RLG00000009462

voltage-gated potassium channel subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0016125
Physical Location & Seq
Reverse (-)
53253217 .. 53256064
2848 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009462

Sequence Viewer

Length: 1002 bp
ATGACGTCATCAAACATGATTTACAACAACCTGGGGCGGTCGGGGCTGAAGGTGAGCCAGCTGTCGTACGGCGCGTGGGTGAGCTTCGGGAACCAGCTGGACGTGAAGGAGGCGAAGTCGCTGCTCCAGTGCTGCCGCGACCACGGCGTCAACTTCTTCGACAACGCCGAGGTCTACGCGAGCGGCCGCGCCGAGGAGATCATGGGCCAGGCCATACGCGAGCTGGGCTGGAAGCGCTCCGACATCGTCGTCTCCACCAAGATCTTCTGGGGCGGGCCCGGCCCAAACGACAAGGGCCTCTCCCGGAAGCACATCGTAGAGGGCACCAAGGCCTCCCTCAAGAGACTCGATATGGAGTACGTCGACCTCATCTACTGTCACCGGCCGGACTCCACCACGCCGATCGAGGAGACGGTCAGGGCTATGAATTATGTGATTGATAAGGGCTGGGCTTTTTATTGGGGCACCAGTGAGTGGTCGGCCCAGCAGATCACCGAGGCTTGGGGAATTGCTGACAAGTTGGATTTGGTTGGTCCGATTGTGGAGCAGCCGGAGTATAACTTGTTGTCCAGGCACAAGGTTGAGTCTGAGTTCCTCCCATTATACAGCAACCATGGCCTGGGTCTTACCACCTGGAGCCCACTTGCTTCTGGTGTGCTCACCGGAAAATATAACAAGGGAAATATACCACCTGATAGCCGATTTGCATTGGAGAACTACAAAAATCTAGCCAGTAGGTCACTGGTTGATGACGTGCTGAAGAAGGTTAATGGTCTGAAGCCAATTGCAGATGAGTTAGGTGTGCCACTAGCTCAACTTGCAATTGCCTGGTGTGCTGCTAATCCTAATGTGTCATCAGTTATTACTGGTGCTACAAAGGAATCCCAGATCCAGGAGAACATGAAAGCAATTGATGTGATCCCTCTTTTAACTCCTACTGTGATGGAGAAGATTGAGAATGTTGTTCAAAGCAGGCCGAAGCGTCCAGAATCATATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

334

Amino Acids

37.04

Weight (kDa)

6.55

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 21 - 321 1.8e-64 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04690
fragaria_vesca FvH4_4g05880
malus_domestica MD13G1231200.v1.1 MD16G1235900.v1.1
prunus_persica Prupe.1G061100_v2.0.a1
pyrus_communis pycom13g20400 pycom16g19730
rosa_chinensis RchiOBHm_Chr4g0396981
rosa_laevigata RLG00000009462
rosa_multiflora Rmu_sc0002963.1_g000017 Rmu_sc0022770.1_g000004
rosa_roxburghii Rroxscaffold_5G00341970
rosa_rugosa Rorug03G0346000
rosa_samantha Rh4BG072200
rosa_wichuraiana Rw4G006080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 146, 248
AatII GACGTC 1 cut(s) 8
AccB1I GGYRCC 2 cut(s) 323, 464
AccB7I CCANNNNNTGG 2 cut(s) 474, 619
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 2 cut(s) 174, 363
AccII CGCG 5 cut(s) 74, 138, 179, 189, 219
AciI CCGC 5 cut(s) 37, 136, 183, 187, 273
AclWI GGATC 2 cut(s) 883, 913
AcoI YGGCCR 2 cut(s) 184, 383
AcuI CTGAAG 3 cut(s) 68, 779, 797
AcyI GRCGYC 2 cut(s) 5, 147
AfaI GTAC 2 cut(s) 68, 359
AfeI AGCGCT 1 cut(s) 236
AfiI CCNNNNNNNGG 5 cut(s) 193, 474, 501, 619, 892
AgsI TTSAA 1 cut(s) 968
AjiI CACGTC 2 cut(s) 103, 754
AjnI CCWGG 7 cut(s) 30, 207, 569, 618, 632, 827, 891
AluBI AGCT 5 cut(s) 61, 84, 97, 223, 812
AluI AGCT 5 cut(s) 61, 84, 97, 223, 812
Alw21I GWGCWC 1 cut(s) 660
Alw26I GTCTC 3 cut(s) 256, 337, 404
AlwI GGATC 2 cut(s) 883, 913
Aor51HI AGCGCT 1 cut(s) 236
ApaI GGGCCC 1 cut(s) 279
ApeKI GCWGC 4 cut(s) 121, 132, 547, 836
AspLEI GCGC 3 cut(s) 74, 191, 237
AspS9I GGNCC 7 cut(s) 205, 275, 276, 281, 295, 481, 533
AsuC2I CCSGG 2 cut(s) 279, 304
AsuHPI GGTGA 5 cut(s) 64, 91, 371, 484, 652
AvaII GGWCC 1 cut(s) 533
BaeGI GKGCMC 3 cut(s) 279, 326, 467
BanI GGYRCC 2 cut(s) 323, 464
BanII GRGCYC 2 cut(s) 279, 641
Bbv12I GWGCWC 1 cut(s) 660
BbvI GCAGC 4 cut(s) 108, 119, 559, 823
BccI CCATC 1 cut(s) 937
BceAI ACGGC 2 cut(s) 85, 160
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BciT130I CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
BcnI CCSGG 2 cut(s) 279, 304
BcoDI GTCTC 3 cut(s) 256, 337, 404
BfaI CTAG 2 cut(s) 728, 809
BfoI RGCGCY 1 cut(s) 238
BglII AGATCT 1 cut(s) 261
BisI GCNGC 7 cut(s) 122, 133, 136, 184, 187, 548, 837
BlsI GCNGC 7 cut(s) 123, 134, 137, 185, 188, 549, 838
Bme1390I CCNGG 9 cut(s) 32, 209, 279, 304, 571, 620, 634, 829, 893
Bme18I GGWCC 1 cut(s) 533
BmgBI CACGTC 2 cut(s) 103, 754
BmgT120I GGNCC 7 cut(s) 205, 275, 276, 281, 295, 481, 533
BmiI GGNNCC 5 cut(s) 92, 277, 325, 466, 638
BmrFI CCNGG 9 cut(s) 32, 209, 279, 304, 571, 620, 634, 829, 893
BpmI CTGGAG 2 cut(s) 110, 655
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 2 cut(s) 279, 304
BsaHI GRCGYC 2 cut(s) 5, 147
BsaJI CCNNGG 8 cut(s) 31, 142, 168, 192, 327, 495, 613, 619
BsaWI WCCGGW 1 cut(s) 662
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 5 cut(s) 193, 474, 501, 619, 892
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 5 cut(s) 127, 468, 732, 747, 871
BseBI CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
BseDI CCNNGG 8 cut(s) 31, 142, 168, 192, 327, 495, 613, 619
BseLI CCNNNNNNNGG 5 cut(s) 193, 474, 501, 619, 892
BseMII CTCAG 1 cut(s) 579
BseNI ACTGG 5 cut(s) 127, 468, 732, 747, 871
BseRI GAGGAG 2 cut(s) 209, 422
BseSI GKGCMC 3 cut(s) 279, 326, 467
BseX3I CGGCCG 2 cut(s) 184, 383
BseXI GCAGC 4 cut(s) 108, 119, 559, 823
BseYI CCCAGC 3 cut(s) 223, 447, 483
Bsh1236I CGCG 5 cut(s) 74, 138, 179, 189, 219
Bsh1285I CGRYCG 4 cut(s) 41, 187, 386, 405
BshNI GGYRCC 2 cut(s) 323, 464
BsiEI CGRYCG 4 cut(s) 41, 187, 386, 405
BsiHKAI GWGCWC 1 cut(s) 660
BsiSI CCGG 6 cut(s) 279, 304, 382, 386, 551, 663
BsiWI CGTACG 1 cut(s) 66
BslI CCNNNNNNNGG 5 cut(s) 193, 474, 501, 619, 892
BsmAI GTCTC 3 cut(s) 256, 337, 404
BsmBI CGTCTC 2 cut(s) 256, 404
Bsp120I GGGCCC 1 cut(s) 275
Bsp1286I GDGCHC 5 cut(s) 279, 326, 467, 641, 660
Bsp143I GATC 6 cut(s) 198, 261, 402, 489, 888, 918
Bsp19I CCATGG 1 cut(s) 613
BspACI CCGC 5 cut(s) 37, 136, 183, 187, 273
BspCNI CTCAG 1 cut(s) 580
BspFNI CGCG 5 cut(s) 74, 138, 179, 189, 219
BspLI GGNNCC 5 cut(s) 92, 277, 325, 466, 638
BspPI GGATC 2 cut(s) 883, 913
BspT107I GGYRCC 2 cut(s) 323, 464
BsrBI CCGCTC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 5 cut(s) 127, 468, 732, 747, 871
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 8 cut(s) 31, 142, 168, 192, 327, 495, 613, 619
BssMI GATC 6 cut(s) 198, 261, 402, 489, 888, 918
BssNI GRCGYC 2 cut(s) 5, 147
BssT1I CCWWGG 2 cut(s) 327, 613
Bst2UI CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
Bst4CI ACNGT 3 cut(s) 377, 415, 940
BstACI GRCGYC 2 cut(s) 5, 147
BstC8I GCNNGC 5 cut(s) 59, 181, 221, 275, 974
BstDEI CTNAG 1 cut(s) 588
BstDSI CCRYGG 2 cut(s) 142, 613
BstFNI CGCG 5 cut(s) 74, 138, 179, 189, 219
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 3 cut(s) 74, 191, 237
BstKTI GATC 6 cut(s) 201, 264, 405, 492, 891, 921
BstMAI GTCTC 3 cut(s) 256, 337, 404
BstMBI GATC 6 cut(s) 198, 261, 402, 489, 888, 918
BstMCI CGRYCG 4 cut(s) 41, 187, 386, 405
BstMWI GCNNNNNNNGC 8 cut(s) 43, 144, 225, 234, 279, 615, 818, 833
BstNI CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
BstSCI CCNGG 9 cut(s) 30, 207, 277, 302, 569, 618, 632, 827, 891
BstSLI GKGCMC 3 cut(s) 279, 326, 467
BstUI CGCG 5 cut(s) 74, 138, 179, 189, 219
BstV1I GCAGC 4 cut(s) 108, 119, 559, 823
BstX2I RGATCY 2 cut(s) 261, 888
BstYI RGATCY 2 cut(s) 261, 888
BstZI CGGCCG 2 cut(s) 184, 383
BtgI CCRYGG 2 cut(s) 142, 613
BtrI CACGTC 2 cut(s) 103, 754
BtsIMutI CAGTG 3 cut(s) 134, 475, 740
Cac8I GCNNGC 5 cut(s) 59, 181, 221, 275, 974
CciNI GCGGCCGC 1 cut(s) 184
CfoI GCGC 3 cut(s) 74, 191, 237
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 7 cut(s) 205, 275, 276, 281, 295, 481, 533
CseI GACGC 2 cut(s) 136, 971
Csp6I GTAC 2 cut(s) 67, 358
CviAII CATG 4 cut(s) 16, 202, 614, 901
CviQI GTAC 2 cut(s) 67, 358
DdeI CTNAG 1 cut(s) 588
DpnI GATC 6 cut(s) 200, 263, 404, 491, 890, 920
DpnII GATC 6 cut(s) 198, 261, 402, 489, 888, 918
DrdI GACNNNNNNGTC 2 cut(s) 146, 248
DseDI GACNNNNNNGTC 2 cut(s) 146, 248
EaeI YGGCCR 2 cut(s) 184, 383
EagI CGGCCG 2 cut(s) 184, 383
EclXI CGGCCG 2 cut(s) 184, 383
Eco130I CCWWGG 2 cut(s) 327, 613
Eco147I AGGCCT 1 cut(s) 332
Eco24I GRGCYC 2 cut(s) 279, 641
Eco47I GGWCC 1 cut(s) 533
Eco47III AGCGCT 1 cut(s) 236
Eco52I CGGCCG 2 cut(s) 184, 383
Eco57I CTGAAG 3 cut(s) 68, 779, 797
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 7 cut(s) 30, 207, 569, 618, 632, 827, 891
EcoT14I CCWWGG 2 cut(s) 327, 613
EcoT38I GRGCYC 2 cut(s) 279, 641
ErhI CCWWGG 2 cut(s) 327, 613
Esp3I CGTCTC 2 cut(s) 256, 404
FaeI CATG 4 cut(s) 19, 205, 617, 904
FatI CATG 4 cut(s) 15, 201, 613, 900
FauI CCCGC 1 cut(s) 266
FblI GTMKAC 2 cut(s) 174, 363
Fnu4HI GCNGC 7 cut(s) 122, 133, 136, 184, 187, 548, 837
FriOI GRGCYC 2 cut(s) 279, 641
Fsp4HI GCNGC 7 cut(s) 122, 133, 136, 184, 187, 548, 837
FspBI CTAG 2 cut(s) 728, 809
GlaI GCGC 3 cut(s) 73, 190, 236
GluI GCNGC 7 cut(s) 122, 133, 136, 184, 187, 548, 837
GsaI CCCAGC 3 cut(s) 227, 451, 487
GsuI CTGGAG 2 cut(s) 110, 655
HaeII RGCGCY 1 cut(s) 238
HapII CCGG 6 cut(s) 279, 304, 382, 386, 551, 663
HgaI GACGC 2 cut(s) 136, 971
HhaI GCGC 3 cut(s) 74, 191, 237
Hin1I GRCGYC 2 cut(s) 5, 147
Hin1II CATG 4 cut(s) 19, 205, 617, 904
Hin6I GCGC 3 cut(s) 72, 189, 235
HinP1I GCGC 3 cut(s) 72, 189, 235
HincII GTYRAC 2 cut(s) 151, 364
HindII GTYRAC 2 cut(s) 151, 364
HinfI GANTC 5 cut(s) 345, 389, 584, 881, 989
HpaII CCGG 6 cut(s) 279, 304, 382, 386, 551, 663
HphI GGTGA 5 cut(s) 64, 91, 371, 484, 652
Hpy166II GTNNAC 3 cut(s) 151, 175, 364
Hpy188I TCNGA 4 cut(s) 241, 537, 589, 777
Hpy188III TCNNGA 3 cut(s) 88, 340, 986
Hpy8I GTNNAC 3 cut(s) 151, 175, 364
Hpy99I CGWCG 2 cut(s) 251, 365
HpyAV CCTTC 3 cut(s) 43, 100, 757
HpyCH4III ACNGT 3 cut(s) 377, 415, 940
HpyCH4IV ACGT 4 cut(s) 5, 102, 360, 753
HpyCH4V TGCA 3 cut(s) 707, 788, 821
HpyF10VI GCNNNNNNNGC 8 cut(s) 43, 144, 225, 234, 279, 615, 818, 833
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 4 cut(s) 5, 102, 360, 753
Hsp92I GRCGYC 2 cut(s) 5, 147
Hsp92II CATG 4 cut(s) 19, 205, 617, 904
HspAI GCGC 3 cut(s) 72, 189, 235
Kzo9I GATC 6 cut(s) 198, 261, 402, 489, 888, 918
LmnI GCTCC 4 cut(s) 129, 242, 544, 636
Lsp1109I GCAGC 4 cut(s) 108, 119, 559, 823
MaeI CTAG 2 cut(s) 728, 809
MaeII ACGT 4 cut(s) 5, 102, 360, 753
MaeIII GTNAC 2 cut(s) 377, 738
MalI GATC 6 cut(s) 200, 263, 404, 491, 890, 920
MbiI CCGCTC 1 cut(s) 183
MboI GATC 6 cut(s) 198, 261, 402, 489, 888, 918
MboII GAAGA 4 cut(s) 148, 256, 772, 961
MfeI CAATTG 3 cut(s) 783, 822, 909
MflI RGATCY 2 cut(s) 261, 888
MhlI GDGCHC 5 cut(s) 279, 326, 467, 641, 660
MluCI AATT 5 cut(s) 427, 507, 783, 822, 909
MlyI GAGTC 3 cut(s) 339, 383, 593
MmeI TCCRAC 2 cut(s) 264, 501
MseI TTAA 2 cut(s) 768, 929
MslI CAYNNNNRTG 1 cut(s) 997
MspA1I CMGCKG 2 cut(s) 61, 97
MspI CCGG 6 cut(s) 279, 304, 382, 386, 551, 663
MspR9I CCNGG 9 cut(s) 32, 209, 279, 304, 571, 620, 634, 829, 893
MunI CAATTG 3 cut(s) 783, 822, 909
MvaI CCWGG 7 cut(s) 32, 209, 571, 620, 634, 829, 893
MvnI CGCG 5 cut(s) 74, 138, 179, 189, 219
MwoI GCNNNNNNNGC 8 cut(s) 43, 144, 225, 234, 279, 615, 818, 833
NciI CCSGG 2 cut(s) 279, 304
NcoI CCATGG 1 cut(s) 613
NdeII GATC 6 cut(s) 198, 261, 402, 489, 888, 918
NlaIII CATG 4 cut(s) 19, 205, 617, 904
NlaIV GGNNCC 5 cut(s) 92, 277, 325, 466, 638
NmeAIII GCCGAG 2 cut(s) 193, 217
NmuCI GTSAC 2 cut(s) 377, 738
NotI GCGGCCGC 1 cut(s) 184
PceI AGGCCT 1 cut(s) 332
PcsI WCGNNNNNNNCGW 2 cut(s) 71, 165
PfeI GAWTC 2 cut(s) 881, 989
Pfl23II CGTACG 1 cut(s) 66
PflFI GACNNNGTC 1 cut(s) 245
PflMI CCANNNNNTGG 2 cut(s) 474, 619
PfoI TCCNGGA 2 cut(s) 302, 891
PkrI GCNGC 7 cut(s) 123, 134, 137, 185, 188, 549, 838
Ple19I CGATCG 1 cut(s) 405
PleI GAGTC 3 cut(s) 339, 383, 592
PpsI GAGTC 3 cut(s) 339, 383, 592
Psp6I CCWGG 7 cut(s) 30, 207, 569, 618, 632, 827, 891
PspFI CCCAGC 3 cut(s) 223, 447, 483
PspGI CCWGG 7 cut(s) 30, 207, 569, 618, 632, 827, 891
PspLI CGTACG 1 cut(s) 66
PspN4I GGNNCC 5 cut(s) 92, 277, 325, 466, 638
PspOMI GGGCCC 1 cut(s) 275
PspPI GGNCC 7 cut(s) 205, 275, 276, 281, 295, 481, 533
PsuI RGATCY 2 cut(s) 261, 888
PsyI GACNNNGTC 1 cut(s) 245
PvuI CGATCG 1 cut(s) 405
PvuII CAGCTG 2 cut(s) 61, 97
RsaI GTAC 2 cut(s) 68, 359
RsaNI GTAC 2 cut(s) 67, 358
RseI CAYNNNNRTG 1 cut(s) 997
SalI GTCGAC 1 cut(s) 362
SaqAI TTAA 2 cut(s) 768, 929
SatI GCNGC 7 cut(s) 122, 133, 136, 184, 187, 548, 837
Sau3AI GATC 6 cut(s) 198, 261, 402, 489, 888, 918
Sau96I GGNCC 7 cut(s) 205, 275, 276, 281, 295, 481, 533
SchI GAGTC 3 cut(s) 339, 383, 593
ScrFI CCNGG 9 cut(s) 32, 209, 279, 304, 571, 620, 634, 829, 893
SduI GDGCHC 5 cut(s) 279, 326, 467, 641, 660
SinI GGWCC 1 cut(s) 533
SmiMI CAYNNNNRTG 1 cut(s) 997
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 5 cut(s) 427, 507, 783, 822, 909
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 5 cut(s) 37, 136, 183, 187, 273
SspMI CTAG 2 cut(s) 728, 809
StuI AGGCCT 1 cut(s) 332
StyD4I CCNGG 9 cut(s) 30, 207, 277, 302, 569, 618, 632, 827, 891
StyI CCWWGG 2 cut(s) 327, 613
TaaI ACNGT 3 cut(s) 377, 415, 940
TaiI ACGT 4 cut(s) 8, 105, 363, 756
TaqI TCGA 4 cut(s) 159, 348, 363, 405
TasI AATT 5 cut(s) 427, 507, 783, 822, 909
TauI GCSGC 3 cut(s) 138, 186, 189
TfiI GAWTC 2 cut(s) 881, 989
Tru1I TTAA 2 cut(s) 768, 929
Tru9I TTAA 2 cut(s) 768, 929
TscAI CASTG 3 cut(s) 134, 475, 747
TseFI GTSAC 2 cut(s) 377, 738
TseI GCWGC 4 cut(s) 121, 132, 547, 836
Tsp45I GTSAC 2 cut(s) 377, 738
TspDTI ATGAA 2 cut(s) 440, 917
TspRI CASTG 3 cut(s) 134, 475, 747
Tth111I GACNNNGTC 1 cut(s) 245
Van91I CCANNNNNTGG 2 cut(s) 474, 619
VpaK11BI GGWCC 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 2 cut(s) 220, 739
XmiI GTMKAC 2 cut(s) 174, 363
XspI CTAG 2 cut(s) 728, 809
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.