Rw4G007570

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018022
Physical Location & Seq
Forward (+)
16273660 .. 16278437
4778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007570.1

Sequence Viewer

Length: 2184 bp
ATGGAGAGGAAGACCATCTCCCGTAGAGTCAACTCATCACCACTGAGCTTCTCCTCCTCCGCCGCGGAAACCGTCGCCGACATCGAAGAACTTCTAACAGAAATCCTTCTGTGTGTGCCAGCTCGATCAGTTGGTCGTCTCAAATGCATCTCCAAGCATTGGTTCTCTCTAATCTCAAACCCCGAGTTCCGTCACCGCCACACCCTCCGAAACCCGAACCCCAAAGTCTCTGCTTTCTTCTCCCACACAACCCAAGACTTCACCTTCATTTCTCTCCATAAAAACCCATCTGGGTCTTTCCCACCTCGTCATTTCGTCCATGACCTTCACCCTGGTAACCATGAACGCTCTATCAACCCCTTCAAAAGTCTCAACATCTTTAATAGTTTCGTCCAAGGCGATCGGAATGGGTTTGGGTTGAAGATTCTTCAGTCCTGCAATGGCCTCTTTCTCTGCATTCCATTTTCTACACACAAAAGCAGACATTATCATTCCATCTATTTTGGCAATCCCACATCACAATATCATTCTATCTATGTTGTCAATCCCACCTCCAACCAATTCTTGGCTCTTTCGCCTCCGAGGGCTGGGAAGAGCACAGAATTCGTGCGGTATGCTCTGGCTTTTGACCCTTCCAAATCGCCTCATTACAAGGTGGTCTGCCTTAGTACTACCCGCAAGCACAATTGGGTGCACCAGGTGGATGTTTATTCGTCTGAGACTCGATCTTGGAGGCTTCTCGAGACGCCTTACGCCTCCAGGGACCTTGATATGCACTTGGATGGCAGGAGCAGGGAAGGAGGTGTGTACTGCAACGGCGCGATTCATTGGATAAGAGACGAAACAGAGCTGCCCATTCGTGTTGGTGAGGATGGAAACAGTTTGGTAAGAGAGAACGCTGATTTGGTACATTACTATGGCATTGCTGAAGAACGTCTGGGACTTGCCATTCCTAGTGCCCCTCTGGTTGTAACTGGAAACTATCCTCCTAAGTTTCCGAAATTTGATCATAGATATTTTGGGGAGTCTGGTGATCATTTGTATTTGATTGATGTATATAAGGACTCTTCGTCGAAAAATAATATATATAAGGATTGTAATGCTGAATTTGATGTCATGGAGATGGGGAAGGACTACTCTGGCTGGTTTGTCAAGTATCATGTTGATCTTAATCCCGTAGTTGCTGCATTCCGTGGCAATTATGATCACTTTTCTCGTTTTTATGTCTTGTTTCTTGGTGGAGAGGGAAAAGAAGAACAAGATCAGCAGCAGCAGCAGGAGGTTTCTTCATCTCTTTTGTTCCATGTTCCTGGCCATTCGAGTCCATCGATTGCCGAAGCCGTCTCATACGAAACCCGGCAGCGAAACCAGCGCTCCAAGATGTCAAACACTGACCAGACCACGTACCGGGTAACTTCACTTGTTCTCTTCTGTTTTCATCTGCATGTAAATCAAGAAAACTGCCGCGTACTCTTCATTGTGGTTGCCGCTATGTTTGCCAGAGAGATGGGCGAAGAAGTTAAGGGAGCAGTCGTGGTTCCGGAATCAGTTTTGAAGAAAACAAAGAGGGCAGAGGAATGGGGTCTGGCAAAGAAGGCTAAGAAATATGCACAGGAGTATGATGAACAGCAAAAGGAGTTGATCCGGTTGAAGCGTGAGGCGAAGCTGAAGGGAGGGTTTCATGTCAACCCAGAAGCCAAGCTGCTGTTCATTGTCAGGATACGTGGTATCTATGCCATTGACCCAAAAACTAAGAAGATTCTCCAGCTTCTGCGTTTGAGACAGATCTTCAATGGTGTGTTCTTGAAAGTAAACAAAGTAACATTGAACATGCTGCACCGGGTTGAACCTTATGTGACTTATGGGTATCCTAACTTGAAGAGTGTCAAGGAATTAATCTACAAGAGGGGCTATGGAAAGCTCAACAAGCAGAGAACTGCTTTGACCGACAATTCTGTTGTTGAACAGGCTTTAGGTAAGCATGGAATCATTTGCGTGGAGGACCTTATCCATGAGATCTTGACTGTTGGACCCCATTTCAAGGAGGCAAACAACTTCCTCTGGCCATTTAAGCTCAAGGCACCCCTTGGTGGTTTAAAGAAGAAGAGAAAGCACTACGTTGAGGGTGTTGATGCTGGAAATCGCGAAGATTACATCAATGAGCTCATCAGGAGGATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
RVT_3 Ribosomal_L30 Ribosomal_L30_N

Protein Analysis

727

Amino Acids

83.43

Weight (kDa)

9.34

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 144 - 281 5.3e-10 F-box associated beta propeller domain
FBA_3 PF08268 166 - 270 1e-08 F-box associated beta propeller domain
Ribosomal_L30_N PF08079 530 - 563 4.2e-09 Ribosomal L30 N-terminal domain
Ribosomal_L30 PF00327 569 - 619 8.2e-15 Ribosomal protein L30p/L7e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018022)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06960 FvH4_4g06960
rosa_chinensis RchiOBHm_Chr4g0399001
rosa_laevigata RLG00000009302
rosa_multiflora Rmu_sc0003033.1_g000018
rosa_roxburghii Rroxscaffold_5G00344060
rosa_rugosa Rorug04G0013600
rosa_samantha Rh4AG091400 Rh4BG089100 Rh4DG084200
rosa_wichuraiana Rw4G007570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2080
AccB7I CCANNNNNTGG 2 cut(s) 159, 565
AccII CGCG 4 cut(s) 65, 821, 1467, 2145
AccIII TCCGGA 1 cut(s) 1540
AciI CCGC 8 cut(s) 60, 63, 65, 196, 610, 676, 1465, 1488
AclWI GGATC 1 cut(s) 1636
AcoI YGGCCR 2 cut(s) 1312, 2063
AcsI RAATTY 3 cut(s) 602, 1001, 1106
AcuI CTGAAG 3 cut(s) 413, 948, 1688
AcyI GRCGYC 1 cut(s) 746
AdeI CACNNNGTG 1 cut(s) 700
AfaI GTAC 5 cut(s) 670, 809, 909, 1406, 1470
AfeI AGCGCT 1 cut(s) 1373
AfiI CCNNNNNNNGG 6 cut(s) 159, 565, 587, 1407, 2041, 2090
AhdI GACNNNNNGTC 1 cut(s) 1069
AjnI CCWGG 4 cut(s) 331, 696, 758, 1309
AjuI GAANNNNNNNTTGG 6 cut(s) 146, 178, 887, 919, 1691, 1723
AluBI AGCT 9 cut(s) 48, 122, 850, 1666, 1702, 1768, 1921, 2074, 2164
AluI AGCT 9 cut(s) 48, 122, 850, 1666, 1702, 1768, 1921, 2074, 2164
Alw21I GWGCWC 3 cut(s) 599, 696, 2166
Alw26I GTCTC 8 cut(s) 143, 232, 374, 713, 737, 831, 1348, 1774
Alw44I GTGCAC 1 cut(s) 692
AlwI GGATC 1 cut(s) 1636
AlwNI CAGNNNCTG 1 cut(s) 1771
Ama87I CYCGRG 2 cut(s) 182, 740
Aor13HI TCCGGA 1 cut(s) 1540
Aor51HI AGCGCT 1 cut(s) 1373
AoxI GGCC 3 cut(s) 442, 1312, 2063
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 8 cut(s) 850, 1184, 1267, 1270, 1273, 1360, 1702, 1834
ApoI RAATTY 3 cut(s) 602, 1001, 1106
AseI ATTAAT 1 cut(s) 1895
Asp700I GAANNNNTTC 2 cut(s) 90, 105
AspLEI GCGC 2 cut(s) 821, 1374
AspS9I GGNCC 3 cut(s) 763, 2002, 2030
AsuC2I CCSGG 3 cut(s) 1357, 1409, 1841
AsuHPI GGTGA 6 cut(s) 30, 185, 253, 320, 878, 1043
AvaI CYCGRG 2 cut(s) 182, 740
AvaII GGWCC 3 cut(s) 763, 2002, 2030
BaeGI GKGCMC 2 cut(s) 696, 961
BalI TGGCCA 2 cut(s) 1314, 2065
BanI GGYRCC 1 cut(s) 2080
BanII GRGCYC 1 cut(s) 2166
BbsI GAAGAC 1 cut(s) 17
Bbv12I GWGCWC 3 cut(s) 599, 696, 2166
BbvI GCAGC 8 cut(s) 837, 1171, 1279, 1282, 1285, 1372, 1689, 1821
BccI CCATC 8 cut(s) 23, 295, 503, 776, 866, 1117, 1333, 1501
BceAI ACGGC 2 cut(s) 832, 1325
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 4 cut(s) 333, 698, 760, 1311
BciVI GTATCC 2 cut(s) 1713, 1878
BclI TGATCA 3 cut(s) 1006, 1033, 1204
BcnI CCSGG 3 cut(s) 1357, 1409, 1841
BcoDI GTCTC 8 cut(s) 143, 232, 374, 713, 737, 831, 1348, 1774
BfaI CTAG 1 cut(s) 954
BfoI RGCGCY 1 cut(s) 1375
BfuI GTATCC 2 cut(s) 1713, 1878
BglII AGATCT 2 cut(s) 1785, 2016
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 7 cut(s) 333, 698, 760, 1311, 1357, 1409, 1841
Bme18I GGWCC 3 cut(s) 763, 2002, 2030
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmeT110I CYCGRG 2 cut(s) 182, 740
BmgT120I GGNCC 3 cut(s) 763, 2002, 2030
BmiI GGNNCC 4 cut(s) 764, 1539, 2032, 2082
BmrFI CCNGG 7 cut(s) 333, 698, 760, 1311, 1357, 1409, 1841
BmsI GCATC 2 cut(s) 156, 2122
BpiI GAAGAC 1 cut(s) 17
BpmI CTGGAG 2 cut(s) 742, 1748
BpuEI CTTGAG 1 cut(s) 2060
BpuMI CCSGG 3 cut(s) 1357, 1409, 1841
Bsa29I ATCGAT 1 cut(s) 1328
BsaAI YACGTR 2 cut(s) 1404, 1724
BsaBI GATNNNNATC 1 cut(s) 1170
BsaHI GRCGYC 1 cut(s) 746
BsaJI CCNNGG 7 cut(s) 63, 331, 394, 581, 759, 1192, 2086
BsaWI WCCGGW 2 cut(s) 1540, 1644
BsaXI ACNNNNNCTCC 4 cut(s) 1358, 1388, 1518, 1548
Bsc4I CCNNNNNNNGG 6 cut(s) 159, 565, 587, 1407, 2041, 2090
Bse1I ACTGG 1 cut(s) 979
Bse3DI GCAATG 2 cut(s) 445, 921
Bse8I GATNNNNATC 1 cut(s) 1170
BseAI TCCGGA 1 cut(s) 1540
BseBI CCWGG 4 cut(s) 333, 698, 760, 1311
BseCI ATCGAT 1 cut(s) 1328
BseDI CCNNGG 7 cut(s) 63, 331, 394, 581, 759, 1192, 2086
BseGI GGATG 4 cut(s) 709, 787, 877, 2181
BseJI GATNNNNATC 1 cut(s) 1170
BseLI CCNNNNNNNGG 6 cut(s) 159, 565, 587, 1407, 2041, 2090
BseMI GCAATG 2 cut(s) 445, 921
BseMII CTCAG 2 cut(s) 35, 708
BseNI ACTGG 1 cut(s) 979
BseRI GAGGAG 2 cut(s) 43, 46
BseSI GKGCMC 2 cut(s) 696, 961
BseXI GCAGC 8 cut(s) 837, 1171, 1279, 1282, 1285, 1372, 1689, 1821
BseYI CCCAGC 1 cut(s) 587
BsgI GTGCAG 1 cut(s) 1820
Bsh1236I CGCG 4 cut(s) 65, 821, 1467, 2145
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 3 cut(s) 444, 1314, 2065
BshNI GGYRCC 1 cut(s) 2080
BshVI ATCGAT 1 cut(s) 1328
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 3 cut(s) 599, 696, 2166
BsiHKCI CYCGRG 2 cut(s) 182, 740
BsiSI CCGG 5 cut(s) 1357, 1408, 1541, 1645, 1840
BslFI GGGAC 2 cut(s) 776, 954
BslI CCNNNNNNNGG 6 cut(s) 159, 565, 587, 1407, 2041, 2090
BsmAI GTCTC 8 cut(s) 143, 232, 374, 713, 737, 831, 1348, 1774
BsmBI CGTCTC 4 cut(s) 143, 737, 831, 1348
BsmFI GGGAC 2 cut(s) 776, 954
BsmI GAATGC 2 cut(s) 456, 1187
BsnI GGCC 3 cut(s) 444, 1314, 2065
BsoBI CYCGRG 2 cut(s) 182, 740
Bsp1286I GDGCHC 4 cut(s) 599, 696, 961, 2166
Bsp13I TCCGGA 1 cut(s) 1540
Bsp68I TCGCGA 1 cut(s) 2145
BspACI CCGC 8 cut(s) 60, 63, 65, 196, 610, 676, 1465, 1488
BspANI GGCC 3 cut(s) 444, 1314, 2065
BspCNI CTCAG 2 cut(s) 36, 709
BspDI ATCGAT 1 cut(s) 1328
BspEI TCCGGA 1 cut(s) 1540
BspFNI CGCG 4 cut(s) 65, 821, 1467, 2145
BspLI GGNNCC 4 cut(s) 764, 1539, 2032, 2082
BspPI GGATC 1 cut(s) 1636
BspQI GCTCTTC 1 cut(s) 587
BspT107I GGYRCC 1 cut(s) 2080
BsrDI GCAATG 2 cut(s) 445, 921
BsrI ACTGG 1 cut(s) 979
BssECI CCNNGG 7 cut(s) 63, 331, 394, 581, 759, 1192, 2086
BssNI GRCGYC 1 cut(s) 746
BssT1I CCWWGG 2 cut(s) 394, 2086
Bst2UI CCWGG 4 cut(s) 333, 698, 760, 1311
Bst4CI ACNGT 3 cut(s) 73, 881, 2026
Bst6I CTCTTC 6 cut(s) 587, 1072, 1433, 1478, 1874, 2099
BstACI GRCGYC 1 cut(s) 746
BstBAI YACGTR 2 cut(s) 1404, 1724
BstC8I GCNNGC 2 cut(s) 120, 680
BstDEI CTNAG 6 cut(s) 44, 665, 717, 990, 1599, 1752
BstDSI CCRYGG 2 cut(s) 63, 1192
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 4 cut(s) 709, 787, 877, 2181
BstFNI CGCG 4 cut(s) 65, 821, 1467, 2145
BstH2I RGCGCY 1 cut(s) 1375
BstHHI GCGC 2 cut(s) 821, 1374
BstMAI GTCTC 8 cut(s) 143, 232, 374, 713, 737, 831, 1348, 1774
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 6 cut(s) 1273, 1369, 1496, 1595, 1927, 2071
BstNI CCWGG 4 cut(s) 333, 698, 760, 1311
BstNSI RCATGY 2 cut(s) 1448, 1834
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 7 cut(s) 331, 696, 758, 1309, 1355, 1407, 1839
BstSLI GKGCMC 2 cut(s) 696, 961
BstUI CGCG 4 cut(s) 65, 821, 1467, 2145
BstV1I GCAGC 8 cut(s) 837, 1171, 1279, 1282, 1285, 1372, 1689, 1821
BstV2I GAAGAC 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 1785, 2016
BstXI CCANNNNNNTGG 2 cut(s) 1310, 1507
BstYI RGATCY 2 cut(s) 1785, 2016
Bsu15I ATCGAT 1 cut(s) 1328
BsuI GTATCC 2 cut(s) 1713, 1878
BsuRI GGCC 3 cut(s) 444, 1314, 2065
BsuTUI ATCGAT 1 cut(s) 1328
BtgI CCRYGG 2 cut(s) 63, 1192
BtsCI GGATG 4 cut(s) 709, 787, 877, 2181
BtsIMutI CAGTG 2 cut(s) 41, 1389
BtuMI TCGCGA 1 cut(s) 2145
Cac8I GCNNGC 2 cut(s) 120, 680
CaiI CAGNNNCTG 1 cut(s) 1771
CfoI GCGC 2 cut(s) 821, 1374
Cfr13I GGNCC 3 cut(s) 763, 2002, 2030
Cfr42I CCGCGG 1 cut(s) 66
ClaI ATCGAT 1 cut(s) 1328
CseI GACGC 1 cut(s) 754
CsiI ACCWGGT 1 cut(s) 696
Csp6I GTAC 5 cut(s) 669, 808, 908, 1405, 1469
CviQI GTAC 5 cut(s) 669, 808, 908, 1405, 1469
DdeI CTNAG 6 cut(s) 44, 665, 717, 990, 1599, 1752
DraI TTTAAA 1 cut(s) 2097
DraIII CACNNNGTG 1 cut(s) 700
DriI GACNNNNNGTC 1 cut(s) 1069
EaeI YGGCCR 2 cut(s) 1312, 2063
Eam1104I CTCTTC 6 cut(s) 587, 1072, 1433, 1478, 1874, 2099
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EarI CTCTTC 6 cut(s) 587, 1072, 1433, 1478, 1874, 2099
EciI GGCGGA 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 2164
Eco130I CCWWGG 2 cut(s) 394, 2086
Eco24I GRGCYC 1 cut(s) 2166
Eco47I GGWCC 3 cut(s) 763, 2002, 2030
Eco47III AGCGCT 1 cut(s) 1373
Eco53kI GAGCTC 1 cut(s) 2164
Eco57I CTGAAG 3 cut(s) 413, 948, 1688
Eco88I CYCGRG 2 cut(s) 182, 740
Eco91I GGTNACC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 2164
EcoO109I RGGNCCY 2 cut(s) 763, 2002
EcoO65I GGTNACC 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 4 cut(s) 331, 696, 758, 1309
EcoT14I CCWWGG 2 cut(s) 394, 2086
EcoT22I ATGCAT 1 cut(s) 149
EcoT38I GRGCYC 1 cut(s) 2166
ErhI CCWWGG 2 cut(s) 394, 2086
Esp3I CGTCTC 4 cut(s) 143, 737, 831, 1348
FaqI GGGAC 2 cut(s) 776, 954
FauI CCCGC 1 cut(s) 683
FbaI TGATCA 3 cut(s) 1006, 1033, 1204
FokI GGATG 3 cut(s) 716, 794, 884
FriOI GRGCYC 1 cut(s) 2166
FspBI CTAG 1 cut(s) 954
GlaI GCGC 2 cut(s) 820, 1373
GsaI CCCAGC 1 cut(s) 591
GsuI CTGGAG 2 cut(s) 742, 1748
HaeII RGCGCY 1 cut(s) 1375
HaeIII GGCC 3 cut(s) 444, 1314, 2065
HapII CCGG 5 cut(s) 1357, 1408, 1541, 1645, 1840
HgaI GACGC 1 cut(s) 754
HhaI GCGC 2 cut(s) 821, 1374
Hin1I GRCGYC 1 cut(s) 746
Hin6I GCGC 2 cut(s) 819, 1372
HinP1I GCGC 2 cut(s) 819, 1372
HincII GTYRAC 2 cut(s) 31, 1687
HindII GTYRAC 2 cut(s) 31, 1687
HpaII CCGG 5 cut(s) 1357, 1408, 1541, 1645, 1840
HphI GGTGA 6 cut(s) 30, 185, 253, 320, 878, 1043
Hpy166II GTNNAC 5 cut(s) 31, 694, 808, 1687, 1813
Hpy188I TCNGA 5 cut(s) 209, 405, 582, 718, 999
Hpy188III TCNNGA 9 cut(s) 740, 742, 1454, 1541, 1717, 1804, 2020, 2144, 2170
Hpy8I GTNNAC 5 cut(s) 31, 694, 808, 1687, 1813
Hpy99I CGWCG 2 cut(s) 77, 1075
HpyAV CCTTC 9 cut(s) 116, 274, 335, 370, 642, 791, 1123, 1588, 1663
HpyCH4III ACNGT 3 cut(s) 73, 881, 2026
HpyCH4IV ACGT 4 cut(s) 934, 1403, 1723, 2118
HpyF10VI GCNNNNNNNGC 6 cut(s) 1273, 1369, 1496, 1595, 1927, 2071
HpyF3I CTNAG 6 cut(s) 44, 665, 717, 990, 1599, 1752
HpySE526I ACGT 4 cut(s) 934, 1403, 1723, 2118
Hsp92I GRCGYC 1 cut(s) 746
HspAI GCGC 2 cut(s) 819, 1372
Kpn2I TCCGGA 1 cut(s) 1540
Ksp22I TGATCA 3 cut(s) 1006, 1033, 1204
KspI CCGCGG 1 cut(s) 66
LguI GCTCTTC 1 cut(s) 587
LmnI GCTCC 3 cut(s) 789, 1379, 1526
Lsp1109I GCAGC 8 cut(s) 837, 1171, 1279, 1282, 1285, 1372, 1689, 1821
LweI GCATC 2 cut(s) 156, 2122
MabI ACCWGGT 1 cut(s) 696
MaeI CTAG 1 cut(s) 954
MaeII ACGT 4 cut(s) 934, 1403, 1723, 2118
MaeIII GTNAC 6 cut(s) 191, 335, 970, 1411, 1819, 1855
MfeI CAATTG 1 cut(s) 685
MflI RGATCY 2 cut(s) 1785, 2016
MhlI GDGCHC 4 cut(s) 599, 696, 961, 2166
MlsI TGGCCA 2 cut(s) 1314, 2065
MluCI AATT 9 cut(s) 560, 602, 685, 1001, 1106, 1198, 1892, 1951, 2179
MluNI TGGCCA 2 cut(s) 1314, 2065
MlyI GAGTC 5 cut(s) 36, 715, 1034, 1058, 1330
MmeI TCCRAC 2 cut(s) 579, 2008
Mox20I TGGCCA 2 cut(s) 1314, 2065
Mph1103I ATGCAT 1 cut(s) 149
MroI TCCGGA 1 cut(s) 1540
MroXI GAANNNNTTC 2 cut(s) 90, 105
MscI TGGCCA 2 cut(s) 1314, 2065
MseI TTAA 6 cut(s) 381, 1170, 1521, 1895, 2070, 2096
MslI CAYNNNNRTG 5 cut(s) 780, 915, 1121, 1443, 1994
Msp20I TGGCCA 2 cut(s) 1314, 2065
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 5 cut(s) 1357, 1408, 1541, 1645, 1840
MspR9I CCNGG 7 cut(s) 333, 698, 760, 1311, 1357, 1409, 1841
MunI CAATTG 1 cut(s) 685
Mva1269I GAATGC 2 cut(s) 456, 1187
MvaI CCWGG 4 cut(s) 333, 698, 760, 1311
MvnI CGCG 4 cut(s) 65, 821, 1467, 2145
MwoI GCNNNNNNNGC 6 cut(s) 1273, 1369, 1496, 1595, 1927, 2071
NciI CCSGG 3 cut(s) 1357, 1409, 1841
NlaIV GGNNCC 4 cut(s) 764, 1539, 2032, 2082
NmuCI GTSAC 2 cut(s) 191, 1855
NruI TCGCGA 1 cut(s) 2145
NsiI ATGCAT 1 cut(s) 149
NspI RCATGY 2 cut(s) 1448, 1834
PaeR7I CTCGAG 1 cut(s) 740
PciSI GCTCTTC 1 cut(s) 587
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 396, 1325
PctI GAATGC 2 cut(s) 456, 1187
PdmI GAANNNNTTC 2 cut(s) 90, 105
PfeI GAWTC 5 cut(s) 424, 823, 1544, 1759, 1986
PflMI CCANNNNNTGG 2 cut(s) 159, 565
Ple19I CGATCG 1 cut(s) 403
PleI GAGTC 5 cut(s) 35, 715, 1033, 1058, 1329
PpsI GAGTC 5 cut(s) 35, 715, 1033, 1058, 1329
Ppu21I YACGTR 2 cut(s) 1404, 1724
PpuMI RGGWCCY 2 cut(s) 763, 2002
PshBI ATTAAT 1 cut(s) 1895
Psp124BI GAGCTC 1 cut(s) 2166
Psp5II RGGWCCY 2 cut(s) 763, 2002
Psp6I CCWGG 4 cut(s) 331, 696, 758, 1309
PspEI GGTNACC 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 587
PspGI CCWGG 4 cut(s) 331, 696, 758, 1309
PspN4I GGNNCC 4 cut(s) 764, 1539, 2032, 2082
PspPI GGNCC 3 cut(s) 763, 2002, 2030
PspPPI RGGWCCY 2 cut(s) 763, 2002
PstNI CAGNNNCTG 1 cut(s) 1771
PsuI RGATCY 2 cut(s) 1785, 2016
PvuI CGATCG 1 cut(s) 403
RruI TCGCGA 1 cut(s) 2145
RsaI GTAC 5 cut(s) 670, 809, 909, 1406, 1470
RsaNI GTAC 5 cut(s) 669, 808, 908, 1405, 1469
RseI CAYNNNNRTG 5 cut(s) 780, 915, 1121, 1443, 1994
SacI GAGCTC 1 cut(s) 2166
SacII CCGCGG 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 587
SaqAI TTAA 6 cut(s) 381, 1170, 1521, 1895, 2070, 2096
Sau96I GGNCC 3 cut(s) 763, 2002, 2030
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 5 cut(s) 36, 715, 1034, 1058, 1330
ScrFI CCNGG 7 cut(s) 333, 698, 760, 1311, 1357, 1409, 1841
SduI GDGCHC 4 cut(s) 599, 696, 961, 2166
SexAI ACCWGGT 1 cut(s) 696
SfaNI GCATC 2 cut(s) 156, 2122
Sfr274I CTCGAG 1 cut(s) 740
Sfr303I CCGCGG 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 66
SinI GGWCC 3 cut(s) 763, 2002, 2030
SlaI CTCGAG 1 cut(s) 740
SmiMI CAYNNNNRTG 5 cut(s) 780, 915, 1121, 1443, 1994
SmlI CTYRAG 2 cut(s) 740, 2075
SmoI CTYRAG 2 cut(s) 740, 2075
Sse9I AATT 9 cut(s) 560, 602, 685, 1001, 1106, 1198, 1892, 1951, 2179
SsiI CCGC 8 cut(s) 60, 63, 65, 196, 610, 676, 1465, 1488
SspMI CTAG 1 cut(s) 954
SstI GAGCTC 1 cut(s) 2166
StyD4I CCNGG 7 cut(s) 331, 696, 758, 1309, 1355, 1407, 1839
StyI CCWWGG 2 cut(s) 394, 2086
TaaI ACNGT 3 cut(s) 73, 881, 2026
TaiI ACGT 4 cut(s) 937, 1406, 1726, 2121
TaqI TCGA 7 cut(s) 84, 124, 724, 741, 1073, 1319, 1328
TaqII GACCGA 1 cut(s) 1961
TasI AATT 9 cut(s) 560, 602, 685, 1001, 1106, 1198, 1892, 1951, 2179
TatI WGTACW 2 cut(s) 668, 807
TauI GCSGC 3 cut(s) 65, 1467, 1490
TfiI GAWTC 5 cut(s) 424, 823, 1544, 1759, 1986
Tru1I TTAA 6 cut(s) 381, 1170, 1521, 1895, 2070, 2096
Tru9I TTAA 6 cut(s) 381, 1170, 1521, 1895, 2070, 2096
TscAI CASTG 2 cut(s) 48, 1396
TseFI GTSAC 2 cut(s) 191, 1855
TseI GCWGC 8 cut(s) 850, 1184, 1267, 1270, 1273, 1360, 1702, 1834
Tsp45I GTSAC 2 cut(s) 191, 1855
TspDTI ATGAA 9 cut(s) 256, 357, 815, 1278, 1427, 1465, 1638, 1670, 1699
TspGWI ACGGA 2 cut(s) 179, 1181
TspRI CASTG 2 cut(s) 48, 1396
Van91I CCANNNNNTGG 2 cut(s) 159, 565
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 3 cut(s) 763, 2002, 2030
VspI ATTAAT 1 cut(s) 1895
XapI RAATTY 3 cut(s) 602, 1001, 1106
XceI RCATGY 2 cut(s) 1448, 1834
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XhoI CTCGAG 1 cut(s) 740
XmnI GAANNNNTTC 2 cut(s) 90, 105
XspI CTAG 1 cut(s) 954
ZrmI AGTACT 1 cut(s) 670
Zsp2I ATGCAT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.