccggaccaaggaatttacgaaaaaggacaaaaagagcagagggagctcggtggtcagcgggtccgacgtcagcagccaaaagaataagaagggccgatccaaggccagctcgttaatcaacggctcagaaatcatcaggggaaggggtagaaaagggaaagccagctccgtggtcagcgcgtcagaattaagccggaccaagggtaaaaacggaaaaccaagttccatactcagcgtctccgacgttggtggggccaaggacaaaaagggaaaaccaagttccat
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

221

Amino Acids

23.94

Weight (kDa)

10.75

Isoelectric Point (pI)

53.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04540 AT2G33320
fragaria_vesca FvH4_4g04680
malus_domestica MD13G1222100.v1.1 MD16G1227000.v1.1
prunus_persica Prupe.1G049100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0395501
rosa_laevigata RLG00000009618
rosa_multiflora Rmu_sc0003334.1_g000002 Rmu_sc0020819.1_g000001
rosa_roxburghii Rroxscaffold_5G00340430
rosa_rugosa Rorug03G0355700
rosa_samantha Rh4AG060600 Rh4AG061800 Rh4BG059600 Rh4CG065300 Rh4DG055500
rosa_wichuraiana Rw4G005050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 41
AatII GACGTC 2 cut(s) 39, 450
Acc16I TGCGCA 1 cut(s) 212
Acc65I GGTACC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 47
AccII CGCG 2 cut(s) 289, 560
AccIII TCCGGA 1 cut(s) 381
AciI CCGC 7 cut(s) 148, 246, 284, 287, 289, 329, 438
AclI AACGTT 1 cut(s) 317
AclWI GGATC 2 cut(s) 373, 471
AcoI YGGCCR 2 cut(s) 157, 161
AcsI RAATTY 1 cut(s) 392
AcyI GRCGYC 3 cut(s) 36, 371, 447
AfaI GTAC 2 cut(s) 49, 144
AfiI CCNNNNNNNGG 8 cut(s) 24, 85, 116, 159, 166, 388, 481, 580
AgsI TTSAA 3 cut(s) 31, 178, 324
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 1 cut(s) 138
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 8 cut(s) 115, 204, 235, 334, 360, 426, 489, 546
AluI AGCT 8 cut(s) 115, 204, 235, 334, 360, 426, 489, 546
Alw21I GWGCWC 1 cut(s) 428
Alw26I GTCTC 2 cut(s) 331, 622
AlwI GGATC 2 cut(s) 373, 471
Aor13HI TCCGGA 1 cut(s) 381
AoxI GGCC 6 cut(s) 149, 157, 161, 472, 483, 633
ApeKI GCWGC 5 cut(s) 193, 213, 342, 357, 453
ApoI RAATTY 1 cut(s) 392
Asp718I GGTACC 1 cut(s) 47
AspLEI GCGC 6 cut(s) 126, 136, 193, 213, 242, 560
AspS9I GGNCC 6 cut(s) 150, 384, 441, 472, 576, 633
AsuHPI GGTGA 1 cut(s) 100
AvaII GGWCC 3 cut(s) 384, 441, 576
BaeI ACNNNNGTAYC 2 cut(s) 31, 64
BanI GGYRCC 1 cut(s) 47
BanII GRGCYC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 428
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 5 cut(s) 180, 225, 354, 369, 465
BccI CCATC 2 cut(s) 59, 114
BceAI ACGGC 3 cut(s) 161, 233, 517
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 331, 622
BfmI CTRYAG 1 cut(s) 355
BfoI RGCGCY 2 cut(s) 137, 194
BglII AGATCT 1 cut(s) 9
BisI GCNGC 7 cut(s) 149, 194, 214, 287, 343, 358, 454
BlpI GCTNAGC 1 cut(s) 236
BlsI GCNGC 7 cut(s) 150, 195, 215, 288, 344, 359, 455
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 3 cut(s) 384, 441, 576
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 6 cut(s) 150, 384, 441, 472, 576, 633
BmiI GGNNCC 3 cut(s) 49, 442, 634
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 122
Bpu10I CCTNAGC 1 cut(s) 111
Bpu1102I GCTNAGC 1 cut(s) 236
BsaBI GATNNNNATC 1 cut(s) 8
BsaHI GRCGYC 3 cut(s) 36, 371, 447
BsaJI CCNNGG 7 cut(s) 249, 287, 387, 480, 549, 579, 636
BsaWI WCCGGW 3 cut(s) 85, 303, 381
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 8 cut(s) 24, 85, 116, 159, 166, 388, 481, 580
Bse118I RCCGGY 1 cut(s) 159
Bse1I ACTGG 1 cut(s) 29
Bse8I GATNNNNATC 1 cut(s) 8
BseAI TCCGGA 1 cut(s) 381
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 7 cut(s) 249, 287, 387, 480, 549, 579, 636
BseGI GGATG 4 cut(s) 51, 75, 106, 242
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 8 cut(s) 24, 85, 116, 159, 166, 388, 481, 580
BseMII CTCAG 6 cut(s) 125, 245, 250, 326, 519, 625
BseNI ACTGG 1 cut(s) 29
BseX3I CGGCCG 1 cut(s) 157
BseXI GCAGC 5 cut(s) 180, 225, 354, 369, 465
BseYI CCCAGC 1 cut(s) 115
Bsh1236I CGCG 2 cut(s) 289, 560
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 6 cut(s) 151, 159, 163, 474, 485, 635
BshNI GGYRCC 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 428
BsiSI CCGG 6 cut(s) 86, 156, 160, 304, 382, 574
BslI CCNNNNNNNGG 8 cut(s) 24, 85, 116, 159, 166, 388, 481, 580
BsmAI GTCTC 2 cut(s) 331, 622
BsmBI CGTCTC 2 cut(s) 331, 622
BsnI GGCC 6 cut(s) 151, 159, 163, 474, 485, 635
Bsp1286I GDGCHC 1 cut(s) 428
Bsp13I TCCGGA 1 cut(s) 381
Bsp143I GATC 4 cut(s) 3, 9, 378, 476
Bsp1720I GCTNAGC 1 cut(s) 236
BspACI CCGC 7 cut(s) 148, 246, 284, 287, 289, 329, 438
BspANI GGCC 6 cut(s) 151, 159, 163, 474, 485, 635
BspCNI CTCAG 6 cut(s) 124, 244, 249, 327, 518, 624
BspEI TCCGGA 1 cut(s) 381
BspFNI CGCG 2 cut(s) 289, 560
BspLI GGNNCC 3 cut(s) 49, 442, 634
BspMAI CTGCAG 1 cut(s) 359
BspPI GGATC 2 cut(s) 373, 471
BspT107I GGYRCC 1 cut(s) 47
BsrFI RCCGGY 1 cut(s) 159
BsrI ACTGG 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 7 cut(s) 249, 287, 387, 480, 549, 579, 636
BssMI GATC 4 cut(s) 3, 9, 378, 476
BssNI GRCGYC 3 cut(s) 36, 371, 447
BssT1I CCWWGG 4 cut(s) 387, 480, 579, 636
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 264
BstACI GRCGYC 3 cut(s) 36, 371, 447
BstC8I GCNNGC 5 cut(s) 122, 161, 218, 487, 544
BstDEI CTNAG 6 cut(s) 111, 231, 236, 335, 505, 611
BstDSI CCRYGG 3 cut(s) 249, 287, 549
BstF5I GGATG 4 cut(s) 51, 75, 106, 242
BstFNI CGCG 2 cut(s) 289, 560
BstH2I RGCGCY 2 cut(s) 137, 194
BstHHI GCGC 6 cut(s) 126, 136, 193, 213, 242, 560
BstKTI GATC 4 cut(s) 6, 12, 381, 479
BstMAI GTCTC 2 cut(s) 331, 622
BstMBI GATC 4 cut(s) 3, 9, 378, 476
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 4 cut(s) 121, 210, 217, 423
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 2 cut(s) 124, 220
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 355
BstUI CGCG 2 cut(s) 289, 560
BstV1I GCAGC 5 cut(s) 180, 225, 354, 369, 465
BstX2I RGATCY 2 cut(s) 9, 378
BstXI CCANNNNNNTGG 1 cut(s) 550
BstYI RGATCY 2 cut(s) 9, 378
BstZI CGGCCG 1 cut(s) 157
BsuRI GGCC 6 cut(s) 151, 159, 163, 474, 485, 635
BtgI CCRYGG 3 cut(s) 249, 287, 549
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 4 cut(s) 51, 75, 106, 242
BtsIMutI CAGTG 1 cut(s) 22
Cac8I GCNNGC 5 cut(s) 122, 161, 218, 487, 544
CfoI GCGC 6 cut(s) 126, 136, 193, 213, 242, 560
Cfr10I RCCGGY 1 cut(s) 159
Cfr13I GGNCC 6 cut(s) 150, 384, 441, 472, 576, 633
Cfr42I CCGCGG 1 cut(s) 290
CseI GACGC 4 cut(s) 348, 360, 549, 604
Csp6I GTAC 2 cut(s) 48, 143
CviAII CATG 4 cut(s) 121, 208, 217, 364
CviQI GTAC 2 cut(s) 48, 143
DdeI CTNAG 6 cut(s) 111, 231, 236, 335, 505, 611
DpnI GATC 4 cut(s) 5, 11, 380, 478
DpnII GATC 4 cut(s) 3, 9, 378, 476
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 2 cut(s) 157, 161
EagI CGGCCG 1 cut(s) 157
EciI GGCGGA 1 cut(s) 235
Ecl136II GAGCTC 1 cut(s) 426
EclXI CGGCCG 1 cut(s) 157
Eco130I CCWWGG 4 cut(s) 387, 480, 579, 636
Eco24I GRGCYC 1 cut(s) 428
Eco47I GGWCC 3 cut(s) 384, 441, 576
Eco52I CGGCCG 1 cut(s) 157
Eco53kI GAGCTC 1 cut(s) 426
EcoICRI GAGCTC 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 4 cut(s) 387, 480, 579, 636
EcoT38I GRGCYC 1 cut(s) 428
ErhI CCWWGG 4 cut(s) 387, 480, 579, 636
Esp3I CGTCTC 2 cut(s) 331, 622
FaeI CATG 4 cut(s) 124, 211, 220, 367
FaiI YATR 6 cut(s) 122, 209, 218, 258, 365, 608
FatI CATG 4 cut(s) 120, 207, 216, 363
FauI CCCGC 2 cut(s) 291, 431
Fnu4HI GCNGC 7 cut(s) 149, 194, 214, 287, 343, 358, 454
FokI GGATG 4 cut(s) 38, 62, 93, 229
FriOI GRGCYC 1 cut(s) 428
FseI GGCCGGCC 1 cut(s) 163
Fsp4HI GCNGC 7 cut(s) 149, 194, 214, 287, 343, 358, 454
FspI TGCGCA 1 cut(s) 212
GlaI GCGC 6 cut(s) 125, 135, 192, 212, 241, 559
GluI GCNGC 7 cut(s) 149, 194, 214, 287, 343, 358, 454
GsaI CCCAGC 1 cut(s) 119
GsuI CTGGAG 1 cut(s) 122
HaeII RGCGCY 2 cut(s) 137, 194
HaeIII GGCC 6 cut(s) 151, 159, 163, 474, 485, 635
HapII CCGG 6 cut(s) 86, 156, 160, 304, 382, 574
HgaI GACGC 4 cut(s) 348, 360, 549, 604
HhaI GCGC 6 cut(s) 126, 136, 193, 213, 242, 560
Hin1I GRCGYC 3 cut(s) 36, 371, 447
Hin1II CATG 4 cut(s) 124, 211, 220, 367
Hin6I GCGC 6 cut(s) 124, 134, 191, 211, 240, 558
HinP1I GCGC 6 cut(s) 124, 134, 191, 211, 240, 558
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HinfI GANTC 2 cut(s) 292, 307
HpaII CCGG 6 cut(s) 86, 156, 160, 304, 382, 574
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 2 cut(s) 40, 315
Hpy188I TCNGA 5 cut(s) 376, 445, 508, 565, 622
Hpy188III TCNNGA 2 cut(s) 7, 382
Hpy8I GTNNAC 2 cut(s) 40, 315
Hpy99I CGWCG 5 cut(s) 47, 68, 376, 449, 626
HpyAV CCTTC 2 cut(s) 463, 516
HpyCH4III ACNGT 1 cut(s) 264
HpyCH4IV ACGT 5 cut(s) 36, 42, 317, 447, 624
HpyCH4V TGCA 1 cut(s) 357
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 210, 217, 423
HpyF3I CTNAG 6 cut(s) 111, 231, 236, 335, 505, 611
HpySE526I ACGT 5 cut(s) 36, 42, 317, 447, 624
Hsp92I GRCGYC 3 cut(s) 36, 371, 447
Hsp92II CATG 4 cut(s) 124, 211, 220, 367
HspAI GCGC 6 cut(s) 124, 134, 191, 211, 240, 558
Kpn2I TCCGGA 1 cut(s) 381
KpnI GGTACC 1 cut(s) 51
KroI GCCGGC 1 cut(s) 159
KroNI GCCGGC 1 cut(s) 161
KspI CCGCGG 1 cut(s) 290
Kzo9I GATC 4 cut(s) 3, 9, 378, 476
LmnI GCTCC 6 cut(s) 141, 209, 331, 365, 423, 551
Lsp1109I GCAGC 5 cut(s) 180, 225, 354, 369, 465
LweI GCATC 1 cut(s) 251
MaeII ACGT 5 cut(s) 36, 42, 317, 447, 624
MaeIII GTNAC 1 cut(s) 350
MalI GATC 4 cut(s) 5, 11, 380, 478
MboI GATC 4 cut(s) 3, 9, 378, 476
MflI RGATCY 2 cut(s) 9, 378
MhlI GDGCHC 1 cut(s) 428
MluCI AATT 2 cut(s) 392, 566
MlyI GAGTC 2 cut(s) 301, 316
MmeI TCCRAC 3 cut(s) 354, 468, 645
MnlI CCTC 6 cut(s) 29, 71, 120, 163, 268, 413
MroI TCCGGA 1 cut(s) 381
MroNI GCCGGC 1 cut(s) 159
MseI TTAA 3 cut(s) 266, 494, 569
MslI CAYNNNNRTG 2 cut(s) 86, 185
MspA1I CMGCKG 3 cut(s) 115, 289, 438
MspI CCGG 6 cut(s) 86, 156, 160, 304, 382, 574
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 2 cut(s) 289, 560
MwoI GCNNNNNNNGC 4 cut(s) 121, 210, 217, 423
NaeI GCCGGC 1 cut(s) 161
NdeII GATC 4 cut(s) 3, 9, 378, 476
NgoMIV GCCGGC 1 cut(s) 159
NlaIII CATG 4 cut(s) 124, 211, 220, 367
NlaIV GGNNCC 3 cut(s) 49, 442, 634
NmuCI GTSAC 1 cut(s) 350
NsbI TGCGCA 1 cut(s) 212
NspI RCATGY 2 cut(s) 124, 220
OliI CACNNNNGTG 1 cut(s) 86
PaeI GCATGC 2 cut(s) 124, 220
PdiI GCCGGC 1 cut(s) 161
PkrI GCNGC 7 cut(s) 150, 195, 215, 288, 344, 359, 455
PleI GAGTC 2 cut(s) 300, 315
PpsI GAGTC 2 cut(s) 300, 315
Psp124BI GAGCTC 1 cut(s) 428
Psp1406I AACGTT 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 138
PspFI CCCAGC 1 cut(s) 115
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 49, 442, 634
PspPI GGNCC 6 cut(s) 150, 384, 441, 472, 576, 633
PstI CTGCAG 1 cut(s) 359
PsuI RGATCY 2 cut(s) 9, 378
PvuII CAGCTG 1 cut(s) 115
RigI GGCCGGCC 1 cut(s) 163
RsaI GTAC 2 cut(s) 49, 144
RsaNI GTAC 2 cut(s) 48, 143
RseI CAYNNNNRTG 2 cut(s) 86, 185
SacI GAGCTC 1 cut(s) 428
SacII CCGCGG 1 cut(s) 290
SaqAI TTAA 3 cut(s) 266, 494, 569
SatI GCNGC 7 cut(s) 149, 194, 214, 287, 343, 358, 454
Sau3AI GATC 4 cut(s) 3, 9, 378, 476
Sau96I GGNCC 6 cut(s) 150, 384, 441, 472, 576, 633
SchI GAGTC 2 cut(s) 301, 316
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 428
SfaNI GCATC 1 cut(s) 251
SfcI CTRYAG 1 cut(s) 355
Sfr303I CCGCGG 1 cut(s) 290
SgrBI CCGCGG 1 cut(s) 290
SinI GGWCC 3 cut(s) 384, 441, 576
SmiMI CAYNNNNRTG 2 cut(s) 86, 185
SphI GCATGC 2 cut(s) 124, 220
Sse9I AATT 2 cut(s) 392, 566
SsiI CCGC 7 cut(s) 148, 246, 284, 287, 289, 329, 438
SstI GAGCTC 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 4 cut(s) 387, 480, 579, 636
TaaI ACNGT 1 cut(s) 264
TaiI ACGT 5 cut(s) 39, 45, 320, 450, 627
TaqI TCGA 1 cut(s) 6
TasI AATT 2 cut(s) 392, 566
TauI GCSGC 2 cut(s) 151, 289
Tru1I TTAA 3 cut(s) 266, 494, 569
Tru9I TTAA 3 cut(s) 266, 494, 569
TscAI CASTG 1 cut(s) 29
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 5 cut(s) 193, 213, 342, 357, 453
Tsp45I GTSAC 1 cut(s) 350
TspGWI ACGGA 3 cut(s) 44, 538, 606
TspRI CASTG 1 cut(s) 29
VpaK11BI GGWCC 3 cut(s) 384, 441, 576
XapI RAATTY 1 cut(s) 392
XceI RCATGY 2 cut(s) 124, 220
ZraI GACGTC 2 cut(s) 37, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.