FvH4_4g05410

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011936
Physical Location & Seq
Reverse (-)
4676346 .. 4679881
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05410.t1

Sequence Viewer

Length: 432 bp
ATGACGGAGGCGGTGATCAGAAACAAGCCAGGAATGGCGAGCGTGAAGGACATGCCGGTTCTCCAAGACGGTCCGCCGCCGGGTGGGTTCCCTCCTGCCCGATACGCTCGTCGGATCCCCAATAAGGGTCCCAGCGCCGTCGCCATCTTCCTCGCTGCCTTCGGTACCTTCTCCTGGGGCATGTACCAGGTCGGCAAGGGCAACAAGATCCGAAGGGCGCTGAAGGAAGAAAAGTATGCTGCTCGCAGAGCAATACTTCCAGTGCTTCAAGCTGAAGAAGACGAAAGATTCGTCAAAGAGTGGAAAAAGTATCTTGATTATGAAGCTGAGGTTATGAAGGATGTGCCTAACTGGAAAGTTGGTGAGAGTGTCTACAACTCTGGCAGATGGATGCCCCCAGCCACTGGCGAGCTGCGCCCTGAAGTCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
GRIM-19

Protein Analysis

144

Amino Acids

16.18

Weight (kDa)

9.58

Isoelectric Point (pI)

69.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRIM-19 PF06212 20 - 138 4.4e-40 GRIM-19 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011936)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04630 AT2G33220 AT2G33220
fragaria_vesca FvH4_4g05410
malus_domestica MD13G1228700.v1.1 MD16G1234000.v1.1
prunus_persica Prupe.1G056200_v2.0.a1
pyrus_communis pycom13g20200 pycom16g19500
rosa_chinensis RchiOBHm_Chr4g0396561
rosa_laevigata RLG00000009524
rosa_multiflora Rmu_sc0001781.1_g000004
rosa_roxburghii Rroxscaffold_5G00341410
rosa_rugosa Rorug03G0349200
rosa_samantha Rh4AG069900 Rh4BG067500 Rh4CG075400 Rh4DG065300
rosa_wichuraiana Rw4G005600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 1 cut(s) 404
AccI GTMKAC 1 cut(s) 372
AciI CCGC 3 cut(s) 11, 74, 77
AclWI GGATC 3 cut(s) 109, 122, 202
AcuI CTGAAG 2 cut(s) 242, 294
AfaI GTAC 2 cut(s) 166, 185
AfiI CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
AgsI TTSAA 1 cut(s) 269
AjnI CCWGG 3 cut(s) 28, 173, 186
AluBI AGCT 3 cut(s) 272, 326, 412
AluI AGCT 3 cut(s) 272, 326, 412
AlwI GGATC 3 cut(s) 109, 122, 202
AlwNI CAGNNNCTG 1 cut(s) 404
ApeKI GCWGC 3 cut(s) 155, 239, 412
ArsI GACNNNNNNTTYG 4 cut(s) 272, 276, 304, 308
Asp718I GGTACC 1 cut(s) 164
AspLEI GCGC 3 cut(s) 137, 220, 417
AspS9I GGNCC 2 cut(s) 71, 128
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 25, 374
AvaII GGWCC 2 cut(s) 71, 128
BaeI ACNNNNGTAYC 2 cut(s) 94, 127
BamHI GGATCC 1 cut(s) 114
BanI GGYRCC 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 285
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 3 cut(s) 142, 226, 399
BccI CCATC 2 cut(s) 152, 381
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 3 cut(s) 30, 175, 188
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 1 cut(s) 81
BfoI RGCGCY 2 cut(s) 138, 221
BisI GCNGC 4 cut(s) 77, 156, 240, 413
BlsI GCNGC 4 cut(s) 78, 157, 241, 414
Bme1390I CCNGG 4 cut(s) 30, 81, 175, 188
Bme18I GGWCC 2 cut(s) 71, 128
BmgT120I GGNCC 2 cut(s) 71, 128
BmiI GGNNCC 5 cut(s) 89, 116, 129, 130, 166
BmrFI CCNGG 4 cut(s) 30, 81, 175, 188
BmsI GCATC 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 174
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 260, 356, 409
BseBI CCWGG 3 cut(s) 30, 175, 188
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 2 cut(s) 346, 396
BseLI CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 3 cut(s) 260, 356, 409
BseXI GCAGC 3 cut(s) 142, 226, 399
BseYI CCCAGC 2 cut(s) 131, 397
BshNI GGYRCC 1 cut(s) 164
BsiSI CCGG 2 cut(s) 56, 80
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
BsmFI GGGAC 1 cut(s) 114
Bsp143I GATC 3 cut(s) 15, 114, 207
BspACI CCGC 3 cut(s) 11, 74, 77
BspCNI CTCAG 1 cut(s) 319
BspLI GGNNCC 5 cut(s) 89, 116, 129, 130, 166
BspPI GGATC 3 cut(s) 109, 122, 202
BspT107I GGYRCC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 3 cut(s) 260, 356, 409
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 3 cut(s) 15, 114, 207
Bst2UI CCWGG 3 cut(s) 30, 175, 188
Bst4CI ACNGT 1 cut(s) 71
BstC8I GCNNGC 3 cut(s) 40, 244, 410
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 2 cut(s) 346, 396
BstH2I RGCGCY 2 cut(s) 138, 221
BstHHI GCGC 3 cut(s) 137, 220, 417
BstKTI GATC 3 cut(s) 18, 117, 210
BstMBI GATC 3 cut(s) 15, 114, 207
BstMWI GCNNNNNNNGC 3 cut(s) 104, 248, 414
BstNI CCWGG 3 cut(s) 30, 175, 188
BstNSI RCATGY 2 cut(s) 55, 184
BstSCI CCNGG 4 cut(s) 28, 79, 173, 186
BstV1I GCAGC 3 cut(s) 142, 226, 399
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 2 cut(s) 114, 207
BstYI RGATCY 2 cut(s) 114, 207
BtsCI GGATG 2 cut(s) 346, 396
BtsIMutI CAGTG 2 cut(s) 267, 402
Cac8I GCNNGC 3 cut(s) 40, 244, 410
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 3 cut(s) 137, 220, 417
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 71, 128
CpoI CGGWCCG 1 cut(s) 71
CsiI ACCWGGT 1 cut(s) 186
Csp6I GTAC 2 cut(s) 165, 184
CspI CGGWCCG 1 cut(s) 71
CviAII CATG 2 cut(s) 52, 181
CviJI RGCY 5 cut(s) 28, 272, 326, 401, 412
CviKI_1 RGCY 5 cut(s) 28, 272, 326, 401, 412
CviQI GTAC 2 cut(s) 165, 184
DdeI CTNAG 1 cut(s) 327
DpnI GATC 3 cut(s) 17, 116, 209
DpnII GATC 3 cut(s) 15, 114, 207
EciI GGCGGA 1 cut(s) 63
Eco47I GGWCC 2 cut(s) 71, 128
Eco57I CTGAAG 2 cut(s) 242, 294
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 28, 173, 186
FaeI CATG 2 cut(s) 55, 184
FaiI YATR 5 cut(s) 53, 182, 237, 321, 335
FaqI GGGAC 1 cut(s) 114
FatI CATG 2 cut(s) 51, 180
FbaI TGATCA 1 cut(s) 15
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 4 cut(s) 77, 156, 240, 413
FokI GGATG 2 cut(s) 353, 403
Fsp4HI GCNGC 4 cut(s) 77, 156, 240, 413
GlaI GCGC 3 cut(s) 136, 219, 416
GluI GCNGC 4 cut(s) 77, 156, 240, 413
GsaI CCCAGC 2 cut(s) 135, 401
HaeII RGCGCY 2 cut(s) 138, 221
HapII CCGG 2 cut(s) 56, 80
HhaI GCGC 3 cut(s) 137, 220, 417
Hin1II CATG 2 cut(s) 55, 184
Hin6I GCGC 3 cut(s) 135, 218, 415
HinP1I GCGC 3 cut(s) 135, 218, 415
HinfI GANTC 1 cut(s) 288
HpaII CCGG 2 cut(s) 56, 80
HphI GGTGA 2 cut(s) 25, 374
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 3 cut(s) 20, 114, 212
Hpy188III TCNNGA 1 cut(s) 314
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 2 cut(s) 114, 143
HpyAV CCTTC 6 cut(s) 40, 169, 178, 207, 217, 331
HpyCH4III ACNGT 1 cut(s) 71
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 248, 414
HpyF3I CTNAG 1 cut(s) 327
Hsp92II CATG 2 cut(s) 55, 184
HspAI GCGC 3 cut(s) 135, 218, 415
KflI GGGWCCC 1 cut(s) 128
KpnI GGTACC 1 cut(s) 168
Ksp22I TGATCA 1 cut(s) 15
Kzo9I GATC 3 cut(s) 15, 114, 207
Lsp1109I GCAGC 3 cut(s) 142, 226, 399
LweI GCATC 1 cut(s) 381
MabI ACCWGGT 1 cut(s) 186
MalI GATC 3 cut(s) 17, 116, 209
MboI GATC 3 cut(s) 15, 114, 207
MboII GAAGA 4 cut(s) 139, 239, 287, 290
MflI RGATCY 2 cut(s) 114, 207
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 3 cut(s) 102, 161, 322
MspI CCGG 2 cut(s) 56, 80
MspR9I CCNGG 4 cut(s) 30, 81, 175, 188
MvaI CCWGG 3 cut(s) 30, 175, 188
MwoI GCNNNNNNNGC 3 cut(s) 104, 248, 414
NciI CCSGG 1 cut(s) 81
NdeII GATC 3 cut(s) 15, 114, 207
NlaIII CATG 2 cut(s) 55, 184
NlaIV GGNNCC 5 cut(s) 89, 116, 129, 130, 166
NspI RCATGY 2 cut(s) 55, 184
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 1 cut(s) 288
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 4 cut(s) 78, 157, 241, 414
PpuMI RGGWCCY 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 3 cut(s) 28, 173, 186
PspFI CCCAGC 2 cut(s) 131, 397
PspGI CCWGG 3 cut(s) 28, 173, 186
PspN4I GGNNCC 5 cut(s) 89, 116, 129, 130, 166
PspPI GGNCC 2 cut(s) 71, 128
PspPPI RGGWCCY 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 2 cut(s) 114, 207
RsaI GTAC 2 cut(s) 166, 185
RsaNI GTAC 2 cut(s) 165, 184
Rsr2I CGGWCCG 1 cut(s) 71
RsrII CGGWCCG 1 cut(s) 71
SatI GCNGC 4 cut(s) 77, 156, 240, 413
Sau3AI GATC 3 cut(s) 15, 114, 207
Sau96I GGNCC 2 cut(s) 71, 128
ScrFI CCNGG 4 cut(s) 30, 81, 175, 188
SetI ASST 6 cut(s) 170, 192, 274, 328, 333, 414
SexAI ACCWGGT 1 cut(s) 186
SfaNI GCATC 1 cut(s) 381
SinI GGWCC 2 cut(s) 71, 128
SsiI CCGC 3 cut(s) 11, 74, 77
StyD4I CCNGG 4 cut(s) 28, 79, 173, 186
TaaI ACNGT 1 cut(s) 71
TauI GCSGC 1 cut(s) 79
TfiI GAWTC 1 cut(s) 288
TscAI CASTG 2 cut(s) 267, 409
TseI GCWGC 3 cut(s) 155, 239, 412
TspDTI ATGAA 2 cut(s) 336, 350
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 2 cut(s) 267, 409
Van91I CCANNNNNTGG 1 cut(s) 404
VpaK11BI GGWCC 2 cut(s) 71, 128
XceI RCATGY 2 cut(s) 55, 184
XmiI GTMKAC 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.