Prupe.1G049100_v2.0.a1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013477
Physical Location & Seq
Forward (+)
3447741 .. 3450097
2357 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G049100.1

Sequence Viewer

Length: 1620 bp
ATGTCTCAATCAATCTTAGCTCCGTTCCAACTCCTGGAACTCAACGTCATATCAGCCCAGGACCTTGCGCCGATATCACGCTCCATGCGCACGTACGCCATTGCATGGGTGCACCCAGATCGAAAGCTATCGACGCGGGTGGACACTCAGGGCCACAACAATCCGACATGGAACGACAAGTTTGTGTTCCGGGTGGACGAGGATTTTTTACACGAGGACACCTCCGCCGTGATGATTGAGATCTACGCTCTCCATTGGTTCAAGGACGTCCATGTGGGCACGGTTCGTGTCCTGGTGGGTAACCTTATCCCCACCCCGGCAAAGCCCCACCACCACAACCCTCATCTAGGCATGCGGTTCGTAGCTCTCCAGGTGCGCCGCCCCTCGGGCCGGCCGCAGGGGATTCTAAACATCGGCGTGGCGCTACTTCACAGCTCCATGAGGAGCATGCCCTTGTATTCCCAACTCAGCACATCAGCCGTCGGATACAAAACCTTGATGGGCGAGGAAGACCCACATCGCAGTAGTCAAACCCATCATGCAGGCAGTCAAGCGACGACGTATTCGAAGCCGGAGCTACGGCGCAGCAAGAGCGACTCCAGCTCCATGTTCGGGTCGGAGCTGAGAGCCACAGAATTTAAAAACAAAGGCAAAAATGGAAAAGCGGGCTCAATGGTCAACGGGTCAGAAGTCAGCATAAAAAAGAACAAAAAAGGTCGTCTGTCCAAAGCCAGCTCGATCATTGGTGGTTCAGAAATAATTATCAGGAAGAGCAAGGACAAAAAAGGAAAATCAAGCTCCCTGCTTAGCGCCTCAGATATGAGCTACAAAAAAGGGAAGCCGAGCTCCCTACTCGGCGCCTCAGACGTGAACAGCTCGGTGGCCCCACCAAAGTACGGAAAAGACAGAAAAGGGAAGCCGAGCTCCGTTCTCAGCGCCTCCGAAGTCAGCGTGCAGGACCCACCGAAAAAGGGTAATAGTAGTAACGGTAAACCCGCAAGCTCCATGCCCAGCGCATCCGAACCGGAAGACAGGCCCAGAAGAAAACCCGCCAGTCACAAACCATCGCCGAAATTCAACCTGGCAGAAAATTACGGTTCTACCCCCGGACGGAAGTCGGCGCCGCGAGCTGGCAAATCGCCGTTCTACAAGCTGCAGTCACCGTACGAGCAGTACGCGACGCCGAGGAAATCGAACATCATGCCGGTGCCGTTCATAACGGAGTCGGAGCTGGGGCCGTCGCCGTCGGAGGTGGCGGCGGCGATCGCGAAGGAGCGCTTGGATCAGGACGCGGAGAGCTCGGTGGTGGTGGGCGCGTGGAACGAGGAAGACAGCGTGGAGGGCTTGCAGTCCAAGCTCGAGCGGTGGCGCACGGAGCTGCCGCCAGTTTATGATCGCGGAGAGTTCTCGAGCTTTCCTTCCAGCGACGAGCGCCACGAGCGGCGGCACAGCGACGGCGGAAGCGGGCTCTTCTCGTGCTTTAGTAATATTTGTGGAATCGAGTGCTCCATTGTATGCGGGTCGGGCGATTCGGATAGTAAGACCCGGAAGAAGAATGGTAGCAAGAGTGGCGGAAAGGTCCCACGGAGCCCCTCAGCCGGAAATCTAAGCTACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

540

Amino Acids

58.67

Weight (kDa)

9.7

Isoelectric Point (pI)

62.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04540 AT2G33320
fragaria_vesca FvH4_4g04680
malus_domestica MD13G1222100.v1.1 MD16G1227000.v1.1
prunus_persica Prupe.1G049100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0395501
rosa_laevigata RLG00000009618
rosa_multiflora Rmu_sc0003334.1_g000002 Rmu_sc0020819.1_g000001
rosa_roxburghii Rroxscaffold_5G00340430
rosa_rugosa Rorug03G0355700
rosa_samantha Rh4AG060600 Rh4AG061800 Rh4BG059600 Rh4CG065300 Rh4DG055500
rosa_wichuraiana Rw4G005050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
Acc16I TGCGCA 1 cut(s) 89
AccB1I GGYRCC 3 cut(s) 857, 1120, 1207
AccB7I CCANNNNNTGG 2 cut(s) 34, 105
AccBSI CCGCTC 2 cut(s) 1363, 1441
AccII CGCG 7 cut(s) 136, 1126, 1178, 1268, 1292, 1316, 1398
AclWI GGATC 1 cut(s) 1290
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 2 cut(s) 635, 1073
AcyI GRCGYC 4 cut(s) 267, 858, 1121, 1181
AfaI GTAC 4 cut(s) 95, 896, 1166, 1175
AfeI AGCGCT 1 cut(s) 1277
AflIII ACRYGT 1 cut(s) 1614
AgsI TTSAA 2 cut(s) 262, 1078
AjiI CACGTC 1 cut(s) 868
AjnI CCWGG 5 cut(s) 33, 57, 291, 369, 1080
AjuI GAANNNNNNNTTGG 2 cut(s) 1262, 1294
Alw21I GWGCWC 5 cut(s) 114, 848, 926, 1301, 1508
Alw26I GTCTC 1 cut(s) 9
Alw44I GTGCAC 1 cut(s) 110
AlwI GGATC 1 cut(s) 1290
Ama87I CYCGRG 3 cut(s) 385, 1358, 1408
Aor51HI AGCGCT 1 cut(s) 1277
AoxI GGCC 6 cut(s) 151, 388, 392, 882, 1034, 1235
ApaLI GTGCAC 1 cut(s) 110
ApeKI GCWGC 3 cut(s) 585, 1153, 1378
ApoI RAATTY 2 cut(s) 635, 1073
ArsI GACNNNNNNTTYG 2 cut(s) 547, 579
AsiSI GCGATCGC 1 cut(s) 1266
AspS9I GGNCC 8 cut(s) 61, 151, 388, 883, 958, 1035, 1235, 1579
AsuC2I CCSGG 4 cut(s) 191, 317, 1107, 1546
AsuHPI GGTGA 1 cut(s) 1152
AsuII TTCGAA 1 cut(s) 566
AvaI CYCGRG 3 cut(s) 385, 1358, 1408
AvaII GGWCC 3 cut(s) 61, 958, 1579
BaeGI GKGCMC 2 cut(s) 114, 281
BanI GGYRCC 3 cut(s) 857, 1120, 1207
BanII GRGCYC 6 cut(s) 671, 848, 926, 1301, 1470, 1592
BauI CACGAG 3 cut(s) 212, 1436, 1474
BbsI GAAGAC 3 cut(s) 516, 1035, 1335
Bbv12I GWGCWC 5 cut(s) 114, 848, 926, 1301, 1508
BbvCI CCTCAGC 1 cut(s) 1594
BbvI GCAGC 3 cut(s) 597, 1140, 1365
BccI CCATC 3 cut(s) 493, 543, 1072
BceAI ACGGC 8 cut(s) 212, 464, 596, 1126, 1195, 1222, 1228, 1471
BcgI CGANNNNNNTGC 6 cut(s) 101, 135, 340, 374, 1183, 1217
BciT130I CCWGG 5 cut(s) 35, 59, 293, 371, 1082
BciVI GTATCC 1 cut(s) 479
BcnI CCSGG 4 cut(s) 191, 317, 1107, 1546
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 347
BfmI CTRYAG 1 cut(s) 1154
BfoI RGCGCY 7 cut(s) 425, 813, 861, 939, 1124, 1279, 1435
BfuI GTATCC 1 cut(s) 479
BglI GCCNNNNNGGC 1 cut(s) 387
BglII AGATCT 1 cut(s) 240
BlpI GCTNAGC 1 cut(s) 806
Bme1390I CCNGG 9 cut(s) 35, 59, 191, 293, 317, 371, 1082, 1107, 1546
Bme18I GGWCC 3 cut(s) 61, 958, 1579
BmeT110I CYCGRG 3 cut(s) 385, 1358, 1408
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 8 cut(s) 61, 151, 388, 883, 958, 1035, 1235, 1579
BmiI GGNNCC 8 cut(s) 859, 885, 960, 1122, 1209, 1236, 1581, 1589
BmrFI CCNGG 9 cut(s) 35, 59, 191, 293, 317, 371, 1082, 1107, 1546
BmsI GCATC 1 cut(s) 1025
BoxI GACNNNNGTC 1 cut(s) 1114
BpiI GAAGAC 3 cut(s) 516, 1035, 1335
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BpmI CTGGAG 2 cut(s) 353, 583
Bpu10I CCTNAGC 1 cut(s) 1594
Bpu1102I GCTNAGC 1 cut(s) 806
Bpu14I TTCGAA 1 cut(s) 566
BpuMI CCSGG 4 cut(s) 191, 317, 1107, 1546
BsaAI YACGTR 1 cut(s) 93
BsaBI GATNNNNATC 1 cut(s) 239
BsaHI GRCGYC 4 cut(s) 267, 858, 1121, 1181
BsaJI CCNNGG 6 cut(s) 57, 315, 384, 1105, 1184, 1583
BsaWI WCCGGW 1 cut(s) 1024
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bse118I RCCGGY 2 cut(s) 390, 1204
Bse1I ACTGG 2 cut(s) 1053, 1385
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 239
BseBI CCWGG 5 cut(s) 35, 59, 293, 371, 1082
BseDI CCNNGG 6 cut(s) 57, 315, 384, 1105, 1184, 1583
BseGI GGATG 1 cut(s) 1016
BseJI GATNNNNATC 1 cut(s) 239
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 7 cut(s) 161, 481, 614, 828, 876, 946, 1608
BseNI ACTGG 2 cut(s) 1053, 1385
BseRI GAGGAG 1 cut(s) 457
BseSI GKGCMC 2 cut(s) 114, 281
BseX3I CGGCCG 1 cut(s) 392
BseXI GCAGC 3 cut(s) 597, 1140, 1365
BseYI CCCAGC 2 cut(s) 1010, 1231
BsgI GTGCAG 1 cut(s) 974
Bsh1236I CGCG 7 cut(s) 136, 1126, 1178, 1268, 1292, 1316, 1398
Bsh1285I CGRYCG 2 cut(s) 395, 1266
BshFI GGCC 6 cut(s) 153, 390, 394, 884, 1036, 1237
BshNI GGYRCC 3 cut(s) 857, 1120, 1207
BsiEI CGRYCG 2 cut(s) 395, 1266
BsiHKAI GWGCWC 5 cut(s) 114, 848, 926, 1301, 1508
BsiHKCI CYCGRG 3 cut(s) 385, 1358, 1408
BsiSI CCGG 9 cut(s) 190, 317, 391, 572, 1025, 1107, 1205, 1546, 1599
BsiWI CGTACG 2 cut(s) 93, 1164
BslFI GGGAC 1 cut(s) 1565
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 1565
BsnI GGCC 6 cut(s) 153, 390, 394, 884, 1036, 1237
BsoBI CYCGRG 3 cut(s) 385, 1358, 1408
Bsp119I TTCGAA 1 cut(s) 566
Bsp1286I GDGCHC 9 cut(s) 114, 281, 671, 848, 926, 1301, 1470, 1508, 1592
Bsp143I GATC 6 cut(s) 118, 240, 738, 1263, 1282, 1393
Bsp1720I GCTNAGC 1 cut(s) 806
Bsp68I TCGCGA 1 cut(s) 1268
BspANI GGCC 6 cut(s) 153, 390, 394, 884, 1036, 1237
BspCNI CTCAG 7 cut(s) 160, 480, 615, 827, 875, 945, 1607
BspFNI CGCG 7 cut(s) 136, 1126, 1178, 1268, 1292, 1316, 1398
BspLI GGNNCC 8 cut(s) 859, 885, 960, 1122, 1209, 1236, 1581, 1589
BspMAI CTGCAG 1 cut(s) 1158
BspPI GGATC 1 cut(s) 1290
BspQI GCTCTTC 2 cut(s) 764, 1475
BspT104I TTCGAA 1 cut(s) 566
BspT107I GGYRCC 3 cut(s) 857, 1120, 1207
BsrBI CCGCTC 2 cut(s) 1363, 1441
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 2 cut(s) 390, 1204
BsrI ACTGG 2 cut(s) 1053, 1385
BssAI RCCGGY 2 cut(s) 390, 1204
BssECI CCNNGG 6 cut(s) 57, 315, 384, 1105, 1184, 1583
BssMI GATC 6 cut(s) 118, 240, 738, 1263, 1282, 1393
BssNI GRCGYC 4 cut(s) 267, 858, 1121, 1181
BssSI CACGAG 3 cut(s) 212, 1436, 1474
Bst2BI CACGAG 3 cut(s) 212, 1436, 1474
Bst2UI CCWGG 5 cut(s) 35, 59, 293, 371, 1082
Bst4CI ACNGT 4 cut(s) 283, 989, 1097, 1164
Bst6I CTCTTC 2 cut(s) 764, 1475
BstACI GRCGYC 4 cut(s) 267, 858, 1121, 1181
BstBAI YACGTR 1 cut(s) 93
BstBI TTCGAA 1 cut(s) 566
BstDSI CCRYGG 1 cut(s) 1583
BstEII GGTNACC 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 345
BstF5I GGATG 1 cut(s) 1016
BstFNI CGCG 7 cut(s) 136, 1126, 1178, 1268, 1292, 1316, 1398
BstH2I RGCGCY 7 cut(s) 425, 813, 861, 939, 1124, 1279, 1435
BstKTI GATC 6 cut(s) 121, 243, 741, 1266, 1285, 1396
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 6 cut(s) 118, 240, 738, 1263, 1282, 1393
BstMCI CGRYCG 2 cut(s) 395, 1266
BstNI CCWGG 5 cut(s) 35, 59, 293, 371, 1082
BstNSI RCATGY 3 cut(s) 355, 451, 1618
BstPAI GACNNNNGTC 1 cut(s) 1114
BstPI GGTNACC 1 cut(s) 299
BstSCI CCNGG 9 cut(s) 33, 57, 189, 291, 315, 369, 1080, 1105, 1544
BstSFI CTRYAG 1 cut(s) 1154
BstSLI GKGCMC 2 cut(s) 114, 281
BstUI CGCG 7 cut(s) 136, 1126, 1178, 1268, 1292, 1316, 1398
BstV1I GCAGC 3 cut(s) 597, 1140, 1365
BstV2I GAAGAC 3 cut(s) 516, 1035, 1335
BstX2I RGATCY 1 cut(s) 240
BstYI RGATCY 1 cut(s) 240
BstZI CGGCCG 1 cut(s) 392
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 6 cut(s) 153, 390, 394, 884, 1036, 1237
BtgI CCRYGG 1 cut(s) 1583
BtgZI GCGATG 2 cut(s) 503, 1050
BtrI CACGTC 1 cut(s) 868
BtsCI GGATG 1 cut(s) 1016
BtuMI TCGCGA 1 cut(s) 1268
Cfr10I RCCGGY 2 cut(s) 390, 1204
Cfr13I GGNCC 8 cut(s) 61, 151, 388, 883, 958, 1035, 1235, 1579
CseI GACGC 3 cut(s) 142, 1189, 1298
Csp6I GTAC 4 cut(s) 94, 895, 1165, 1174
CviQI GTAC 4 cut(s) 94, 895, 1165, 1174
DinI GGCGCC 2 cut(s) 859, 1122
DpnI GATC 6 cut(s) 120, 242, 740, 1265, 1284, 1395
DpnII GATC 6 cut(s) 118, 240, 738, 1263, 1282, 1393
DraI TTTAAA 1 cut(s) 640
EaeI YGGCCR 1 cut(s) 392
EagI CGGCCG 1 cut(s) 392
Eam1104I CTCTTC 2 cut(s) 764, 1475
EarI CTCTTC 2 cut(s) 764, 1475
EciI GGCGGA 3 cut(s) 214, 1473, 1587
Ecl136II GAGCTC 3 cut(s) 846, 924, 1299
EclXI CGGCCG 1 cut(s) 392
Eco24I GRGCYC 6 cut(s) 671, 848, 926, 1301, 1470, 1592
Eco32I GATATC 1 cut(s) 75
Eco47I GGWCC 3 cut(s) 61, 958, 1579
Eco47III AGCGCT 1 cut(s) 1277
Eco52I CGGCCG 1 cut(s) 392
Eco53kI GAGCTC 3 cut(s) 846, 924, 1299
Eco88I CYCGRG 3 cut(s) 385, 1358, 1408
Eco91I GGTNACC 1 cut(s) 299
EcoICRI GAGCTC 3 cut(s) 846, 924, 1299
EcoNI CCTNNNNNAGG 1 cut(s) 345
EcoO109I RGGNCCY 3 cut(s) 61, 958, 1579
EcoO65I GGTNACC 1 cut(s) 299
EcoRII CCWGG 5 cut(s) 33, 57, 291, 369, 1080
EcoRV GATATC 1 cut(s) 75
EcoT38I GRGCYC 6 cut(s) 671, 848, 926, 1301, 1470, 1592
EgeI GGCGCC 2 cut(s) 859, 1122
EheI GGCGCC 2 cut(s) 859, 1122
FalI AAGNNNNNCTT 2 cut(s) 1262, 1294
FaqI GGGAC 1 cut(s) 1565
FauI CCCGC 6 cut(s) 129, 658, 1003, 1057, 1457, 1511
FokI GGATG 1 cut(s) 1003
FriOI GRGCYC 6 cut(s) 671, 848, 926, 1301, 1470, 1592
FseI GGCCGGCC 1 cut(s) 394
FspAI RTGCGCAY 1 cut(s) 89
FspBI CTAG 1 cut(s) 347
FspI TGCGCA 1 cut(s) 89
GsaI CCCAGC 2 cut(s) 1014, 1235
GsuI CTGGAG 2 cut(s) 353, 583
HaeII RGCGCY 7 cut(s) 425, 813, 861, 939, 1124, 1279, 1435
HaeIII GGCC 6 cut(s) 153, 390, 394, 884, 1036, 1237
HapII CCGG 9 cut(s) 190, 317, 391, 572, 1025, 1107, 1205, 1546, 1599
HgaI GACGC 3 cut(s) 142, 1189, 1298
Hin1I GRCGYC 4 cut(s) 267, 858, 1121, 1181
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HinfI GANTC 5 cut(s) 403, 596, 1223, 1497, 1529
HpaII CCGG 9 cut(s) 190, 317, 391, 572, 1025, 1107, 1205, 1546, 1599
HphI GGTGA 1 cut(s) 1152
Hpy166II GTNNAC 6 cut(s) 112, 142, 196, 679, 871, 992
Hpy188III TCNNGA 4 cut(s) 766, 1267, 1286, 1408
Hpy8I GTNNAC 6 cut(s) 112, 142, 196, 679, 871, 992
Hpy99I CGWCG 9 cut(s) 136, 485, 559, 562, 1183, 1243, 1249, 1430, 1457
HpyAV CCTTC 2 cut(s) 1264, 1428
HpyCH4III ACNGT 4 cut(s) 283, 989, 1097, 1164
HpyCH4IV ACGT 5 cut(s) 45, 92, 267, 560, 867
HpyCH4V TGCA 6 cut(s) 104, 112, 542, 955, 1156, 1348
HpySE526I ACGT 5 cut(s) 45, 92, 267, 560, 867
Hsp92I GRCGYC 4 cut(s) 267, 858, 1121, 1181
KasI GGCGCC 2 cut(s) 857, 1120
KroI GCCGGC 1 cut(s) 390
KroNI GCCGGC 1 cut(s) 392
Kzo9I GATC 6 cut(s) 118, 240, 738, 1263, 1282, 1393
LguI GCTCTTC 2 cut(s) 764, 1475
Lsp1109I GCAGC 3 cut(s) 597, 1140, 1365
LweI GCATC 1 cut(s) 1025
MaeI CTAG 1 cut(s) 347
MaeII ACGT 5 cut(s) 45, 92, 267, 560, 867
MaeIII GTNAC 4 cut(s) 299, 983, 1055, 1158
MalI GATC 6 cut(s) 120, 242, 740, 1265, 1284, 1395
MbiI CCGCTC 2 cut(s) 1363, 1441
MboI GATC 6 cut(s) 118, 240, 738, 1263, 1282, 1393
MboII GAAGA 8 cut(s) 521, 781, 1040, 1053, 1340, 1462, 1561, 1564
MflI RGATCY 1 cut(s) 240
MhlI GDGCHC 9 cut(s) 114, 281, 671, 848, 926, 1301, 1470, 1508, 1592
MluCI AATT 4 cut(s) 635, 759, 1073, 1090
Mly113I GGCGCC 2 cut(s) 858, 1121
MlyI GAGTC 2 cut(s) 590, 1232
MmeI TCCRAC 6 cut(s) 52, 188, 463, 597, 1206, 1227
MroNI GCCGGC 1 cut(s) 390
MseI TTAA 1 cut(s) 639
MslI CAYNNNNRTG 2 cut(s) 416, 1205
MspI CCGG 9 cut(s) 190, 317, 391, 572, 1025, 1107, 1205, 1546, 1599
MspR9I CCNGG 9 cut(s) 35, 59, 191, 293, 317, 371, 1082, 1107, 1546
MvaI CCWGG 5 cut(s) 35, 59, 293, 371, 1082
MvnI CGCG 7 cut(s) 136, 1126, 1178, 1268, 1292, 1316, 1398
NaeI GCCGGC 1 cut(s) 392
NarI GGCGCC 2 cut(s) 858, 1121
NciI CCSGG 4 cut(s) 191, 317, 1107, 1546
NdeII GATC 6 cut(s) 118, 240, 738, 1263, 1282, 1393
NgoMIV GCCGGC 1 cut(s) 390
NlaIV GGNNCC 8 cut(s) 859, 885, 960, 1122, 1209, 1236, 1581, 1589
NmeAIII GCCGAG 4 cut(s) 834, 867, 945, 1209
NmuCI GTSAC 2 cut(s) 1055, 1158
NruI TCGCGA 1 cut(s) 1268
NsbI TGCGCA 1 cut(s) 89
NspI RCATGY 3 cut(s) 355, 451, 1618
NspV TTCGAA 1 cut(s) 566
PaeI GCATGC 2 cut(s) 355, 451
PaeR7I CTCGAG 2 cut(s) 1358, 1408
PciI ACATGT 1 cut(s) 1614
PciSI GCTCTTC 2 cut(s) 764, 1475
PcsI WCGNNNNNNNCGW 2 cut(s) 563, 1434
PdiI GCCGGC 1 cut(s) 392
PfeI GAWTC 3 cut(s) 403, 1497, 1529
Pfl23II CGTACG 2 cut(s) 93, 1164
PflMI CCANNNNNTGG 2 cut(s) 34, 105
PfoI TCCNGGA 1 cut(s) 33
Ple19I CGATCG 1 cut(s) 1266
PleI GAGTC 2 cut(s) 590, 1231
PluTI GGCGCC 2 cut(s) 861, 1124
PpsI GAGTC 2 cut(s) 590, 1231
Ppu21I YACGTR 1 cut(s) 93
PpuMI RGGWCCY 3 cut(s) 61, 958, 1579
PscI ACATGT 1 cut(s) 1614
PshAI GACNNNNGTC 1 cut(s) 1114
Psp124BI GAGCTC 3 cut(s) 848, 926, 1301
Psp5II RGGWCCY 3 cut(s) 61, 958, 1579
Psp6I CCWGG 5 cut(s) 33, 57, 291, 369, 1080
PspEI GGTNACC 1 cut(s) 299
PspFI CCCAGC 2 cut(s) 1010, 1231
PspGI CCWGG 5 cut(s) 33, 57, 291, 369, 1080
PspLI CGTACG 2 cut(s) 93, 1164
PspN4I GGNNCC 8 cut(s) 859, 885, 960, 1122, 1209, 1236, 1581, 1589
PspPI GGNCC 8 cut(s) 61, 151, 388, 883, 958, 1035, 1235, 1579
PspPPI RGGWCCY 3 cut(s) 61, 958, 1579
PspXI VCTCGAGB 1 cut(s) 1358
PstI CTGCAG 1 cut(s) 1158
PsuI RGATCY 1 cut(s) 240
PvuI CGATCG 1 cut(s) 1266
RgaI GCGATCGC 1 cut(s) 1266
RigI GGCCGGCC 1 cut(s) 394
RruI TCGCGA 1 cut(s) 1268
RsaI GTAC 4 cut(s) 95, 896, 1166, 1175
RsaNI GTAC 4 cut(s) 94, 895, 1165, 1174
RseI CAYNNNNRTG 2 cut(s) 416, 1205
SacI GAGCTC 3 cut(s) 848, 926, 1301
SapI GCTCTTC 2 cut(s) 764, 1475
SaqAI TTAA 1 cut(s) 639
Sau3AI GATC 6 cut(s) 118, 240, 738, 1263, 1282, 1393
Sau96I GGNCC 8 cut(s) 61, 151, 388, 883, 958, 1035, 1235, 1579
SchI GAGTC 2 cut(s) 590, 1232
ScrFI CCNGG 9 cut(s) 35, 59, 191, 293, 317, 371, 1082, 1107, 1546
SduI GDGCHC 9 cut(s) 114, 281, 671, 848, 926, 1301, 1470, 1508, 1592
SfaAI GCGATCGC 1 cut(s) 1266
SfaNI GCATC 1 cut(s) 1025
SfcI CTRYAG 1 cut(s) 1154
SfoI GGCGCC 2 cut(s) 859, 1122
Sfr274I CTCGAG 2 cut(s) 1358, 1408
SfuI TTCGAA 1 cut(s) 566
SgfI GCGATCGC 1 cut(s) 1266
SinI GGWCC 3 cut(s) 61, 958, 1579
SlaI CTCGAG 2 cut(s) 1358, 1408
SmiMI CAYNNNNRTG 2 cut(s) 416, 1205
SmlI CTYRAG 2 cut(s) 1358, 1408
SmoI CTYRAG 2 cut(s) 1358, 1408
SphI GCATGC 2 cut(s) 355, 451
Sse9I AATT 4 cut(s) 635, 759, 1073, 1090
SspDI GGCGCC 2 cut(s) 857, 1120
SspI AATATT 1 cut(s) 1489
SspMI CTAG 1 cut(s) 347
SstI GAGCTC 3 cut(s) 848, 926, 1301
StyD4I CCNGG 9 cut(s) 33, 57, 189, 291, 315, 369, 1080, 1105, 1544
TaaI ACNGT 4 cut(s) 283, 989, 1097, 1164
TaiI ACGT 5 cut(s) 48, 95, 270, 563, 870
TaqI TCGA 8 cut(s) 121, 131, 566, 737, 1193, 1359, 1409, 1500
TasI AATT 4 cut(s) 635, 759, 1073, 1090
TauI GCSGC 8 cut(s) 381, 397, 1126, 1259, 1262, 1384, 1444, 1447
TfiI GAWTC 3 cut(s) 403, 1497, 1529
Tru1I TTAA 1 cut(s) 639
Tru9I TTAA 1 cut(s) 639
TseFI GTSAC 2 cut(s) 1055, 1158
TseI GCWGC 3 cut(s) 585, 1153, 1378
Tsp45I GTSAC 2 cut(s) 1055, 1158
TspDTI ATGAA 1 cut(s) 1204
TspGWI ACGGA 7 cut(s) 12, 912, 916, 1126, 1235, 1388, 1600
Van91I CCANNNNNTGG 2 cut(s) 34, 105
VneI GTGCAC 1 cut(s) 110
VpaK11BI GGWCC 3 cut(s) 61, 958, 1579
XagI CCTNNNNNAGG 1 cut(s) 345
XapI RAATTY 2 cut(s) 635, 1073
XceI RCATGY 3 cut(s) 355, 451, 1618
XhoI CTCGAG 2 cut(s) 1358, 1408
XspI CTAG 1 cut(s) 347
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.