RLG00000009618

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013477
Physical Location & Seq
Reverse (-)
55456538 .. 55458151
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009618

Sequence Viewer

Length: 1614 bp
ATGTCGATCCTAAACCCATTCCAACTTCTGGAACTGAACGTGATCTCGGCGCAAGACCTTGCCCCGGTATCACGTTCCATGCGGACGTACGCCATTGCATGGGTGCACCCGGATCGCAAGCTGTCCACCCGGGTGGACACGCACGGCCACAACAACCCTACGTGGAACGACAAGTTCGTGTTCCGGGTGGACGAGGATTTCTTGCATGAGGACACCTCCGCCGTGATGATCGAGATCTACGCCCTCCACTGGTTCAAAGACGTCCACGTCGGTACCATCCGTGTCCTCGTCGGCAATCTCATCCCGACTCCGGTGAAGCCCCACCACCACCATCCTCAGCTGGGCATGCGCTTCGTGGCGCTCCAGGTCCGGCGGCCCTCCGGCCGGCCACAGGGGATATTGAACATCGGTGTGGCGCTGCTTCACAGCTCCATGCGCAGCATGCCTTTGTATTCTCAGCTCAGCGCATCCGCCGTGGGATATAAACAGTTAATGGGGGAGGACGACCCGCCGCGGAGTCAAACCCACCGGAGTCAGTCAACGTTTTCGAAGGCGGAGCTGAGACGTAGCAAGAGTGACTGCAGCTCCATGTTAGCGTCGGAGGTCCGGACCAAGGAATTTACGAAAAAGGACAAAAAGAGCGGAGGGAGCTCGGTGGTCAGCGGGTCCGACGTTAGCGGCCAAAAGACTAAGAAGGGCCGATCCAAGGCCAGCTCGTTAATCAACGGCTCAGAAATCATCAGGGGCAGGGGTAGAAAAGGGAAAGCCAGCTCCGTGGTCAGCGCATCAGAATTAAGTCGAACCGAGGGTAAAAACGGAAAACCAAGTTCCATACTCAGCGTCTCCGACGTTGGCGGTGGCAAGGACAAAAAGGGAAAACCAAGTTCCATACTCAGCGTCTCCGAAATAAGCGTCAGCGACCCGTCAAAGAGAGGCGGTAGGGATCCGAAACCGACGAGCGGATCCGAACCCGACGACCCAGTAAGAAAGCCCAAGCACAACGGGAACTACAGAGCCGCAGTCGTACACGACATCGTGGCGGCGTCGCCGTTATCGAAGCAGTCACCGAAGTACATGATGGAGAGTTACGGAAATACCCCTGGGCGGAGGTCCTCGGTTCCGGGGAAGTCGCCGTTCAAGATGGGGACGTACGACCAGTACGTGACGCCGCGGAAGTCGAATCTGATGGTGCCGCCGTACTTGACGGAGTCGGAGCTGGGGCCGTCGCCGTCCGAGGTGGCGGCGGCGATCGCGAAGGAGAGGATGGACCAGGACAACGAGAGCTCGGTGGTGGTGGGGGCTTGGAACGAGGACGAGAGCGTGGAGGGGCTACAGTCGAAACTGGAGCGGTGGCGGACGGAGCTGCCGCCGGTGTACGATCGCGGAGATTTTTCGAGCTTTCCGAGCAGCAGCGAAGGGCACCACACGCGGCGGCACACAGAAGGAGGTGGCGGCCCGGGGTTGTTCTCGTGCTTCAGTAACATCTGCGGTGTGGAGTGCTCCATCGTGTGCGGCGGCGGCGACGCCGGTAAGGATCGGAAGAAGGCGGGGGGCAGCAAGAGTAGCGGTCGGGTCGGGCGGAGCCCATCAGCGGAGAATTTGAGCTATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

538

Amino Acids

58.2

Weight (kDa)

9.61

Isoelectric Point (pI)

57.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 9 - 107 4.8e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04540 AT2G33320
fragaria_vesca FvH4_4g04680
malus_domestica MD13G1222100.v1.1 MD16G1227000.v1.1
prunus_persica Prupe.1G049100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0395501
rosa_laevigata RLG00000009618
rosa_multiflora Rmu_sc0003334.1_g000002 Rmu_sc0020819.1_g000001
rosa_roxburghii Rroxscaffold_5G00340430
rosa_rugosa Rorug03G0355700
rosa_samantha Rh4AG060600 Rh4AG061800 Rh4BG059600 Rh4CG065300 Rh4DG055500
rosa_wichuraiana Rw4G005050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
AatII GACGTC 1 cut(s) 264
Acc16I TGCGCA 1 cut(s) 437
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 3 cut(s) 272, 1189, 1419
AccB7I CCANNNNNTGG 2 cut(s) 28, 99
AccBSI CCGCTC 3 cut(s) 642, 960, 1348
AccII CGCG 5 cut(s) 514, 1171, 1253, 1383, 1429
AccIII TCCGGA 1 cut(s) 606
AclI AACGTT 1 cut(s) 542
AclWI GGATC 7 cut(s) 120, 696, 938, 951, 957, 970, 1542
AcoI YGGCCR 4 cut(s) 145, 382, 386, 679
AcsI RAATTY 2 cut(s) 617, 1597
AcuI CTGAAG 1 cut(s) 1459
AcyI GRCGYC 4 cut(s) 261, 1043, 1166, 1524
AfaI GTAC 8 cut(s) 89, 274, 1026, 1073, 1151, 1160, 1199, 1376
AgsI TTSAA 3 cut(s) 256, 403, 1138
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 3 cut(s) 363, 1099, 1269
AleI CACNNNNGTG 1 cut(s) 131
Alw21I GWGCWC 4 cut(s) 108, 653, 1286, 1502
Alw26I GTCTC 3 cut(s) 556, 847, 904
Alw44I GTGCAC 1 cut(s) 104
AlwI GGATC 7 cut(s) 120, 696, 938, 951, 957, 970, 1542
Ama87I CYCGRG 2 cut(s) 129, 1456
Aor13HI TCCGGA 1 cut(s) 606
AoxI GGCC 9 cut(s) 145, 374, 382, 386, 679, 697, 708, 1220, 1453
ApaLI GTGCAC 1 cut(s) 104
ApeKI GCWGC 7 cut(s) 418, 438, 582, 1363, 1407, 1410, 1554
ApoI RAATTY 2 cut(s) 617, 1597
ArsI GACNNNNNNTTYG 2 cut(s) 897, 929
AsiSI GCGATCGC 1 cut(s) 1251
Asp718I GGTACC 1 cut(s) 272
AspLEI GCGC 7 cut(s) 52, 351, 361, 418, 438, 467, 785
AsuC2I CCSGG 8 cut(s) 65, 110, 130, 131, 185, 1122, 1457, 1458
AsuHPI GGTGA 2 cut(s) 325, 1056
AsuII TTCGAA 1 cut(s) 548
AvaI CYCGRG 2 cut(s) 129, 1456
AvaII GGWCC 6 cut(s) 367, 604, 609, 666, 1110, 1267
BaeGI GKGCMC 2 cut(s) 108, 1422
BaeI ACNNNNGTAYC 2 cut(s) 256, 289
BamHI GGATCC 2 cut(s) 943, 962
BanI GGYRCC 3 cut(s) 272, 1189, 1419
BanII GRGCYC 3 cut(s) 653, 1286, 1586
BauI CACGAG 1 cut(s) 1468
Bbv12I GWGCWC 4 cut(s) 108, 653, 1286, 1502
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 7 cut(s) 405, 450, 594, 1350, 1419, 1422, 1566
BccI CCATC 8 cut(s) 284, 339, 1072, 1135, 1180, 1258, 1511, 1594
BceAI ACGGC 9 cut(s) 160, 206, 458, 742, 1033, 1117, 1180, 1207, 1213
BcgI CGANNNNNNTGC 4 cut(s) 95, 129, 334, 368
BciT130I CCWGG 3 cut(s) 365, 1101, 1271
BcnI CCSGG 8 cut(s) 65, 110, 130, 131, 185, 1122, 1457, 1458
BcoDI GTCTC 3 cut(s) 556, 847, 904
BfmI CTRYAG 3 cut(s) 580, 1009, 1331
BfoI RGCGCY 2 cut(s) 362, 419
BglII AGATCT 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 461
Bme18I GGWCC 6 cut(s) 367, 604, 609, 666, 1110, 1267
BmeT110I CYCGRG 2 cut(s) 129, 1456
BmgBI CACGTC 1 cut(s) 268
BmiI GGNNCC 9 cut(s) 274, 667, 945, 964, 1119, 1191, 1221, 1421, 1583
BmrI ACTGGG 1 cut(s) 974
BmsI GCATC 2 cut(s) 476, 794
BmuI ACTGGG 1 cut(s) 974
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 2 cut(s) 347, 1364
Bpu10I CCTNAGC 1 cut(s) 336
Bpu1102I GCTNAGC 1 cut(s) 461
Bpu14I TTCGAA 1 cut(s) 548
BpuMI CCSGG 8 cut(s) 65, 110, 130, 131, 185, 1122, 1457, 1458
BsaAI YACGTR 2 cut(s) 162, 1162
BsaBI GATNNNNATC 1 cut(s) 233
BsaHI GRCGYC 4 cut(s) 261, 1043, 1166, 1524
BsaWI WCCGGW 3 cut(s) 310, 528, 606
BsaXI ACNNNNNCTCC 2 cut(s) 569, 599
Bse118I RCCGGY 3 cut(s) 384, 1369, 1526
Bse1I ACTGG 4 cut(s) 254, 980, 1156, 1347
Bse3DI GCAATG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 233
BseAI TCCGGA 1 cut(s) 606
BseBI CCWGG 3 cut(s) 365, 1101, 1271
BseGI GGATG 5 cut(s) 276, 300, 331, 467, 1269
BseJI GATNNNNATC 1 cut(s) 233
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 7 cut(s) 350, 470, 475, 551, 744, 850, 907
BseNI ACTGG 4 cut(s) 254, 980, 1156, 1347
BseSI GKGCMC 2 cut(s) 108, 1422
BseX3I CGGCCG 1 cut(s) 382
BseXI GCAGC 7 cut(s) 405, 450, 594, 1350, 1419, 1422, 1566
BseYI CCCAGC 2 cut(s) 340, 1216
Bsh1236I CGCG 5 cut(s) 514, 1171, 1253, 1383, 1429
Bsh1285I CGRYCG 4 cut(s) 385, 1251, 1381, 1570
BshFI GGCC 9 cut(s) 147, 376, 384, 388, 681, 699, 710, 1222, 1455
BshNI GGYRCC 3 cut(s) 272, 1189, 1419
BsiEI CGRYCG 4 cut(s) 385, 1251, 1381, 1570
BsiHKAI GWGCWC 4 cut(s) 108, 653, 1286, 1502
BsiHKCI CYCGRG 2 cut(s) 129, 1456
BsiWI CGTACG 2 cut(s) 87, 1149
BslFI GGGAC 1 cut(s) 1159
BsmAI GTCTC 3 cut(s) 556, 847, 904
BsmBI CGTCTC 3 cut(s) 556, 847, 904
BsmFI GGGAC 1 cut(s) 1159
BsnI GGCC 9 cut(s) 147, 376, 384, 388, 681, 699, 710, 1222, 1455
BsoBI CYCGRG 2 cut(s) 129, 1456
Bsp119I TTCGAA 1 cut(s) 548
Bsp1286I GDGCHC 6 cut(s) 108, 653, 1286, 1422, 1502, 1586
Bsp13I TCCGGA 1 cut(s) 606
Bsp1720I GCTNAGC 1 cut(s) 461
Bsp68I TCGCGA 1 cut(s) 1253
BspANI GGCC 9 cut(s) 147, 376, 384, 388, 681, 699, 710, 1222, 1455
BspCNI CTCAG 7 cut(s) 349, 469, 474, 552, 743, 849, 906
BspEI TCCGGA 1 cut(s) 606
BspFNI CGCG 5 cut(s) 514, 1171, 1253, 1383, 1429
BspLI GGNNCC 9 cut(s) 274, 667, 945, 964, 1119, 1191, 1221, 1421, 1583
BspMAI CTGCAG 1 cut(s) 584
BspPI GGATC 7 cut(s) 120, 696, 938, 951, 957, 970, 1542
BspT104I TTCGAA 1 cut(s) 548
BspT107I GGYRCC 3 cut(s) 272, 1189, 1419
BsrBI CCGCTC 3 cut(s) 642, 960, 1348
BsrDI GCAATG 1 cut(s) 93
BsrFI RCCGGY 3 cut(s) 384, 1369, 1526
BsrI ACTGG 4 cut(s) 254, 980, 1156, 1347
BssAI RCCGGY 3 cut(s) 384, 1369, 1526
BssNI GRCGYC 4 cut(s) 261, 1043, 1166, 1524
BssSI CACGAG 1 cut(s) 1468
BssT1I CCWWGG 2 cut(s) 612, 705
Bst2BI CACGAG 1 cut(s) 1468
Bst2UI CCWGG 3 cut(s) 365, 1101, 1271
Bst4CI ACNGT 2 cut(s) 489, 1335
BstACI GRCGYC 4 cut(s) 261, 1043, 1166, 1524
BstBAI YACGTR 2 cut(s) 162, 1162
BstBI TTCGAA 1 cut(s) 548
BstC8I GCNNGC 6 cut(s) 119, 347, 386, 443, 712, 769
BstDEI CTNAG 8 cut(s) 336, 456, 461, 560, 690, 730, 836, 893
BstDSI CCRYGG 4 cut(s) 474, 512, 774, 1169
BstF5I GGATG 5 cut(s) 276, 300, 331, 467, 1269
BstFNI CGCG 5 cut(s) 514, 1171, 1253, 1383, 1429
BstH2I RGCGCY 2 cut(s) 362, 419
BstHHI GCGC 7 cut(s) 52, 351, 361, 418, 438, 467, 785
BstMAI GTCTC 3 cut(s) 556, 847, 904
BstMCI CGRYCG 4 cut(s) 385, 1251, 1381, 1570
BstNI CCWGG 3 cut(s) 365, 1101, 1271
BstNSI RCATGY 2 cut(s) 349, 445
BstSFI CTRYAG 3 cut(s) 580, 1009, 1331
BstSLI GKGCMC 2 cut(s) 108, 1422
BstUI CGCG 5 cut(s) 514, 1171, 1253, 1383, 1429
BstV1I GCAGC 7 cut(s) 405, 450, 594, 1350, 1419, 1422, 1566
BstX2I RGATCY 3 cut(s) 234, 943, 962
BstXI CCANNNNNNTGG 2 cut(s) 133, 775
BstYI RGATCY 3 cut(s) 234, 943, 962
BstZI CGGCCG 1 cut(s) 382
BsuRI GGCC 9 cut(s) 147, 376, 384, 388, 681, 699, 710, 1222, 1455
BtgI CCRYGG 4 cut(s) 474, 512, 774, 1169
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 5 cut(s) 276, 300, 331, 467, 1269
BtsIMutI CAGTG 1 cut(s) 247
BtuMI TCGCGA 1 cut(s) 1253
Cac8I GCNNGC 6 cut(s) 119, 347, 386, 443, 712, 769
CfoI GCGC 7 cut(s) 52, 351, 361, 418, 438, 467, 785
Cfr10I RCCGGY 3 cut(s) 384, 1369, 1526
Cfr42I CCGCGG 2 cut(s) 515, 1172
Cfr9I CCCGGG 2 cut(s) 129, 1456
CseI GACGC 7 cut(s) 585, 829, 886, 901, 1032, 1174, 1532
Csp6I GTAC 8 cut(s) 88, 273, 1025, 1072, 1150, 1159, 1198, 1375
CviAII CATG 8 cut(s) 79, 99, 206, 346, 433, 442, 589, 1075
CviQI GTAC 8 cut(s) 88, 273, 1025, 1072, 1150, 1159, 1198, 1375
DdeI CTNAG 8 cut(s) 336, 456, 461, 560, 690, 730, 836, 893
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 4 cut(s) 145, 382, 386, 679
EagI CGGCCG 1 cut(s) 382
EciI GGCGGA 6 cut(s) 208, 460, 569, 1120, 1369, 1594
Ecl136II GAGCTC 2 cut(s) 651, 1284
EclXI CGGCCG 1 cut(s) 382
Eco130I CCWWGG 2 cut(s) 612, 705
Eco24I GRGCYC 3 cut(s) 653, 1286, 1586
Eco47I GGWCC 6 cut(s) 367, 604, 609, 666, 1110, 1267
Eco52I CGGCCG 1 cut(s) 382
Eco53kI GAGCTC 2 cut(s) 651, 1284
Eco57I CTGAAG 1 cut(s) 1459
Eco88I CYCGRG 2 cut(s) 129, 1456
EcoICRI GAGCTC 2 cut(s) 651, 1284
EcoO109I RGGNCCY 1 cut(s) 1110
EcoRII CCWGG 3 cut(s) 363, 1099, 1269
EcoT14I CCWWGG 2 cut(s) 612, 705
EcoT38I GRGCYC 3 cut(s) 653, 1286, 1586
ErhI CCWWGG 2 cut(s) 612, 705
Esp3I CGTCTC 3 cut(s) 556, 847, 904
FaeI CATG 8 cut(s) 82, 102, 209, 349, 436, 445, 592, 1078
FaqI GGGAC 1 cut(s) 1159
FatI CATG 8 cut(s) 78, 98, 205, 345, 432, 441, 588, 1074
FauI CCCGC 3 cut(s) 516, 656, 1540
FokI GGATG 5 cut(s) 263, 287, 318, 454, 1276
FriOI GRGCYC 3 cut(s) 653, 1286, 1586
FseI GGCCGGCC 1 cut(s) 388
FspI TGCGCA 1 cut(s) 437
GlaI GCGC 7 cut(s) 51, 350, 360, 417, 437, 466, 784
GsaI CCCAGC 2 cut(s) 344, 1220
GsuI CTGGAG 2 cut(s) 347, 1364
HaeII RGCGCY 2 cut(s) 362, 419
HaeIII GGCC 9 cut(s) 147, 376, 384, 388, 681, 699, 710, 1222, 1455
HgaI GACGC 7 cut(s) 585, 829, 886, 901, 1032, 1174, 1532
HhaI GCGC 7 cut(s) 52, 351, 361, 418, 438, 467, 785
Hin1I GRCGYC 4 cut(s) 261, 1043, 1166, 1524
Hin1II CATG 8 cut(s) 82, 102, 209, 349, 436, 445, 592, 1078
Hin6I GCGC 7 cut(s) 50, 349, 359, 416, 436, 465, 783
HinP1I GCGC 7 cut(s) 50, 349, 359, 416, 436, 465, 783
HincII GTYRAC 1 cut(s) 540
HindII GTYRAC 1 cut(s) 540
HinfI GANTC 5 cut(s) 307, 517, 532, 1180, 1208
HphI GGTGA 2 cut(s) 325, 1056
Hpy166II GTNNAC 8 cut(s) 106, 126, 136, 190, 265, 540, 1027, 1375
Hpy188III TCNNGA 6 cut(s) 29, 232, 304, 607, 1138, 1252
Hpy8I GTNNAC 8 cut(s) 106, 126, 136, 190, 265, 540, 1027, 1375
HpyAV CCTTC 6 cut(s) 544, 688, 1249, 1409, 1436, 1537
HpyCH4III ACNGT 2 cut(s) 489, 1335
HpyCH4V TGCA 4 cut(s) 98, 106, 205, 582
HpyF3I CTNAG 8 cut(s) 336, 456, 461, 560, 690, 730, 836, 893
Hsp92I GRCGYC 4 cut(s) 261, 1043, 1166, 1524
Hsp92II CATG 8 cut(s) 82, 102, 209, 349, 436, 445, 592, 1078
HspAI GCGC 7 cut(s) 50, 349, 359, 416, 436, 465, 783
Kpn2I TCCGGA 1 cut(s) 606
KpnI GGTACC 1 cut(s) 276
KroI GCCGGC 1 cut(s) 384
KroNI GCCGGC 1 cut(s) 386
KspI CCGCGG 2 cut(s) 515, 1172
Lsp1109I GCAGC 7 cut(s) 405, 450, 594, 1350, 1419, 1422, 1566
LweI GCATC 2 cut(s) 476, 794
MaeIII GTNAC 5 cut(s) 575, 1062, 1085, 1162, 1478
MbiI CCGCTC 3 cut(s) 642, 960, 1348
MboII GAAGA 1 cut(s) 1552
MflI RGATCY 3 cut(s) 234, 943, 962
MhlI GDGCHC 6 cut(s) 108, 653, 1286, 1422, 1502, 1586
MluCI AATT 3 cut(s) 617, 791, 1597
MlyI GAGTC 4 cut(s) 301, 526, 541, 1217
MmeI TCCRAC 5 cut(s) 46, 579, 693, 870, 1191
MroI TCCGGA 1 cut(s) 606
MroNI GCCGGC 1 cut(s) 384
MseI TTAA 3 cut(s) 491, 719, 794
MslI CAYNNNNRTG 2 cut(s) 131, 410
MspA1I CMGCKG 5 cut(s) 340, 514, 663, 1171, 1592
MvaI CCWGG 3 cut(s) 365, 1101, 1271
MvnI CGCG 5 cut(s) 514, 1171, 1253, 1383, 1429
NaeI GCCGGC 1 cut(s) 386
NciI CCSGG 8 cut(s) 65, 110, 130, 131, 185, 1122, 1457, 1458
NgoMIV GCCGGC 1 cut(s) 384
NlaIII CATG 8 cut(s) 82, 102, 209, 349, 436, 445, 592, 1078
NlaIV GGNNCC 9 cut(s) 274, 667, 945, 964, 1119, 1191, 1221, 1421, 1583
NmeAIII GCCGAG 1 cut(s) 26
NmuCI GTSAC 3 cut(s) 575, 1062, 1162
NruI TCGCGA 1 cut(s) 1253
NsbI TGCGCA 1 cut(s) 437
NspI RCATGY 2 cut(s) 349, 445
NspV TTCGAA 1 cut(s) 548
OliI CACNNNNGTG 1 cut(s) 131
PaeI GCATGC 2 cut(s) 349, 445
PasI CCCWGGG 1 cut(s) 1100
PcsI WCGNNNNNNNCGW 4 cut(s) 174, 1052, 1158, 1400
PdiI GCCGGC 1 cut(s) 386
PfeI GAWTC 1 cut(s) 1180
Pfl23II CGTACG 2 cut(s) 87, 1149
PflFI GACNNNGTC 1 cut(s) 1207
PflMI CCANNNNNTGG 2 cut(s) 28, 99
Ple19I CGATCG 2 cut(s) 1251, 1381
PleI GAGTC 4 cut(s) 301, 525, 540, 1216
PpsI GAGTC 4 cut(s) 301, 525, 540, 1216
Ppu21I YACGTR 2 cut(s) 162, 1162
PpuMI RGGWCCY 1 cut(s) 1110
Psp124BI GAGCTC 2 cut(s) 653, 1286
Psp1406I AACGTT 1 cut(s) 542
Psp5II RGGWCCY 1 cut(s) 1110
Psp6I CCWGG 3 cut(s) 363, 1099, 1269
PspFI CCCAGC 2 cut(s) 340, 1216
PspGI CCWGG 3 cut(s) 363, 1099, 1269
PspLI CGTACG 2 cut(s) 87, 1149
PspN4I GGNNCC 9 cut(s) 274, 667, 945, 964, 1119, 1191, 1221, 1421, 1583
PspPPI RGGWCCY 1 cut(s) 1110
PstI CTGCAG 1 cut(s) 584
PsuI RGATCY 3 cut(s) 234, 943, 962
PsyI GACNNNGTC 1 cut(s) 1207
PvuI CGATCG 2 cut(s) 1251, 1381
PvuII CAGCTG 1 cut(s) 340
RgaI GCGATCGC 1 cut(s) 1251
RigI GGCCGGCC 1 cut(s) 388
RruI TCGCGA 1 cut(s) 1253
RsaI GTAC 8 cut(s) 89, 274, 1026, 1073, 1151, 1160, 1199, 1376
RsaNI GTAC 8 cut(s) 88, 273, 1025, 1072, 1150, 1159, 1198, 1375
RseI CAYNNNNRTG 2 cut(s) 131, 410
SacI GAGCTC 2 cut(s) 653, 1286
SacII CCGCGG 2 cut(s) 515, 1172
SaqAI TTAA 3 cut(s) 491, 719, 794
SchI GAGTC 4 cut(s) 301, 526, 541, 1217
SduI GDGCHC 6 cut(s) 108, 653, 1286, 1422, 1502, 1586
SfaAI GCGATCGC 1 cut(s) 1251
SfaNI GCATC 2 cut(s) 476, 794
SfcI CTRYAG 3 cut(s) 580, 1009, 1331
Sfr303I CCGCGG 2 cut(s) 515, 1172
SfuI TTCGAA 1 cut(s) 548
SgfI GCGATCGC 1 cut(s) 1251
SgrAI CRCCGGYG 1 cut(s) 1369
SgrBI CCGCGG 2 cut(s) 515, 1172
SinI GGWCC 6 cut(s) 367, 604, 609, 666, 1110, 1267
SmaI CCCGGG 2 cut(s) 131, 1458
SmiMI CAYNNNNRTG 2 cut(s) 131, 410
SphI GCATGC 2 cut(s) 349, 445
Sse9I AATT 3 cut(s) 617, 791, 1597
SstI GAGCTC 2 cut(s) 653, 1286
StyI CCWWGG 2 cut(s) 612, 705
TaaI ACNGT 2 cut(s) 489, 1335
TaqI TCGA 8 cut(s) 5, 231, 548, 799, 1055, 1178, 1337, 1394
TasI AATT 3 cut(s) 617, 791, 1597
TatI WGTACW 1 cut(s) 1071
TfiI GAWTC 1 cut(s) 1180
Tru1I TTAA 3 cut(s) 491, 719, 794
Tru9I TTAA 3 cut(s) 491, 719, 794
TscAI CASTG 1 cut(s) 254
TseFI GTSAC 3 cut(s) 575, 1062, 1162
TseI GCWGC 7 cut(s) 418, 438, 582, 1363, 1407, 1410, 1554
Tsp45I GTSAC 3 cut(s) 575, 1062, 1162
TspGWI ACGGA 6 cut(s) 269, 763, 831, 1104, 1220, 1373
TspMI CCCGGG 2 cut(s) 129, 1456
TspRI CASTG 1 cut(s) 254
Tth111I GACNNNGTC 1 cut(s) 1207
Van91I CCANNNNNTGG 2 cut(s) 28, 99
VneI GTGCAC 1 cut(s) 104
VpaK11BI GGWCC 6 cut(s) 367, 604, 609, 666, 1110, 1267
XapI RAATTY 2 cut(s) 617, 1597
XceI RCATGY 2 cut(s) 349, 445
XmaI CCCGGG 2 cut(s) 129, 1456
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.