MD13G1222100.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013477
Physical Location & Seq
Forward (+)
21494717 .. 21496318
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1222100.v1.1.491

Sequence Viewer

Length: 1602 bp
ATGTCCCAATCAATCTTAGCTCCCTTCCAGCTTTTGGAACTCAACGTAATATCAGCGCAGGACCTTGCGCCCATGTCGCGTTCCATGCGCACATACGCCGTTGCATGGGTGCACCCGGATCGAAAACTCTCAACACGGGTGGACACCTCAGGCCACAACAACCCTACATGGAACGACAAGTTTGTGTTCCGTGTCGACGAGGACTTCTTGCATGAAGACACATCCGCGGTCATGATCGAGATCTACGCCCTTCATTGGTTCAAGGATGTTCACGTGGGAACCGTCCGTATCTTGGTTGGTAACCTTATTCCAACCCCGGCAAAGCCCCACCACCACCATCCTCAAGTGGGCATGCGTTTCGTGGCGCTCCAGGTGCGCCGCCCCTCGGGTCGGCCACAGGGGATTCTAAACATCGGCGTGGCGCTGCTTCATAGCTCTATGCGGAGCATGCCCTTGTATTCCCAACTCAGCGCCTCAGCAGTTGGATATAAAACTTTGATGGGCGAAGACGACGCGCCGCGCAGCACCAAAACCCACCACACCGGAAGCCAAATGACGACGTATTCGAAACCCGAGCTGAGGCGCACCAAGAGCGACTCCAGCTCAATGTTCGGGTCAGAGCTGAGAGAAAAGGAATTCAGACATAAGGGTAAAAAGGAAAAAGCAACTTCCGTGGTTAACTCGTCAGATGTCAGCGCTAGAAAAAGCAAGAAGGGTCGCGGTTCCAAAGCGAGCTCGTTGGTTGGCGGCTCATCAGATATAATTTACAGGAATGGCAAAAAGGGAAAAACAAGTTCTGTAGTCAGCGCGTCGGATGTTAGCTACCGAAAGGGGAAGGCGAGCTCCATGCTCAGCGCCTCGGATGTGGGCCCACCAAAGAGTTGGAAAGACAAAAAAGGGAAGCCAAGCTCCGCACTCAGCGCCTCCGATGTCCGCGCGCATGACCCACCGAAGAACGAGAGCAATGGCAACGAGAAACCGGCTAGCTTCGTCCTCAGCGCCTCCGAAGCGGAAGAACCGCCGAGAAAGATGCCTGCCAGTCACAAGCCCTCCCCGAAATTCACCCTGGTGGACAATTTTGGTGCTACCCCCGCGCGGAGGTCGGCTCTGCCAGCTGGCAAGTCGCCATTCCAGAGGCTGCAGTCGCCCTACAACGCGTATGCGACGCCAAGAAAGTCGAACCTCATGGCTGTTCCATTCCTAACGGAGTCGGAGCTGGGGCCGTCGCCGTCGGAGGTGGCTGCGGCGATAGCAAAGGAGCGAATGGACCAGGACAACGAGAGCTCAGTTGTGGTGGGTGCGTGGAACGAGGATGACAGCGTGGAGGGGTTGCAGTCGAAGCTAGAGCGGTGGCGGACGGAGCTCCCGCCAGTGTATGATAGGGGAGAGTTTTCGAGCTTTAAATCCAGTGAAACGCGCCACACACGGCGACACAGCGATGGGAGCAATGGGCTGTTCTCTTGCTTTAGTAACATATGTGGAATCGAGTGCTCCATCGTATGCGGGTCGGGCGACCAAGAAAAACCTCAGAGGAAGAACGGGAGTAAGAATGGCGGGCGGGTCCATCGGAGTCCATCGGTAGGAGATCTAAGCTACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

534

Amino Acids

58.32

Weight (kDa)

9.68

Isoelectric Point (pI)

54.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 11 - 108 1.1e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04540 AT2G33320
fragaria_vesca FvH4_4g04680
malus_domestica MD13G1222100.v1.1 MD16G1227000.v1.1
prunus_persica Prupe.1G049100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0395501
rosa_laevigata RLG00000009618
rosa_multiflora Rmu_sc0003334.1_g000002 Rmu_sc0020819.1_g000001
rosa_roxburghii Rroxscaffold_5G00340430
rosa_rugosa Rorug03G0355700
rosa_samantha Rh4AG060600 Rh4AG061800 Rh4BG059600 Rh4CG065300 Rh4DG055500
rosa_wichuraiana Rw4G005050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 34
AccBSI CCGCTC 1 cut(s) 1348
AccI GTMKAC 1 cut(s) 195
AclWI GGATC 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 2 cut(s) 635, 1058
AcvI CACGTG 1 cut(s) 274
AcyI GRCGYC 1 cut(s) 1166
AfeI AGCGCT 1 cut(s) 697
AflIII ACRYGT 1 cut(s) 1155
AgsI TTSAA 1 cut(s) 262
AjnI CCWGG 3 cut(s) 369, 1065, 1269
AleI CACNNNNGTG 1 cut(s) 1067
Alw21I GWGCWC 6 cut(s) 114, 737, 845, 1286, 1365, 1493
Alw44I GTGCAC 1 cut(s) 110
AlwI GGATC 1 cut(s) 126
AlwNI CAGNNNCTG 1 cut(s) 1138
Ama87I CYCGRG 2 cut(s) 385, 572
Aor51HI AGCGCT 1 cut(s) 697
AoxI GGCC 4 cut(s) 151, 392, 868, 1220
ApaI GGGCCC 1 cut(s) 872
ApaLI GTGCAC 1 cut(s) 110
ApeKI GCWGC 4 cut(s) 424, 522, 1138, 1241
ApoI RAATTY 2 cut(s) 635, 1058
ArsI GACNNNNNNTTYG 2 cut(s) 547, 579
AspS9I GGNCC 6 cut(s) 61, 868, 869, 1220, 1267, 1561
AsuC2I CCSGG 2 cut(s) 116, 317
AsuHPI GGTGA 1 cut(s) 1054
AsuII TTCGAA 1 cut(s) 566
AsuNHI GCTAGC 1 cut(s) 983
AvaI CYCGRG 2 cut(s) 385, 572
AvaII GGWCC 3 cut(s) 61, 1267, 1561
AxyI CCTNAGG 1 cut(s) 148
BaeGI GKGCMC 2 cut(s) 114, 872
BanII GRGCYC 5 cut(s) 737, 845, 872, 1286, 1365
BbrPI CACGTG 1 cut(s) 274
BbsI GAAGAC 2 cut(s) 222, 513
Bbv12I GWGCWC 6 cut(s) 114, 737, 845, 1286, 1365, 1493
BbvCI CCTCAGC 3 cut(s) 475, 578, 995
BbvI GCAGC 4 cut(s) 411, 534, 1125, 1228
BccI CCATC 6 cut(s) 345, 493, 1433, 1502, 1572, 1582
BceAI ACGGC 4 cut(s) 83, 1207, 1213, 1442
BcgI CGANNNNNNTGC 8 cut(s) 101, 135, 340, 374, 829, 863, 1012, 1046
BciT130I CCWGG 3 cut(s) 371, 1067, 1271
BcnI CCSGG 2 cut(s) 116, 317
BfaI CTAG 3 cut(s) 699, 984, 1343
BfmI CTRYAG 2 cut(s) 798, 1139
BfoI RGCGCY 7 cut(s) 368, 425, 474, 699, 858, 924, 1002
BglII AGATCT 2 cut(s) 240, 1585
BisI GCNGC 8 cut(s) 379, 425, 518, 523, 748, 1139, 1242, 1245
BlpI GCTNAGC 1 cut(s) 851
BlsI GCNGC 8 cut(s) 380, 426, 519, 524, 749, 1140, 1243, 1246
Bme1390I CCNGG 5 cut(s) 116, 317, 371, 1067, 1271
Bme18I GGWCC 3 cut(s) 61, 1267, 1561
BmeT110I CYCGRG 2 cut(s) 385, 572
BmgT120I GGNCC 6 cut(s) 61, 868, 869, 1220, 1267, 1561
BmiI GGNNCC 5 cut(s) 280, 724, 870, 1221, 1562
BmrFI CCNGG 5 cut(s) 116, 317, 371, 1067, 1271
BmsI GCATC 1 cut(s) 1020
BmtI GCTAGC 1 cut(s) 987
BpiI GAAGAC 2 cut(s) 222, 513
BplI GAGNNNNNCTC 2 cut(s) 1090, 1122
BpmI CTGGAG 2 cut(s) 353, 583
Bpu10I CCTNAGC 3 cut(s) 475, 578, 995
Bpu1102I GCTNAGC 1 cut(s) 851
Bpu14I TTCGAA 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 327
BpuMI CCSGG 2 cut(s) 116, 317
BsaAI YACGTR 2 cut(s) 274, 1597
BsaBI GATNNNNATC 1 cut(s) 239
BsaHI GRCGYC 1 cut(s) 1166
BsaJI CCNNGG 6 cut(s) 225, 315, 384, 672, 858, 1065
BsaWI WCCGGW 1 cut(s) 542
BsaXI ACNNNNNCTCC 2 cut(s) 1034, 1064
Bse118I RCCGGY 1 cut(s) 979
Bse1I ACTGG 3 cut(s) 1038, 1370, 1407
Bse21I CCTNAGG 1 cut(s) 148
Bse3DI GCAATG 2 cut(s) 970, 1453
Bse8I GATNNNNATC 1 cut(s) 239
BseBI CCWGG 3 cut(s) 371, 1067, 1271
BseDI CCNNGG 6 cut(s) 225, 315, 384, 672, 858, 1065
BseGI GGATG 6 cut(s) 221, 271, 337, 820, 868, 1318
BseJI GATNNNNATC 1 cut(s) 239
BseMI GCAATG 2 cut(s) 970, 1453
BseNI ACTGG 3 cut(s) 1038, 1370, 1407
BsePI GCGCGC 1 cut(s) 936
BseSI GKGCMC 2 cut(s) 114, 872
BseXI GCAGC 4 cut(s) 411, 534, 1125, 1228
BseYI CCCAGC 1 cut(s) 1216
BshFI GGCC 4 cut(s) 153, 394, 870, 1222
BsiHKAI GWGCWC 6 cut(s) 114, 737, 845, 1286, 1365, 1493
BsiHKCI CYCGRG 2 cut(s) 385, 572
BsiSI CCGG 4 cut(s) 116, 317, 543, 980
BsnI GGCC 4 cut(s) 153, 394, 870, 1222
BsoBI CYCGRG 2 cut(s) 385, 572
Bsp119I TTCGAA 1 cut(s) 566
Bsp120I GGGCCC 1 cut(s) 868
Bsp1286I GDGCHC 7 cut(s) 114, 737, 845, 872, 1286, 1365, 1493
Bsp143I GATC 4 cut(s) 118, 234, 240, 1585
Bsp1720I GCTNAGC 1 cut(s) 851
BspANI GGCC 4 cut(s) 153, 394, 870, 1222
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 5 cut(s) 280, 724, 870, 1221, 1562
BspMAI CTGCAG 1 cut(s) 1143
BspOI GCTAGC 1 cut(s) 987
BspPI GGATC 1 cut(s) 126
BspT104I TTCGAA 1 cut(s) 566
BsrBI CCGCTC 1 cut(s) 1348
BsrDI GCAATG 2 cut(s) 970, 1453
BsrFI RCCGGY 1 cut(s) 979
BsrI ACTGG 3 cut(s) 1038, 1370, 1407
BssAI RCCGGY 1 cut(s) 979
BssECI CCNNGG 6 cut(s) 225, 315, 384, 672, 858, 1065
BssHII GCGCGC 1 cut(s) 936
BssMI GATC 4 cut(s) 118, 234, 240, 1585
BssNI GRCGYC 1 cut(s) 1166
Bst2UI CCWGG 3 cut(s) 371, 1067, 1271
Bst4CI ACNGT 1 cut(s) 283
BstACI GRCGYC 1 cut(s) 1166
BstBAI YACGTR 2 cut(s) 274, 1597
BstBI TTCGAA 1 cut(s) 566
BstDSI CCRYGG 2 cut(s) 225, 672
BstEII GGTNACC 1 cut(s) 299
BstF5I GGATG 6 cut(s) 221, 271, 337, 820, 868, 1318
BstH2I RGCGCY 7 cut(s) 368, 425, 474, 699, 858, 924, 1002
BstKTI GATC 4 cut(s) 121, 237, 243, 1588
BstMBI GATC 4 cut(s) 118, 234, 240, 1585
BstNI CCWGG 3 cut(s) 371, 1067, 1271
BstNSI RCATGY 2 cut(s) 355, 451
BstPI GGTNACC 1 cut(s) 299
BstSCI CCNGG 5 cut(s) 114, 315, 369, 1065, 1269
BstSFI CTRYAG 2 cut(s) 798, 1139
BstSLI GKGCMC 2 cut(s) 114, 872
BstSNI TACGTA 1 cut(s) 1597
BstV1I GCAGC 4 cut(s) 411, 534, 1125, 1228
BstV2I GAAGAC 2 cut(s) 222, 513
BstX2I RGATCY 2 cut(s) 240, 1585
BstXI CCANNNNNNTGG 1 cut(s) 882
BstYI RGATCY 2 cut(s) 240, 1585
Bsu36I CCTNAGG 1 cut(s) 148
BsuRI GGCC 4 cut(s) 153, 394, 870, 1222
BtgI CCRYGG 2 cut(s) 225, 672
BtgZI GCGATG 1 cut(s) 1452
BtsCI GGATG 6 cut(s) 221, 271, 337, 820, 868, 1318
BtsIMutI CAGTG 2 cut(s) 1377, 1414
CaiI CAGNNNCTG 1 cut(s) 1138
CciI TCATGA 1 cut(s) 231
Cfr10I RCCGGY 1 cut(s) 979
Cfr13I GGNCC 6 cut(s) 61, 868, 869, 1220, 1267, 1561
Cfr42I CCGCGG 1 cut(s) 228
CseI GACGC 3 cut(s) 521, 798, 1174
CspCI CAANNNNNGTGG 4 cut(s) 120, 155, 654, 689
DpnI GATC 4 cut(s) 120, 236, 242, 1587
DpnII GATC 4 cut(s) 118, 234, 240, 1585
DraI TTTAAA 1 cut(s) 1402
EaeI YGGCCR 1 cut(s) 392
EciI GGCGGA 1 cut(s) 1369
Ecl136II GAGCTC 4 cut(s) 735, 843, 1284, 1363
Eco105I TACGTA 1 cut(s) 1597
Eco24I GRGCYC 5 cut(s) 737, 845, 872, 1286, 1365
Eco47I GGWCC 3 cut(s) 61, 1267, 1561
Eco47III AGCGCT 1 cut(s) 697
Eco53kI GAGCTC 4 cut(s) 735, 843, 1284, 1363
Eco72I CACGTG 1 cut(s) 274
Eco81I CCTNAGG 1 cut(s) 148
Eco88I CYCGRG 2 cut(s) 385, 572
Eco91I GGTNACC 1 cut(s) 299
EcoICRI GAGCTC 4 cut(s) 735, 843, 1284, 1363
EcoO109I RGGNCCY 1 cut(s) 61
EcoO65I GGTNACC 1 cut(s) 299
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 3 cut(s) 369, 1065, 1269
EcoT38I GRGCYC 5 cut(s) 737, 845, 872, 1286, 1365
FauI CCCGC 5 cut(s) 1099, 1374, 1496, 1547, 1551
FauNDI CATATG 1 cut(s) 1475
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 8 cut(s) 379, 425, 518, 523, 748, 1139, 1242, 1245
FokI GGATG 6 cut(s) 208, 278, 324, 827, 875, 1325
FriOI GRGCYC 5 cut(s) 737, 845, 872, 1286, 1365
Fsp4HI GCNGC 8 cut(s) 379, 425, 518, 523, 748, 1139, 1242, 1245
FspAI RTGCGCAY 1 cut(s) 89
FspBI CTAG 3 cut(s) 699, 984, 1343
FspI TGCGCA 1 cut(s) 89
GluI GCNGC 8 cut(s) 379, 425, 518, 523, 748, 1139, 1242, 1245
GsaI CCCAGC 1 cut(s) 1220
GsuI CTGGAG 2 cut(s) 353, 583
HaeII RGCGCY 7 cut(s) 368, 425, 474, 699, 858, 924, 1002
HaeIII GGCC 4 cut(s) 153, 394, 870, 1222
HapII CCGG 4 cut(s) 116, 317, 543, 980
HgaI GACGC 3 cut(s) 521, 798, 1174
Hin1I GRCGYC 1 cut(s) 1166
HincII GTYRAC 2 cut(s) 196, 679
HindII GTYRAC 2 cut(s) 196, 679
HinfI GANTC 5 cut(s) 403, 596, 1208, 1482, 1570
HpaI GTTAAC 1 cut(s) 679
HpaII CCGG 4 cut(s) 116, 317, 543, 980
HphI GGTGA 1 cut(s) 1054
Hpy166II GTNNAC 6 cut(s) 112, 142, 196, 271, 679, 1072
Hpy188III TCNNGA 3 cut(s) 232, 238, 1132
Hpy8I GTNNAC 6 cut(s) 112, 142, 196, 271, 679, 1072
Hpy99I CGWCG 7 cut(s) 200, 515, 562, 814, 1168, 1228, 1234
HpyAV CCTTC 4 cut(s) 34, 260, 706, 829
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 4 cut(s) 45, 273, 560, 1596
HpyCH4V TGCA 5 cut(s) 104, 112, 211, 1141, 1333
HpySE526I ACGT 4 cut(s) 45, 273, 560, 1596
Hsp92I GRCGYC 1 cut(s) 1166
KspAI GTTAAC 1 cut(s) 679
KspI CCGCGG 1 cut(s) 228
Kzo9I GATC 4 cut(s) 118, 234, 240, 1585
Lsp1109I GCAGC 4 cut(s) 411, 534, 1125, 1228
LweI GCATC 1 cut(s) 1020
MaeI CTAG 3 cut(s) 699, 984, 1343
MaeII ACGT 4 cut(s) 45, 273, 560, 1596
MaeIII GTNAC 3 cut(s) 299, 1040, 1469
MalI GATC 4 cut(s) 120, 236, 242, 1587
MbiI CCGCTC 1 cut(s) 1348
MboI GATC 4 cut(s) 118, 234, 240, 1585
MboII GAAGA 5 cut(s) 227, 518, 964, 1025, 1546
MflI RGATCY 2 cut(s) 240, 1585
MhlI GDGCHC 7 cut(s) 114, 737, 845, 872, 1286, 1365, 1493
MluCI AATT 4 cut(s) 635, 762, 1058, 1075
MluI ACGCGT 1 cut(s) 1155
MlyI GAGTC 3 cut(s) 590, 1217, 1579
MmeI TCCRAC 6 cut(s) 335, 463, 792, 863, 1191, 1212
MseI TTAA 2 cut(s) 678, 1401
MslI CAYNNNNRTG 3 cut(s) 416, 1067, 1437
MspA1I CMGCKG 2 cut(s) 227, 1115
MspI CCGG 4 cut(s) 116, 317, 543, 980
MspR9I CCNGG 5 cut(s) 116, 317, 371, 1067, 1271
MteI GCGCNGCGC 1 cut(s) 518
MvaI CCWGG 3 cut(s) 371, 1067, 1271
NciI CCSGG 2 cut(s) 116, 317
NdeI CATATG 1 cut(s) 1475
NdeII GATC 4 cut(s) 118, 234, 240, 1585
NheI GCTAGC 1 cut(s) 983
NlaIV GGNNCC 5 cut(s) 280, 724, 870, 1221, 1562
NmeAIII GCCGAG 1 cut(s) 1047
NmuCI GTSAC 1 cut(s) 1040
NsbI TGCGCA 1 cut(s) 89
NspI RCATGY 2 cut(s) 355, 451
NspV TTCGAA 1 cut(s) 566
OliI CACNNNNGTG 1 cut(s) 1067
PaeI GCATGC 2 cut(s) 355, 451
PagI TCATGA 1 cut(s) 231
PauI GCGCGC 1 cut(s) 936
PcsI WCGNNNNNNNCGW 2 cut(s) 279, 563
PfeI GAWTC 2 cut(s) 403, 1482
PflMI CCANNNNNTGG 1 cut(s) 34
PkrI GCNGC 8 cut(s) 380, 426, 519, 524, 749, 1140, 1243, 1246
PleI GAGTC 3 cut(s) 590, 1216, 1578
PmaCI CACGTG 1 cut(s) 274
PmlI CACGTG 1 cut(s) 274
PpsI GAGTC 3 cut(s) 590, 1216, 1578
Ppu21I YACGTR 2 cut(s) 274, 1597
PpuMI RGGWCCY 1 cut(s) 61
Psp124BI GAGCTC 4 cut(s) 737, 845, 1286, 1365
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 3 cut(s) 369, 1065, 1269
PspCI CACGTG 1 cut(s) 274
PspEI GGTNACC 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 1216
PspGI CCWGG 3 cut(s) 369, 1065, 1269
PspN4I GGNNCC 5 cut(s) 280, 724, 870, 1221, 1562
PspOMI GGGCCC 1 cut(s) 868
PspPI GGNCC 6 cut(s) 61, 868, 869, 1220, 1267, 1561
PspPPI RGGWCCY 1 cut(s) 61
PstI CTGCAG 1 cut(s) 1143
PstNI CAGNNNCTG 1 cut(s) 1138
PsuI RGATCY 2 cut(s) 240, 1585
PteI GCGCGC 1 cut(s) 936
PvuII CAGCTG 1 cut(s) 1115
RseI CAYNNNNRTG 3 cut(s) 416, 1067, 1437
SacI GAGCTC 4 cut(s) 737, 845, 1286, 1365
SacII CCGCGG 1 cut(s) 228
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 2 cut(s) 678, 1401
SatI GCNGC 8 cut(s) 379, 425, 518, 523, 748, 1139, 1242, 1245
Sau3AI GATC 4 cut(s) 118, 234, 240, 1585
Sau96I GGNCC 6 cut(s) 61, 868, 869, 1220, 1267, 1561
SchI GAGTC 3 cut(s) 590, 1217, 1579
ScrFI CCNGG 5 cut(s) 116, 317, 371, 1067, 1271
SduI GDGCHC 7 cut(s) 114, 737, 845, 872, 1286, 1365, 1493
SfaNI GCATC 1 cut(s) 1020
SfcI CTRYAG 2 cut(s) 798, 1139
Sfr303I CCGCGG 1 cut(s) 228
SfuI TTCGAA 1 cut(s) 566
SgrBI CCGCGG 1 cut(s) 228
SinI GGWCC 3 cut(s) 61, 1267, 1561
SmiMI CAYNNNNRTG 3 cut(s) 416, 1067, 1437
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
SnaBI TACGTA 1 cut(s) 1597
SphI GCATGC 2 cut(s) 355, 451
Sse9I AATT 4 cut(s) 635, 762, 1058, 1075
SspMI CTAG 3 cut(s) 699, 984, 1343
SstI GAGCTC 4 cut(s) 737, 845, 1286, 1365
StyD4I CCNGG 5 cut(s) 114, 315, 369, 1065, 1269
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 4 cut(s) 48, 276, 563, 1599
TaqI TCGA 8 cut(s) 121, 195, 237, 566, 1178, 1337, 1394, 1485
TasI AATT 4 cut(s) 635, 762, 1058, 1075
TauI GCSGC 4 cut(s) 381, 520, 750, 1247
TfiI GAWTC 2 cut(s) 403, 1482
Tru1I TTAA 2 cut(s) 678, 1401
Tru9I TTAA 2 cut(s) 678, 1401
TscAI CASTG 2 cut(s) 1377, 1414
TseFI GTSAC 1 cut(s) 1040
TseI GCWGC 4 cut(s) 424, 522, 1138, 1241
Tsp45I GTSAC 1 cut(s) 1040
TspDTI ATGAA 3 cut(s) 228, 242, 419
TspGWI ACGGA 5 cut(s) 179, 275, 661, 1220, 1373
TspRI CASTG 2 cut(s) 1377, 1414
Van91I CCANNNNNTGG 1 cut(s) 34
VneI GTGCAC 1 cut(s) 110
VpaK11BI GGWCC 3 cut(s) 61, 1267, 1561
XapI RAATTY 2 cut(s) 635, 1058
XceI RCATGY 2 cut(s) 355, 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 879
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 3 cut(s) 699, 984, 1343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.