Rh4CG075400

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0011936
Physical Location & Seq
Reverse (-)
13808994 .. 13812596
3603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG075400.1

Sequence Viewer

Length: 432 bp
ATGACGGAGGCGATGATTAGAAGGAAGCCAGGGATGGCGAGCGTGAAGGACATGCCGGTTCTCCAAGACGGTCCACCACCGGGCGGGTTCCCTCCTGCCCGATACGCCCGTCGGATCCCCAACAAGGGTCCCAGCGCCGTCGCCATCTTCCTCGCCGCCTTCGGTACCTTCTCCTGGGGCATGTACCAGGTCGGCAAGGGCAACAAGATCCGAAGGGCACTAAAGGAAGAAAAATATGCAGCTCGCAGAGCAATACTTCCGGTGCTTCAAGCTGAAGAAGACGAAAGATTTGTCAAAGAGTGGAAAAAGTATCTTGATTATGAAGCTGAGGTTATGAAGGATGTTCCTAACTGGAAAGTTGGTGAGAGTGTCTACAACTCTGGGAGATGGATGCCCCCAGCTACTGGCGAGCTGCGCCCTGAGGTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
GRIM-19

Protein Analysis

143

Amino Acids

16.26

Weight (kDa)

9.69

Isoelectric Point (pI)

71.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRIM-19 PF06212 20 - 138 4.4e-40 GRIM-19 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011936)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04630 AT2G33220 AT2G33220
fragaria_vesca FvH4_4g05410
malus_domestica MD13G1228700.v1.1 MD16G1234000.v1.1
prunus_persica Prupe.1G056200_v2.0.a1
pyrus_communis pycom13g20200 pycom16g19500
rosa_chinensis RchiOBHm_Chr4g0396561
rosa_laevigata RLG00000009524
rosa_multiflora Rmu_sc0001781.1_g000004
rosa_roxburghii Rroxscaffold_5G00341410
rosa_rugosa Rorug03G0349200
rosa_samantha Rh4AG069900 Rh4BG067500 Rh4CG075400 Rh4DG065300
rosa_wichuraiana Rw4G005600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 1 cut(s) 404
AccI GTMKAC 1 cut(s) 372
AciI CCGC 2 cut(s) 84, 156
AclWI GGATC 3 cut(s) 109, 122, 202
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 2 cut(s) 166, 185
AfiI CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
AgsI TTSAA 1 cut(s) 269
AjnI CCWGG 3 cut(s) 28, 173, 186
AluBI AGCT 5 cut(s) 242, 272, 326, 401, 412
AluI AGCT 5 cut(s) 242, 272, 326, 401, 412
AlwI GGATC 3 cut(s) 109, 122, 202
AlwNI CAGNNNCTG 1 cut(s) 404
ApeKI GCWGC 2 cut(s) 239, 412
ArsI GACNNNNNNTTYG 4 cut(s) 272, 276, 304, 308
Asp718I GGTACC 1 cut(s) 164
AspLEI GCGC 2 cut(s) 137, 417
AspS9I GGNCC 2 cut(s) 71, 128
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 1 cut(s) 374
AvaII GGWCC 2 cut(s) 71, 128
AxyI CCTNAGG 1 cut(s) 420
BaeGI GKGCMC 1 cut(s) 220
BaeI ACNNNNGTAYC 2 cut(s) 94, 127
BamHI GGATCC 1 cut(s) 114
BanI GGYRCC 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 285
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 251, 399
BccI CCATC 3 cut(s) 28, 152, 381
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 3 cut(s) 30, 175, 188
BcnI CCSGG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 3 cut(s) 156, 240, 413
BlsI GCNGC 3 cut(s) 157, 241, 414
Bme1390I CCNGG 4 cut(s) 30, 81, 175, 188
Bme18I GGWCC 2 cut(s) 71, 128
BmgT120I GGNCC 2 cut(s) 71, 128
BmiI GGNNCC 5 cut(s) 89, 116, 129, 130, 166
BmrFI CCNGG 4 cut(s) 30, 81, 175, 188
BmsI GCATC 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 29, 174
BsaWI WCCGGW 1 cut(s) 259
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 2 cut(s) 356, 409
Bse21I CCTNAGG 1 cut(s) 420
BseBI CCWGG 3 cut(s) 30, 175, 188
BseDI CCNNGG 2 cut(s) 29, 174
BseGI GGATG 3 cut(s) 39, 346, 396
BseLI CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
BseMII CTCAG 2 cut(s) 318, 411
BseNI ACTGG 2 cut(s) 356, 409
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 2 cut(s) 251, 399
BseYI CCCAGC 2 cut(s) 131, 397
BshNI GGYRCC 1 cut(s) 164
BsiSI CCGG 3 cut(s) 56, 80, 260
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 6 cut(s) 80, 83, 124, 125, 174, 404
BsmFI GGGAC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 2 cut(s) 114, 207
BspACI CCGC 2 cut(s) 84, 156
BspCNI CTCAG 2 cut(s) 319, 412
BspLI GGNNCC 5 cut(s) 89, 116, 129, 130, 166
BspPI GGATC 3 cut(s) 109, 122, 202
BspT107I GGYRCC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 2 cut(s) 356, 409
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 29, 174
BssMI GATC 2 cut(s) 114, 207
Bst2UI CCWGG 3 cut(s) 30, 175, 188
Bst4CI ACNGT 1 cut(s) 71
BstC8I GCNNGC 3 cut(s) 40, 244, 410
BstDEI CTNAG 2 cut(s) 327, 420
BstF5I GGATG 3 cut(s) 39, 346, 396
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 2 cut(s) 137, 417
BstKTI GATC 2 cut(s) 117, 210
BstMBI GATC 2 cut(s) 114, 207
BstMWI GCNNNNNNNGC 3 cut(s) 104, 248, 414
BstNI CCWGG 3 cut(s) 30, 175, 188
BstNSI RCATGY 2 cut(s) 55, 184
BstSCI CCNGG 4 cut(s) 28, 79, 173, 186
BstSLI GKGCMC 1 cut(s) 220
BstV1I GCAGC 2 cut(s) 251, 399
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 2 cut(s) 114, 207
BstYI RGATCY 2 cut(s) 114, 207
Bsu36I CCTNAGG 1 cut(s) 420
BtgZI GCGATG 1 cut(s) 26
BtsCI GGATG 3 cut(s) 39, 346, 396
Cac8I GCNNGC 3 cut(s) 40, 244, 410
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 2 cut(s) 137, 417
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 71, 128
CsiI ACCWGGT 1 cut(s) 186
Csp6I GTAC 2 cut(s) 165, 184
CviAII CATG 2 cut(s) 52, 181
CviJI RGCY 6 cut(s) 28, 242, 272, 326, 401, 412
CviKI_1 RGCY 6 cut(s) 28, 242, 272, 326, 401, 412
CviQI GTAC 2 cut(s) 165, 184
DdeI CTNAG 2 cut(s) 327, 420
DpnI GATC 2 cut(s) 116, 209
DpnII GATC 2 cut(s) 114, 207
Eco47I GGWCC 2 cut(s) 71, 128
Eco57I CTGAAG 1 cut(s) 294
Eco81I CCTNAGG 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 28, 173, 186
FaeI CATG 2 cut(s) 55, 184
FaiI YATR 5 cut(s) 53, 182, 237, 321, 335
FaqI GGGAC 1 cut(s) 114
FatI CATG 2 cut(s) 51, 180
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 156, 240, 413
FokI GGATG 3 cut(s) 46, 353, 403
Fsp4HI GCNGC 3 cut(s) 156, 240, 413
GlaI GCGC 2 cut(s) 136, 416
GluI GCNGC 3 cut(s) 156, 240, 413
GsaI CCCAGC 2 cut(s) 135, 401
HaeII RGCGCY 1 cut(s) 138
HapII CCGG 3 cut(s) 56, 80, 260
HhaI GCGC 2 cut(s) 137, 417
Hin1II CATG 2 cut(s) 55, 184
Hin6I GCGC 2 cut(s) 135, 415
HinP1I GCGC 2 cut(s) 135, 415
HpaII CCGG 3 cut(s) 56, 80, 260
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 74, 373
Hpy188I TCNGA 2 cut(s) 114, 212
Hpy188III TCNNGA 1 cut(s) 314
Hpy8I GTNNAC 2 cut(s) 74, 373
Hpy99I CGWCG 2 cut(s) 114, 143
HpyAV CCTTC 6 cut(s) 15, 40, 169, 178, 207, 331
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 248, 414
HpyF3I CTNAG 2 cut(s) 327, 420
Hsp92II CATG 2 cut(s) 55, 184
HspAI GCGC 2 cut(s) 135, 415
KflI GGGWCCC 1 cut(s) 128
KpnI GGTACC 1 cut(s) 168
Kzo9I GATC 2 cut(s) 114, 207
Lsp1109I GCAGC 2 cut(s) 251, 399
LweI GCATC 1 cut(s) 381
MabI ACCWGGT 1 cut(s) 186
MalI GATC 2 cut(s) 116, 209
MboI GATC 2 cut(s) 114, 207
MboII GAAGA 4 cut(s) 139, 239, 287, 290
MflI RGATCY 2 cut(s) 114, 207
MhlI GDGCHC 1 cut(s) 220
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 4 cut(s) 102, 161, 322, 415
MspI CCGG 3 cut(s) 56, 80, 260
MspR9I CCNGG 4 cut(s) 30, 81, 175, 188
MvaI CCWGG 3 cut(s) 30, 175, 188
MwoI GCNNNNNNNGC 3 cut(s) 104, 248, 414
NciI CCSGG 1 cut(s) 81
NdeII GATC 2 cut(s) 114, 207
NlaIII CATG 2 cut(s) 55, 184
NlaIV GGNNCC 5 cut(s) 89, 116, 129, 130, 166
NspI RCATGY 2 cut(s) 55, 184
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 3 cut(s) 157, 241, 414
PpuMI RGGWCCY 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 3 cut(s) 28, 173, 186
PspFI CCCAGC 2 cut(s) 131, 397
PspGI CCWGG 3 cut(s) 28, 173, 186
PspN4I GGNNCC 5 cut(s) 89, 116, 129, 130, 166
PspPI GGNCC 2 cut(s) 71, 128
PspPPI RGGWCCY 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 2 cut(s) 114, 207
RsaI GTAC 2 cut(s) 166, 185
RsaNI GTAC 2 cut(s) 165, 184
SatI GCNGC 3 cut(s) 156, 240, 413
Sau3AI GATC 2 cut(s) 114, 207
Sau96I GGNCC 2 cut(s) 71, 128
ScrFI CCNGG 4 cut(s) 30, 81, 175, 188
SduI GDGCHC 1 cut(s) 220
SetI ASST 9 cut(s) 170, 192, 244, 274, 328, 333, 403, 414, 426
SexAI ACCWGGT 1 cut(s) 186
SfaNI GCATC 1 cut(s) 381
SinI GGWCC 2 cut(s) 71, 128
SsiI CCGC 2 cut(s) 84, 156
StyD4I CCNGG 4 cut(s) 28, 79, 173, 186
TaaI ACNGT 1 cut(s) 71
TauI GCSGC 1 cut(s) 158
TseI GCWGC 2 cut(s) 239, 412
TspDTI ATGAA 2 cut(s) 336, 350
TspGWI ACGGA 1 cut(s) 20
Van91I CCANNNNNTGG 1 cut(s) 404
VpaK11BI GGWCC 2 cut(s) 71, 128
XceI RCATGY 2 cut(s) 55, 184
XmiI GTMKAC 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.