Rh4DG084200

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018022
Physical Location & Seq
Forward (+)
12781713 .. 12783798
2086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG084200.1

Sequence Viewer

Length: 1437 bp
ATGGAGAGGAAGACTATCTCCCGTAGAGTCAACTCATCACCACTGAGCTTCTCCTCCTCCGCCGCGGAAACCGTCGCCGACATCGAAGAACTTCTAACAGAAATCCTTCTCTGTGTGCCAGCTCGATCAGTCGTTCGTCTCAAATGCATCTCCAAGCATTGGTTCTCTCTAATCTCAAACCCCGAGTTCCGTCACCGCCACACCCTCCAAAACCCGAACCCCAAAGTCTCTGCTTTCTTCTCCCACACAACCCAAGACTTCACCTTCCTTTCTCTCCATAAAAACCCATCTGGGTCTTTCCCACGTCGTCTTTTCGTCCATGACCTTCACACTGCTAACCATCAACGCTCTATCAACCCCTTCAAAAGTCTCAACATCTTTAATAGTTTCGTCCAAGGCGATCGGAATGGGTTTGGGTTGAAGATTCTTCAGTCCTGCAATGGCCTCTTTCTGTGCATTCCATTTTCTACACACAAAAGAAGACATTATCATTCCATCTATTTTGGCAATCCCACATCACAATATCATTCTATCTATGTTGTCAATCCCACCTCCAACCAATTCTTGGCTCTTTCGCCTCCGAGGGCTGGGAAGAGCACAGAGTTCGTGCGGTATGCTCTGGCTTTTGACCCTTCCAAATCGCCTCATTACAAGGTGGTCTGCCTTAGTACTACCCGCAAGCACAATTGGGTGCACCAGGTGGACGTTTATTCGTCTGAGACTCGATCTTGGAGGCTTCTCGAGACGCCTTACGCCTCCAGGGACCTTGATATGCACTTGGATGGCAGGAGCAGGGAAGGAGCTGTGTACTGCAACGGCGCGATTCATTGGATAAGAGACGAAACAGAGCTGCCGATTCGTGTTGGTGAGGATGGAAACAGTTTGGTAAGAGAGAACGCTGATTTGGTACATTACTATGACATAGCTGAAGAACGTTTGGGACTTGCCATTCCTAGTGCCCCTCTGGTTGTAATTGGATACTATCCTCCTAAGTTTCCGAAATTTGATCATAGATATTTTGGGGAGTCTGGTGATCATTTGTATTTGATTGATGTATATGAGGACTCTTCGTCAAAAAATAATATATATAAGGATTGTAATGCTGAATTTGATGTCATGGAGATGGGCAAGGACTACTCTGGCTGGTTTGTCAAGTATCATGTTGATCTTAATCCCGTAGTTGCTGCATTCCGTGGCAATTATGATTACTTTTCTCGTTTTTATGTCTTGTTTCTTGGTGGAGAGGGAAAAGAAGAACAAGATCAACAGCAGCAGGAGGAGGTTTCTTCATCTCTTTTGTTCCATGTTCCTGGTAAGGTCATATCTTATCATCTTAGACATAAGACCTTTAAGACATATGTGGACTTGGCTATTAAGGACTATTTTCTAGTTGGGGGATATAATTATCCTTACATGGAGACTTTAGCTTCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

478

Amino Acids

55.08

Weight (kDa)

7.72

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 143 - 313 1.9e-10 F-box associated beta propeller domain
FBA_3 PF08268 166 - 271 2.5e-09 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018022)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06960 FvH4_4g06960
rosa_chinensis RchiOBHm_Chr4g0399001
rosa_laevigata RLG00000009302
rosa_multiflora Rmu_sc0003033.1_g000018
rosa_roxburghii Rroxscaffold_5G00344060
rosa_rugosa Rorug04G0013600
rosa_samantha Rh4AG091400 Rh4BG089100 Rh4DG084200
rosa_wichuraiana Rw4G007570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 159, 565
AccII CGCG 2 cut(s) 65, 821
AciI CCGC 6 cut(s) 60, 63, 65, 196, 610, 676
AclI AACGTT 1 cut(s) 934
AcsI RAATTY 2 cut(s) 1001, 1106
AcuI CTGAAG 2 cut(s) 413, 948
AcyI GRCGYC 1 cut(s) 746
AdeI CACNNNGTG 1 cut(s) 700
AfaI GTAC 3 cut(s) 670, 809, 909
AfiI CCNNNNNNNGG 3 cut(s) 159, 565, 587
AgsI TTSAA 2 cut(s) 364, 421
AhdI GACNNNNNGTC 1 cut(s) 1069
AjiI CACGTC 1 cut(s) 305
AjnI CCWGG 3 cut(s) 696, 758, 1309
AjuI GAANNNNNNNTTGG 4 cut(s) 146, 178, 887, 919
AluBI AGCT 6 cut(s) 48, 122, 803, 850, 926, 1427
AluI AGCT 6 cut(s) 48, 122, 803, 850, 926, 1427
Alw21I GWGCWC 2 cut(s) 599, 696
Alw26I GTCTC 7 cut(s) 143, 232, 374, 713, 737, 831, 1412
Alw44I GTGCAC 1 cut(s) 692
Ama87I CYCGRG 2 cut(s) 182, 740
AoxI GGCC 1 cut(s) 442
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 3 cut(s) 850, 1184, 1270
ApoI RAATTY 2 cut(s) 1001, 1106
Asp700I GAANNNNTTC 2 cut(s) 90, 105
AspLEI GCGC 1 cut(s) 821
AspS9I GGNCC 1 cut(s) 763
AsuHPI GGTGA 5 cut(s) 30, 185, 253, 878, 1043
AvaI CYCGRG 2 cut(s) 182, 740
AvaII GGWCC 1 cut(s) 763
BaeGI GKGCMC 2 cut(s) 696, 961
BbsI GAAGAC 2 cut(s) 17, 487
Bbv12I GWGCWC 2 cut(s) 599, 696
BbvI GCAGC 3 cut(s) 837, 1171, 1282
BccI CCATC 6 cut(s) 295, 348, 503, 776, 866, 1117
BceAI ACGGC 1 cut(s) 832
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 3 cut(s) 698, 760, 1311
BciVI GTATCC 1 cut(s) 971
BclI TGATCA 2 cut(s) 1006, 1033
BcoDI GTCTC 7 cut(s) 143, 232, 374, 713, 737, 831, 1412
BfaI CTAG 2 cut(s) 954, 1388
BfuI GTATCC 1 cut(s) 971
BisI GCNGC 4 cut(s) 63, 851, 1185, 1271
BlsI GCNGC 4 cut(s) 64, 852, 1186, 1272
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 3 cut(s) 698, 760, 1311
Bme18I GGWCC 1 cut(s) 763
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmeT110I CYCGRG 2 cut(s) 182, 740
BmgBI CACGTC 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 763
BmiI GGNNCC 1 cut(s) 764
BmrFI CCNGG 3 cut(s) 698, 760, 1311
BmsI GCATC 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 17, 487
BpmI CTGGAG 1 cut(s) 742
BsaBI GATNNNNATC 1 cut(s) 1170
BsaHI GRCGYC 1 cut(s) 746
BsaJI CCNNGG 5 cut(s) 63, 394, 581, 759, 1192
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 565, 587
Bse3DI GCAATG 1 cut(s) 445
Bse8I GATNNNNATC 1 cut(s) 1170
BseBI CCWGG 3 cut(s) 698, 760, 1311
BseDI CCNNGG 5 cut(s) 63, 394, 581, 759, 1192
BseGI GGATG 2 cut(s) 787, 877
BseJI GATNNNNATC 1 cut(s) 1170
BseLI CCNNNNNNNGG 3 cut(s) 159, 565, 587
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 2 cut(s) 35, 708
BseRI GAGGAG 3 cut(s) 43, 46, 1292
BseSI GKGCMC 2 cut(s) 696, 961
BseXI GCAGC 3 cut(s) 837, 1171, 1282
BseYI CCCAGC 1 cut(s) 587
Bsh1236I CGCG 2 cut(s) 65, 821
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 1 cut(s) 444
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 2 cut(s) 599, 696
BsiHKCI CYCGRG 2 cut(s) 182, 740
BslFI GGGAC 2 cut(s) 776, 954
BslI CCNNNNNNNGG 3 cut(s) 159, 565, 587
BsmAI GTCTC 7 cut(s) 143, 232, 374, 713, 737, 831, 1412
BsmBI CGTCTC 3 cut(s) 143, 737, 831
BsmFI GGGAC 2 cut(s) 776, 954
BsmI GAATGC 2 cut(s) 456, 1187
BsnI GGCC 1 cut(s) 444
BsoBI CYCGRG 2 cut(s) 182, 740
Bsp1286I GDGCHC 3 cut(s) 599, 696, 961
Bsp143I GATC 7 cut(s) 125, 400, 725, 1006, 1033, 1165, 1261
BspACI CCGC 6 cut(s) 60, 63, 65, 196, 610, 676
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 36, 709
BspFNI CGCG 2 cut(s) 65, 821
BspLI GGNNCC 1 cut(s) 764
BspQI GCTCTTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 445
BssECI CCNNGG 5 cut(s) 63, 394, 581, 759, 1192
BssMI GATC 7 cut(s) 125, 400, 725, 1006, 1033, 1165, 1261
BssNI GRCGYC 1 cut(s) 746
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 3 cut(s) 698, 760, 1311
Bst4CI ACNGT 2 cut(s) 73, 881
Bst6I CTCTTC 2 cut(s) 587, 1072
BstACI GRCGYC 1 cut(s) 746
BstC8I GCNNGC 2 cut(s) 120, 680
BstDEI CTNAG 5 cut(s) 44, 665, 717, 990, 1334
BstDSI CCRYGG 2 cut(s) 63, 1192
BstF5I GGATG 2 cut(s) 787, 877
BstFNI CGCG 2 cut(s) 65, 821
BstHHI GCGC 1 cut(s) 821
BstKTI GATC 7 cut(s) 128, 403, 728, 1009, 1036, 1168, 1264
BstMAI GTCTC 7 cut(s) 143, 232, 374, 713, 737, 831, 1412
BstMBI GATC 7 cut(s) 125, 400, 725, 1006, 1033, 1165, 1261
BstMCI CGRYCG 1 cut(s) 403
BstNI CCWGG 3 cut(s) 698, 760, 1311
BstSCI CCNGG 3 cut(s) 696, 758, 1309
BstSLI GKGCMC 2 cut(s) 696, 961
BstUI CGCG 2 cut(s) 65, 821
BstV1I GCAGC 3 cut(s) 837, 1171, 1282
BstV2I GAAGAC 2 cut(s) 17, 487
BstXI CCANNNNNNTGG 1 cut(s) 1310
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 1 cut(s) 444
BtgI CCRYGG 2 cut(s) 63, 1192
BtrI CACGTC 1 cut(s) 305
BtsCI GGATG 2 cut(s) 787, 877
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 2 cut(s) 41, 330
Cac8I GCNNGC 2 cut(s) 120, 680
CfoI GCGC 1 cut(s) 821
Cfr13I GGNCC 1 cut(s) 763
Cfr42I CCGCGG 1 cut(s) 66
CseI GACGC 1 cut(s) 754
CsiI ACCWGGT 1 cut(s) 696
Csp6I GTAC 3 cut(s) 669, 808, 908
CviAII CATG 5 cut(s) 320, 1117, 1160, 1304, 1414
CviQI GTAC 3 cut(s) 669, 808, 908
DdeI CTNAG 5 cut(s) 44, 665, 717, 990, 1334
DpnI GATC 7 cut(s) 127, 402, 727, 1008, 1035, 1167, 1263
DpnII GATC 7 cut(s) 125, 400, 725, 1006, 1033, 1165, 1261
DraIII CACNNNGTG 1 cut(s) 700
DriI GACNNNNNGTC 1 cut(s) 1069
Eam1104I CTCTTC 2 cut(s) 587, 1072
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EarI CTCTTC 2 cut(s) 587, 1072
EciI GGCGGA 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 763
Eco57I CTGAAG 2 cut(s) 413, 948
Eco88I CYCGRG 2 cut(s) 182, 740
EcoO109I RGGNCCY 1 cut(s) 763
EcoRII CCWGG 3 cut(s) 696, 758, 1309
EcoT14I CCWWGG 1 cut(s) 394
EcoT22I ATGCAT 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 394
Esp3I CGTCTC 3 cut(s) 143, 737, 831
FaeI CATG 5 cut(s) 323, 1120, 1163, 1307, 1417
FaqI GGGAC 2 cut(s) 776, 954
FatI CATG 5 cut(s) 319, 1116, 1159, 1303, 1413
FauI CCCGC 1 cut(s) 683
FauNDI CATATG 1 cut(s) 1357
FbaI TGATCA 2 cut(s) 1006, 1033
Fnu4HI GCNGC 4 cut(s) 63, 851, 1185, 1271
FokI GGATG 2 cut(s) 794, 884
Fsp4HI GCNGC 4 cut(s) 63, 851, 1185, 1271
FspBI CTAG 2 cut(s) 954, 1388
GlaI GCGC 1 cut(s) 820
GluI GCNGC 4 cut(s) 63, 851, 1185, 1271
GsaI CCCAGC 1 cut(s) 591
GsuI CTGGAG 1 cut(s) 742
HaeIII GGCC 1 cut(s) 444
HgaI GACGC 1 cut(s) 754
HhaI GCGC 1 cut(s) 821
Hin1I GRCGYC 1 cut(s) 746
Hin1II CATG 5 cut(s) 323, 1120, 1163, 1307, 1417
Hin6I GCGC 1 cut(s) 819
HinP1I GCGC 1 cut(s) 819
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 7 cut(s) 27, 424, 721, 823, 856, 1025, 1064
HphI GGTGA 5 cut(s) 30, 185, 253, 878, 1043
Hpy166II GTNNAC 5 cut(s) 31, 694, 703, 808, 1363
Hpy188I TCNGA 4 cut(s) 405, 582, 718, 999
Hpy188III TCNNGA 2 cut(s) 740, 742
Hpy8I GTNNAC 5 cut(s) 31, 694, 703, 808, 1363
Hpy99I CGWCG 2 cut(s) 77, 309
HpyAV CCTTC 6 cut(s) 116, 274, 335, 370, 642, 791
HpyCH4III ACNGT 2 cut(s) 73, 881
HpyCH4IV ACGT 3 cut(s) 304, 705, 934
HpyCH4V TGCA 7 cut(s) 147, 438, 456, 694, 775, 813, 1187
HpyF3I CTNAG 5 cut(s) 44, 665, 717, 990, 1334
HpySE526I ACGT 3 cut(s) 304, 705, 934
Hsp92I GRCGYC 1 cut(s) 746
Hsp92II CATG 5 cut(s) 323, 1120, 1163, 1307, 1417
HspAI GCGC 1 cut(s) 819
Ksp22I TGATCA 2 cut(s) 1006, 1033
KspI CCGCGG 1 cut(s) 66
Kzo9I GATC 7 cut(s) 125, 400, 725, 1006, 1033, 1165, 1261
LguI GCTCTTC 1 cut(s) 587
LmnI GCTCC 2 cut(s) 789, 800
Lsp1109I GCAGC 3 cut(s) 837, 1171, 1282
LweI GCATC 1 cut(s) 156
MabI ACCWGGT 1 cut(s) 696
MaeI CTAG 2 cut(s) 954, 1388
MaeII ACGT 3 cut(s) 304, 705, 934
MaeIII GTNAC 1 cut(s) 191
MalI GATC 7 cut(s) 127, 402, 727, 1008, 1035, 1167, 1263
MboI GATC 7 cut(s) 125, 400, 725, 1006, 1033, 1165, 1261
MfeI CAATTG 1 cut(s) 685
MhlI GDGCHC 3 cut(s) 599, 696, 961
MluCI AATT 7 cut(s) 560, 685, 972, 1001, 1106, 1198, 1402
MlyI GAGTC 4 cut(s) 36, 715, 1034, 1058
MmeI TCCRAC 1 cut(s) 579
Mph1103I ATGCAT 1 cut(s) 149
MroXI GAANNNNTTC 2 cut(s) 90, 105
MseI TTAA 4 cut(s) 381, 1170, 1350, 1374
MslI CAYNNNNRTG 3 cut(s) 780, 915, 1121
MspA1I CMGCKG 1 cut(s) 65
MspR9I CCNGG 3 cut(s) 698, 760, 1311
MunI CAATTG 1 cut(s) 685
Mva1269I GAATGC 2 cut(s) 456, 1187
MvaI CCWGG 3 cut(s) 698, 760, 1311
MvnI CGCG 2 cut(s) 65, 821
NdeI CATATG 1 cut(s) 1357
NdeII GATC 7 cut(s) 125, 400, 725, 1006, 1033, 1165, 1261
NlaIII CATG 5 cut(s) 323, 1120, 1163, 1307, 1417
NlaIV GGNNCC 1 cut(s) 764
NmuCI GTSAC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 740
PciSI GCTCTTC 1 cut(s) 587
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 396
PctI GAATGC 2 cut(s) 456, 1187
PdmI GAANNNNTTC 2 cut(s) 90, 105
PfeI GAWTC 3 cut(s) 424, 823, 856
PflMI CCANNNNNTGG 2 cut(s) 159, 565
PkrI GCNGC 4 cut(s) 64, 852, 1186, 1272
Ple19I CGATCG 1 cut(s) 403
PleI GAGTC 4 cut(s) 35, 715, 1033, 1058
PpsI GAGTC 4 cut(s) 35, 715, 1033, 1058
PpuMI RGGWCCY 1 cut(s) 763
Psp1406I AACGTT 1 cut(s) 934
Psp5II RGGWCCY 1 cut(s) 763
Psp6I CCWGG 3 cut(s) 696, 758, 1309
PspFI CCCAGC 1 cut(s) 587
PspGI CCWGG 3 cut(s) 696, 758, 1309
PspN4I GGNNCC 1 cut(s) 764
PspPI GGNCC 1 cut(s) 763
PspPPI RGGWCCY 1 cut(s) 763
PvuI CGATCG 1 cut(s) 403
RsaI GTAC 3 cut(s) 670, 809, 909
RsaNI GTAC 3 cut(s) 669, 808, 908
RseI CAYNNNNRTG 3 cut(s) 780, 915, 1121
SacII CCGCGG 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 587
SaqAI TTAA 4 cut(s) 381, 1170, 1350, 1374
SatI GCNGC 4 cut(s) 63, 851, 1185, 1271
Sau3AI GATC 7 cut(s) 125, 400, 725, 1006, 1033, 1165, 1261
Sau96I GGNCC 1 cut(s) 763
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 4 cut(s) 36, 715, 1034, 1058
ScrFI CCNGG 3 cut(s) 698, 760, 1311
SduI GDGCHC 3 cut(s) 599, 696, 961
SexAI ACCWGGT 1 cut(s) 696
SfaNI GCATC 1 cut(s) 156
Sfr274I CTCGAG 1 cut(s) 740
Sfr303I CCGCGG 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 66
SinI GGWCC 1 cut(s) 763
SlaI CTCGAG 1 cut(s) 740
SmiMI CAYNNNNRTG 3 cut(s) 780, 915, 1121
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 7 cut(s) 560, 685, 972, 1001, 1106, 1198, 1402
SsiI CCGC 6 cut(s) 60, 63, 65, 196, 610, 676
SspMI CTAG 2 cut(s) 954, 1388
StyD4I CCNGG 3 cut(s) 696, 758, 1309
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 2 cut(s) 73, 881
TaiI ACGT 3 cut(s) 307, 708, 937
TaqI TCGA 4 cut(s) 84, 124, 724, 741
TasI AATT 7 cut(s) 560, 685, 972, 1001, 1106, 1198, 1402
TatI WGTACW 2 cut(s) 668, 807
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 3 cut(s) 424, 823, 856
Tru1I TTAA 4 cut(s) 381, 1170, 1350, 1374
Tru9I TTAA 4 cut(s) 381, 1170, 1350, 1374
TscAI CASTG 2 cut(s) 48, 337
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 3 cut(s) 850, 1184, 1270
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 2 cut(s) 815, 1278
TspGWI ACGGA 2 cut(s) 179, 1181
TspRI CASTG 2 cut(s) 48, 337
Van91I CCANNNNNTGG 2 cut(s) 159, 565
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 1 cut(s) 763
XapI RAATTY 2 cut(s) 1001, 1106
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XhoI CTCGAG 1 cut(s) 740
XmnI GAANNNNTTC 2 cut(s) 90, 105
XspI CTAG 2 cut(s) 954, 1388
ZrmI AGTACT 1 cut(s) 670
Zsp2I ATGCAT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.