FvH4_4g05880

voltage-gated potassium channel subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016125
Physical Location & Seq
Forward (+)
5266369 .. 5270607
4239 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05880.t1

Sequence Viewer

Length: 1002 bp
ATGACGTCATCGAACATGCAGTACAACAGCCTGGGCCGTTCGGGGCTGAAGGTGAGCCAGCTCTCATACGGCGCGTGGGTGAGCTTCGGGAACCAGCTGGACGTGAAGGAGGCGAAGTCGCTCCTCCAGTGCTGCCGTGATCACGGCGTCAACTTCTTCGACAACGCCGAGGTCTACGCGAGCGGCCGCGCCGAGGAGATCATGGGCCAGGCGATACGTGAGCTGGGGTGGAAGCGCTCCGACATCGTGGTCTCGACCAAGATCTTCTGGGGCGGGCCCGGCCCGAACGACAAGGGGCTGTCACGGAAGCACGTCGTGGAAGGGACAAAGGCGTCGTTGAAGAGGTTGGATATGGAGTACGTGGACCTTATTTATTGTCACCGGCCGGACTCGACGACGCCGATCGAGGAGACGGTGAGGGCGATGAATTATGTGATTGATAAGGGGTGGGCGTTTTATTGGGGGACTAGTGAGTGGTCGGCGCAGCAGATCACTGAGGCTTGGGGGATTGCTGAAAGGTTGGACTTGGTTGGTCCGGTTGTGGAGCAGCCCGAGTATAATCTCTTGTCCAGGCACAAGGTTGAGTCTGAGTTCCTTCCATTATACAGCAACTATGGCCTGGGTCTTACCACCTGGAGCCCACTTGCTTCCGGTGTGCTTACCGGAAAATATAACAAGGGAAATATACCACCTGATAGCCGATTTGCATTGGAGAACTACAAAAATCTAGCCAGTAGGTCACTGGTTGATGATGTGCTGAAGAAAGTTAATGGTCTGAAGCCAATTGCAGATGAATTGGGCGTGCCACTAGCTCAACTTGCAATTGCCTGGTGTGCTGCTAATCCTAATGTGTCATCAGTAATTACTGGTGCTACAAAGGAATCCCAGATCCAGGAGAACATGAAAGCAATTGATGTGATCCCTCTTTTGACTCCTGCTGTAATGGAGAAGATTGAGAATGTTGTTCAAAGCAAGCCGAAGCGTCCAGAATCGTATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

334

Amino Acids

37.01

Weight (kDa)

6.47

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 21 - 321 2.7e-64 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04690
fragaria_vesca FvH4_4g05880
malus_domestica MD13G1231200.v1.1 MD16G1235900.v1.1
prunus_persica Prupe.1G061100_v2.0.a1
pyrus_communis pycom13g20400 pycom16g19730
rosa_chinensis RchiOBHm_Chr4g0396981
rosa_laevigata RLG00000009462
rosa_multiflora Rmu_sc0002963.1_g000017 Rmu_sc0022770.1_g000004
rosa_roxburghii Rroxscaffold_5G00341970
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4BG072200
rosa_wichuraiana Rw4G006080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 248, 331
AatII GACGTC 1 cut(s) 8
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 174
AccII CGCG 3 cut(s) 74, 179, 189
AciI CCGC 3 cut(s) 183, 187, 273
AclWI GGATC 2 cut(s) 883, 913
AcoI YGGCCR 2 cut(s) 184, 383
AcuI CTGAAG 3 cut(s) 68, 779, 797
AcyI GRCGYC 4 cut(s) 5, 147, 332, 398
AdeI CACNNNGTG 1 cut(s) 316
AfaI GTAC 2 cut(s) 23, 359
AfeI AGCGCT 1 cut(s) 236
AfiI CCNNNNNNNGG 2 cut(s) 193, 892
AgsI TTSAA 2 cut(s) 340, 968
AhlI ACTAGT 1 cut(s) 467
AjiI CACGTC 2 cut(s) 103, 313
AjnI CCWGG 7 cut(s) 30, 207, 569, 618, 632, 827, 891
AluBI AGCT 5 cut(s) 61, 84, 97, 223, 812
AluI AGCT 5 cut(s) 61, 84, 97, 223, 812
Alw26I GTCTC 2 cut(s) 256, 404
AlwI GGATC 2 cut(s) 883, 913
Ama87I CYCGRG 1 cut(s) 551
Aor51HI AGCGCT 1 cut(s) 236
AoxI GGCC 7 cut(s) 34, 184, 205, 275, 280, 383, 616
ApaI GGGCCC 1 cut(s) 279
ApeKI GCWGC 4 cut(s) 132, 484, 547, 836
AspLEI GCGC 4 cut(s) 74, 191, 237, 484
AspS9I GGNCC 7 cut(s) 34, 205, 275, 276, 281, 364, 533
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 4 cut(s) 64, 91, 371, 427
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 2 cut(s) 364, 533
BaeGI GKGCMC 1 cut(s) 279
BanII GRGCYC 2 cut(s) 279, 641
BbvI GCAGC 4 cut(s) 119, 496, 559, 823
BceAI ACGGC 4 cut(s) 21, 85, 120, 160
BciT130I CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 2 cut(s) 256, 404
BcuI ACTAGT 1 cut(s) 467
BfaI CTAG 3 cut(s) 468, 728, 809
BfoI RGCGCY 1 cut(s) 238
BglII AGATCT 1 cut(s) 261
BisI GCNGC 6 cut(s) 133, 184, 187, 485, 548, 837
BlsI GCNGC 6 cut(s) 134, 185, 188, 486, 549, 838
Bme1390I CCNGG 8 cut(s) 32, 209, 279, 571, 620, 634, 829, 893
Bme18I GGWCC 2 cut(s) 364, 533
BmeT110I CYCGRG 1 cut(s) 551
BmgBI CACGTC 2 cut(s) 103, 313
BmgT120I GGNCC 7 cut(s) 34, 205, 275, 276, 281, 364, 533
BmiI GGNNCC 3 cut(s) 92, 277, 638
BmrFI CCNGG 8 cut(s) 32, 209, 279, 571, 620, 634, 829, 893
BpmI CTGGAG 2 cut(s) 110, 655
BpuMI CCSGG 1 cut(s) 279
BsaAI YACGTR 2 cut(s) 218, 361
BsaHI GRCGYC 4 cut(s) 5, 147, 332, 398
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 4 cut(s) 31, 168, 192, 619
BsaWI WCCGGW 3 cut(s) 535, 650, 662
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 892
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 4 cut(s) 127, 732, 747, 871
BseBI CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
BseDI CCNNGG 4 cut(s) 31, 168, 192, 619
BseLI CCNNNNNNNGG 2 cut(s) 193, 892
BseMII CTCAG 2 cut(s) 486, 579
BseNI ACTGG 4 cut(s) 127, 732, 747, 871
BseRI GAGGAG 3 cut(s) 113, 209, 422
BseSI GKGCMC 1 cut(s) 279
BseX3I CGGCCG 2 cut(s) 184, 383
BseXI GCAGC 4 cut(s) 119, 496, 559, 823
BseYI CCCAGC 1 cut(s) 223
Bsh1236I CGCG 3 cut(s) 74, 179, 189
Bsh1285I CGRYCG 3 cut(s) 187, 386, 405
BshFI GGCC 7 cut(s) 36, 186, 207, 277, 282, 385, 618
BsiEI CGRYCG 3 cut(s) 187, 386, 405
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 6 cut(s) 279, 382, 386, 536, 651, 663
BslFI GGGAC 2 cut(s) 337, 478
BslI CCNNNNNNNGG 2 cut(s) 193, 892
BsmAI GTCTC 2 cut(s) 256, 404
BsmBI CGTCTC 1 cut(s) 404
BsmFI GGGAC 2 cut(s) 337, 478
BsnI GGCC 7 cut(s) 36, 186, 207, 277, 282, 385, 618
Bso31I GGTCTC 1 cut(s) 256
BsoBI CYCGRG 1 cut(s) 551
Bsp120I GGGCCC 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 279, 641
Bsp143I GATC 7 cut(s) 139, 198, 261, 402, 489, 888, 918
BspACI CCGC 3 cut(s) 183, 187, 273
BspANI GGCC 7 cut(s) 36, 186, 207, 277, 282, 385, 618
BspCNI CTCAG 2 cut(s) 487, 580
BspFNI CGCG 3 cut(s) 74, 179, 189
BspLI GGNNCC 3 cut(s) 92, 277, 638
BspPI GGATC 2 cut(s) 883, 913
BspTNI GGTCTC 1 cut(s) 256
BsrBI CCGCTC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 4 cut(s) 127, 732, 747, 871
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 4 cut(s) 31, 168, 192, 619
BssMI GATC 7 cut(s) 139, 198, 261, 402, 489, 888, 918
BssNI GRCGYC 4 cut(s) 5, 147, 332, 398
Bst2UI CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 1 cut(s) 335
BstACI GRCGYC 4 cut(s) 5, 147, 332, 398
BstBAI YACGTR 2 cut(s) 218, 361
BstC8I GCNNGC 5 cut(s) 59, 181, 275, 803, 974
BstDEI CTNAG 2 cut(s) 495, 588
BstFNI CGCG 3 cut(s) 74, 179, 189
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 4 cut(s) 74, 191, 237, 484
BstKTI GATC 7 cut(s) 142, 201, 264, 405, 492, 891, 921
BstMAI GTCTC 2 cut(s) 256, 404
BstMBI GATC 7 cut(s) 139, 198, 261, 402, 489, 888, 918
BstMCI CGRYCG 3 cut(s) 187, 386, 405
BstMWI GCNNNNNNNGC 4 cut(s) 279, 615, 818, 833
BstNI CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 8 cut(s) 30, 207, 277, 569, 618, 632, 827, 891
BstSLI GKGCMC 1 cut(s) 279
BstUI CGCG 3 cut(s) 74, 179, 189
BstV1I GCAGC 4 cut(s) 119, 496, 559, 823
BstX2I RGATCY 2 cut(s) 261, 888
BstYI RGATCY 2 cut(s) 261, 888
BstZI CGGCCG 2 cut(s) 184, 383
BsuRI GGCC 7 cut(s) 36, 186, 207, 277, 282, 385, 618
BtgZI GCGATG 1 cut(s) 437
BtrI CACGTC 2 cut(s) 103, 313
BtsIMutI CAGTG 3 cut(s) 134, 492, 740
Cac8I GCNNGC 5 cut(s) 59, 181, 275, 803, 974
CciNI GCGGCCGC 1 cut(s) 184
CfoI GCGC 4 cut(s) 74, 191, 237, 484
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 7 cut(s) 34, 205, 275, 276, 281, 364, 533
CseI GACGC 4 cut(s) 136, 321, 406, 971
Csp6I GTAC 2 cut(s) 22, 358
CviAII CATG 3 cut(s) 16, 202, 901
CviQI GTAC 2 cut(s) 22, 358
DdeI CTNAG 2 cut(s) 495, 588
DpnI GATC 7 cut(s) 141, 200, 263, 404, 491, 890, 920
DpnII GATC 7 cut(s) 139, 198, 261, 402, 489, 888, 918
DraIII CACNNNGTG 1 cut(s) 316
DrdI GACNNNNNNGTC 2 cut(s) 248, 331
DseDI GACNNNNNNGTC 2 cut(s) 248, 331
EaeI YGGCCR 2 cut(s) 184, 383
EagI CGGCCG 2 cut(s) 184, 383
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
EclXI CGGCCG 2 cut(s) 184, 383
Eco24I GRGCYC 2 cut(s) 279, 641
Eco31I GGTCTC 1 cut(s) 256
Eco47I GGWCC 2 cut(s) 364, 533
Eco47III AGCGCT 1 cut(s) 236
Eco52I CGGCCG 2 cut(s) 184, 383
Eco57I CTGAAG 3 cut(s) 68, 779, 797
Eco88I CYCGRG 1 cut(s) 551
EcoRII CCWGG 7 cut(s) 30, 207, 569, 618, 632, 827, 891
EcoT38I GRGCYC 2 cut(s) 279, 641
Esp3I CGTCTC 1 cut(s) 404
FaeI CATG 3 cut(s) 19, 205, 904
FaqI GGGAC 2 cut(s) 337, 478
FatI CATG 3 cut(s) 15, 201, 900
FauI CCCGC 1 cut(s) 266
FbaI TGATCA 1 cut(s) 139
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 6 cut(s) 133, 184, 187, 485, 548, 837
FriOI GRGCYC 2 cut(s) 279, 641
Fsp4HI GCNGC 6 cut(s) 133, 184, 187, 485, 548, 837
FspBI CTAG 3 cut(s) 468, 728, 809
GlaI GCGC 4 cut(s) 73, 190, 236, 483
GluI GCNGC 6 cut(s) 133, 184, 187, 485, 548, 837
GsaI CCCAGC 1 cut(s) 227
GsuI CTGGAG 2 cut(s) 110, 655
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 7 cut(s) 36, 186, 207, 277, 282, 385, 618
HapII CCGG 6 cut(s) 279, 382, 386, 536, 651, 663
HgaI GACGC 4 cut(s) 136, 321, 406, 971
HhaI GCGC 4 cut(s) 74, 191, 237, 484
Hin1I GRCGYC 4 cut(s) 5, 147, 332, 398
Hin1II CATG 3 cut(s) 19, 205, 904
Hin6I GCGC 4 cut(s) 72, 189, 235, 482
HinP1I GCGC 4 cut(s) 72, 189, 235, 482
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 5 cut(s) 389, 584, 881, 931, 989
HpaII CCGG 6 cut(s) 279, 382, 386, 536, 651, 663
HphI GGTGA 4 cut(s) 64, 91, 371, 427
Hpy166II GTNNAC 3 cut(s) 151, 175, 364
Hpy188I TCNGA 3 cut(s) 241, 589, 777
Hpy188III TCNNGA 3 cut(s) 88, 253, 986
Hpy8I GTNNAC 3 cut(s) 151, 175, 364
Hpy99I CGWCG 4 cut(s) 317, 337, 397, 400
HpyAV CCTTC 4 cut(s) 43, 100, 314, 605
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4IV ACGT 5 cut(s) 5, 102, 217, 312, 360
HpyCH4V TGCA 4 cut(s) 19, 707, 788, 821
HpyF10VI GCNNNNNNNGC 4 cut(s) 279, 615, 818, 833
HpyF3I CTNAG 2 cut(s) 495, 588
HpySE526I ACGT 5 cut(s) 5, 102, 217, 312, 360
Hsp92I GRCGYC 4 cut(s) 5, 147, 332, 398
Hsp92II CATG 3 cut(s) 19, 205, 904
HspAI GCGC 4 cut(s) 72, 189, 235, 482
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 7 cut(s) 139, 198, 261, 402, 489, 888, 918
LmnI GCTCC 4 cut(s) 126, 242, 544, 636
Lsp1109I GCAGC 4 cut(s) 119, 496, 559, 823
MaeI CTAG 3 cut(s) 468, 728, 809
MaeII ACGT 5 cut(s) 5, 102, 217, 312, 360
MaeIII GTNAC 3 cut(s) 300, 377, 738
MalI GATC 7 cut(s) 141, 200, 263, 404, 491, 890, 920
MbiI CCGCTC 1 cut(s) 183
MboI GATC 7 cut(s) 139, 198, 261, 402, 489, 888, 918
MboII GAAGA 5 cut(s) 148, 256, 352, 772, 961
MfeI CAATTG 3 cut(s) 783, 822, 909
MflI RGATCY 2 cut(s) 261, 888
MhlI GDGCHC 2 cut(s) 279, 641
MluCI AATT 6 cut(s) 427, 783, 794, 822, 861, 909
MlyI GAGTC 3 cut(s) 383, 593, 925
MmeI TCCRAC 3 cut(s) 264, 327, 501
MnlI CCTC 9 cut(s) 103, 134, 163, 187, 336, 400, 411, 490, 933
MseI TTAA 1 cut(s) 768
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 6 cut(s) 279, 382, 386, 536, 651, 663
MspR9I CCNGG 8 cut(s) 32, 209, 279, 571, 620, 634, 829, 893
MunI CAATTG 3 cut(s) 783, 822, 909
MvaI CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
MvnI CGCG 3 cut(s) 74, 179, 189
MwoI GCNNNNNNNGC 4 cut(s) 279, 615, 818, 833
NciI CCSGG 1 cut(s) 279
NdeII GATC 7 cut(s) 139, 198, 261, 402, 489, 888, 918
NlaIII CATG 3 cut(s) 19, 205, 904
NlaIV GGNNCC 3 cut(s) 92, 277, 638
NmeAIII GCCGAG 2 cut(s) 193, 217
NmuCI GTSAC 3 cut(s) 300, 377, 738
NotI GCGGCCGC 1 cut(s) 184
NspI RCATGY 1 cut(s) 19
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 398, 419
PfeI GAWTC 2 cut(s) 881, 989
PfoI TCCNGGA 1 cut(s) 891
PkrI GCNGC 6 cut(s) 134, 185, 188, 486, 549, 838
Ple19I CGATCG 1 cut(s) 405
PleI GAGTC 3 cut(s) 383, 592, 925
PpsI GAGTC 3 cut(s) 383, 592, 925
Ppu21I YACGTR 2 cut(s) 218, 361
Psp6I CCWGG 7 cut(s) 30, 207, 569, 618, 632, 827, 891
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 7 cut(s) 30, 207, 569, 618, 632, 827, 891
PspN4I GGNNCC 3 cut(s) 92, 277, 638
PspOMI GGGCCC 1 cut(s) 275
PspPI GGNCC 7 cut(s) 34, 205, 275, 276, 281, 364, 533
PsrI GAACNNNNNNTAC 1 cut(s) 37
PsuI RGATCY 2 cut(s) 261, 888
PvuI CGATCG 1 cut(s) 405
PvuII CAGCTG 1 cut(s) 97
RsaI GTAC 2 cut(s) 23, 359
RsaNI GTAC 2 cut(s) 22, 358
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 6 cut(s) 133, 184, 187, 485, 548, 837
Sau3AI GATC 7 cut(s) 139, 198, 261, 402, 489, 888, 918
Sau96I GGNCC 7 cut(s) 34, 205, 275, 276, 281, 364, 533
SchI GAGTC 3 cut(s) 383, 593, 925
ScrFI CCNGG 8 cut(s) 32, 209, 279, 571, 620, 634, 829, 893
SduI GDGCHC 2 cut(s) 279, 641
SinI GGWCC 2 cut(s) 364, 533
SpeI ACTAGT 1 cut(s) 467
Sse9I AATT 6 cut(s) 427, 783, 794, 822, 861, 909
SsiI CCGC 3 cut(s) 183, 187, 273
SspMI CTAG 3 cut(s) 468, 728, 809
StyD4I CCNGG 8 cut(s) 30, 207, 277, 569, 618, 632, 827, 891
TaaI ACNGT 1 cut(s) 415
TaiI ACGT 5 cut(s) 8, 105, 220, 315, 363
TaqI TCGA 5 cut(s) 11, 159, 254, 392, 405
TasI AATT 6 cut(s) 427, 783, 794, 822, 861, 909
TatI WGTACW 1 cut(s) 21
TauI GCSGC 2 cut(s) 186, 189
TfiI GAWTC 2 cut(s) 881, 989
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 3 cut(s) 134, 499, 747
TseFI GTSAC 3 cut(s) 300, 377, 738
TseI GCWGC 4 cut(s) 132, 484, 547, 836
Tsp45I GTSAC 3 cut(s) 300, 377, 738
TspDTI ATGAA 3 cut(s) 440, 807, 917
TspGWI ACGGA 1 cut(s) 319
TspRI CASTG 3 cut(s) 134, 499, 747
VpaK11BI GGWCC 2 cut(s) 364, 533
XceI RCATGY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 3 cut(s) 468, 728, 809
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.