FvH4_4g06960

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018022
Physical Location & Seq
Forward (+)
6320665 .. 6327770
7106 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06960.t2

Sequence Viewer

Length: 1410 bp
ATGGAGAGGAACACCATCTCCCGTAAAGTCAACTCCTCCTCACCACCAAGCTTCTCCTCCTCCGCCGCGGAAACCGTAGCCGATATCCAAGACCTCTTAACAGAAATCCTTCTCCGTGTGCCAGCTCGATCAGTTGTTCGTCTCAAATGTATCTCCAAGCATTGGTACTGTCTCATCTCCAAACCCGAGTTCCGTCGCCGCCACATCCTCCAAAACCCCAACCCCAAAGTCTCTGCTTTCTTCTCCAACACCACTCAAGACTTCACCTTCATTTCTCTCTATAAAAACCCACCTCGTATTTTCATCCATGACCTTTACCCTGGTAACCATCGACACTCTATCAACCCCTTCAAAACTCTCAACATCTTTAATAACTTCGTTCAAGGCAACTGGAATGATTTTGGGTTGAAGATTCTTCAGTCCTGCAATGGCCTCTTTCTCTGCGTTCCATTCTCTACTCATAGAAGAACATATCAATATTATCACACCGTACAATATTATTCTATCTATGTTGTGAATCCCACCACCAACGAATTCATGGCTGTTTCACCTCCGAGGGTTGGGAAAGGAACTGTGTTCGAGCGATATGCTCTGGCTTTTGACCCTTTCAAATCAACTCATTGCAAGGTGATCTGCATTAGTACCATTCACAAGCACAATTGGCTGCACCAGGTGGACGTTTATTCGTCGGAGACTCGATCTTGGAGGCTTCTCAAGACGTCTTTCGCCTCCAGGGACATTGAGATGCAGTTTGATGGCAGGAGCAGGGAAGGAGGTGTGTACTGCAATGGCGCAGCTCATTGGATACGAGACGAGAAGGAGCTGCCGATTAGTGTTGGTGAGGATGGAAACAGTTTGGTAAGAGAGAATGCGGATTTGGTACATTACTATGACATATCTGAGGAACGTTTGGGACTTGCCATTCCTAGTGCTCCTGTTGTTGTAAGTGGATACTATCCTTCTAAGTTTCCGAAATTTGATCATAGGTATTTTGGGGAGTCTGGTGGTCATTTGTGTTTGATTGATGTATACAAGGACCACTCATCAGAAAAGTATTTATATAATGATTGTAATGCTGAATTTGATGTCATGGAGATGGGGAAGGACTACTCTGGATGGTTTGTCAAGTATCATGTTGATCTTAATCCGGTTGTTGCTGCATTCCGTGGCTATTATGATCACTTCTCTCGTTTTTATGTCTTGTTTCTTGATGGAGAGGGAAAAGAGGAACAAGTTCAGCAGCAGCCCGAGGAGGTTTCTTCATCTCTTTTGTTCCATATTCCTGGTAAGGTCATATCTTATAATCTTAGGAGTAAGACCTGTAAGACATATGTGGACTTGTCCATTAAGGACTATTTTCTAGTTGGGGGACATAGTTATAGTTACATGGAGACTTTAGCTAGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

470

Amino Acids

54.19

Weight (kDa)

7.95

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 31 - 66 3.3e-06 F-box domain
FBA_1 PF07734 133 - 305 4.1e-10 F-box associated beta propeller domain
FBA_3 PF08268 136 - 261 8.8e-07 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018022)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06960 FvH4_4g06960
rosa_chinensis RchiOBHm_Chr4g0399001
rosa_laevigata RLG00000009302
rosa_multiflora Rmu_sc0003033.1_g000018
rosa_roxburghii Rroxscaffold_5G00344060
rosa_rugosa Rorug04G0013600
rosa_samantha Rh4AG091400 Rh4BG089100 Rh4DG084200
rosa_wichuraiana Rw4G007570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1302
AatII GACGTC 1 cut(s) 722
AccB7I CCANNNNNTGG 1 cut(s) 162
AccI GTMKAC 1 cut(s) 1029
AccII CGCG 1 cut(s) 68
AciI CCGC 5 cut(s) 63, 66, 68, 199, 872
AclI AACGTT 1 cut(s) 907
AcsI RAATTY 3 cut(s) 533, 974, 1079
AcuI CTGAAG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 719
AdeI CACNNNGTG 1 cut(s) 673
AfaI GTAC 5 cut(s) 167, 492, 643, 782, 882
AfiI CCNNNNNNNGG 2 cut(s) 162, 560
AgsI TTSAA 4 cut(s) 352, 383, 409, 610
AjnI CCWGG 4 cut(s) 319, 669, 731, 1282
AjuI GAANNNNNNNTTGG 4 cut(s) 173, 205, 860, 892
AloI GAACNNNNNNTCC 1 cut(s) 34
AluBI AGCT 5 cut(s) 51, 125, 797, 823, 1400
AluI AGCT 5 cut(s) 51, 125, 797, 823, 1400
Alw21I GWGCWC 1 cut(s) 934
Alw26I GTCTC 6 cut(s) 146, 176, 235, 686, 804, 1385
Ama87I CYCGRG 2 cut(s) 185, 1247
AoxI GGCC 1 cut(s) 430
ApeKI GCWGC 6 cut(s) 664, 794, 823, 1157, 1240, 1243
ApoI RAATTY 3 cut(s) 533, 974, 1079
Asp700I GAANNNNTTC 2 cut(s) 108, 1233
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 1 cut(s) 1036
AsuHPI GGTGA 5 cut(s) 33, 256, 540, 640, 851
AvaI CYCGRG 2 cut(s) 185, 1247
AvaII GGWCC 1 cut(s) 1036
Bbv12I GWGCWC 1 cut(s) 934
BbvI GCAGC 6 cut(s) 651, 806, 810, 1144, 1252, 1255
BccI CCATC 7 cut(s) 23, 336, 749, 839, 1090, 1110, 1205
BcgI CGANNNNNNTGC 2 cut(s) 569, 603
BciT130I CCWGG 4 cut(s) 321, 671, 733, 1284
BciVI GTATCC 2 cut(s) 798, 944
BclI TGATCA 2 cut(s) 979, 1177
BcoDI GTCTC 6 cut(s) 146, 176, 235, 686, 804, 1385
BfaI CTAG 3 cut(s) 927, 1361, 1401
BfuI GTATCC 2 cut(s) 798, 944
BisI GCNGC 8 cut(s) 66, 199, 665, 795, 824, 1158, 1241, 1244
BlsI GCNGC 8 cut(s) 67, 200, 666, 796, 825, 1159, 1242, 1245
Bme1390I CCNGG 4 cut(s) 321, 671, 733, 1284
Bme18I GGWCC 1 cut(s) 1036
BmeT110I CYCGRG 2 cut(s) 185, 1247
BmgT120I GGNCC 1 cut(s) 1036
BmrFI CCNGG 4 cut(s) 321, 671, 733, 1284
BmsI GCATC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 715
BpuEI CTTGAG 2 cut(s) 240, 698
BsaBI GATNNNNATC 1 cut(s) 1143
BsaHI GRCGYC 1 cut(s) 719
BsaJI CCNNGG 6 cut(s) 66, 319, 554, 732, 1165, 1248
BsaWI WCCGGW 1 cut(s) 1147
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 560
Bse1I ACTGG 1 cut(s) 395
Bse3DI GCAATG 3 cut(s) 433, 619, 793
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 4 cut(s) 321, 671, 733, 1284
BseDI CCNNGG 6 cut(s) 66, 319, 554, 732, 1165, 1248
BseGI GGATG 4 cut(s) 204, 303, 850, 1121
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 2 cut(s) 162, 560
BseMI GCAATG 3 cut(s) 433, 619, 793
BseMII CTCAG 1 cut(s) 891
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 5 cut(s) 25, 28, 46, 49, 1265
BseXI GCAGC 6 cut(s) 651, 806, 810, 1144, 1252, 1255
BsgI GTGCAG 1 cut(s) 650
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 934
BsiHKCI CYCGRG 2 cut(s) 185, 1247
BsiSI CCGG 1 cut(s) 1148
BslFI GGGAC 3 cut(s) 749, 927, 1383
BslI CCNNNNNNNGG 2 cut(s) 162, 560
BsmAI GTCTC 6 cut(s) 146, 176, 235, 686, 804, 1385
BsmBI CGTCTC 2 cut(s) 146, 804
BsmFI GGGAC 3 cut(s) 749, 927, 1383
BsmI GAATGC 2 cut(s) 874, 1160
BsnI GGCC 1 cut(s) 432
BsoBI CYCGRG 2 cut(s) 185, 1247
Bsp1286I GDGCHC 1 cut(s) 934
Bsp143I GATC 6 cut(s) 128, 630, 698, 979, 1138, 1177
BspACI CCGC 5 cut(s) 63, 66, 68, 199, 872
BspANI GGCC 1 cut(s) 432
BspCNI CTCAG 1 cut(s) 892
BspFNI CGCG 1 cut(s) 68
BsrDI GCAATG 3 cut(s) 433, 619, 793
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 6 cut(s) 66, 319, 554, 732, 1165, 1248
BssMI GATC 6 cut(s) 128, 630, 698, 979, 1138, 1177
BssNAI GTATAC 1 cut(s) 1030
BssNI GRCGYC 1 cut(s) 719
Bst1107I GTATAC 1 cut(s) 1030
Bst2UI CCWGG 4 cut(s) 321, 671, 733, 1284
Bst4CI ACNGT 5 cut(s) 76, 170, 490, 574, 854
BstACI GRCGYC 1 cut(s) 719
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 3 cut(s) 900, 963, 1307
BstDSI CCRYGG 2 cut(s) 66, 1165
BstEII GGTNACC 1 cut(s) 323
BstF5I GGATG 4 cut(s) 204, 303, 850, 1121
BstFNI CGCG 1 cut(s) 68
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 6 cut(s) 131, 633, 701, 982, 1141, 1180
BstMAI GTCTC 6 cut(s) 146, 176, 235, 686, 804, 1385
BstMBI GATC 6 cut(s) 128, 630, 698, 979, 1138, 1177
BstMWI GCNNNNNNNGC 1 cut(s) 661
BstNI CCWGG 4 cut(s) 321, 671, 733, 1284
BstPI GGTNACC 1 cut(s) 323
BstSCI CCNGG 4 cut(s) 319, 669, 731, 1282
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 6 cut(s) 651, 806, 810, 1144, 1252, 1255
BstXI CCANNNNNNTGG 1 cut(s) 1283
BstZ17I GTATAC 1 cut(s) 1030
BsuI GTATCC 2 cut(s) 798, 944
BsuRI GGCC 1 cut(s) 432
BtgI CCRYGG 2 cut(s) 66, 1165
BtsCI GGATG 4 cut(s) 204, 303, 850, 1121
Cac8I GCNNGC 1 cut(s) 123
CfoI GCGC 1 cut(s) 794
Cfr13I GGNCC 1 cut(s) 1036
Cfr42I CCGCGG 1 cut(s) 69
CsiI ACCWGGT 1 cut(s) 669
Csp6I GTAC 5 cut(s) 166, 491, 642, 781, 881
CviAII CATG 5 cut(s) 308, 538, 1090, 1133, 1387
CviQI GTAC 5 cut(s) 166, 491, 642, 781, 881
DdeI CTNAG 3 cut(s) 900, 963, 1307
DpnI GATC 6 cut(s) 130, 632, 700, 981, 1140, 1179
DpnII GATC 6 cut(s) 128, 630, 698, 979, 1138, 1177
DraIII CACNNNGTG 1 cut(s) 673
EciI GGCGGA 1 cut(s) 52
Eco32I GATATC 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 1036
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 2 cut(s) 185, 1247
Eco91I GGTNACC 1 cut(s) 323
EcoO65I GGTNACC 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 533
EcoRII CCWGG 4 cut(s) 319, 669, 731, 1282
EcoRV GATATC 1 cut(s) 85
Esp3I CGTCTC 2 cut(s) 146, 804
FaeI CATG 5 cut(s) 311, 541, 1093, 1136, 1390
FaqI GGGAC 3 cut(s) 749, 927, 1383
FatI CATG 5 cut(s) 307, 537, 1089, 1132, 1386
FauNDI CATATG 1 cut(s) 1330
FbaI TGATCA 2 cut(s) 979, 1177
FblI GTMKAC 1 cut(s) 1029
Fnu4HI GCNGC 8 cut(s) 66, 199, 665, 795, 824, 1158, 1241, 1244
FokI GGATG 4 cut(s) 191, 290, 857, 1128
Fsp4HI GCNGC 8 cut(s) 66, 199, 665, 795, 824, 1158, 1241, 1244
FspBI CTAG 3 cut(s) 927, 1361, 1401
GlaI GCGC 1 cut(s) 793
GluI GCNGC 8 cut(s) 66, 199, 665, 795, 824, 1158, 1241, 1244
GsuI CTGGAG 1 cut(s) 715
HaeIII GGCC 1 cut(s) 432
HapII CCGG 1 cut(s) 1148
HhaI GCGC 1 cut(s) 794
Hin1I GRCGYC 1 cut(s) 719
Hin1II CATG 5 cut(s) 311, 541, 1093, 1136, 1390
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 49
HinfI GANTC 4 cut(s) 412, 517, 694, 998
HpaII CCGG 1 cut(s) 1148
HphI GGTGA 5 cut(s) 33, 256, 540, 640, 851
Hpy166II GTNNAC 5 cut(s) 31, 676, 781, 1030, 1336
Hpy188I TCNGA 6 cut(s) 555, 691, 901, 972, 1048, 1409
Hpy188III TCNNGA 4 cut(s) 257, 715, 1113, 1208
Hpy8I GTNNAC 5 cut(s) 31, 676, 781, 1030, 1336
Hpy99I CGWCG 2 cut(s) 198, 691
HpyAV CCTTC 7 cut(s) 119, 277, 358, 764, 811, 969, 1096
HpyCH4III ACNGT 5 cut(s) 76, 170, 490, 574, 854
HpyCH4IV ACGT 3 cut(s) 678, 719, 907
HpyCH4V TGCA 7 cut(s) 426, 624, 636, 667, 748, 786, 1160
HpyF10VI GCNNNNNNNGC 1 cut(s) 661
HpyF3I CTNAG 3 cut(s) 900, 963, 1307
HpySE526I ACGT 3 cut(s) 678, 719, 907
Hsp92I GRCGYC 1 cut(s) 719
Hsp92II CATG 5 cut(s) 311, 541, 1093, 1136, 1390
HspAI GCGC 1 cut(s) 792
Ksp22I TGATCA 2 cut(s) 979, 1177
KspI CCGCGG 1 cut(s) 69
Kzo9I GATC 6 cut(s) 128, 630, 698, 979, 1138, 1177
LmnI GCTCC 3 cut(s) 762, 820, 937
Lsp1109I GCAGC 6 cut(s) 651, 806, 810, 1144, 1252, 1255
LweI GCATC 1 cut(s) 735
MabI ACCWGGT 1 cut(s) 669
MaeI CTAG 3 cut(s) 927, 1361, 1401
MaeII ACGT 3 cut(s) 678, 719, 907
MaeIII GTNAC 2 cut(s) 323, 1382
MalI GATC 6 cut(s) 130, 632, 700, 981, 1140, 1179
MboI GATC 6 cut(s) 128, 630, 698, 979, 1138, 1177
MboII GAAGA 5 cut(s) 232, 407, 421, 477, 1251
MfeI CAATTG 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 934
MluCI AATT 4 cut(s) 533, 658, 974, 1079
MlyI GAGTC 2 cut(s) 688, 1007
MmeI TCCRAC 2 cut(s) 270, 669
MroXI GAANNNNTTC 2 cut(s) 108, 1233
MseI TTAA 4 cut(s) 98, 369, 1143, 1347
MslI CAYNNNNRTG 3 cut(s) 743, 888, 1094
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 1 cut(s) 1148
MspR9I CCNGG 4 cut(s) 321, 671, 733, 1284
MunI CAATTG 1 cut(s) 658
Mva1269I GAATGC 2 cut(s) 874, 1160
MvaI CCWGG 4 cut(s) 321, 671, 733, 1284
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 661
NdeI CATATG 1 cut(s) 1330
NdeII GATC 6 cut(s) 128, 630, 698, 979, 1138, 1177
NlaIII CATG 5 cut(s) 311, 541, 1093, 1136, 1390
PctI GAATGC 2 cut(s) 874, 1160
PdmI GAANNNNTTC 2 cut(s) 108, 1233
PfeI GAWTC 2 cut(s) 412, 517
PflMI CCANNNNNTGG 1 cut(s) 162
PkrI GCNGC 8 cut(s) 67, 200, 666, 796, 825, 1159, 1242, 1245
PleI GAGTC 2 cut(s) 688, 1006
PpsI GAGTC 2 cut(s) 688, 1006
PsiI TTATAA 1 cut(s) 1302
Psp1406I AACGTT 1 cut(s) 907
Psp6I CCWGG 4 cut(s) 319, 669, 731, 1282
PspEI GGTNACC 1 cut(s) 323
PspGI CCWGG 4 cut(s) 319, 669, 731, 1282
PspPI GGNCC 1 cut(s) 1036
RsaI GTAC 5 cut(s) 167, 492, 643, 782, 882
RsaNI GTAC 5 cut(s) 166, 491, 642, 781, 881
RseI CAYNNNNRTG 3 cut(s) 743, 888, 1094
SacII CCGCGG 1 cut(s) 69
SaqAI TTAA 4 cut(s) 98, 369, 1143, 1347
SatI GCNGC 8 cut(s) 66, 199, 665, 795, 824, 1158, 1241, 1244
Sau3AI GATC 6 cut(s) 128, 630, 698, 979, 1138, 1177
Sau96I GGNCC 1 cut(s) 1036
SchI GAGTC 2 cut(s) 688, 1007
ScrFI CCNGG 4 cut(s) 321, 671, 733, 1284
SduI GDGCHC 1 cut(s) 934
SexAI ACCWGGT 1 cut(s) 669
SfaNI GCATC 1 cut(s) 735
Sfr303I CCGCGG 1 cut(s) 69
SgrBI CCGCGG 1 cut(s) 69
SinI GGWCC 1 cut(s) 1036
SmiMI CAYNNNNRTG 3 cut(s) 743, 888, 1094
SmlI CTYRAG 2 cut(s) 255, 713
SmoI CTYRAG 2 cut(s) 255, 713
Sse9I AATT 4 cut(s) 533, 658, 974, 1079
SsiI CCGC 5 cut(s) 63, 66, 68, 199, 872
SspI AATATT 2 cut(s) 479, 497
SspMI CTAG 3 cut(s) 927, 1361, 1401
StyD4I CCNGG 4 cut(s) 319, 669, 731, 1282
TaaI ACNGT 5 cut(s) 76, 170, 490, 574, 854
TaiI ACGT 3 cut(s) 681, 722, 910
TaqI TCGA 4 cut(s) 127, 331, 579, 697
TasI AATT 4 cut(s) 533, 658, 974, 1079
TatI WGTACW 1 cut(s) 780
TauI GCSGC 2 cut(s) 68, 201
TfiI GAWTC 2 cut(s) 412, 517
Tru1I TTAA 4 cut(s) 98, 369, 1143, 1347
Tru9I TTAA 4 cut(s) 98, 369, 1143, 1347
TseI GCWGC 6 cut(s) 664, 794, 823, 1157, 1240, 1243
TspDTI ATGAA 4 cut(s) 259, 292, 526, 1251
TspGWI ACGGA 3 cut(s) 104, 182, 1154
Van91I CCANNNNNTGG 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 1036
XapI RAATTY 3 cut(s) 533, 974, 1079
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 1029
XmnI GAANNNNTTC 2 cut(s) 108, 1233
XspI CTAG 3 cut(s) 927, 1361, 1401
ZraI GACGTC 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.