pycom16g19500

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011936
Physical Location & Seq
Reverse (-)
16615754 .. 16618468
2715 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g19500.1

Sequence Viewer

Length: 432 bp
ATGACAGAGGCAGTGATAAGAAATAAACCGGGTATGGCGAGCGTGAAGGACATGCCGGTTCTGCAGGACGGTCCGCCGCCGGGTGGATTCGCTCCGGTCCGGTACGCTCGCCGGATCCCCAACAAGGGTCCCAGCTCAGTGGCCATATTCCTCGCCGCCTTCGGTACCTTCTCTTGGGGCATGTACCAAGTCGGAAAGGGCAACAAGATCCGCAGGGCGCTCAAGGAAGAAAAGTATGCTGCTCGCAGAGCAATACTGCCGGTGCTTCAAGCTGAAGAAGATGAAAGATATGTCAAAGAGTGGAAGAAGTATCTCGACTATGAGGCTGAAGTCATGAAGGACGTGCCTAATTGGAAAGTTGGTGAGAGTGTCTACAACTCTGGAAAATGGATGCCCCCAGCAACAGGCGAGCTCCGCCCTGAAGTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
GRIM-19

Protein Analysis

144

Amino Acids

16.19

Weight (kDa)

9.52

Isoelectric Point (pI)

64.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRIM-19 PF06212 20 - 138 5e-41 GRIM-19 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011936)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04630 AT2G33220 AT2G33220
fragaria_vesca FvH4_4g05410
malus_domestica MD13G1228700.v1.1 MD16G1234000.v1.1
prunus_persica Prupe.1G056200_v2.0.a1
pyrus_communis pycom13g20200 pycom16g19500
rosa_chinensis RchiOBHm_Chr4g0396561
rosa_laevigata RLG00000009524
rosa_multiflora Rmu_sc0001781.1_g000004
rosa_roxburghii Rroxscaffold_5G00341410
rosa_rugosa Rorug03G0349200
rosa_samantha Rh4AG069900 Rh4BG067500 Rh4CG075400 Rh4DG065300
rosa_wichuraiana Rw4G005600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 372
AciI CCGC 5 cut(s) 74, 77, 156, 211, 415
AclWI GGATC 3 cut(s) 109, 122, 202
AcoI YGGCCR 1 cut(s) 141
AcuI CTGAAG 2 cut(s) 294, 348
AfaI GTAC 3 cut(s) 104, 166, 185
AfiI CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
AgsI TTSAA 1 cut(s) 269
AjiI CACGTC 1 cut(s) 343
AluBI AGCT 3 cut(s) 135, 272, 412
AluI AGCT 3 cut(s) 135, 272, 412
Alw21I GWGCWC 1 cut(s) 414
AlwI GGATC 3 cut(s) 109, 122, 202
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 239
Asp718I GGTACC 1 cut(s) 164
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 3 cut(s) 71, 97, 128
AsuC2I CCSGG 2 cut(s) 30, 81
AsuHPI GGTGA 1 cut(s) 374
AvaII GGWCC 3 cut(s) 71, 97, 128
BalI TGGCCA 1 cut(s) 143
BamHI GGATCC 1 cut(s) 114
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 1 cut(s) 414
BbvI GCAGC 1 cut(s) 226
BcnI CCSGG 2 cut(s) 30, 81
BfmI CTRYAG 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 221
BisI GCNGC 3 cut(s) 77, 156, 240
BlsI GCNGC 3 cut(s) 78, 157, 241
Bme1390I CCNGG 2 cut(s) 30, 81
Bme18I GGWCC 3 cut(s) 71, 97, 128
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 3 cut(s) 71, 97, 128
BmiI GGNNCC 4 cut(s) 116, 129, 130, 166
BmrFI CCNGG 2 cut(s) 30, 81
BmsI GCATC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 206
BpuMI CCSGG 2 cut(s) 30, 81
BsaWI WCCGGW 2 cut(s) 94, 99
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
Bse118I RCCGGY 2 cut(s) 55, 259
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
BseMII CTCAG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 226
BseYI CCCAGC 2 cut(s) 131, 397
BshFI GGCC 1 cut(s) 143
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 7 cut(s) 29, 56, 80, 95, 100, 112, 260
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 414
Bsp143I GATC 2 cut(s) 114, 207
BspACI CCGC 5 cut(s) 74, 77, 156, 211, 415
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 1 cut(s) 149
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 4 cut(s) 116, 129, 130, 166
BspMAI CTGCAG 1 cut(s) 66
BspPI GGATC 3 cut(s) 109, 122, 202
BspT107I GGYRCC 1 cut(s) 164
BsrFI RCCGGY 2 cut(s) 55, 259
BssAI RCCGGY 2 cut(s) 55, 259
BssMI GATC 2 cut(s) 114, 207
Bst4CI ACNGT 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 40, 109, 244, 410
BstDEI CTNAG 1 cut(s) 136
BstF5I GGATG 1 cut(s) 396
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 2 cut(s) 117, 210
BstMBI GATC 2 cut(s) 114, 207
BstMWI GCNNNNNNNGC 3 cut(s) 61, 248, 414
BstNSI RCATGY 2 cut(s) 55, 184
BstSCI CCNGG 2 cut(s) 28, 79
BstSFI CTRYAG 1 cut(s) 62
BstV1I GCAGC 1 cut(s) 226
BstX2I RGATCY 2 cut(s) 114, 207
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 2 cut(s) 114, 207
BsuRI GGCC 1 cut(s) 143
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 396
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 2 cut(s) 18, 144
Cac8I GCNNGC 4 cut(s) 40, 109, 244, 410
CciI TCATGA 1 cut(s) 333
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 2 cut(s) 55, 259
Cfr13I GGNCC 3 cut(s) 71, 97, 128
CpoI CGGWCCG 2 cut(s) 71, 97
Csp6I GTAC 3 cut(s) 103, 165, 184
CspI CGGWCCG 2 cut(s) 71, 97
CviAII CATG 3 cut(s) 52, 181, 334
CviJI RGCY 5 cut(s) 135, 143, 272, 326, 412
CviKI_1 RGCY 5 cut(s) 135, 143, 272, 326, 412
CviQI GTAC 3 cut(s) 103, 165, 184
DdeI CTNAG 1 cut(s) 136
DpnI GATC 2 cut(s) 116, 209
DpnII GATC 2 cut(s) 114, 207
EaeI YGGCCR 1 cut(s) 141
EciI GGCGGA 2 cut(s) 63, 404
Ecl136II GAGCTC 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 3 cut(s) 71, 97, 128
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 2 cut(s) 294, 348
EcoICRI GAGCTC 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 128
EcoT38I GRGCYC 1 cut(s) 414
FaeI CATG 3 cut(s) 55, 184, 337
FaiI YATR 8 cut(s) 35, 53, 146, 182, 237, 291, 321, 335
FaqI GGGAC 1 cut(s) 114
FatI CATG 3 cut(s) 51, 180, 333
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 77, 156, 240
FokI GGATG 1 cut(s) 403
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 3 cut(s) 77, 156, 240
GlaI GCGC 1 cut(s) 219
GluI GCNGC 3 cut(s) 77, 156, 240
GsaI CCCAGC 2 cut(s) 135, 401
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 1 cut(s) 143
HapII CCGG 7 cut(s) 29, 56, 80, 95, 100, 112, 260
HhaI GCGC 1 cut(s) 220
Hin1II CATG 3 cut(s) 55, 184, 337
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HinfI GANTC 1 cut(s) 87
HpaII CCGG 7 cut(s) 29, 56, 80, 95, 100, 112, 260
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 3 cut(s) 314, 334, 381
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 4 cut(s) 40, 169, 178, 331
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 248, 414
HpyF3I CTNAG 1 cut(s) 136
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 3 cut(s) 55, 184, 337
HspAI GCGC 1 cut(s) 218
KflI GGGWCCC 1 cut(s) 128
KpnI GGTACC 1 cut(s) 168
Kzo9I GATC 2 cut(s) 114, 207
LmnI GCTCC 2 cut(s) 97, 417
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 1 cut(s) 381
MaeII ACGT 1 cut(s) 342
MalI GATC 2 cut(s) 116, 209
MboI GATC 2 cut(s) 114, 207
MboII GAAGA 4 cut(s) 239, 287, 290, 316
MflI RGATCY 2 cut(s) 114, 207
MhlI GDGCHC 1 cut(s) 414
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 1 cut(s) 349
MluNI TGGCCA 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 2 cut(s) 161, 316
Mox20I TGGCCA 1 cut(s) 143
MscI TGGCCA 1 cut(s) 143
Msp20I TGGCCA 1 cut(s) 143
MspI CCGG 7 cut(s) 29, 56, 80, 95, 100, 112, 260
MspR9I CCNGG 2 cut(s) 30, 81
MwoI GCNNNNNNNGC 3 cut(s) 61, 248, 414
NciI CCSGG 2 cut(s) 30, 81
NdeII GATC 2 cut(s) 114, 207
NlaIII CATG 3 cut(s) 55, 184, 337
NlaIV GGNNCC 4 cut(s) 116, 129, 130, 166
NspI RCATGY 2 cut(s) 55, 184
PagI TCATGA 1 cut(s) 333
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 3 cut(s) 78, 157, 241
PpuMI RGGWCCY 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 128
PspFI CCCAGC 2 cut(s) 131, 397
PspN4I GGNNCC 4 cut(s) 116, 129, 130, 166
PspPI GGNCC 3 cut(s) 71, 97, 128
PspPPI RGGWCCY 1 cut(s) 128
PstI CTGCAG 1 cut(s) 66
PsuI RGATCY 2 cut(s) 114, 207
RsaI GTAC 3 cut(s) 104, 166, 185
RsaNI GTAC 3 cut(s) 103, 165, 184
Rsr2I CGGWCCG 2 cut(s) 71, 97
RsrII CGGWCCG 2 cut(s) 71, 97
SacI GAGCTC 1 cut(s) 414
SatI GCNGC 3 cut(s) 77, 156, 240
Sau3AI GATC 2 cut(s) 114, 207
Sau96I GGNCC 3 cut(s) 71, 97, 128
ScrFI CCNGG 2 cut(s) 30, 81
SduI GDGCHC 1 cut(s) 414
SetI ASST 5 cut(s) 137, 170, 274, 345, 414
SfaNI GCATC 1 cut(s) 381
SfcI CTRYAG 1 cut(s) 62
SinI GGWCC 3 cut(s) 71, 97, 128
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 1 cut(s) 349
SsiI CCGC 5 cut(s) 74, 77, 156, 211, 415
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 2 cut(s) 28, 79
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 1 cut(s) 345
TaqI TCGA 1 cut(s) 315
TasI AATT 1 cut(s) 349
TauI GCSGC 2 cut(s) 79, 158
TfiI GAWTC 1 cut(s) 87
TscAI CASTG 2 cut(s) 18, 144
TseI GCWGC 1 cut(s) 239
TspDTI ATGAA 2 cut(s) 297, 350
TspRI CASTG 2 cut(s) 18, 144
VpaK11BI GGWCC 3 cut(s) 71, 97, 128
XceI RCATGY 2 cut(s) 55, 184
XmiI GTMKAC 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.