Rorug03G0355700

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013477
Physical Location & Seq
Reverse (-)
43948200 .. 43953979
5780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0355700.1

Sequence Viewer

Length: 1080 bp
ATGGAGCAAGTCAAACAACCCGGAGCTAAGACCTATGCCATCGATCCGGCTATGGCTGAAAGTTTGATCACCCGACTTGAGAGTGAGTTATTGCATATGAGGCAGGGTGACATGACGGTGTTGGAATATGGGCGACGTTTCAATAGCTTGTATCGTTTTGCACCTGCCTTGGTGGCTAATGAGAGGGATAAAATCCACAAATTTCTACAAGGACTACGCGATCAACTCCGTGTTATGTTAGCGGCTACCTTATTTACCGAGTTCAGTCAAGTATTTTTGGCCGCCATGAAGTGCGAGGACAGACTTTTGAAATGTGTACAACCATTAGAGGCGGGTTCCCCTAGCCCAAGTCCATCCAAGAGGGCTACCGCTATTTCGAGGTCCGAGGCTTTGACCGGTAGTAGACCTGACAGTTTTGGGTCCGACTCACGACAGAGTTACAGAAGACGTGAACGGAGGCGGGGCAAGCACTATAAGCGACAAACCAAACGGGCCAGGAGTAGTGTTAGCGGTTATAGTAGAGGTTCTAGAAGACTTCCCATTCAGAGAGACCGTCCACAATGTCAGACGTGTGGCAGGCACCATGATGGACAGTGCCAAGTAGGACTATACACGATAGGGAGTGTGGTTGAGATCTATGAGGATAACGATAATGCATGCGAGCAAGGACGAGTTTGCGGCTCTGCTCTCAGATCGATGACCAATATATCCATGAGTACCATAGGAGACATCCAGCTGCACGTACTAGTTGCTGATGCCAATGAGGAACATGAAGCACTGCTCAAACAGGAGCCTCAAGTGGAGAGAATGTTGTGGAGCACTGTGAAGTCCTTCCTTGATCCCAAGACAACTGAAAGGATTAATGTCCTTGGTGACAAGAGCAAGTTACTTCAAATAATTGATGCTAGATGGCTTTTCTTGGCATGTCACCCTCGCTTGTGGCTGCGAGTTGAACGATCCATTAAAGATCCATTGGAGCCTGGCGTCTTCCCCAGTATTGAGACAGCGGTTACTGCCGCAAGGCTTGGCGACACCATTCTAATTTCAGCAGGGGGAAAGCATCTTGCTTCTGATACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

359

Amino Acids

40.44

Weight (kDa)

9.41

Isoelectric Point (pI)

65.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 24 - 74 8.4e-09 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04540 AT2G33320
fragaria_vesca FvH4_4g04680
malus_domestica MD13G1222100.v1.1 MD16G1227000.v1.1
prunus_persica Prupe.1G049100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0395501
rosa_laevigata RLG00000009618
rosa_multiflora Rmu_sc0003334.1_g000002 Rmu_sc0020819.1_g000001
rosa_roxburghii Rroxscaffold_5G00340430
rosa_rugosa Rorug03G0355700
rosa_samantha Rh4AG060600 Rh4AG061800 Rh4BG059600 Rh4CG065300 Rh4DG055500
rosa_wichuraiana Rw4G005050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
Acc36I ACCTGC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 579
AccI GTMKAC 1 cut(s) 403
AccII CGCG 1 cut(s) 219
AciI CCGC 9 cut(s) 242, 282, 332, 369, 460, 510, 678, 1007, 1017
AclWI GGATC 4 cut(s) 38, 833, 951, 962
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 200
AcyI GRCGYC 1 cut(s) 984
AfaI GTAC 3 cut(s) 318, 718, 744
AflIII ACRYGT 1 cut(s) 569
AgeI ACCGGT 1 cut(s) 395
AgsI TTSAA 4 cut(s) 142, 310, 893, 953
AhlI ACTAGT 1 cut(s) 745
AjiI CACGTC 2 cut(s) 449, 570
AjnI CCWGG 2 cut(s) 494, 979
AluBI AGCT 3 cut(s) 26, 147, 736
AluI AGCT 3 cut(s) 26, 147, 736
Alw21I GWGCWC 1 cut(s) 821
Alw26I GTCTC 3 cut(s) 543, 720, 995
AlwI GGATC 4 cut(s) 38, 833, 951, 962
AoxI GGCC 2 cut(s) 279, 492
ApeKI GCWGC 2 cut(s) 736, 943
ApoI RAATTY 1 cut(s) 200
AseI ATTAAT 1 cut(s) 861
AsiGI ACCGGT 1 cut(s) 395
Asp700I GAANNNNTTC 1 cut(s) 830
AspS9I GGNCC 3 cut(s) 381, 420, 492
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 4 cut(s) 61, 119, 884, 920
AvaII GGWCC 2 cut(s) 381, 420
BanI GGYRCC 1 cut(s) 579
BbsI GAAGAC 3 cut(s) 451, 538, 979
Bbv12I GWGCWC 1 cut(s) 821
BbvI GCAGC 2 cut(s) 723, 930
BccI CCATC 4 cut(s) 47, 361, 581, 903
BciT130I CCWGG 2 cut(s) 496, 981
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 3 cut(s) 543, 720, 995
BcuI ACTAGT 1 cut(s) 745
BfaI CTAG 4 cut(s) 342, 528, 746, 906
BfuAI ACCTGC 1 cut(s) 172
BglI GCCNNNNNGGC 1 cut(s) 173
BglII AGATCT 1 cut(s) 633
BisI GCNGC 6 cut(s) 243, 282, 679, 737, 944, 1017
BlsI GCNGC 6 cut(s) 244, 283, 680, 738, 945, 1018
Bme1390I CCNGG 3 cut(s) 21, 496, 981
Bme18I GGWCC 2 cut(s) 381, 420
BmgBI CACGTC 2 cut(s) 449, 570
BmgT120I GGNCC 3 cut(s) 381, 420, 492
BmiI GGNNCC 5 cut(s) 337, 421, 581, 792, 978
BmrFI CCNGG 3 cut(s) 21, 496, 981
BmrI ACTGGG 1 cut(s) 987
BmsI GCATC 3 cut(s) 745, 892, 1069
BmuI ACTGGG 1 cut(s) 987
BpiI GAAGAC 3 cut(s) 451, 538, 979
BpuEI CTTGAG 2 cut(s) 98, 780
BpuMI CCSGG 1 cut(s) 21
Bsa29I ATCGAT 2 cut(s) 42, 695
BsaAI YACGTR 1 cut(s) 742
BsaHI GRCGYC 1 cut(s) 984
BsaI GGTCTC 1 cut(s) 543
BsaJI CCNNGG 3 cut(s) 168, 384, 868
BsaWI WCCGGW 1 cut(s) 395
BsaXI ACNNNNNCTCC 2 cut(s) 794, 824
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 993
BseBI CCWGG 2 cut(s) 496, 981
BseCI ATCGAT 2 cut(s) 42, 695
BseDI CCNNGG 3 cut(s) 168, 384, 868
BseGI GGATG 2 cut(s) 353, 729
BseMII CTCAG 1 cut(s) 703
BseNI ACTGG 1 cut(s) 993
BseXI GCAGC 2 cut(s) 723, 930
BsgI GTGCAG 1 cut(s) 722
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 281, 494
BshNI GGYRCC 1 cut(s) 579
BshTI ACCGGT 1 cut(s) 395
BshVI ATCGAT 2 cut(s) 42, 695
BsiHKAI GWGCWC 1 cut(s) 821
BsiSI CCGG 3 cut(s) 21, 47, 396
BsmAI GTCTC 3 cut(s) 543, 720, 995
BsnI GGCC 2 cut(s) 281, 494
Bso31I GGTCTC 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 821
Bsp1407I TGTACA 1 cut(s) 316
Bsp143I GATC 8 cut(s) 43, 66, 220, 633, 692, 838, 956, 967
BspACI CCGC 9 cut(s) 242, 282, 332, 369, 460, 510, 678, 1007, 1017
BspANI GGCC 2 cut(s) 281, 494
BspCNI CTCAG 1 cut(s) 702
BspDI ATCGAT 2 cut(s) 42, 695
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 5 cut(s) 337, 421, 581, 792, 978
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 4 cut(s) 38, 833, 951, 962
BspT107I GGYRCC 1 cut(s) 579
BspTNI GGTCTC 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 395
BsrGI TGTACA 1 cut(s) 316
BsrI ACTGG 1 cut(s) 993
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 3 cut(s) 168, 384, 868
BssMI GATC 8 cut(s) 43, 66, 220, 633, 692, 838, 956, 967
BssNI GRCGYC 1 cut(s) 984
BssT1I CCWWGG 2 cut(s) 168, 868
Bst2UI CCWGG 2 cut(s) 496, 981
Bst4CI ACNGT 5 cut(s) 118, 413, 554, 594, 823
BstACI GRCGYC 1 cut(s) 984
BstAUI TGTACA 1 cut(s) 316
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 4 cut(s) 467, 578, 658, 662
BstDEI CTNAG 2 cut(s) 27, 689
BstF5I GGATG 2 cut(s) 353, 729
BstFNI CGCG 1 cut(s) 219
BstKTI GATC 8 cut(s) 46, 69, 223, 636, 695, 841, 959, 970
BstMAI GTCTC 3 cut(s) 543, 720, 995
BstMBI GATC 8 cut(s) 43, 66, 220, 633, 692, 838, 956, 967
BstMWI GCNNNNNNNGC 5 cut(s) 100, 173, 466, 475, 1013
BstNI CCWGG 2 cut(s) 496, 981
BstNSI RCATGY 2 cut(s) 660, 927
BstSCI CCNGG 3 cut(s) 19, 494, 979
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 723, 930
BstV2I GAAGAC 3 cut(s) 451, 538, 979
BstX2I RGATCY 2 cut(s) 633, 967
BstYI RGATCY 2 cut(s) 633, 967
Bsu15I ATCGAT 2 cut(s) 42, 695
BsuRI GGCC 2 cut(s) 281, 494
BsuTUI ATCGAT 2 cut(s) 42, 695
BtrI CACGTC 2 cut(s) 449, 570
BtsCI GGATG 2 cut(s) 353, 729
BtsI GCAGTG 1 cut(s) 776
BtsIMutI CAGTG 3 cut(s) 599, 776, 819
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 4 cut(s) 467, 578, 658, 662
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 3 cut(s) 381, 420, 492
ClaI ATCGAT 2 cut(s) 42, 695
CseI GACGC 1 cut(s) 973
Csp6I GTAC 3 cut(s) 317, 717, 743
CspAI ACCGGT 1 cut(s) 395
CviAII CATG 7 cut(s) 112, 286, 584, 657, 712, 770, 924
CviQI GTAC 3 cut(s) 317, 717, 743
DdeI CTNAG 2 cut(s) 27, 689
DpnI GATC 8 cut(s) 45, 68, 222, 635, 694, 840, 958, 969
DpnII GATC 8 cut(s) 43, 66, 220, 633, 692, 838, 956, 967
EaeI YGGCCR 1 cut(s) 279
Eco130I CCWWGG 2 cut(s) 168, 868
Eco31I GGTCTC 1 cut(s) 543
Eco47I GGWCC 2 cut(s) 381, 420
EcoRII CCWGG 2 cut(s) 494, 979
EcoT14I CCWWGG 2 cut(s) 168, 868
EcoT22I ATGCAT 1 cut(s) 658
ErhI CCWWGG 2 cut(s) 168, 868
FaeI CATG 7 cut(s) 115, 289, 587, 660, 715, 773, 927
FatI CATG 7 cut(s) 111, 285, 583, 656, 711, 769, 923
FauI CCCGC 2 cut(s) 325, 453
FauNDI CATATG 1 cut(s) 96
FbaI TGATCA 1 cut(s) 66
FblI GTMKAC 1 cut(s) 403
Fnu4HI GCNGC 6 cut(s) 243, 282, 679, 737, 944, 1017
FokI GGATG 2 cut(s) 340, 716
Fsp4HI GCNGC 6 cut(s) 243, 282, 679, 737, 944, 1017
FspBI CTAG 4 cut(s) 342, 528, 746, 906
GluI GCNGC 6 cut(s) 243, 282, 679, 737, 944, 1017
HaeIII GGCC 2 cut(s) 281, 494
HapII CCGG 3 cut(s) 21, 47, 396
HgaI GACGC 1 cut(s) 973
Hin1I GRCGYC 1 cut(s) 984
Hin1II CATG 7 cut(s) 115, 289, 587, 660, 715, 773, 927
HinfI GANTC 1 cut(s) 425
HpaII CCGG 3 cut(s) 21, 47, 396
HphI GGTGA 4 cut(s) 61, 119, 884, 920
Hpy166II GTNNAC 4 cut(s) 317, 404, 452, 557
Hpy188I TCNGA 6 cut(s) 385, 424, 546, 567, 692, 1072
Hpy188III TCNNGA 2 cut(s) 429, 528
Hpy8I GTNNAC 4 cut(s) 317, 404, 452, 557
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 1 cut(s) 841
HpyCH4III ACNGT 5 cut(s) 118, 413, 554, 594, 823
HpyCH4IV ACGT 4 cut(s) 136, 448, 569, 741
HpyCH4V TGCA 4 cut(s) 94, 161, 656, 739
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 173, 466, 475, 1013
HpyF3I CTNAG 2 cut(s) 27, 689
HpySE526I ACGT 4 cut(s) 136, 448, 569, 741
Hsp92I GRCGYC 1 cut(s) 984
Hsp92II CATG 7 cut(s) 115, 289, 587, 660, 715, 773, 927
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 8 cut(s) 43, 66, 220, 633, 692, 838, 956, 967
LmnI GCTCC 5 cut(s) 4, 23, 790, 816, 976
Lsp1109I GCAGC 2 cut(s) 723, 930
LweI GCATC 3 cut(s) 745, 892, 1069
MaeI CTAG 4 cut(s) 342, 528, 746, 906
MaeII ACGT 4 cut(s) 136, 448, 569, 741
MaeIII GTNAC 6 cut(s) 107, 437, 872, 885, 926, 1009
MalI GATC 8 cut(s) 45, 68, 222, 635, 694, 840, 958, 969
MboI GATC 8 cut(s) 43, 66, 220, 633, 692, 838, 956, 967
MboII GAAGA 3 cut(s) 456, 543, 979
MflI RGATCY 2 cut(s) 633, 967
MhlI GDGCHC 1 cut(s) 821
MluCI AATT 3 cut(s) 200, 897, 1041
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 2 cut(s) 102, 447
Mph1103I ATGCAT 1 cut(s) 658
MroXI GAANNNNTTC 1 cut(s) 830
MseI TTAA 3 cut(s) 861, 963, 1078
MslI CAYNNNNRTG 2 cut(s) 116, 585
MspA1I CMGCKG 2 cut(s) 736, 1007
MspI CCGG 3 cut(s) 21, 47, 396
MspR9I CCNGG 3 cut(s) 21, 496, 981
MvaI CCWGG 2 cut(s) 496, 981
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 5 cut(s) 100, 173, 466, 475, 1013
NciI CCSGG 1 cut(s) 21
NdeI CATATG 1 cut(s) 96
NdeII GATC 8 cut(s) 43, 66, 220, 633, 692, 838, 956, 967
NlaIII CATG 7 cut(s) 115, 289, 587, 660, 715, 773, 927
NlaIV GGNNCC 5 cut(s) 337, 421, 581, 792, 978
NmuCI GTSAC 3 cut(s) 107, 872, 926
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 2 cut(s) 660, 927
PaeI GCATGC 1 cut(s) 660
PaqCI CACCTGC 1 cut(s) 172
PdmI GAANNNNTTC 1 cut(s) 830
PinAI ACCGGT 1 cut(s) 395
PkrI GCNGC 6 cut(s) 244, 283, 680, 738, 945, 1018
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
Ppu21I YACGTR 1 cut(s) 742
PshBI ATTAAT 1 cut(s) 861
Psp6I CCWGG 2 cut(s) 494, 979
PspGI CCWGG 2 cut(s) 494, 979
PspN4I GGNNCC 5 cut(s) 337, 421, 581, 792, 978
PspPI GGNCC 3 cut(s) 381, 420, 492
PsuI RGATCY 2 cut(s) 633, 967
PvuII CAGCTG 1 cut(s) 736
RsaI GTAC 3 cut(s) 318, 718, 744
RsaNI GTAC 3 cut(s) 317, 717, 743
RseI CAYNNNNRTG 2 cut(s) 116, 585
SaqAI TTAA 3 cut(s) 861, 963, 1078
SatI GCNGC 6 cut(s) 243, 282, 679, 737, 944, 1017
Sau3AI GATC 8 cut(s) 43, 66, 220, 633, 692, 838, 956, 967
Sau96I GGNCC 3 cut(s) 381, 420, 492
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 3 cut(s) 21, 496, 981
SduI GDGCHC 1 cut(s) 821
SfaNI GCATC 3 cut(s) 745, 892, 1069
SinI GGWCC 2 cut(s) 381, 420
SmiMI CAYNNNNRTG 2 cut(s) 116, 585
SmlI CTYRAG 2 cut(s) 77, 795
SmoI CTYRAG 2 cut(s) 77, 795
SpeI ACTAGT 1 cut(s) 745
SphI GCATGC 1 cut(s) 660
Sse9I AATT 3 cut(s) 200, 897, 1041
SsiI CCGC 9 cut(s) 242, 282, 332, 369, 460, 510, 678, 1007, 1017
SspMI CTAG 4 cut(s) 342, 528, 746, 906
StyD4I CCNGG 3 cut(s) 19, 494, 979
StyI CCWWGG 2 cut(s) 168, 868
TaaI ACNGT 5 cut(s) 118, 413, 554, 594, 823
TaiI ACGT 4 cut(s) 139, 451, 572, 744
TaqI TCGA 3 cut(s) 42, 377, 695
TasI AATT 3 cut(s) 200, 897, 1041
TatI WGTACW 1 cut(s) 316
TauI GCSGC 4 cut(s) 245, 284, 681, 1019
Tru1I TTAA 3 cut(s) 861, 963, 1078
Tru9I TTAA 3 cut(s) 861, 963, 1078
TscAI CASTG 3 cut(s) 599, 783, 826
TseFI GTSAC 3 cut(s) 107, 872, 926
TseI GCWGC 2 cut(s) 736, 943
Tsp45I GTSAC 3 cut(s) 107, 872, 926
TspDTI ATGAA 2 cut(s) 302, 786
TspGWI ACGGA 2 cut(s) 218, 469
TspRI CASTG 3 cut(s) 599, 783, 826
VpaK11BI GGWCC 2 cut(s) 381, 420
VspI ATTAAT 1 cut(s) 861
XapI RAATTY 1 cut(s) 200
XbaI TCTAGA 1 cut(s) 527
XceI RCATGY 2 cut(s) 660, 927
XmiI GTMKAC 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 830
XspI CTAG 4 cut(s) 342, 528, 746, 906
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.