Rroxscaffold_5G00344060

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0018022
Physical Location & Seq
Forward (+)
13988160 .. 13992488
4329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00344060.1

Sequence Viewer

Length: 1467 bp
ATGGAGAGGAAGACTATCTCCCGTAGAGTCAACTCATCACCACTGAGCTTCTCCTCCTCCGCCGCGGAAACCGTCGCCGACATCGAAGAACTTCTAACAGAAATCCTTCTCTGTGTGCCAGCTCGATCAGTCGTTCGTCTCAAATGCATCTCCAAGCATTGGTTCTCTCTCATCTCAAACCCCGAGTTCGGTCACCGCCACACCCTCCGAAACCCGAACCCCAAAGTCTCTGCTTTCTTCTCCCACACAACCCAAGACTTCACCTTCATTTCTCTCCATAAAAACCCATCTGGGTCTTTCCCACCTCGTCATTTCATCCATGACCTTCACCCTGGTAACCATCAACGCTCTATCAACCCCTTCAAAAGTCTCAACATCTTTAATAGTTTCGTCCAAGGCGATCGGAATGGGTTTGGGCTGAAGATTCTTCAGTCCTGCAATGGCCTCTTTCTGTGCATTCCATTTTCTACACACAAAAGAAGACATCATCATTCCATCTATTTTGGCAATCCCACATCACAATATCATTCTATCTATGTTGTCAATCCCACCTCCAACCAATTCTTGGCTCTTTCGCCTCCGAGGGCTGGGAAAAGCACAGAATTCGTGCGATATGCTCTGGCTTTTGACCCTTCCAAATCGCCTCATTACAAGGTGGTCTGCCTTAGTACTACCCGCAAGCACAATTGGGTGCACCAGGTGGACGTTTATTCGTCTGAGACTCGATCTTGGAGGCTTCTCGAGACGCCTTACGCCTCCAGGGACCTTGAGATGCACTTGGATGGCAGGAGCAGGGAAGGAGCTGTGTACTGCAACGGCGCGATTCATTGGATAAGAGACGAAACAGAGCTGCCGATTCGTGTTGGTGGGGATGGAAACAGTTTGGTAAGAGAGAACGCTGATTTGGTACATTACTATGACATTGCTGAAGAACGTTTGGGACTTGCCATTCCTAGTGCCCCTCTGGTTGTAATTGGAAACTATCCTCCTAAGTTTCCGAAATTTGATCATAGATATTTTGGGGAGTCTGGTGATCATTTGTATTTGATTGATGTATATAAGGACTCTTCGTCGAAAAATAATATATATAAGGATTGTAGTGCTGAATTTGATGTCATGGAGATGGGCAAGGGCTACTCTGGCTGGTTTGTCAAGTATCATGTTGATCTTAATCCCGTAGTTGCTGCATTCCGTGGCAATTATGATCACTTTTCTCGTTTTTATGTCTTGTTTCTTGGTGGAGAGGGAAAAGAAGAACAAGATCAACAGCAACAGGAGGAGGTTTCTTCATCTCTTTTGTTCCATGTTCCTGGTGGCACATTGAAATTAATTCTCAAGGAATTAATCTACAAGAGGGGCTATGGAAAGCTCAACAAGCAGAGAACTGCTTTGACCGACAATTCTGTTGTTGAACAGGCTTTGGGTAAGCATGGAATCATTTGCGTGGAGGACCTTATATATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

488

Amino Acids

55.6

Weight (kDa)

8.6

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 143 - 313 3.1e-10 F-box associated beta propeller domain
FBA_3 PF08268 166 - 271 1.7e-09 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018022)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06960 FvH4_4g06960
rosa_chinensis RchiOBHm_Chr4g0399001
rosa_laevigata RLG00000009302
rosa_multiflora Rmu_sc0003033.1_g000018
rosa_roxburghii Rroxscaffold_5G00344060
rosa_rugosa Rorug04G0013600
rosa_samantha Rh4AG091400 Rh4BG089100 Rh4DG084200
rosa_wichuraiana Rw4G007570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 159, 565
AccII CGCG 2 cut(s) 65, 821
AciI CCGC 5 cut(s) 60, 63, 65, 196, 676
AclI AACGTT 1 cut(s) 934
AcsI RAATTY 3 cut(s) 602, 1001, 1106
AcuI CTGAAG 3 cut(s) 413, 440, 948
AcyI GRCGYC 1 cut(s) 746
AdeI CACNNNGTG 1 cut(s) 700
AfaI GTAC 3 cut(s) 670, 809, 909
AfiI CCNNNNNNNGG 4 cut(s) 159, 188, 565, 587
AgsI TTSAA 3 cut(s) 364, 1324, 1412
AhdI GACNNNNNGTC 1 cut(s) 1069
AjnI CCWGG 4 cut(s) 331, 696, 758, 1309
AjuI GAANNNNNNNTTGG 4 cut(s) 146, 178, 887, 919
AluBI AGCT 5 cut(s) 48, 122, 803, 850, 1369
AluI AGCT 5 cut(s) 48, 122, 803, 850, 1369
Alw21I GWGCWC 1 cut(s) 696
Alw26I GTCTC 6 cut(s) 143, 232, 374, 713, 737, 831
Alw44I GTGCAC 1 cut(s) 692
Ama87I CYCGRG 2 cut(s) 182, 740
AoxI GGCC 1 cut(s) 442
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 2 cut(s) 850, 1184
ApoI RAATTY 3 cut(s) 602, 1001, 1106
AseI ATTAAT 2 cut(s) 1328, 1343
Asp700I GAANNNNTTC 2 cut(s) 90, 105
AspLEI GCGC 1 cut(s) 821
AspS9I GGNCC 2 cut(s) 763, 1450
AsuHPI GGTGA 5 cut(s) 30, 185, 253, 320, 1043
AvaI CYCGRG 2 cut(s) 182, 740
AvaII GGWCC 2 cut(s) 763, 1450
BaeGI GKGCMC 2 cut(s) 696, 961
BbsI GAAGAC 2 cut(s) 17, 487
Bbv12I GWGCWC 1 cut(s) 696
BbvI GCAGC 2 cut(s) 837, 1171
BccI CCATC 6 cut(s) 295, 348, 503, 776, 866, 1117
BceAI ACGGC 1 cut(s) 832
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 4 cut(s) 333, 698, 760, 1311
BclI TGATCA 3 cut(s) 1006, 1033, 1204
BcoDI GTCTC 6 cut(s) 143, 232, 374, 713, 737, 831
BfaI CTAG 1 cut(s) 954
BisI GCNGC 3 cut(s) 63, 851, 1185
BlsI GCNGC 3 cut(s) 64, 852, 1186
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 4 cut(s) 333, 698, 760, 1311
Bme18I GGWCC 2 cut(s) 763, 1450
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmeT110I CYCGRG 2 cut(s) 182, 740
BmgT120I GGNCC 2 cut(s) 763, 1450
BmiI GGNNCC 1 cut(s) 764
BmrFI CCNGG 4 cut(s) 333, 698, 760, 1311
BmsI GCATC 2 cut(s) 156, 762
BpiI GAAGAC 2 cut(s) 17, 487
BpmI CTGGAG 1 cut(s) 742
BpuEI CTTGAG 2 cut(s) 788, 1319
BsaBI GATNNNNATC 1 cut(s) 1170
BsaHI GRCGYC 1 cut(s) 746
BsaJI CCNNGG 6 cut(s) 63, 331, 394, 581, 759, 1192
Bsc4I CCNNNNNNNGG 4 cut(s) 159, 188, 565, 587
Bse3DI GCAATG 2 cut(s) 445, 921
Bse8I GATNNNNATC 1 cut(s) 1170
BseBI CCWGG 4 cut(s) 333, 698, 760, 1311
BseDI CCNNGG 6 cut(s) 63, 331, 394, 581, 759, 1192
BseGI GGATG 3 cut(s) 315, 787, 877
BseJI GATNNNNATC 1 cut(s) 1170
BseLI CCNNNNNNNGG 4 cut(s) 159, 188, 565, 587
BseMI GCAATG 2 cut(s) 445, 921
BseMII CTCAG 2 cut(s) 35, 708
BseRI GAGGAG 3 cut(s) 43, 46, 1292
BseSI GKGCMC 2 cut(s) 696, 961
BseXI GCAGC 2 cut(s) 837, 1171
BseYI CCCAGC 1 cut(s) 587
Bsh1236I CGCG 2 cut(s) 65, 821
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 1 cut(s) 444
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 696
BsiHKCI CYCGRG 2 cut(s) 182, 740
BslFI GGGAC 2 cut(s) 776, 954
BslI CCNNNNNNNGG 4 cut(s) 159, 188, 565, 587
BsmAI GTCTC 6 cut(s) 143, 232, 374, 713, 737, 831
BsmBI CGTCTC 3 cut(s) 143, 737, 831
BsmFI GGGAC 2 cut(s) 776, 954
BsmI GAATGC 2 cut(s) 456, 1187
BsnI GGCC 1 cut(s) 444
BsoBI CYCGRG 2 cut(s) 182, 740
Bsp1286I GDGCHC 2 cut(s) 696, 961
Bsp143I GATC 8 cut(s) 125, 400, 725, 1006, 1033, 1165, 1204, 1261
BspACI CCGC 5 cut(s) 60, 63, 65, 196, 676
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 36, 709
BspFNI CGCG 2 cut(s) 65, 821
BspLI GGNNCC 1 cut(s) 764
BsrDI GCAATG 2 cut(s) 445, 921
BssECI CCNNGG 6 cut(s) 63, 331, 394, 581, 759, 1192
BssMI GATC 8 cut(s) 125, 400, 725, 1006, 1033, 1165, 1204, 1261
BssNI GRCGYC 1 cut(s) 746
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 4 cut(s) 333, 698, 760, 1311
Bst4CI ACNGT 2 cut(s) 73, 881
Bst6I CTCTTC 1 cut(s) 1072
BstACI GRCGYC 1 cut(s) 746
BstC8I GCNNGC 2 cut(s) 120, 680
BstDEI CTNAG 4 cut(s) 44, 665, 717, 990
BstDSI CCRYGG 2 cut(s) 63, 1192
BstEII GGTNACC 2 cut(s) 191, 335
BstF5I GGATG 3 cut(s) 315, 787, 877
BstFNI CGCG 2 cut(s) 65, 821
BstHHI GCGC 1 cut(s) 821
BstKTI GATC 8 cut(s) 128, 403, 728, 1009, 1036, 1168, 1207, 1264
BstMAI GTCTC 6 cut(s) 143, 232, 374, 713, 737, 831
BstMBI GATC 8 cut(s) 125, 400, 725, 1006, 1033, 1165, 1204, 1261
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 2 cut(s) 1140, 1375
BstNI CCWGG 4 cut(s) 333, 698, 760, 1311
BstPI GGTNACC 2 cut(s) 191, 335
BstSCI CCNGG 4 cut(s) 331, 696, 758, 1309
BstSLI GKGCMC 2 cut(s) 696, 961
BstUI CGCG 2 cut(s) 65, 821
BstV1I GCAGC 2 cut(s) 837, 1171
BstV2I GAAGAC 2 cut(s) 17, 487
BstXI CCANNNNNNTGG 1 cut(s) 1310
BsuRI GGCC 1 cut(s) 444
BtgI CCRYGG 2 cut(s) 63, 1192
BtsCI GGATG 3 cut(s) 315, 787, 877
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 2 cut(s) 120, 680
CfoI GCGC 1 cut(s) 821
Cfr13I GGNCC 2 cut(s) 763, 1450
Cfr42I CCGCGG 1 cut(s) 66
CseI GACGC 1 cut(s) 754
CsiI ACCWGGT 1 cut(s) 696
Csp6I GTAC 3 cut(s) 669, 808, 908
CviAII CATG 6 cut(s) 320, 1117, 1160, 1304, 1430, 1464
CviQI GTAC 3 cut(s) 669, 808, 908
DdeI CTNAG 4 cut(s) 44, 665, 717, 990
DpnI GATC 8 cut(s) 127, 402, 727, 1008, 1035, 1167, 1206, 1263
DpnII GATC 8 cut(s) 125, 400, 725, 1006, 1033, 1165, 1204, 1261
DraIII CACNNNGTG 1 cut(s) 700
DriI GACNNNNNGTC 1 cut(s) 1069
Eam1104I CTCTTC 1 cut(s) 1072
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EarI CTCTTC 1 cut(s) 1072
EciI GGCGGA 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 394
Eco47I GGWCC 2 cut(s) 763, 1450
Eco57I CTGAAG 3 cut(s) 413, 440, 948
Eco88I CYCGRG 2 cut(s) 182, 740
Eco91I GGTNACC 2 cut(s) 191, 335
EcoO109I RGGNCCY 2 cut(s) 763, 1450
EcoO65I GGTNACC 2 cut(s) 191, 335
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 4 cut(s) 331, 696, 758, 1309
EcoT14I CCWWGG 1 cut(s) 394
EcoT22I ATGCAT 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 394
Esp3I CGTCTC 3 cut(s) 143, 737, 831
FaeI CATG 6 cut(s) 323, 1120, 1163, 1307, 1433, 1467
FaqI GGGAC 2 cut(s) 776, 954
FatI CATG 6 cut(s) 319, 1116, 1159, 1303, 1429, 1463
FauI CCCGC 1 cut(s) 683
FbaI TGATCA 3 cut(s) 1006, 1033, 1204
Fnu4HI GCNGC 3 cut(s) 63, 851, 1185
FokI GGATG 3 cut(s) 302, 794, 884
Fsp4HI GCNGC 3 cut(s) 63, 851, 1185
FspBI CTAG 1 cut(s) 954
GlaI GCGC 1 cut(s) 820
GluI GCNGC 3 cut(s) 63, 851, 1185
GsaI CCCAGC 1 cut(s) 591
GsuI CTGGAG 1 cut(s) 742
HaeIII GGCC 1 cut(s) 444
HgaI GACGC 1 cut(s) 754
HhaI GCGC 1 cut(s) 821
Hin1I GRCGYC 1 cut(s) 746
Hin1II CATG 6 cut(s) 323, 1120, 1163, 1307, 1433, 1467
Hin6I GCGC 1 cut(s) 819
HinP1I GCGC 1 cut(s) 819
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 8 cut(s) 27, 424, 721, 823, 856, 1025, 1064, 1434
HphI GGTGA 5 cut(s) 30, 185, 253, 320, 1043
Hpy166II GTNNAC 4 cut(s) 31, 694, 703, 808
Hpy188I TCNGA 5 cut(s) 209, 405, 582, 718, 999
Hpy188III TCNNGA 2 cut(s) 740, 742
Hpy8I GTNNAC 4 cut(s) 31, 694, 703, 808
Hpy99I CGWCG 2 cut(s) 77, 1075
HpyAV CCTTC 6 cut(s) 116, 274, 335, 370, 642, 791
HpyCH4III ACNGT 2 cut(s) 73, 881
HpyCH4IV ACGT 2 cut(s) 705, 934
HpyCH4V TGCA 7 cut(s) 147, 438, 456, 694, 775, 813, 1187
HpyF10VI GCNNNNNNNGC 2 cut(s) 1140, 1375
HpyF3I CTNAG 4 cut(s) 44, 665, 717, 990
HpySE526I ACGT 2 cut(s) 705, 934
Hsp92I GRCGYC 1 cut(s) 746
Hsp92II CATG 6 cut(s) 323, 1120, 1163, 1307, 1433, 1467
HspAI GCGC 1 cut(s) 819
Ksp22I TGATCA 3 cut(s) 1006, 1033, 1204
KspI CCGCGG 1 cut(s) 66
Kzo9I GATC 8 cut(s) 125, 400, 725, 1006, 1033, 1165, 1204, 1261
LmnI GCTCC 2 cut(s) 789, 800
Lsp1109I GCAGC 2 cut(s) 837, 1171
LweI GCATC 2 cut(s) 156, 762
MabI ACCWGGT 1 cut(s) 696
MaeI CTAG 1 cut(s) 954
MaeII ACGT 2 cut(s) 705, 934
MaeIII GTNAC 2 cut(s) 191, 335
MalI GATC 8 cut(s) 127, 402, 727, 1008, 1035, 1167, 1206, 1263
MboI GATC 8 cut(s) 125, 400, 725, 1006, 1033, 1165, 1204, 1261
MfeI CAATTG 1 cut(s) 685
MhlI GDGCHC 2 cut(s) 696, 961
MlyI GAGTC 4 cut(s) 36, 715, 1034, 1058
MmeI TCCRAC 1 cut(s) 579
Mph1103I ATGCAT 1 cut(s) 149
MroXI GAANNNNTTC 2 cut(s) 90, 105
MseI TTAA 4 cut(s) 381, 1170, 1328, 1343
MslI CAYNNNNRTG 4 cut(s) 780, 915, 1121, 1442
MspA1I CMGCKG 1 cut(s) 65
MspR9I CCNGG 4 cut(s) 333, 698, 760, 1311
MunI CAATTG 1 cut(s) 685
Mva1269I GAATGC 2 cut(s) 456, 1187
MvaI CCWGG 4 cut(s) 333, 698, 760, 1311
MvnI CGCG 2 cut(s) 65, 821
MwoI GCNNNNNNNGC 2 cut(s) 1140, 1375
NdeII GATC 8 cut(s) 125, 400, 725, 1006, 1033, 1165, 1204, 1261
NlaIII CATG 6 cut(s) 323, 1120, 1163, 1307, 1433, 1467
NlaIV GGNNCC 1 cut(s) 764
NmuCI GTSAC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 740
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 396
PctI GAATGC 2 cut(s) 456, 1187
PdmI GAANNNNTTC 2 cut(s) 90, 105
PfeI GAWTC 4 cut(s) 424, 823, 856, 1434
PflMI CCANNNNNTGG 2 cut(s) 159, 565
PkrI GCNGC 3 cut(s) 64, 852, 1186
Ple19I CGATCG 1 cut(s) 403
PleI GAGTC 4 cut(s) 35, 715, 1033, 1058
PpsI GAGTC 4 cut(s) 35, 715, 1033, 1058
PpuMI RGGWCCY 2 cut(s) 763, 1450
PshBI ATTAAT 2 cut(s) 1328, 1343
Psp1406I AACGTT 1 cut(s) 934
Psp5II RGGWCCY 2 cut(s) 763, 1450
Psp6I CCWGG 4 cut(s) 331, 696, 758, 1309
PspEI GGTNACC 2 cut(s) 191, 335
PspFI CCCAGC 1 cut(s) 587
PspGI CCWGG 4 cut(s) 331, 696, 758, 1309
PspN4I GGNNCC 1 cut(s) 764
PspPI GGNCC 2 cut(s) 763, 1450
PspPPI RGGWCCY 2 cut(s) 763, 1450
PvuI CGATCG 1 cut(s) 403
RsaI GTAC 3 cut(s) 670, 809, 909
RsaNI GTAC 3 cut(s) 669, 808, 908
RseI CAYNNNNRTG 4 cut(s) 780, 915, 1121, 1442
SacII CCGCGG 1 cut(s) 66
SaqAI TTAA 4 cut(s) 381, 1170, 1328, 1343
SatI GCNGC 3 cut(s) 63, 851, 1185
Sau3AI GATC 8 cut(s) 125, 400, 725, 1006, 1033, 1165, 1204, 1261
Sau96I GGNCC 2 cut(s) 763, 1450
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 4 cut(s) 36, 715, 1034, 1058
ScrFI CCNGG 4 cut(s) 333, 698, 760, 1311
SduI GDGCHC 2 cut(s) 696, 961
SexAI ACCWGGT 1 cut(s) 696
SfaNI GCATC 2 cut(s) 156, 762
Sfr274I CTCGAG 1 cut(s) 740
Sfr303I CCGCGG 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 66
SinI GGWCC 2 cut(s) 763, 1450
SlaI CTCGAG 1 cut(s) 740
SmiMI CAYNNNNRTG 4 cut(s) 780, 915, 1121, 1442
SmlI CTYRAG 3 cut(s) 740, 767, 1334
SmoI CTYRAG 3 cut(s) 740, 767, 1334
SsiI CCGC 5 cut(s) 60, 63, 65, 196, 676
SspMI CTAG 1 cut(s) 954
StyD4I CCNGG 4 cut(s) 331, 696, 758, 1309
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 2 cut(s) 73, 881
TaiI ACGT 2 cut(s) 708, 937
TaqI TCGA 5 cut(s) 84, 124, 724, 741, 1073
TaqII GACCGA 2 cut(s) 179, 1409
TatI WGTACW 2 cut(s) 668, 807
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 4 cut(s) 424, 823, 856, 1434
Tru1I TTAA 4 cut(s) 381, 1170, 1328, 1343
Tru9I TTAA 4 cut(s) 381, 1170, 1328, 1343
TscAI CASTG 1 cut(s) 48
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 2 cut(s) 850, 1184
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 4 cut(s) 256, 304, 815, 1278
TspGWI ACGGA 1 cut(s) 1181
TspRI CASTG 1 cut(s) 48
Van91I CCANNNNNTGG 2 cut(s) 159, 565
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 2 cut(s) 763, 1450
VspI ATTAAT 2 cut(s) 1328, 1343
XapI RAATTY 3 cut(s) 602, 1001, 1106
XcmI CCANNNNNNNNNTGG 2 cut(s) 562, 1310
XhoI CTCGAG 1 cut(s) 740
XmnI GAANNNNTTC 2 cut(s) 90, 105
XspI CTAG 1 cut(s) 954
ZrmI AGTACT 1 cut(s) 670
Zsp2I ATGCAT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.