Rroxscaffold_5G00340430

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013477
Physical Location & Seq
Forward (+)
8865810 .. 8869158
3349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00340430.1

Sequence Viewer

Length: 1854 bp
ATGTCGATCCTAAACCCATTCCAACTTCTGGAACTGAACGTGATCTCGGCACAAGACCTTGCCCCGGTATCACGTTCCATGCGGACGTACGCCATTGCATGGGTGCACCCGGATCGCAAGCTGTCGACCCGGGTGGACACGCACGGCCACAACAACCCTACGTGGAACGACAAGTTCGTGTTCCGGGTGGACGAGGATTTCTTGCATGAGGACACCTCCGCCGTGATGATCGAGATCTACGCCCTCCACTGGTTCAAAGACGTCCACGTAGGCACCATCCGTGTCCTCGTCGGCAATCTCATCCCGACTCCGGTGAAGCCCCACCACCACCATCCTCAGCTGGGCATGCGCTTCGTGGCGCTCCAGGTCCGGCGGCCCTCCGGCCGGCCACAGGGGATATTGAACATCGGTGTGGCGCTGCTTCACAGCTCCATGCGCAGCATGCCTTTGTATTCTCAGCTCAGCGCATCCGCCGTGGGATATAAACAGTTAATGGGGGAGGACGACCCGCCGCGGAGTCAAACCCACCGGAGTCAGTCAACGTTTTCGAAGGCGGAGCTGAGACGCAGCAAGAGTGACTGCAGCTCCATGTTAGCGTCGGAGGTCCGGACCAAGGAATTTACGAAAAAGGACAAAAAGAGCAGAGGGAGCTCGGTGGTCAGCGGGTCCGACGTCAGCAGCCAAAAGAATAAGAAGGGCCGATCCAAGGCCAGCTCGTTAATCAACGGCTCAGAAATCATCAGGGGCAGGGGTAGAAAAGGGAAAGCCAGCTCCGTGGTCAGCGCATCAGAATCAAGCCGGACCAAGGGCAAAAACGGAAAACCAAGTTCCATACTCAGCGTCTCCGACGTTGCTGGGGCCAAGGACAAAAAGGGCAAACCAAGTTCCATACTCAGCGTCTCCGAAATCAGCATCAGCGAGCCGTCAAAGAGAGCCGGTAGGGATCCGAAACCGACGAGCGGATCCGAACCCGACGACCCGGTGAGAAAGCCCAAGCACAACGGGAACTACAGAGCCGCGGTCGTACACGACATCGTGGCCGCGTCGCCGTTATCGAAGCCGTCACCGAAGTACGTGGTGGAGAGTTACGGAAATACCCCTGGGCGGAGGTCCTCGGTTCCGGGGAAGTCGCCGTTCAAGATGGGGACGTACGACCAGTACGTGACGCCGCGGAAGTCGAATCTGATGGTGCCGCCGTACCTGACGGAGTCGGAGCTGGGGCCTTCGCCGTCGGAGGTGGCTGCGGCAATCGCGAAGGAGAGGATGGACCAGGACAACGAGAGCTCGGTGGTGGTGGGGGCCTGGAACGAGGACGAGAGCGTGGAGGGGCTACAGTCAAAGCTGGAGCGGTGGCGGACGGAGCTGCCACCGGTGTACGATCGCGGCGATTTTTCAAGCTTTCCGAGCAGCAGCGAAGGGCACCACACGAGGCGGCACACAGACGGAGGCGGCGGCCCGGGGCTGTTCTCGTGCTTCAGTAACATCTGCGGCGTGGAGTGCTCCATCGTGTGCGGCGGCGGCGACGCCGCGGTGACTGGCTCCTTCACTAACTCCTCCTCTCACGACAATACGTTGTCGTTCGGGAGGTCCCTCGACAGTGGACCTGGTTCCGAGAAAGAGAGAGCCTCGCCATCGTCCGATGCGGTCGCTAACGATCCGGAATCCTTCGGTTTAGGTCGAGGACAGGTGGACGCAGAGATCCACGCCGCCGTTCTTTGGGGTGATGAAGCCGAACCCCTTCGGTCATTGAACCACTTCACCATTCGGTCAACCTCTCACTCAACCCTACGGCCGAGATTAGCGGATCCGTGGCGGATGTCGGCGTGCGGAGGACATGCAAGTGATGGTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

617

Amino Acids

66.8

Weight (kDa)

9.35

Isoelectric Point (pI)

56.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 9 - 107 6e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04540 AT2G33320
fragaria_vesca FvH4_4g04680
malus_domestica MD13G1222100.v1.1 MD16G1227000.v1.1
prunus_persica Prupe.1G049100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0395501
rosa_laevigata RLG00000009618
rosa_multiflora Rmu_sc0003334.1_g000002 Rmu_sc0020819.1_g000001
rosa_roxburghii Rroxscaffold_5G00340430
rosa_rugosa Rorug03G0355700
rosa_samantha Rh4AG060600 Rh4AG061800 Rh4BG059600 Rh4CG065300 Rh4DG055500
rosa_wichuraiana Rw4G005050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 264, 675
Acc16I TGCGCA 1 cut(s) 437
AccB1I GGYRCC 3 cut(s) 272, 1189, 1419
AccB7I CCANNNNNTGG 2 cut(s) 28, 99
AccBSI CCGCTC 2 cut(s) 960, 1348
AccI GTMKAC 1 cut(s) 125
AccII CGCG 7 cut(s) 514, 1019, 1043, 1171, 1253, 1383, 1529
AccIII TCCGGA 2 cut(s) 606, 1657
AclI AACGTT 1 cut(s) 542
AcoI YGGCCR 5 cut(s) 145, 382, 386, 1038, 1790
AcsI RAATTY 1 cut(s) 617
AcuI CTGAAG 1 cut(s) 1459
AcyI GRCGYC 4 cut(s) 261, 672, 1166, 1524
AfaI GTAC 7 cut(s) 89, 1026, 1073, 1151, 1160, 1199, 1376
AgeI ACCGGT 1 cut(s) 1369
AgsI TTSAA 5 cut(s) 256, 403, 1138, 1395, 1750
AjnI CCWGG 5 cut(s) 363, 1099, 1269, 1301, 1603
Alw21I GWGCWC 4 cut(s) 108, 653, 1286, 1502
Alw26I GTCTC 3 cut(s) 556, 847, 904
Alw44I GTGCAC 1 cut(s) 104
Ama87I CYCGRG 2 cut(s) 129, 1456
Aor13HI TCCGGA 2 cut(s) 606, 1657
ApaLI GTGCAC 1 cut(s) 104
ApeKI GCWGC 9 cut(s) 418, 438, 567, 582, 678, 1241, 1363, 1407, 1410
ApoI RAATTY 1 cut(s) 617
AsiGI ACCGGT 1 cut(s) 1369
Asp700I GAANNNNTTC 2 cut(s) 1737, 1754
AspLEI GCGC 6 cut(s) 351, 361, 418, 438, 467, 785
AsuC2I CCSGG 9 cut(s) 65, 110, 130, 131, 185, 980, 1122, 1457, 1458
AsuHPI GGTGA 6 cut(s) 325, 994, 1056, 1543, 1733, 1750
AsuII TTCGAA 1 cut(s) 548
AvaI CYCGRG 2 cut(s) 129, 1456
AvaII GGWCC 9 cut(s) 367, 604, 609, 666, 801, 1110, 1267, 1587, 1601
BaeGI GKGCMC 2 cut(s) 108, 1422
BamHI GGATCC 3 cut(s) 943, 962, 1804
BanI GGYRCC 3 cut(s) 272, 1189, 1419
BanII GRGCYC 2 cut(s) 653, 1286
BauI CACGAG 2 cut(s) 1426, 1468
Bbv12I GWGCWC 4 cut(s) 108, 653, 1286, 1502
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 9 cut(s) 405, 450, 579, 594, 690, 1228, 1350, 1419, 1422
BccI CCATC 8 cut(s) 284, 339, 1135, 1180, 1258, 1511, 1639, 1838
BcgI CGANNNNNNTGC 4 cut(s) 95, 129, 334, 368
BciT130I CCWGG 5 cut(s) 365, 1101, 1271, 1303, 1605
BcnI CCSGG 9 cut(s) 65, 110, 130, 131, 185, 980, 1122, 1457, 1458
BcoDI GTCTC 3 cut(s) 556, 847, 904
BfmI CTRYAG 3 cut(s) 580, 1009, 1331
BfoI RGCGCY 2 cut(s) 362, 419
BglII AGATCT 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 461
Bme18I GGWCC 9 cut(s) 367, 604, 609, 666, 801, 1110, 1267, 1587, 1601
BmeT110I CYCGRG 2 cut(s) 129, 1456
BmsI GCATC 4 cut(s) 476, 794, 921, 1630
BplI GAGNNNNNCTC 4 cut(s) 200, 232, 1610, 1642
BpmI CTGGAG 2 cut(s) 347, 1364
Bpu10I CCTNAGC 1 cut(s) 336
Bpu1102I GCTNAGC 1 cut(s) 461
Bpu14I TTCGAA 1 cut(s) 548
BpuMI CCSGG 9 cut(s) 65, 110, 130, 131, 185, 980, 1122, 1457, 1458
BsaAI YACGTR 4 cut(s) 162, 268, 1075, 1162
BsaBI GATNNNNATC 1 cut(s) 233
BsaHI GRCGYC 4 cut(s) 261, 672, 1166, 1524
BsaWI WCCGGW 5 cut(s) 310, 528, 606, 1369, 1657
BsaXI ACNNNNNCTCC 2 cut(s) 569, 599
Bse118I RCCGGY 3 cut(s) 384, 935, 1369
Bse1I ACTGG 3 cut(s) 254, 1156, 1540
Bse3DI GCAATG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 233
BseAI TCCGGA 2 cut(s) 606, 1657
BseBI CCWGG 5 cut(s) 365, 1101, 1271, 1303, 1605
BseGI GGATG 6 cut(s) 276, 300, 331, 467, 1269, 1821
BseJI GATNNNNATC 1 cut(s) 233
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 7 cut(s) 350, 470, 475, 551, 744, 850, 907
BseNI ACTGG 3 cut(s) 254, 1156, 1540
BseRI GAGGAG 2 cut(s) 1543, 1546
BseSI GKGCMC 2 cut(s) 108, 1422
BseX3I CGGCCG 2 cut(s) 382, 1790
BseXI GCAGC 9 cut(s) 405, 450, 579, 594, 690, 1228, 1350, 1419, 1422
BseYI CCCAGC 3 cut(s) 340, 854, 1216
Bsh1236I CGCG 7 cut(s) 514, 1019, 1043, 1171, 1253, 1383, 1529
Bsh1285I CGRYCG 5 cut(s) 385, 1023, 1381, 1647, 1793
BshNI GGYRCC 3 cut(s) 272, 1189, 1419
BshTI ACCGGT 1 cut(s) 1369
BsiEI CGRYCG 5 cut(s) 385, 1023, 1381, 1647, 1793
BsiHKAI GWGCWC 4 cut(s) 108, 653, 1286, 1502
BsiHKCI CYCGRG 2 cut(s) 129, 1456
BsiWI CGTACG 2 cut(s) 87, 1149
BslFI GGGAC 2 cut(s) 1159, 1573
BsmAI GTCTC 3 cut(s) 556, 847, 904
BsmBI CGTCTC 3 cut(s) 556, 847, 904
BsmFI GGGAC 2 cut(s) 1159, 1573
BsoBI CYCGRG 2 cut(s) 129, 1456
Bsp119I TTCGAA 1 cut(s) 548
Bsp1286I GDGCHC 5 cut(s) 108, 653, 1286, 1422, 1502
Bsp13I TCCGGA 2 cut(s) 606, 1657
Bsp1720I GCTNAGC 1 cut(s) 461
Bsp68I TCGCGA 1 cut(s) 1253
BspCNI CTCAG 7 cut(s) 349, 469, 474, 552, 743, 849, 906
BspEI TCCGGA 2 cut(s) 606, 1657
BspFNI CGCG 7 cut(s) 514, 1019, 1043, 1171, 1253, 1383, 1529
BspMAI CTGCAG 1 cut(s) 584
BspT104I TTCGAA 1 cut(s) 548
BspT107I GGYRCC 3 cut(s) 272, 1189, 1419
BsrBI CCGCTC 2 cut(s) 960, 1348
BsrDI GCAATG 1 cut(s) 93
BsrFI RCCGGY 3 cut(s) 384, 935, 1369
BsrI ACTGG 3 cut(s) 254, 1156, 1540
BssAI RCCGGY 3 cut(s) 384, 935, 1369
BssNI GRCGYC 4 cut(s) 261, 672, 1166, 1524
BssSI CACGAG 2 cut(s) 1426, 1468
BssT1I CCWWGG 4 cut(s) 612, 705, 804, 861
Bst2BI CACGAG 2 cut(s) 1426, 1468
Bst2UI CCWGG 5 cut(s) 365, 1101, 1271, 1303, 1605
Bst4CI ACNGT 3 cut(s) 489, 1335, 1598
BstACI GRCGYC 4 cut(s) 261, 672, 1166, 1524
BstBAI YACGTR 4 cut(s) 162, 268, 1075, 1162
BstBI TTCGAA 1 cut(s) 548
BstC8I GCNNGC 8 cut(s) 119, 347, 386, 443, 712, 769, 920, 1825
BstDEI CTNAG 7 cut(s) 336, 456, 461, 560, 730, 836, 893
BstDSI CCRYGG 7 cut(s) 474, 512, 774, 1017, 1169, 1527, 1808
BstF5I GGATG 6 cut(s) 276, 300, 331, 467, 1269, 1821
BstFNI CGCG 7 cut(s) 514, 1019, 1043, 1171, 1253, 1383, 1529
BstH2I RGCGCY 2 cut(s) 362, 419
BstHHI GCGC 6 cut(s) 351, 361, 418, 438, 467, 785
BstMAI GTCTC 3 cut(s) 556, 847, 904
BstMCI CGRYCG 5 cut(s) 385, 1023, 1381, 1647, 1793
BstMWI GCNNNNNNNGC 9 cut(s) 346, 435, 442, 648, 1250, 1360, 1404, 1497, 1518
BstNI CCWGG 5 cut(s) 365, 1101, 1271, 1303, 1605
BstNSI RCATGY 3 cut(s) 349, 445, 1838
BstSFI CTRYAG 3 cut(s) 580, 1009, 1331
BstSLI GKGCMC 2 cut(s) 108, 1422
BstUI CGCG 7 cut(s) 514, 1019, 1043, 1171, 1253, 1383, 1529
BstV1I GCAGC 9 cut(s) 405, 450, 579, 594, 690, 1228, 1350, 1419, 1422
BstX2I RGATCY 5 cut(s) 234, 943, 962, 1698, 1804
BstXI CCANNNNNNTGG 1 cut(s) 775
BstYI RGATCY 5 cut(s) 234, 943, 962, 1698, 1804
BstZI CGGCCG 2 cut(s) 382, 1790
BtgI CCRYGG 7 cut(s) 474, 512, 774, 1017, 1169, 1527, 1808
BtsCI GGATG 6 cut(s) 276, 300, 331, 467, 1269, 1821
BtsIMutI CAGTG 2 cut(s) 247, 1603
BtuMI TCGCGA 1 cut(s) 1253
Cac8I GCNNGC 8 cut(s) 119, 347, 386, 443, 712, 769, 920, 1825
CfoI GCGC 6 cut(s) 351, 361, 418, 438, 467, 785
Cfr10I RCCGGY 3 cut(s) 384, 935, 1369
Cfr42I CCGCGG 4 cut(s) 515, 1020, 1172, 1530
Cfr9I CCCGGG 2 cut(s) 129, 1456
CseI GACGC 8 cut(s) 573, 585, 829, 886, 1032, 1174, 1532, 1700
CsiI ACCWGGT 1 cut(s) 1603
Csp6I GTAC 7 cut(s) 88, 1025, 1072, 1150, 1159, 1198, 1375
CspAI ACCGGT 1 cut(s) 1369
CviAII CATG 8 cut(s) 79, 99, 206, 346, 433, 442, 589, 1835
CviQI GTAC 7 cut(s) 88, 1025, 1072, 1150, 1159, 1198, 1375
DdeI CTNAG 7 cut(s) 336, 456, 461, 560, 730, 836, 893
EaeI YGGCCR 5 cut(s) 145, 382, 386, 1038, 1790
EagI CGGCCG 2 cut(s) 382, 1790
EciI GGCGGA 6 cut(s) 208, 460, 569, 1120, 1369, 1828
Ecl136II GAGCTC 2 cut(s) 651, 1284
EclXI CGGCCG 2 cut(s) 382, 1790
Eco130I CCWWGG 4 cut(s) 612, 705, 804, 861
Eco24I GRGCYC 2 cut(s) 653, 1286
Eco47I GGWCC 9 cut(s) 367, 604, 609, 666, 801, 1110, 1267, 1587, 1601
Eco52I CGGCCG 2 cut(s) 382, 1790
Eco53kI GAGCTC 2 cut(s) 651, 1284
Eco57I CTGAAG 1 cut(s) 1459
Eco88I CYCGRG 2 cut(s) 129, 1456
EcoICRI GAGCTC 2 cut(s) 651, 1284
EcoO109I RGGNCCY 4 cut(s) 1110, 1220, 1299, 1587
EcoRII CCWGG 5 cut(s) 363, 1099, 1269, 1301, 1603
EcoT14I CCWWGG 4 cut(s) 612, 705, 804, 861
EcoT38I GRGCYC 2 cut(s) 653, 1286
ErhI CCWWGG 4 cut(s) 612, 705, 804, 861
Esp3I CGTCTC 3 cut(s) 556, 847, 904
FaeI CATG 8 cut(s) 82, 102, 209, 349, 436, 445, 592, 1838
FaqI GGGAC 2 cut(s) 1159, 1573
FatI CATG 8 cut(s) 78, 98, 205, 345, 432, 441, 588, 1834
FauI CCCGC 2 cut(s) 516, 656
FblI GTMKAC 1 cut(s) 125
FokI GGATG 6 cut(s) 263, 287, 318, 454, 1276, 1828
FriOI GRGCYC 2 cut(s) 653, 1286
FseI GGCCGGCC 1 cut(s) 388
FspI TGCGCA 1 cut(s) 437
GlaI GCGC 6 cut(s) 350, 360, 417, 437, 466, 784
GsaI CCCAGC 3 cut(s) 344, 858, 1220
GsuI CTGGAG 2 cut(s) 347, 1364
HaeII RGCGCY 2 cut(s) 362, 419
HgaI GACGC 8 cut(s) 573, 585, 829, 886, 1032, 1174, 1532, 1700
HhaI GCGC 6 cut(s) 351, 361, 418, 438, 467, 785
Hin1I GRCGYC 4 cut(s) 261, 672, 1166, 1524
Hin1II CATG 8 cut(s) 82, 102, 209, 349, 436, 445, 592, 1838
Hin6I GCGC 6 cut(s) 349, 359, 416, 436, 465, 783
HinP1I GCGC 6 cut(s) 349, 359, 416, 436, 465, 783
HincII GTYRAC 3 cut(s) 126, 540, 1770
HindII GTYRAC 3 cut(s) 126, 540, 1770
HindIII AAGCTT 1 cut(s) 1396
HinfI GANTC 7 cut(s) 307, 517, 532, 791, 1180, 1208, 1661
HphI GGTGA 6 cut(s) 325, 994, 1056, 1543, 1733, 1750
Hpy188III TCNNGA 9 cut(s) 29, 232, 304, 607, 1138, 1252, 1562, 1582, 1658
Hpy99I CGWCG 9 cut(s) 293, 601, 674, 851, 958, 977, 1048, 1234, 1526
HpyAV CCTTC 8 cut(s) 544, 688, 1233, 1249, 1409, 1552, 1675, 1748
HpyCH4III ACNGT 3 cut(s) 489, 1335, 1598
HpyCH4V TGCA 5 cut(s) 98, 106, 205, 582, 1838
HpyF10VI GCNNNNNNNGC 9 cut(s) 346, 435, 442, 648, 1250, 1360, 1404, 1497, 1518
HpyF3I CTNAG 7 cut(s) 336, 456, 461, 560, 730, 836, 893
Hsp92I GRCGYC 4 cut(s) 261, 672, 1166, 1524
Hsp92II CATG 8 cut(s) 82, 102, 209, 349, 436, 445, 592, 1838
HspAI GCGC 6 cut(s) 349, 359, 416, 436, 465, 783
Kpn2I TCCGGA 2 cut(s) 606, 1657
KroI GCCGGC 1 cut(s) 384
KroNI GCCGGC 1 cut(s) 386
KspI CCGCGG 4 cut(s) 515, 1020, 1172, 1530
Lsp1109I GCAGC 9 cut(s) 405, 450, 579, 594, 690, 1228, 1350, 1419, 1422
LweI GCATC 4 cut(s) 476, 794, 921, 1630
MabI ACCWGGT 1 cut(s) 1603
MaeIII GTNAC 6 cut(s) 575, 1062, 1085, 1162, 1478, 1531
MbiI CCGCTC 2 cut(s) 960, 1348
MflI RGATCY 5 cut(s) 234, 943, 962, 1698, 1804
MhlI GDGCHC 5 cut(s) 108, 653, 1286, 1422, 1502
MluCI AATT 1 cut(s) 617
MlyI GAGTC 4 cut(s) 301, 526, 541, 1217
MmeI TCCRAC 6 cut(s) 46, 579, 693, 870, 1191, 1212
MroI TCCGGA 2 cut(s) 606, 1657
MroNI GCCGGC 1 cut(s) 384
MroXI GAANNNNTTC 2 cut(s) 1737, 1754
MseI TTAA 3 cut(s) 491, 719, 1852
MslI CAYNNNNRTG 2 cut(s) 410, 1839
MspA1I CMGCKG 6 cut(s) 340, 514, 663, 1019, 1171, 1529
MvaI CCWGG 5 cut(s) 365, 1101, 1271, 1303, 1605
MvnI CGCG 7 cut(s) 514, 1019, 1043, 1171, 1253, 1383, 1529
MwoI GCNNNNNNNGC 9 cut(s) 346, 435, 442, 648, 1250, 1360, 1404, 1497, 1518
NaeI GCCGGC 1 cut(s) 386
NciI CCSGG 9 cut(s) 65, 110, 130, 131, 185, 980, 1122, 1457, 1458
NgoMIV GCCGGC 1 cut(s) 384
NlaIII CATG 8 cut(s) 82, 102, 209, 349, 436, 445, 592, 1838
NmeAIII GCCGAG 2 cut(s) 26, 1818
NmuCI GTSAC 4 cut(s) 575, 1062, 1162, 1531
NruI TCGCGA 1 cut(s) 1253
NsbI TGCGCA 1 cut(s) 437
NspI RCATGY 3 cut(s) 349, 445, 1838
NspV TTCGAA 1 cut(s) 548
PaeI GCATGC 2 cut(s) 349, 445
PasI CCCWGGG 1 cut(s) 1100
PcsI WCGNNNNNNNCGW 4 cut(s) 174, 1052, 1158, 1383
PdiI GCCGGC 1 cut(s) 386
PdmI GAANNNNTTC 2 cut(s) 1737, 1754
PfeI GAWTC 3 cut(s) 791, 1180, 1661
Pfl23II CGTACG 2 cut(s) 87, 1149
PflFI GACNNNGTC 1 cut(s) 1207
PflMI CCANNNNNTGG 2 cut(s) 28, 99
PinAI ACCGGT 1 cut(s) 1369
Ple19I CGATCG 1 cut(s) 1381
PleI GAGTC 4 cut(s) 301, 525, 540, 1216
PpsI GAGTC 4 cut(s) 301, 525, 540, 1216
Ppu21I YACGTR 4 cut(s) 162, 268, 1075, 1162
PpuMI RGGWCCY 2 cut(s) 1110, 1587
Psp124BI GAGCTC 2 cut(s) 653, 1286
Psp1406I AACGTT 1 cut(s) 542
Psp5II RGGWCCY 2 cut(s) 1110, 1587
Psp6I CCWGG 5 cut(s) 363, 1099, 1269, 1301, 1603
PspFI CCCAGC 3 cut(s) 340, 854, 1216
PspGI CCWGG 5 cut(s) 363, 1099, 1269, 1301, 1603
PspLI CGTACG 2 cut(s) 87, 1149
PspPPI RGGWCCY 2 cut(s) 1110, 1587
PstI CTGCAG 1 cut(s) 584
PsuI RGATCY 5 cut(s) 234, 943, 962, 1698, 1804
PsyI GACNNNGTC 1 cut(s) 1207
PvuI CGATCG 1 cut(s) 1381
PvuII CAGCTG 1 cut(s) 340
RigI GGCCGGCC 1 cut(s) 388
RruI TCGCGA 1 cut(s) 1253
RsaI GTAC 7 cut(s) 89, 1026, 1073, 1151, 1160, 1199, 1376
RsaNI GTAC 7 cut(s) 88, 1025, 1072, 1150, 1159, 1198, 1375
RseI CAYNNNNRTG 2 cut(s) 410, 1839
SacI GAGCTC 2 cut(s) 653, 1286
SacII CCGCGG 4 cut(s) 515, 1020, 1172, 1530
SalI GTCGAC 1 cut(s) 124
SaqAI TTAA 3 cut(s) 491, 719, 1852
SchI GAGTC 4 cut(s) 301, 526, 541, 1217
SduI GDGCHC 5 cut(s) 108, 653, 1286, 1422, 1502
SexAI ACCWGGT 1 cut(s) 1603
SfaNI GCATC 4 cut(s) 476, 794, 921, 1630
SfcI CTRYAG 3 cut(s) 580, 1009, 1331
Sfr303I CCGCGG 4 cut(s) 515, 1020, 1172, 1530
SfuI TTCGAA 1 cut(s) 548
SgrAI CRCCGGYG 1 cut(s) 1369
SgrBI CCGCGG 4 cut(s) 515, 1020, 1172, 1530
SinI GGWCC 9 cut(s) 367, 604, 609, 666, 801, 1110, 1267, 1587, 1601
SmaI CCCGGG 2 cut(s) 131, 1458
SmiMI CAYNNNNRTG 2 cut(s) 410, 1839
SphI GCATGC 2 cut(s) 349, 445
Sse9I AATT 1 cut(s) 617
SstI GAGCTC 2 cut(s) 653, 1286
StyI CCWWGG 4 cut(s) 612, 705, 804, 861
TaaI ACNGT 3 cut(s) 489, 1335, 1598
TaqI TCGA 8 cut(s) 5, 125, 231, 548, 1055, 1178, 1593, 1678
TaqII GACCGA 2 cut(s) 1731, 1755
TasI AATT 1 cut(s) 617
TfiI GAWTC 3 cut(s) 791, 1180, 1661
Tru1I TTAA 3 cut(s) 491, 719, 1852
Tru9I TTAA 3 cut(s) 491, 719, 1852
TscAI CASTG 2 cut(s) 254, 1603
TseFI GTSAC 4 cut(s) 575, 1062, 1162, 1531
TseI GCWGC 9 cut(s) 418, 438, 567, 582, 678, 1241, 1363, 1407, 1410
Tsp45I GTSAC 4 cut(s) 575, 1062, 1162, 1531
TspDTI ATGAA 1 cut(s) 1740
TspGWI ACGGA 8 cut(s) 269, 763, 831, 1104, 1220, 1373, 1458, 1797
TspMI CCCGGG 2 cut(s) 129, 1456
TspRI CASTG 2 cut(s) 254, 1603
Tth111I GACNNNGTC 1 cut(s) 1207
Van91I CCANNNNNTGG 2 cut(s) 28, 99
VneI GTGCAC 1 cut(s) 104
VpaK11BI GGWCC 9 cut(s) 367, 604, 609, 666, 801, 1110, 1267, 1587, 1601
XapI RAATTY 1 cut(s) 617
XceI RCATGY 3 cut(s) 349, 445, 1838
XmaI CCCGGG 2 cut(s) 129, 1456
XmiI GTMKAC 1 cut(s) 125
XmnI GAANNNNTTC 2 cut(s) 1737, 1754
ZraI GACGTC 2 cut(s) 262, 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.