MD16G1235900.v1.1

voltage-gated potassium channel subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016125
Physical Location & Seq
Reverse (-)
24513448 .. 24518435
4988 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1235900.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGGTGTCGAATAATATGCAGTACAACAACCTGGGCCGGTCCGGCCTCAAAGTGAGCCAGCTCTCCTACGGCGCCTGGGTCAGCTTCGGCAACCAGCTCGACGTCAAAGAGGCCAAGTCTCTGCTCCAGACCTGCCGCGACCACGGCGTCAACTTCTTCGACAACGCCGAGGTCTACGCCTCCGGCCGCGCCGAGGAGATCATGGGCCAGGCCATCCGCGAGCTCGGCTGGAAGCGCTCCGACGTCGTCCTCTCCACCAAGATCTTCTGGGGCGGCCCCGGACCCAACGACAAGGGCCTCTCCCGGAAGCACATCGTGGAGGGCACCAAGGCGTCGCTCAAGCGGATGGACACGGACTACGTGGATCTCATCTACTGCCACCGTCCCGACTCCACGACGCCGATCGAGGAGACGGTGAGGGCGATGAACTATGTGATCGATAAGGGGTGGGCTTTTTATTGGGGGACCAGTGAGTGGTCGGCGCAGCAGATCACGGAGGCTTGGGGGATCGCCGAGAGGTTGGATTTGGTGGGGCCCATTGTTGAGCAGCCTGAGTATAATTTGCTCTCCAGGCACAAGGTTGAGTCTGAGTTCCTCCCACTGTATACCAACTATGGCCTGGGACTTACCACTTGGAGTCCACTTGCTTCTGGTGTTCTCACTGGAAAATATAACAAGGGAAATATACCTCCTGATAGCCGATTTGCATTGGACAATTACAAAAATCTTGCTAGTCGATCACTGGTTGATGACGTGCTGAGAAAAGTTAATGGTCTTAAGCCAATTGCAGATGAATTAGGTGTACCATTAGCTCAACTAGCAATTGCGTGGTGTGCTGCTAATCCGAATGTCTCATCAGTTATTACTGGTGCTACAAAGGAGTCACAGGTCCAAGAGAACATGAAAGCTATTGATGTAATCCCTCTATTAACTCCTGCTGTCATGGAAAAGATTGAGGCTGTTGTTCAAAGCAAGCCAAAGCGTCCAGAATCCTTTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

334

Amino Acids

37.01

Weight (kDa)

6.47

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 21 - 321 4.7e-65 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04690
fragaria_vesca FvH4_4g05880
malus_domestica MD13G1231200.v1.1 MD16G1235900.v1.1
prunus_persica Prupe.1G061100_v2.0.a1
pyrus_communis pycom13g20400 pycom16g19730
rosa_chinensis RchiOBHm_Chr4g0396981
rosa_laevigata RLG00000009462
rosa_multiflora Rmu_sc0002963.1_g000017 Rmu_sc0022770.1_g000004
rosa_roxburghii Rroxscaffold_5G00341970
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4BG072200
rosa_wichuraiana Rw4G006080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AatII GACGTC 2 cut(s) 105, 246
Acc36I ACCTGC 1 cut(s) 140
AccB1I GGYRCC 2 cut(s) 71, 323
AccB7I CCANNNNNTGG 1 cut(s) 474
AccI GTMKAC 2 cut(s) 174, 605
AccII CGCG 3 cut(s) 138, 189, 219
AciI CCGC 5 cut(s) 136, 187, 217, 273, 343
AclWI GGATC 2 cut(s) 372, 515
AcoI YGGCCR 1 cut(s) 184
AcyI GRCGYC 6 cut(s) 72, 102, 147, 243, 332, 398
AdeI CACNNNGTG 1 cut(s) 316
AfaI GTAC 2 cut(s) 23, 804
AfeI AGCGCT 1 cut(s) 236
AfiI CCNNNNNNNGG 2 cut(s) 193, 474
AflII CTTAAG 1 cut(s) 776
AgsI TTSAA 1 cut(s) 968
AjiI CACGTC 1 cut(s) 754
AjnI CCWGG 5 cut(s) 30, 74, 207, 569, 618
AluBI AGCT 6 cut(s) 61, 84, 97, 223, 812, 908
AluI AGCT 6 cut(s) 61, 84, 97, 223, 812, 908
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 3 cut(s) 123, 404, 856
AlwI GGATC 2 cut(s) 372, 515
Aor51HI AGCGCT 1 cut(s) 236
ApaI GGGCCC 1 cut(s) 537
ApeKI GCWGC 3 cut(s) 484, 547, 836
AspLEI GCGC 4 cut(s) 74, 191, 237, 484
AsuC2I CCSGG 2 cut(s) 279, 304
AsuHPI GGTGA 1 cut(s) 427
AvaII GGWCC 4 cut(s) 39, 281, 465, 889
BaeGI GKGCMC 2 cut(s) 326, 537
BanI GGYRCC 2 cut(s) 71, 323
BanII GRGCYC 2 cut(s) 225, 537
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 496, 559, 823
BccI CCATC 2 cut(s) 221, 340
BceAI ACGGC 2 cut(s) 85, 160
BcgI CGANNNNNNTGC 3 cut(s) 32, 79, 113
BciT130I CCWGG 5 cut(s) 32, 76, 209, 571, 620
BcnI CCSGG 2 cut(s) 279, 304
BcoDI GTCTC 3 cut(s) 123, 404, 856
BfaI CTAG 2 cut(s) 732, 818
BfoI RGCGCY 2 cut(s) 75, 238
BfrI CTTAAG 1 cut(s) 776
BfuAI ACCTGC 1 cut(s) 140
BglI GCCNNNNNGGC 1 cut(s) 42
BglII AGATCT 1 cut(s) 261
BisI GCNGC 6 cut(s) 136, 187, 274, 485, 548, 837
BlsI GCNGC 6 cut(s) 137, 188, 275, 486, 549, 838
Bme1390I CCNGG 7 cut(s) 32, 76, 209, 279, 304, 571, 620
Bme18I GGWCC 4 cut(s) 39, 281, 465, 889
BmgBI CACGTC 1 cut(s) 754
BmiI GGNNCC 7 cut(s) 73, 277, 283, 325, 466, 534, 535
BmrFI CCNGG 7 cut(s) 32, 76, 209, 279, 304, 571, 620
BpmI CTGGAG 2 cut(s) 110, 553
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 2 cut(s) 279, 304
Bsa29I ATCGAT 1 cut(s) 438
BsaAI YACGTR 1 cut(s) 361
BsaHI GRCGYC 6 cut(s) 72, 102, 147, 243, 332, 398
BsaJI CCNNGG 8 cut(s) 31, 75, 142, 168, 192, 277, 327, 619
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 474
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 4 cut(s) 468, 667, 747, 871
BseBI CCWGG 5 cut(s) 32, 76, 209, 571, 620
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 8 cut(s) 31, 75, 142, 168, 192, 277, 327, 619
BseGI GGATG 2 cut(s) 213, 351
BseLI CCNNNNNNNGG 2 cut(s) 193, 474
BseMII CTCAG 3 cut(s) 543, 579, 749
BseNI ACTGG 4 cut(s) 468, 667, 747, 871
BseRI GAGGAG 2 cut(s) 209, 422
BseSI GKGCMC 2 cut(s) 326, 537
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 3 cut(s) 496, 559, 823
Bsh1236I CGCG 3 cut(s) 138, 189, 219
Bsh1285I CGRYCG 2 cut(s) 187, 405
BshNI GGYRCC 2 cut(s) 71, 323
BshVI ATCGAT 1 cut(s) 438
BsiEI CGRYCG 2 cut(s) 187, 405
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 5 cut(s) 37, 42, 183, 279, 304
BslFI GGGAC 3 cut(s) 369, 478, 636
BslI CCNNNNNNNGG 2 cut(s) 193, 474
BsmAI GTCTC 3 cut(s) 123, 404, 856
BsmBI CGTCTC 1 cut(s) 404
BsmFI GGGAC 3 cut(s) 369, 478, 636
Bsp120I GGGCCC 1 cut(s) 533
Bsp1286I GDGCHC 3 cut(s) 225, 326, 537
Bsp143I GATC 8 cut(s) 198, 261, 364, 402, 435, 489, 507, 737
BspACI CCGC 5 cut(s) 136, 187, 217, 273, 343
BspCNI CTCAG 3 cut(s) 544, 580, 750
BspDI ATCGAT 1 cut(s) 438
BspFNI CGCG 3 cut(s) 138, 189, 219
BspLI GGNNCC 7 cut(s) 73, 277, 283, 325, 466, 534, 535
BspMI ACCTGC 1 cut(s) 140
BspPI GGATC 2 cut(s) 372, 515
BspT107I GGYRCC 2 cut(s) 71, 323
BspTI CTTAAG 1 cut(s) 776
BsrFI RCCGGY 1 cut(s) 36
BsrI ACTGG 4 cut(s) 468, 667, 747, 871
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 8 cut(s) 31, 75, 142, 168, 192, 277, 327, 619
BssMI GATC 8 cut(s) 198, 261, 364, 402, 435, 489, 507, 737
BssNAI GTATAC 1 cut(s) 606
BssNI GRCGYC 6 cut(s) 72, 102, 147, 243, 332, 398
BssT1I CCWWGG 1 cut(s) 327
Bst1107I GTATAC 1 cut(s) 606
Bst2UI CCWGG 5 cut(s) 32, 76, 209, 571, 620
Bst4CI ACNGT 3 cut(s) 383, 415, 603
BstACI GRCGYC 6 cut(s) 72, 102, 147, 243, 332, 398
BstAFI CTTAAG 1 cut(s) 776
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 3 cut(s) 59, 221, 974
BstDEI CTNAG 3 cut(s) 552, 588, 758
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 2 cut(s) 213, 351
BstFNI CGCG 3 cut(s) 138, 189, 219
BstH2I RGCGCY 2 cut(s) 75, 238
BstHHI GCGC 4 cut(s) 74, 191, 237, 484
BstKTI GATC 8 cut(s) 201, 264, 367, 405, 438, 492, 510, 740
BstMAI GTCTC 3 cut(s) 123, 404, 856
BstMBI GATC 8 cut(s) 198, 261, 364, 402, 435, 489, 507, 737
BstMCI CGRYCG 2 cut(s) 187, 405
BstMWI GCNNNNNNNGC 7 cut(s) 42, 144, 225, 234, 571, 818, 833
BstNI CCWGG 5 cut(s) 32, 76, 209, 571, 620
BstSCI CCNGG 7 cut(s) 30, 74, 207, 277, 302, 569, 618
BstSLI GKGCMC 2 cut(s) 326, 537
BstUI CGCG 3 cut(s) 138, 189, 219
BstV1I GCAGC 3 cut(s) 496, 559, 823
BstX2I RGATCY 2 cut(s) 261, 364
BstYI RGATCY 2 cut(s) 261, 364
BstZ17I GTATAC 1 cut(s) 606
BstZI CGGCCG 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 438
BsuTUI ATCGAT 1 cut(s) 438
BtgI CCRYGG 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 437
BtrI CACGTC 1 cut(s) 754
BtsCI GGATG 2 cut(s) 213, 351
BtsIMutI CAGTG 4 cut(s) 475, 599, 660, 740
BveI ACCTGC 1 cut(s) 140
Cac8I GCNNGC 3 cut(s) 59, 221, 974
CfoI GCGC 4 cut(s) 74, 191, 237, 484
Cfr10I RCCGGY 1 cut(s) 36
ClaI ATCGAT 1 cut(s) 438
CpoI CGGWCCG 1 cut(s) 39
CseI GACGC 4 cut(s) 136, 321, 406, 971
Csp6I GTAC 2 cut(s) 22, 803
CspI CGGWCCG 1 cut(s) 39
CviAII CATG 3 cut(s) 202, 901, 943
CviQI GTAC 2 cut(s) 22, 803
DdeI CTNAG 3 cut(s) 552, 588, 758
DinI GGCGCC 1 cut(s) 73
DpnI GATC 8 cut(s) 200, 263, 366, 404, 437, 491, 509, 739
DpnII GATC 8 cut(s) 198, 261, 364, 402, 435, 489, 507, 737
DraIII CACNNNGTG 1 cut(s) 316
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
EaeI YGGCCR 1 cut(s) 184
EagI CGGCCG 1 cut(s) 184
Ecl136II GAGCTC 1 cut(s) 223
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 2 cut(s) 225, 537
Eco47I GGWCC 4 cut(s) 39, 281, 465, 889
Eco47III AGCGCT 1 cut(s) 236
Eco52I CGGCCG 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 295, 533
EcoRII CCWGG 5 cut(s) 30, 74, 207, 569, 618
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 2 cut(s) 225, 537
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 404
FaeI CATG 3 cut(s) 205, 904, 946
FaqI GGGAC 3 cut(s) 369, 478, 636
FatI CATG 3 cut(s) 201, 900, 942
FblI GTMKAC 2 cut(s) 174, 605
Fnu4HI GCNGC 6 cut(s) 136, 187, 274, 485, 548, 837
FokI GGATG 2 cut(s) 200, 358
FriOI GRGCYC 2 cut(s) 225, 537
Fsp4HI GCNGC 6 cut(s) 136, 187, 274, 485, 548, 837
FspBI CTAG 2 cut(s) 732, 818
GlaI GCGC 4 cut(s) 73, 190, 236, 483
GluI GCNGC 6 cut(s) 136, 187, 274, 485, 548, 837
GsuI CTGGAG 2 cut(s) 110, 553
HaeII RGCGCY 2 cut(s) 75, 238
HapII CCGG 5 cut(s) 37, 42, 183, 279, 304
HgaI GACGC 4 cut(s) 136, 321, 406, 971
HhaI GCGC 4 cut(s) 74, 191, 237, 484
Hin1I GRCGYC 6 cut(s) 72, 102, 147, 243, 332, 398
Hin1II CATG 3 cut(s) 205, 904, 946
Hin6I GCGC 4 cut(s) 72, 189, 235, 482
HinP1I GCGC 4 cut(s) 72, 189, 235, 482
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 5 cut(s) 389, 584, 637, 881, 989
HpaII CCGG 5 cut(s) 37, 42, 183, 279, 304
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 5 cut(s) 151, 175, 606, 641, 803
Hpy188I TCNGA 3 cut(s) 241, 589, 846
Hpy188III TCNNGA 4 cut(s) 127, 386, 692, 986
Hpy8I GTNNAC 5 cut(s) 151, 175, 606, 641, 803
Hpy99I CGWCG 5 cut(s) 104, 245, 248, 337, 400
HpyCH4III ACNGT 3 cut(s) 383, 415, 603
HpyCH4IV ACGT 4 cut(s) 102, 243, 360, 753
HpyCH4V TGCA 3 cut(s) 19, 707, 788
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 144, 225, 234, 571, 818, 833
HpyF3I CTNAG 3 cut(s) 552, 588, 758
HpySE526I ACGT 4 cut(s) 102, 243, 360, 753
Hsp92I GRCGYC 6 cut(s) 72, 102, 147, 243, 332, 398
Hsp92II CATG 3 cut(s) 205, 904, 946
HspAI GCGC 4 cut(s) 72, 189, 235, 482
KasI GGCGCC 1 cut(s) 71
Kzo9I GATC 8 cut(s) 198, 261, 364, 402, 435, 489, 507, 737
LmnI GCTCC 2 cut(s) 129, 242
Lsp1109I GCAGC 3 cut(s) 496, 559, 823
MaeI CTAG 2 cut(s) 732, 818
MaeII ACGT 4 cut(s) 102, 243, 360, 753
MaeIII GTNAC 1 cut(s) 882
MalI GATC 8 cut(s) 200, 263, 366, 404, 437, 491, 509, 739
MboI GATC 8 cut(s) 198, 261, 364, 402, 435, 489, 507, 737
MboII GAAGA 2 cut(s) 148, 256
MfeI CAATTG 2 cut(s) 783, 822
MflI RGATCY 2 cut(s) 261, 364
MhlI GDGCHC 3 cut(s) 225, 326, 537
MluCI AATT 5 cut(s) 559, 715, 783, 794, 822
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 4 cut(s) 383, 593, 646, 890
MmeI TCCRAC 2 cut(s) 264, 501
MseI TTAA 3 cut(s) 768, 777, 929
MspCI CTTAAG 1 cut(s) 776
MspI CCGG 5 cut(s) 37, 42, 183, 279, 304
MspR9I CCNGG 7 cut(s) 32, 76, 209, 279, 304, 571, 620
MunI CAATTG 2 cut(s) 783, 822
MvaI CCWGG 5 cut(s) 32, 76, 209, 571, 620
MvnI CGCG 3 cut(s) 138, 189, 219
MwoI GCNNNNNNNGC 7 cut(s) 42, 144, 225, 234, 571, 818, 833
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 2 cut(s) 279, 304
NdeII GATC 8 cut(s) 198, 261, 364, 402, 435, 489, 507, 737
NlaIII CATG 3 cut(s) 205, 904, 946
NlaIV GGNNCC 7 cut(s) 73, 277, 283, 325, 466, 534, 535
NmeAIII GCCGAG 4 cut(s) 193, 204, 217, 538
NmuCI GTSAC 1 cut(s) 882
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 419
PfeI GAWTC 1 cut(s) 989
PflFI GACNNNGTC 1 cut(s) 245
PflMI CCANNNNNTGG 1 cut(s) 474
PfoI TCCNGGA 1 cut(s) 302
PkrI GCNGC 6 cut(s) 137, 188, 275, 486, 549, 838
Ple19I CGATCG 1 cut(s) 405
PleI GAGTC 4 cut(s) 383, 592, 645, 889
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 4 cut(s) 383, 592, 645, 889
Ppu21I YACGTR 1 cut(s) 361
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 5 cut(s) 30, 74, 207, 569, 618
PspGI CCWGG 5 cut(s) 30, 74, 207, 569, 618
PspN4I GGNNCC 7 cut(s) 73, 277, 283, 325, 466, 534, 535
PspOMI GGGCCC 1 cut(s) 533
PsuI RGATCY 2 cut(s) 261, 364
PsyI GACNNNGTC 1 cut(s) 245
PvuI CGATCG 1 cut(s) 405
RsaI GTAC 2 cut(s) 23, 804
RsaNI GTAC 2 cut(s) 22, 803
Rsr2I CGGWCCG 1 cut(s) 39
RsrII CGGWCCG 1 cut(s) 39
SacI GAGCTC 1 cut(s) 225
SaqAI TTAA 3 cut(s) 768, 777, 929
SatI GCNGC 6 cut(s) 136, 187, 274, 485, 548, 837
Sau3AI GATC 8 cut(s) 198, 261, 364, 402, 435, 489, 507, 737
SchI GAGTC 4 cut(s) 383, 593, 646, 890
ScrFI CCNGG 7 cut(s) 32, 76, 209, 279, 304, 571, 620
SduI GDGCHC 3 cut(s) 225, 326, 537
SfiI GGCCNNNNNGGCC 1 cut(s) 42
SfoI GGCGCC 1 cut(s) 73
SinI GGWCC 4 cut(s) 39, 281, 465, 889
SmlI CTYRAG 2 cut(s) 338, 776
SmoI CTYRAG 2 cut(s) 338, 776
Sse9I AATT 5 cut(s) 559, 715, 783, 794, 822
SsiI CCGC 5 cut(s) 136, 187, 217, 273, 343
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 2 cut(s) 732, 818
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 7 cut(s) 30, 74, 207, 277, 302, 569, 618
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 3 cut(s) 383, 415, 603
TaiI ACGT 4 cut(s) 105, 246, 363, 756
TaqI TCGA 6 cut(s) 8, 99, 159, 405, 438, 736
TasI AATT 5 cut(s) 559, 715, 783, 794, 822
TatI WGTACW 1 cut(s) 21
TauI GCSGC 3 cut(s) 138, 189, 276
TfiI GAWTC 1 cut(s) 989
Tru1I TTAA 3 cut(s) 768, 777, 929
Tru9I TTAA 3 cut(s) 768, 777, 929
TscAI CASTG 4 cut(s) 475, 606, 667, 747
TseFI GTSAC 1 cut(s) 882
TseI GCWGC 3 cut(s) 484, 547, 836
Tsp45I GTSAC 1 cut(s) 882
TspDTI ATGAA 3 cut(s) 440, 807, 917
TspGWI ACGGA 2 cut(s) 368, 509
TspRI CASTG 4 cut(s) 475, 606, 667, 747
Tth111I GACNNNGTC 1 cut(s) 245
Van91I CCANNNNNTGG 1 cut(s) 474
Vha464I CTTAAG 1 cut(s) 776
VpaK11BI GGWCC 4 cut(s) 39, 281, 465, 889
XcmI CCANNNNNNNNNTGG 1 cut(s) 616
XmiI GTMKAC 2 cut(s) 174, 605
XspI CTAG 2 cut(s) 732, 818
ZraI GACGTC 2 cut(s) 103, 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.