FvH4_4g07400

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016818
Physical Location & Seq
Reverse (-)
6771522 .. 6772833
1312 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07400.t1

Sequence Viewer

Length: 690 bp
ATGTATATATATAATCAACACCTTCTTCCTTCTTTACTTCTCTCTAGTCTCTGTACCATCAACATTCAAAATATGGGTGCTTGCTTGTCTTGCATACCAAAGCCAGAAGAAACCCAGGCCAATGTGCGCGTGGTTCATCTCAACGGCTACGTAGAAGAGTTCGAGCAGCCAGTTTCGGTTGGTGAGCTTACCGGAAGGCCGCCGAAGCACTTTGTGTGCACACCAACGCAGCTCCTCTCAACTGGCTACAAGCCATTGAAGCAGGACACTCTTCTTCAGCAAGGACAAATCTACTTCCTGCTGCCCTACTCTGCATTACAGAGCGATGCTTCTCCCATGGACTTGGCTGCCGTGGTCCGGAAGCTAACGGCTTTGGGAAGAAGTGGTAGTAAGAGGAGTAATGAAGGGTTGGGAAGGTCTGGCTCGTCGAGGTCCGGTCAGATGAGTCCTATGCATTGCTCTAGTCCTGTTTGGAGTAACTATTCGTCGTCGCCGGGGAGAAATAGTCCGGGCCGGTTTGTGGAGCCGGAGGGTAGCCCTAGTCGGATGGAGATGTACGGTGCGCAGAGGTCTACAAGAGCAAGGTCATGGAAGCCACTGTTGGATACTATATCGGAGAGACAGTTGAATCGGAGAACGGAGTCGGATTTGCAGGAGAAGCATGTCGAGGCTGCCAAGTATCTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

230

Amino Acids

25.43

Weight (kDa)

9.24

Isoelectric Point (pI)

75.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 25 - 206 9.5e-33 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016818)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07400
malus_domestica MD13G1243500.v1.1 MD16G1250000.v1.1
prunus_persica Prupe.1G076900_v2.0.a1
pyrus_communis pycom13g21490
rosa_chinensis RchiOBHm_Chr4g0399841
rosa_laevigata RLG00000009238
rosa_multiflora Rmu_sc0014676.1_g000014
rosa_roxburghii Rroxscaffold_5G00344730
rosa_rugosa Rorug04G0019400
rosa_samantha Rh4BG094100 Rh4CG105800 Rh4DG090700
rosa_wichuraiana Rw4G007990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 564
AccI GTMKAC 1 cut(s) 572
AccII CGCG 1 cut(s) 129
AccIII TCCGGA 1 cut(s) 357
AciI CCGC 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 260
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 2 cut(s) 55, 557
AfiI CCNNNNNNNGG 2 cut(s) 357, 520
AgsI TTSAA 3 cut(s) 68, 259, 628
AjnI CCWGG 1 cut(s) 114
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
AluBI AGCT 3 cut(s) 187, 232, 364
AluI AGCT 3 cut(s) 187, 232, 364
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 2 cut(s) 53, 613
Alw44I GTGCAC 1 cut(s) 217
Aor13HI TCCGGA 1 cut(s) 357
AoxI GGCC 3 cut(s) 117, 197, 511
ApaLI GTGCAC 1 cut(s) 217
ApeKI GCWGC 5 cut(s) 166, 229, 301, 347, 671
AspLEI GCGC 2 cut(s) 129, 565
AspS9I GGNCC 3 cut(s) 355, 432, 511
AsuC2I CCSGG 2 cut(s) 495, 510
AsuHPI GGTGA 1 cut(s) 194
AvaII GGWCC 2 cut(s) 355, 432
BaeGI GKGCMC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 5 cut(s) 178, 241, 288, 334, 658
BccI CCATC 2 cut(s) 65, 541
BceAI ACGGC 3 cut(s) 160, 335, 384
BciT130I CCWGG 1 cut(s) 116
BciVI GTATCC 1 cut(s) 598
BcnI CCSGG 2 cut(s) 495, 510
BcoDI GTCTC 2 cut(s) 53, 613
BfaI CTAG 3 cut(s) 45, 462, 540
BfuI GTATCC 1 cut(s) 598
BisI GCNGC 6 cut(s) 167, 200, 230, 302, 348, 672
BlsI GCNGC 6 cut(s) 168, 201, 231, 303, 349, 673
Bme1390I CCNGG 3 cut(s) 116, 495, 510
Bme18I GGWCC 2 cut(s) 355, 432
BmgT120I GGNCC 3 cut(s) 355, 432, 511
BmiI GGNNCC 1 cut(s) 525
BmrFI CCNGG 3 cut(s) 116, 495, 510
BmsI GCATC 1 cut(s) 316
BpuMI CCSGG 2 cut(s) 495, 510
BsaAI YACGTR 1 cut(s) 151
BsaJI CCNNGG 4 cut(s) 114, 336, 351, 494
BsaWI WCCGGW 3 cut(s) 191, 357, 434
BsaXI ACNNNNNCTCC 4 cut(s) 490, 520, 608, 638
Bsc4I CCNNNNNNNGG 2 cut(s) 357, 520
Bse118I RCCGGY 1 cut(s) 513
Bse1I ACTGG 2 cut(s) 170, 247
Bse3DI GCAATG 1 cut(s) 454
BseAI TCCGGA 1 cut(s) 357
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 4 cut(s) 114, 336, 351, 494
BseGI GGATG 1 cut(s) 552
BseLI CCNNNNNNNGG 2 cut(s) 357, 520
BseMI GCAATG 1 cut(s) 454
BseNI ACTGG 2 cut(s) 170, 247
BseRI GAGGAG 2 cut(s) 224, 409
BseSI GKGCMC 1 cut(s) 221
BseXI GCAGC 5 cut(s) 178, 241, 288, 334, 658
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 3 cut(s) 119, 199, 513
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 7 cut(s) 192, 358, 435, 494, 509, 514, 527
BslI CCNNNNNNNGG 2 cut(s) 357, 520
BsmAI GTCTC 2 cut(s) 53, 613
BsnI GGCC 3 cut(s) 119, 199, 513
Bsp1286I GDGCHC 1 cut(s) 221
Bsp13I TCCGGA 1 cut(s) 357
Bsp19I CCATGG 1 cut(s) 336
BspACI CCGC 1 cut(s) 200
BspANI GGCC 3 cut(s) 119, 199, 513
BspEI TCCGGA 1 cut(s) 357
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 525
BsrDI GCAATG 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 513
BsrI ACTGG 2 cut(s) 170, 247
BssAI RCCGGY 1 cut(s) 513
BssECI CCNNGG 4 cut(s) 114, 336, 351, 494
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 3 cut(s) 560, 600, 624
Bst6I CTCTTC 2 cut(s) 150, 276
BstBAI YACGTR 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 82
BstDSI CCRYGG 2 cut(s) 336, 351
BstF5I GGATG 1 cut(s) 552
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 2 cut(s) 129, 565
BstMAI GTCTC 2 cut(s) 53, 613
BstMWI GCNNNNNNNGC 4 cut(s) 90, 205, 259, 658
BstNI CCWGG 1 cut(s) 116
BstNSI RCATGY 1 cut(s) 665
BstSCI CCNGG 3 cut(s) 114, 493, 508
BstSLI GKGCMC 1 cut(s) 221
BstSNI TACGTA 1 cut(s) 151
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 5 cut(s) 178, 241, 288, 334, 658
BstXI CCANNNNNNTGG 1 cut(s) 343
BsuI GTATCC 1 cut(s) 598
BsuRI GGCC 3 cut(s) 119, 199, 513
BtgI CCRYGG 2 cut(s) 336, 351
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 552
BtsIMutI CAGTG 1 cut(s) 596
Cac8I GCNNGC 1 cut(s) 82
CfoI GCGC 2 cut(s) 129, 565
Cfr10I RCCGGY 1 cut(s) 513
Cfr13I GGNCC 3 cut(s) 355, 432, 511
Csp6I GTAC 2 cut(s) 54, 556
CviAII CATG 3 cut(s) 337, 588, 662
CviQI GTAC 2 cut(s) 54, 556
DraIII CACNNNGTG 1 cut(s) 214
Eam1104I CTCTTC 2 cut(s) 150, 276
EarI CTCTTC 2 cut(s) 150, 276
Eco105I TACGTA 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 355, 432
Eco57I CTGAAG 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 3 cut(s) 340, 591, 665
FatI CATG 3 cut(s) 336, 587, 661
FblI GTMKAC 1 cut(s) 572
Fnu4HI GCNGC 6 cut(s) 167, 200, 230, 302, 348, 672
FokI GGATG 1 cut(s) 559
Fsp4HI GCNGC 6 cut(s) 167, 200, 230, 302, 348, 672
FspBI CTAG 3 cut(s) 45, 462, 540
FspI TGCGCA 1 cut(s) 564
GlaI GCGC 2 cut(s) 128, 564
GluI GCNGC 6 cut(s) 167, 200, 230, 302, 348, 672
HaeIII GGCC 3 cut(s) 119, 199, 513
HapII CCGG 7 cut(s) 192, 358, 435, 494, 509, 514, 527
HhaI GCGC 2 cut(s) 129, 565
Hin1II CATG 3 cut(s) 340, 591, 665
Hin6I GCGC 2 cut(s) 127, 563
HinP1I GCGC 2 cut(s) 127, 563
HinfI GANTC 3 cut(s) 445, 628, 641
HpaII CCGG 7 cut(s) 192, 358, 435, 494, 509, 514, 527
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 2 cut(s) 219, 573
Hpy188I TCNGA 5 cut(s) 441, 546, 616, 633, 646
Hpy188III TCNNGA 1 cut(s) 358
Hpy8I GTNNAC 2 cut(s) 219, 573
Hpy99I CGWCG 3 cut(s) 430, 490, 493
HpyAV CCTTC 5 cut(s) 32, 39, 189, 398, 408
HpyCH4III ACNGT 3 cut(s) 560, 600, 624
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 5 cut(s) 93, 219, 314, 454, 652
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 205, 259, 658
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 3 cut(s) 340, 591, 665
HspAI GCGC 2 cut(s) 127, 563
Kpn2I TCCGGA 1 cut(s) 357
LmnI GCTCC 2 cut(s) 237, 523
Lsp1109I GCAGC 5 cut(s) 178, 241, 288, 334, 658
LweI GCATC 1 cut(s) 316
MaeI CTAG 3 cut(s) 45, 462, 540
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 476
MboII GAAGA 6 cut(s) 17, 119, 167, 263, 266, 390
MhlI GDGCHC 1 cut(s) 221
MlyI GAGTC 2 cut(s) 454, 650
MmeI TCCRAC 3 cut(s) 524, 582, 624
MnlI CCTC 6 cut(s) 245, 387, 423, 523, 561, 661
Mph1103I ATGCAT 1 cut(s) 456
MroI TCCGGA 1 cut(s) 357
MspI CCGG 7 cut(s) 192, 358, 435, 494, 509, 514, 527
MspR9I CCNGG 3 cut(s) 116, 495, 510
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 4 cut(s) 90, 205, 259, 658
NciI CCSGG 2 cut(s) 495, 510
NcoI CCATGG 1 cut(s) 336
NlaIII CATG 3 cut(s) 340, 591, 665
NlaIV GGNNCC 1 cut(s) 525
NsbI TGCGCA 1 cut(s) 564
NsiI ATGCAT 1 cut(s) 456
NspI RCATGY 1 cut(s) 665
PfeI GAWTC 1 cut(s) 628
PkrI GCNGC 6 cut(s) 168, 201, 231, 303, 349, 673
PleI GAGTC 2 cut(s) 453, 649
PpsI GAGTC 2 cut(s) 453, 649
Ppu21I YACGTR 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 525
PspPI GGNCC 3 cut(s) 355, 432, 511
RsaI GTAC 2 cut(s) 55, 557
RsaNI GTAC 2 cut(s) 54, 556
SatI GCNGC 6 cut(s) 167, 200, 230, 302, 348, 672
Sau96I GGNCC 3 cut(s) 355, 432, 511
SchI GAGTC 2 cut(s) 454, 650
ScrFI CCNGG 3 cut(s) 116, 495, 510
SduI GDGCHC 1 cut(s) 221
SetI ASST 9 cut(s) 24, 153, 189, 234, 366, 419, 434, 572, 587
SfaNI GCATC 1 cut(s) 316
SinI GGWCC 2 cut(s) 355, 432
SnaBI TACGTA 1 cut(s) 151
SsiI CCGC 1 cut(s) 200
SspMI CTAG 3 cut(s) 45, 462, 540
StyD4I CCNGG 3 cut(s) 114, 493, 508
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 3 cut(s) 560, 600, 624
TaiI ACGT 1 cut(s) 153
TaqI TCGA 3 cut(s) 162, 428, 666
TauI GCSGC 1 cut(s) 202
TfiI GAWTC 1 cut(s) 628
TscAI CASTG 1 cut(s) 603
TseI GCWGC 5 cut(s) 166, 229, 301, 347, 671
TspDTI ATGAA 2 cut(s) 125, 417
TspGWI ACGGA 1 cut(s) 653
TspRI CASTG 1 cut(s) 603
VneI GTGCAC 1 cut(s) 217
VpaK11BI GGWCC 2 cut(s) 355, 432
XceI RCATGY 1 cut(s) 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 572
XspI CTAG 3 cut(s) 45, 462, 540
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.