Rh4BG089100

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0018022
Physical Location & Seq
Forward (+)
15379064 .. 15380035
972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG089100.1

Sequence Viewer

Length: 972 bp
ATGGAGAGGAAGACTATCTCCCGTAGAGTCAACTCATCACCACTGAGCTTCTCCTCCTCCGCCGCGGAAACCGTCGCCGACATCGAAGACCTTCTAATAGAAATCCTTCTCTGTGTGCCAGCTCGATCAGTTGTTCGTCTCAAATGCATCTCCAAGCATTGGTTCTCTCTCATCTCAAACCCCGAGTTCCGTCACCGCCACACCCTCCGAAACCCGAACCCCAAAGTCTCTGCTTTCTTCTCCCACACAACCCAAGACTTCACCTCCATTTCTCTCCATAAAAACTCATCTGGGTCTTTCCCACCTCGTCTTTTTGTCCATGACCTTCACACTGTTAACCATCAACGCTCTATCAACCCCTTCAAAAGTCTCAACATCGTTAATAGTTTCGTCCAAGGCGATCGGAATGGGTTTGGGTTGAAGATTCTTCAGTCCTGCAATGGCCTCTTTCTGTGCATTCCATTTTCTACACACAAAAGAAGACATTATCATTCCATCTATTTTGGCAATCCCACATCACAAGATCATTCGATCTATGTTGTCAATCCCACCTCCAACCAATTCTTGCCTCTTTCGCCTCCGAGGGCTGGGAAGAGCACAGAATTCGTGCGGTATGCTCTGGCTTTTGACCCTTCCAAATCGCCTCATTACAAGGTGGTCTGCCTTAGTACTACCCGCAAGCACAATTGGGTGCACCAGGTGGATGTTTATTCGTCTGAGACTGGATCTTGGAGGATTCTCGAGACGCCTTACGCCTCCAGGGACCTTGAGATGCACTTGGATGGCAGGAGCAGGGAAGGAGGTGTGTACTGCAACGGCGCGATTCATTGGATAAGAGACGAAACAGAGCTGCCGATTCGTGATGGTGGGGATGGAAACAGTTTGGTAAGAGAGAACGGTGATTTGGTACATTACTATGACATTGCTGAAGAACGTTTGGACTTGCCATTCCTAGTGCCCCTCTGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

323

Amino Acids

36.88

Weight (kDa)

8.86

Isoelectric Point (pI)

57.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 29 - 65 6.6e-06 F-box domain
FBA_1 PF07734 138 - 313 3e-11 F-box associated beta propeller domain
FBA_3 PF08268 159 - 270 3.9e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018022)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06960 FvH4_4g06960
rosa_chinensis RchiOBHm_Chr4g0399001
rosa_laevigata RLG00000009302
rosa_multiflora Rmu_sc0003033.1_g000018
rosa_roxburghii Rroxscaffold_5G00344060
rosa_rugosa Rorug04G0013600
rosa_samantha Rh4AG091400 Rh4BG089100 Rh4DG084200
rosa_wichuraiana Rw4G007570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 159
AccII CGCG 2 cut(s) 65, 821
AciI CCGC 6 cut(s) 60, 63, 65, 196, 610, 676
AclI AACGTT 1 cut(s) 934
AclWI GGATC 1 cut(s) 733
AcsI RAATTY 1 cut(s) 602
AcuI CTGAAG 2 cut(s) 413, 948
AcyI GRCGYC 1 cut(s) 746
AdeI CACNNNGTG 1 cut(s) 700
AfaI GTAC 3 cut(s) 670, 809, 909
AfiI CCNNNNNNNGG 2 cut(s) 159, 587
AgsI TTSAA 2 cut(s) 364, 421
AjnI CCWGG 2 cut(s) 696, 758
AjuI GAANNNNNNNTTGG 4 cut(s) 146, 178, 887, 919
AluBI AGCT 3 cut(s) 48, 122, 850
AluI AGCT 3 cut(s) 48, 122, 850
Alw21I GWGCWC 2 cut(s) 599, 696
Alw26I GTCTC 6 cut(s) 143, 232, 374, 713, 737, 831
Alw44I GTGCAC 1 cut(s) 692
AlwI GGATC 1 cut(s) 733
Ama87I CYCGRG 2 cut(s) 182, 740
AoxI GGCC 1 cut(s) 442
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 1 cut(s) 850
ApoI RAATTY 1 cut(s) 602
Asp700I GAANNNNTTC 2 cut(s) 90, 105
AspLEI GCGC 1 cut(s) 821
AspS9I GGNCC 1 cut(s) 763
AsuHPI GGTGA 4 cut(s) 30, 185, 253, 911
AvaI CYCGRG 2 cut(s) 182, 740
AvaII GGWCC 1 cut(s) 763
BaeGI GKGCMC 2 cut(s) 696, 960
BbsI GAAGAC 3 cut(s) 17, 93, 487
Bbv12I GWGCWC 2 cut(s) 599, 696
BbvI GCAGC 1 cut(s) 837
BccI CCATC 5 cut(s) 348, 503, 776, 857, 866
BceAI ACGGC 1 cut(s) 832
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 2 cut(s) 698, 760
BcoDI GTCTC 6 cut(s) 143, 232, 374, 713, 737, 831
BfaI CTAG 1 cut(s) 953
BisI GCNGC 2 cut(s) 63, 851
BlsI GCNGC 2 cut(s) 64, 852
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 2 cut(s) 698, 760
Bme18I GGWCC 1 cut(s) 763
BmeT110I CYCGRG 2 cut(s) 182, 740
BmgT120I GGNCC 1 cut(s) 763
BmiI GGNNCC 1 cut(s) 764
BmrFI CCNGG 2 cut(s) 698, 760
BmsI GCATC 2 cut(s) 156, 762
BpiI GAAGAC 3 cut(s) 17, 93, 487
BpmI CTGGAG 1 cut(s) 742
BpuEI CTTGAG 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 746
BsaJI CCNNGG 4 cut(s) 63, 394, 581, 759
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 587
Bse1I ACTGG 1 cut(s) 727
Bse3DI GCAATG 2 cut(s) 445, 921
BseBI CCWGG 2 cut(s) 698, 760
BseDI CCNNGG 4 cut(s) 63, 394, 581, 759
BseGI GGATG 3 cut(s) 709, 787, 877
BseLI CCNNNNNNNGG 2 cut(s) 159, 587
BseMI GCAATG 2 cut(s) 445, 921
BseMII CTCAG 2 cut(s) 35, 708
BseNI ACTGG 1 cut(s) 727
BseRI GAGGAG 2 cut(s) 43, 46
BseSI GKGCMC 2 cut(s) 696, 960
BseXI GCAGC 1 cut(s) 837
BseYI CCCAGC 1 cut(s) 587
Bsh1236I CGCG 2 cut(s) 65, 821
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 1 cut(s) 444
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 2 cut(s) 599, 696
BsiHKCI CYCGRG 2 cut(s) 182, 740
BslFI GGGAC 1 cut(s) 776
BslI CCNNNNNNNGG 2 cut(s) 159, 587
BsmAI GTCTC 6 cut(s) 143, 232, 374, 713, 737, 831
BsmBI CGTCTC 3 cut(s) 143, 737, 831
BsmFI GGGAC 1 cut(s) 776
BsmI GAATGC 1 cut(s) 456
BsnI GGCC 1 cut(s) 444
BsoBI CYCGRG 2 cut(s) 182, 740
Bsp1286I GDGCHC 3 cut(s) 599, 696, 960
Bsp143I GATC 5 cut(s) 125, 400, 523, 531, 725
BspACI CCGC 6 cut(s) 60, 63, 65, 196, 610, 676
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 36, 709
BspFNI CGCG 2 cut(s) 65, 821
BspLI GGNNCC 1 cut(s) 764
BspPI GGATC 1 cut(s) 733
BspQI GCTCTTC 1 cut(s) 587
BsrDI GCAATG 2 cut(s) 445, 921
BsrI ACTGG 1 cut(s) 727
BssECI CCNNGG 4 cut(s) 63, 394, 581, 759
BssMI GATC 5 cut(s) 125, 400, 523, 531, 725
BssNI GRCGYC 1 cut(s) 746
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 2 cut(s) 698, 760
Bst4CI ACNGT 4 cut(s) 73, 334, 881, 899
Bst6I CTCTTC 1 cut(s) 587
BstACI GRCGYC 1 cut(s) 746
BstC8I GCNNGC 2 cut(s) 120, 680
BstDEI CTNAG 3 cut(s) 44, 665, 717
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 3 cut(s) 709, 787, 877
BstFNI CGCG 2 cut(s) 65, 821
BstHHI GCGC 1 cut(s) 821
BstKTI GATC 5 cut(s) 128, 403, 526, 534, 728
BstMAI GTCTC 6 cut(s) 143, 232, 374, 713, 737, 831
BstMBI GATC 5 cut(s) 125, 400, 523, 531, 725
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 1 cut(s) 574
BstNI CCWGG 2 cut(s) 698, 760
BstSCI CCNGG 2 cut(s) 696, 758
BstSLI GKGCMC 2 cut(s) 696, 960
BstUI CGCG 2 cut(s) 65, 821
BstV1I GCAGC 1 cut(s) 837
BstV2I GAAGAC 3 cut(s) 17, 93, 487
BstX2I RGATCY 1 cut(s) 725
BstYI RGATCY 1 cut(s) 725
BsuRI GGCC 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 3 cut(s) 709, 787, 877
BtsIMutI CAGTG 2 cut(s) 41, 330
Cac8I GCNNGC 2 cut(s) 120, 680
CfoI GCGC 1 cut(s) 821
Cfr13I GGNCC 1 cut(s) 763
Cfr42I CCGCGG 1 cut(s) 66
CseI GACGC 1 cut(s) 754
CsiI ACCWGGT 1 cut(s) 696
Csp6I GTAC 3 cut(s) 669, 808, 908
CviAII CATG 1 cut(s) 320
CviJI RGCY 6 cut(s) 48, 122, 444, 587, 623, 850
CviKI_1 RGCY 6 cut(s) 48, 122, 444, 587, 623, 850
CviQI GTAC 3 cut(s) 669, 808, 908
DdeI CTNAG 3 cut(s) 44, 665, 717
DpnI GATC 5 cut(s) 127, 402, 525, 533, 727
DpnII GATC 5 cut(s) 125, 400, 523, 531, 725
DraIII CACNNNGTG 1 cut(s) 700
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
EciI GGCGGA 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 763
Eco57I CTGAAG 2 cut(s) 413, 948
Eco88I CYCGRG 2 cut(s) 182, 740
EcoO109I RGGNCCY 1 cut(s) 763
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 2 cut(s) 696, 758
EcoT14I CCWWGG 1 cut(s) 394
EcoT22I ATGCAT 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 394
Esp3I CGTCTC 3 cut(s) 143, 737, 831
FaeI CATG 1 cut(s) 323
FaiI YATR 5 cut(s) 279, 321, 537, 615, 918
FaqI GGGAC 1 cut(s) 776
FatI CATG 1 cut(s) 319
FauI CCCGC 1 cut(s) 683
Fnu4HI GCNGC 2 cut(s) 63, 851
FokI GGATG 3 cut(s) 716, 794, 884
Fsp4HI GCNGC 2 cut(s) 63, 851
FspBI CTAG 1 cut(s) 953
GlaI GCGC 1 cut(s) 820
GluI GCNGC 2 cut(s) 63, 851
GsaI CCCAGC 1 cut(s) 591
GsuI CTGGAG 1 cut(s) 742
HaeIII GGCC 1 cut(s) 444
HgaI GACGC 1 cut(s) 754
HhaI GCGC 1 cut(s) 821
Hin1I GRCGYC 1 cut(s) 746
Hin1II CATG 1 cut(s) 323
Hin6I GCGC 1 cut(s) 819
HinP1I GCGC 1 cut(s) 819
HincII GTYRAC 2 cut(s) 31, 337
HindII GTYRAC 2 cut(s) 31, 337
HinfI GANTC 5 cut(s) 27, 424, 736, 823, 856
HpaI GTTAAC 1 cut(s) 337
HphI GGTGA 4 cut(s) 30, 185, 253, 911
Hpy166II GTNNAC 4 cut(s) 31, 337, 694, 808
Hpy188I TCNGA 4 cut(s) 209, 405, 582, 718
Hpy188III TCNNGA 3 cut(s) 740, 742, 860
Hpy8I GTNNAC 4 cut(s) 31, 337, 694, 808
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 6 cut(s) 101, 116, 335, 370, 642, 791
HpyCH4III ACNGT 4 cut(s) 73, 334, 881, 899
HpyCH4IV ACGT 1 cut(s) 934
HpyCH4V TGCA 6 cut(s) 147, 438, 456, 694, 775, 813
HpyF10VI GCNNNNNNNGC 1 cut(s) 574
HpyF3I CTNAG 3 cut(s) 44, 665, 717
HpySE526I ACGT 1 cut(s) 934
Hsp92I GRCGYC 1 cut(s) 746
Hsp92II CATG 1 cut(s) 323
HspAI GCGC 1 cut(s) 819
KspAI GTTAAC 1 cut(s) 337
KspI CCGCGG 1 cut(s) 66
Kzo9I GATC 5 cut(s) 125, 400, 523, 531, 725
LguI GCTCTTC 1 cut(s) 587
LmnI GCTCC 1 cut(s) 789
Lsp1109I GCAGC 1 cut(s) 837
LweI GCATC 2 cut(s) 156, 762
MabI ACCWGGT 1 cut(s) 696
MaeI CTAG 1 cut(s) 953
MaeII ACGT 1 cut(s) 934
MaeIII GTNAC 1 cut(s) 191
MalI GATC 5 cut(s) 127, 402, 525, 533, 727
MboI GATC 5 cut(s) 125, 400, 523, 531, 725
MboII GAAGA 8 cut(s) 22, 98, 229, 419, 433, 492, 604, 941
MfeI CAATTG 1 cut(s) 685
MflI RGATCY 1 cut(s) 725
MhlI GDGCHC 3 cut(s) 599, 696, 960
MluCI AATT 3 cut(s) 560, 602, 685
MlyI GAGTC 1 cut(s) 36
MmeI TCCRAC 1 cut(s) 579
Mph1103I ATGCAT 1 cut(s) 149
MroXI GAANNNNTTC 2 cut(s) 90, 105
MseI TTAA 2 cut(s) 336, 381
MslI CAYNNNNRTG 2 cut(s) 780, 915
MspA1I CMGCKG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 698, 760
MunI CAATTG 1 cut(s) 685
Mva1269I GAATGC 1 cut(s) 456
MvaI CCWGG 2 cut(s) 698, 760
MvnI CGCG 2 cut(s) 65, 821
MwoI GCNNNNNNNGC 1 cut(s) 574
NdeII GATC 5 cut(s) 125, 400, 523, 531, 725
NlaIII CATG 1 cut(s) 323
NlaIV GGNNCC 1 cut(s) 764
NmuCI GTSAC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 740
PciSI GCTCTTC 1 cut(s) 587
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 396
PctI GAATGC 1 cut(s) 456
PdmI GAANNNNTTC 2 cut(s) 90, 105
PfeI GAWTC 4 cut(s) 424, 736, 823, 856
PflMI CCANNNNNTGG 1 cut(s) 159
PkrI GCNGC 2 cut(s) 64, 852
Ple19I CGATCG 1 cut(s) 403
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PpuMI RGGWCCY 1 cut(s) 763
Psp1406I AACGTT 1 cut(s) 934
Psp5II RGGWCCY 1 cut(s) 763
Psp6I CCWGG 2 cut(s) 696, 758
PspFI CCCAGC 1 cut(s) 587
PspGI CCWGG 2 cut(s) 696, 758
PspN4I GGNNCC 1 cut(s) 764
PspPI GGNCC 1 cut(s) 763
PspPPI RGGWCCY 1 cut(s) 763
PsuI RGATCY 1 cut(s) 725
PvuI CGATCG 1 cut(s) 403
RsaI GTAC 3 cut(s) 670, 809, 909
RsaNI GTAC 3 cut(s) 669, 808, 908
RseI CAYNNNNRTG 2 cut(s) 780, 915
SacII CCGCGG 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 587
SaqAI TTAA 2 cut(s) 336, 381
SatI GCNGC 2 cut(s) 63, 851
Sau3AI GATC 5 cut(s) 125, 400, 523, 531, 725
Sau96I GGNCC 1 cut(s) 763
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 1 cut(s) 36
ScrFI CCNGG 2 cut(s) 698, 760
SduI GDGCHC 3 cut(s) 599, 696, 960
SexAI ACCWGGT 1 cut(s) 696
SfaNI GCATC 2 cut(s) 156, 762
Sfr274I CTCGAG 1 cut(s) 740
Sfr303I CCGCGG 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 66
SinI GGWCC 1 cut(s) 763
SlaI CTCGAG 1 cut(s) 740
SmiMI CAYNNNNRTG 2 cut(s) 780, 915
SmlI CTYRAG 2 cut(s) 740, 767
SmoI CTYRAG 2 cut(s) 740, 767
Sse9I AATT 3 cut(s) 560, 602, 685
SsiI CCGC 6 cut(s) 60, 63, 65, 196, 610, 676
SspMI CTAG 1 cut(s) 953
StyD4I CCNGG 2 cut(s) 696, 758
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 4 cut(s) 73, 334, 881, 899
TaiI ACGT 1 cut(s) 937
TaqI TCGA 4 cut(s) 84, 124, 530, 741
TasI AATT 3 cut(s) 560, 602, 685
TatI WGTACW 2 cut(s) 668, 807
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 4 cut(s) 424, 736, 823, 856
Tru1I TTAA 2 cut(s) 336, 381
Tru9I TTAA 2 cut(s) 336, 381
TscAI CASTG 2 cut(s) 48, 337
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 1 cut(s) 850
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 815
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 2 cut(s) 48, 337
Van91I CCANNNNNTGG 1 cut(s) 159
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 1 cut(s) 763
XapI RAATTY 1 cut(s) 602
XhoI CTCGAG 1 cut(s) 740
XmnI GAANNNNTTC 2 cut(s) 90, 105
XspI CTAG 1 cut(s) 953
ZrmI AGTACT 1 cut(s) 670
Zsp2I ATGCAT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.