MD13G1228700.v1.1

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011936
Physical Location & Seq
Forward (+)
22439724 .. 22442963
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1228700.v1.1.491

Sequence Viewer

Length: 432 bp
ATGACAGAGGCAGTGATAAGAAAAAAGCCGGGGATGGCGAGCGTGAAGGACATGCCGGTTCTCCAGGACGGTCCGCCGCCGGGGGGTTTTGCTCCGGTTCGATACGCTCGCCGGATCCCCAACAAAGGTCCCAGCTCAGTTGCCATATTCCTTGCTGCCTTTGGTACCTTCTCTTGGGGCATGTACCAAGTCGGAAAGGGCAACAAGATCCGAAGGGCGCTCAAGGAAGAAAAGTATGCTGCTCGCAGAGCAATACTGCCAGTGCTTCAAGCTGAAGAAGATGAAAGATATGTCAAAGAGTGGAAAAAGTATCTTGAGTACGAGGCTGAAGTAATGAAGGATGTGCCAAATTGGAAAGTTGGCGAGAGTGTCTACAATTCTGGAAAATGGATGCCCCCAGCAACGGGCGAGCTCCGCCCTGAAGTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
GRIM-19

Protein Analysis

144

Amino Acids

16.22

Weight (kDa)

9.6

Isoelectric Point (pI)

61.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRIM-19 PF06212 20 - 138 6.3e-41 GRIM-19 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011936)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04630 AT2G33220 AT2G33220
fragaria_vesca FvH4_4g05410
malus_domestica MD13G1228700.v1.1 MD16G1234000.v1.1
prunus_persica Prupe.1G056200_v2.0.a1
pyrus_communis pycom13g20200 pycom16g19500
rosa_chinensis RchiOBHm_Chr4g0396561
rosa_laevigata RLG00000009524
rosa_multiflora Rmu_sc0001781.1_g000004
rosa_roxburghii Rroxscaffold_5G00341410
rosa_rugosa Rorug03G0349200
rosa_samantha Rh4AG069900 Rh4BG067500 Rh4CG075400 Rh4DG065300
rosa_wichuraiana Rw4G005600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 372
AciI CCGC 3 cut(s) 74, 77, 415
AclWI GGATC 3 cut(s) 109, 122, 202
AcuI CTGAAG 2 cut(s) 294, 348
AfaI GTAC 3 cut(s) 166, 185, 320
AfiI CCNNNNNNNGG 6 cut(s) 80, 83, 125, 174, 403, 404
AgsI TTSAA 1 cut(s) 269
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 3 cut(s) 135, 272, 412
AluI AGCT 3 cut(s) 135, 272, 412
Alw21I GWGCWC 1 cut(s) 414
AlwI GGATC 3 cut(s) 109, 122, 202
ApeKI GCWGC 2 cut(s) 155, 239
Asp718I GGTACC 1 cut(s) 164
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 2 cut(s) 71, 128
AsuC2I CCSGG 2 cut(s) 30, 81
AvaII GGWCC 2 cut(s) 71, 128
BamHI GGATCC 1 cut(s) 114
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 1 cut(s) 414
BbvI GCAGC 2 cut(s) 142, 226
BccI CCATC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 65
BcnI CCSGG 2 cut(s) 30, 81
BfoI RGCGCY 1 cut(s) 221
BisI GCNGC 3 cut(s) 77, 156, 240
BlsI GCNGC 3 cut(s) 78, 157, 241
Bme1390I CCNGG 3 cut(s) 30, 65, 81
Bme18I GGWCC 2 cut(s) 71, 128
BmgT120I GGNCC 2 cut(s) 71, 128
BmiI GGNNCC 3 cut(s) 116, 130, 166
BmrFI CCNGG 3 cut(s) 30, 65, 81
BmsI GCATC 1 cut(s) 381
BpmI CTGGAG 1 cut(s) 47
BpuEI CTTGAG 2 cut(s) 206, 335
BpuMI CCSGG 2 cut(s) 30, 81
BsaJI CCNNGG 2 cut(s) 29, 80
BsaWI WCCGGW 1 cut(s) 94
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 83, 125, 174, 403, 404
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 260
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 29, 80
BseGI GGATG 3 cut(s) 39, 346, 396
BseLI CCNNNNNNNGG 6 cut(s) 80, 83, 125, 174, 403, 404
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 260
BseXI GCAGC 2 cut(s) 142, 226
BseYI CCCAGC 2 cut(s) 131, 397
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 5 cut(s) 29, 56, 80, 95, 112
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 6 cut(s) 80, 83, 125, 174, 403, 404
BsmFI GGGAC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 414
Bsp143I GATC 2 cut(s) 114, 207
BspACI CCGC 3 cut(s) 74, 77, 415
BspCNI CTCAG 1 cut(s) 149
BspLI GGNNCC 3 cut(s) 116, 130, 166
BspPI GGATC 3 cut(s) 109, 122, 202
BspT107I GGYRCC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 29, 80
BssMI GATC 2 cut(s) 114, 207
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 40, 109, 244, 410
BstDEI CTNAG 1 cut(s) 136
BstF5I GGATG 3 cut(s) 39, 346, 396
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 2 cut(s) 117, 210
BstMBI GATC 2 cut(s) 114, 207
BstMWI GCNNNNNNNGC 2 cut(s) 248, 414
BstNI CCWGG 1 cut(s) 65
BstNSI RCATGY 2 cut(s) 55, 184
BstSCI CCNGG 3 cut(s) 28, 63, 79
BstV1I GCAGC 2 cut(s) 142, 226
BstX2I RGATCY 2 cut(s) 114, 207
BstYI RGATCY 2 cut(s) 114, 207
BtsCI GGATG 3 cut(s) 39, 346, 396
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 2 cut(s) 18, 267
Cac8I GCNNGC 4 cut(s) 40, 109, 244, 410
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 71, 128
CpoI CGGWCCG 1 cut(s) 71
Csp6I GTAC 3 cut(s) 165, 184, 319
CspI CGGWCCG 1 cut(s) 71
CviAII CATG 2 cut(s) 52, 181
CviJI RGCY 5 cut(s) 28, 135, 272, 326, 412
CviKI_1 RGCY 5 cut(s) 28, 135, 272, 326, 412
CviQI GTAC 3 cut(s) 165, 184, 319
DdeI CTNAG 1 cut(s) 136
DpnI GATC 2 cut(s) 116, 209
DpnII GATC 2 cut(s) 114, 207
EciI GGCGGA 2 cut(s) 63, 404
Ecl136II GAGCTC 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 2 cut(s) 71, 128
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 2 cut(s) 294, 348
EcoICRI GAGCTC 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 63
EcoT38I GRGCYC 1 cut(s) 414
FaeI CATG 2 cut(s) 55, 184
FaiI YATR 5 cut(s) 53, 146, 182, 237, 291
FaqI GGGAC 1 cut(s) 114
FatI CATG 2 cut(s) 51, 180
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 77, 156, 240
FokI GGATG 3 cut(s) 46, 353, 403
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 3 cut(s) 77, 156, 240
GlaI GCGC 1 cut(s) 219
GluI GCNGC 3 cut(s) 77, 156, 240
GsaI CCCAGC 2 cut(s) 135, 401
GsuI CTGGAG 1 cut(s) 47
HaeII RGCGCY 1 cut(s) 221
HapII CCGG 5 cut(s) 29, 56, 80, 95, 112
HhaI GCGC 1 cut(s) 220
Hin1II CATG 2 cut(s) 55, 184
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HpaII CCGG 5 cut(s) 29, 56, 80, 95, 112
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 194, 212
Hpy188III TCNNGA 2 cut(s) 314, 381
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 4 cut(s) 40, 178, 207, 331
HpyCH4III ACNGT 1 cut(s) 71
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 414
HpyF3I CTNAG 1 cut(s) 136
Hsp92II CATG 2 cut(s) 55, 184
HspAI GCGC 1 cut(s) 218
KpnI GGTACC 1 cut(s) 168
Kzo9I GATC 2 cut(s) 114, 207
LmnI GCTCC 2 cut(s) 97, 417
Lsp1109I GCAGC 2 cut(s) 142, 226
LweI GCATC 1 cut(s) 381
MalI GATC 2 cut(s) 116, 209
MboI GATC 2 cut(s) 114, 207
MboII GAAGA 3 cut(s) 239, 287, 290
MflI RGATCY 2 cut(s) 114, 207
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 2 cut(s) 349, 376
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 1 cut(s) 316
MspI CCGG 5 cut(s) 29, 56, 80, 95, 112
MspR9I CCNGG 3 cut(s) 30, 65, 81
MvaI CCWGG 1 cut(s) 65
MwoI GCNNNNNNNGC 2 cut(s) 248, 414
NciI CCSGG 2 cut(s) 30, 81
NdeII GATC 2 cut(s) 114, 207
NlaIII CATG 2 cut(s) 55, 184
NlaIV GGNNCC 3 cut(s) 116, 130, 166
NspI RCATGY 2 cut(s) 55, 184
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 3 cut(s) 78, 157, 241
PpuMI RGGWCCY 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 63
PspFI CCCAGC 2 cut(s) 131, 397
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 3 cut(s) 116, 130, 166
PspPI GGNCC 2 cut(s) 71, 128
PspPPI RGGWCCY 1 cut(s) 128
PsuI RGATCY 2 cut(s) 114, 207
RsaI GTAC 3 cut(s) 166, 185, 320
RsaNI GTAC 3 cut(s) 165, 184, 319
Rsr2I CGGWCCG 1 cut(s) 71
RsrII CGGWCCG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 414
SatI GCNGC 3 cut(s) 77, 156, 240
Sau3AI GATC 2 cut(s) 114, 207
Sau96I GGNCC 2 cut(s) 71, 128
ScrFI CCNGG 3 cut(s) 30, 65, 81
SduI GDGCHC 1 cut(s) 414
SetI ASST 5 cut(s) 130, 137, 170, 274, 414
SfaNI GCATC 1 cut(s) 381
SinI GGWCC 2 cut(s) 71, 128
SmlI CTYRAG 2 cut(s) 221, 314
SmoI CTYRAG 2 cut(s) 221, 314
Sse9I AATT 2 cut(s) 349, 376
SsiI CCGC 3 cut(s) 74, 77, 415
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 28, 63, 79
TaaI ACNGT 1 cut(s) 71
TaqI TCGA 1 cut(s) 100
TasI AATT 2 cut(s) 349, 376
TauI GCSGC 1 cut(s) 79
TscAI CASTG 2 cut(s) 18, 267
TseI GCWGC 2 cut(s) 155, 239
TspDTI ATGAA 2 cut(s) 297, 350
TspRI CASTG 2 cut(s) 18, 267
VpaK11BI GGWCC 2 cut(s) 71, 128
XceI RCATGY 2 cut(s) 55, 184
XmiI GTMKAC 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.