pycom13g21490

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0016818
Physical Location & Seq
Reverse (-)
18230692 .. 18231309
618 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21490.1

Sequence Viewer

Length: 618 bp
ATGGGTGGTTGCTTTTCTTGCAGACCAAAAGTCGAACGCCAGAATGTGAAGGTGGTGCATCTGAATGGGTATGTGGAGGATTTTGAGCACCCAATCTCAGTAGGGCAAGTGACAGGGAAGCCCACAAAGCACTTTGTATGCACTCCATCTCAGCTCCTCTCAACTGGGTCGAAGCCATTGAAGCATGACACCCTCCTCCAACCAGGGCAGGTCTACTTCCTCCTCCCCTACTCTGCATTGCGGAGTGATGTTTCTCCTCTCGACTTGGCTTCCATAGCCAGAAAGCTCACTTCTGCAGCAGCAAAGAGCAGCAGGAAGCAGTCTTCGTCTGCCCCTCAGTCTCCTGCGGCCTATTCTTCAAGCATGAGCGGCCAGTTTTGCCCAAGCCCTATGTGGAGCTCTTCGTCGTCGCCGGGGAGGAGTCCGGGGAGGAGTCCAGGGAGGAGTCCGGGAAGGTTTGTGGAGCAGGAGAATTTGAGTATGATGGTGGATTATGGCTCTCAGAGATCGTCGCGTCGTTCGTGGAAGCCGCTGTTGGATACGATAACGGAGAGATCGTTGAACAAGATGACTGAGTCTGATTTGCAGGAGAGGCAAGTGGAGGTGGTTGTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

206

Amino Acids

22.44

Weight (kDa)

9.62

Isoelectric Point (pI)

78.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 184 1.2e-32 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016818)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07400
malus_domestica MD13G1243500.v1.1 MD16G1250000.v1.1
prunus_persica Prupe.1G076900_v2.0.a1
pyrus_communis pycom13g21490
rosa_chinensis RchiOBHm_Chr4g0399841
rosa_laevigata RLG00000009238
rosa_multiflora Rmu_sc0014676.1_g000014
rosa_roxburghii Rroxscaffold_5G00344730
rosa_rugosa Rorug04G0019400
rosa_samantha Rh4BG094100 Rh4CG105800 Rh4DG090700
rosa_wichuraiana Rw4G007990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
AccBSI CCGCTC 1 cut(s) 369
AccI GTMKAC 1 cut(s) 213
AccII CGCG 1 cut(s) 514
AciI CCGC 4 cut(s) 241, 347, 369, 530
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 1 cut(s) 472
AgsI TTSAA 3 cut(s) 181, 360, 562
AhdI GACNNNNNGTC 1 cut(s) 29
AjnI CCWGG 2 cut(s) 202, 436
AjuI GAANNNNNNNTTGG 2 cut(s) 518, 550
AluBI AGCT 3 cut(s) 154, 286, 399
AluI AGCT 3 cut(s) 154, 286, 399
Alw21I GWGCWC 2 cut(s) 90, 401
Alw26I GTCTC 1 cut(s) 345
AoxI GGCC 2 cut(s) 348, 370
ApeKI GCWGC 3 cut(s) 296, 299, 309
ApoI RAATTY 1 cut(s) 472
AsuC2I CCSGG 3 cut(s) 414, 426, 450
BanII GRGCYC 1 cut(s) 401
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 2 cut(s) 90, 401
BbvI GCAGC 3 cut(s) 308, 311, 321
BccI CCATC 2 cut(s) 154, 478
BciT130I CCWGG 2 cut(s) 204, 438
BciVI GTATCC 1 cut(s) 532
BcnI CCSGG 3 cut(s) 414, 426, 450
BcoDI GTCTC 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 294
BfuAI ACCTGC 1 cut(s) 199
BfuI GTATCC 1 cut(s) 532
BisI GCNGC 6 cut(s) 297, 300, 310, 348, 370, 530
BlsI GCNGC 6 cut(s) 298, 301, 311, 349, 371, 531
Bme1390I CCNGG 5 cut(s) 204, 414, 426, 438, 450
BmeRI GACNNNNNGTC 1 cut(s) 29
BmrFI CCNGG 5 cut(s) 204, 414, 426, 438, 450
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 67
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 315
BpuMI CCSGG 3 cut(s) 414, 426, 450
BsaJI CCNNGG 4 cut(s) 203, 413, 425, 437
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bse1I ACTGG 2 cut(s) 169, 373
Bse3DI GCAATG 1 cut(s) 236
BseBI CCWGG 2 cut(s) 204, 438
BseDI CCNNGG 4 cut(s) 203, 413, 425, 437
BseMI GCAATG 1 cut(s) 236
BseMII CTCAG 5 cut(s) 111, 164, 350, 515, 564
BseNI ACTGG 2 cut(s) 169, 373
BseRI GAGGAG 7 cut(s) 146, 185, 212, 246, 433, 445, 457
BseXI GCAGC 3 cut(s) 308, 311, 321
Bsh1236I CGCG 1 cut(s) 514
BshFI GGCC 2 cut(s) 350, 372
BsiHKAI GWGCWC 2 cut(s) 90, 401
BsiSI CCGG 3 cut(s) 413, 425, 449
BsmAI GTCTC 1 cut(s) 345
BsnI GGCC 2 cut(s) 350, 372
Bsp1286I GDGCHC 2 cut(s) 90, 401
Bsp143I GATC 2 cut(s) 506, 554
BspACI CCGC 4 cut(s) 241, 347, 369, 530
BspANI GGCC 2 cut(s) 350, 372
BspCNI CTCAG 5 cut(s) 110, 163, 349, 514, 565
BspFNI CGCG 1 cut(s) 514
BspMAI CTGCAG 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 199
BspQI GCTCTTC 1 cut(s) 406
BsrBI CCGCTC 1 cut(s) 369
BsrDI GCAATG 1 cut(s) 236
BsrI ACTGG 2 cut(s) 169, 373
BssECI CCNNGG 4 cut(s) 203, 413, 425, 437
BssMI GATC 2 cut(s) 506, 554
Bst2UI CCWGG 2 cut(s) 204, 438
Bst6I CTCTTC 1 cut(s) 406
BstDEI CTNAG 5 cut(s) 97, 150, 336, 501, 573
BstFNI CGCG 1 cut(s) 514
BstKTI GATC 2 cut(s) 509, 557
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 2 cut(s) 506, 554
BstMWI GCNNNNNNNGC 7 cut(s) 18, 127, 181, 275, 369, 378, 592
BstNI CCWGG 2 cut(s) 204, 438
BstSCI CCNGG 5 cut(s) 202, 412, 424, 436, 448
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 514
BstV1I GCAGC 3 cut(s) 308, 311, 321
BstV2I GAAGAC 1 cut(s) 315
BsuI GTATCC 1 cut(s) 532
BsuRI GGCC 2 cut(s) 350, 372
BveI ACCTGC 1 cut(s) 199
CseI GACGC 1 cut(s) 503
CviAII CATG 2 cut(s) 185, 364
DdeI CTNAG 5 cut(s) 97, 150, 336, 501, 573
DpnI GATC 2 cut(s) 508, 556
DpnII GATC 2 cut(s) 506, 554
DriI GACNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 406
Eam1105I GACNNNNNGTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 406
Ecl136II GAGCTC 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 401
Eco53kI GAGCTC 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 399
EcoRII CCWGG 2 cut(s) 202, 436
EcoT38I GRGCYC 1 cut(s) 401
FaeI CATG 2 cut(s) 188, 367
FaiI YATR 8 cut(s) 72, 139, 186, 275, 365, 392, 482, 495
FatI CATG 2 cut(s) 184, 363
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 6 cut(s) 297, 300, 310, 348, 370, 530
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 6 cut(s) 297, 300, 310, 348, 370, 530
GluI GCNGC 6 cut(s) 297, 300, 310, 348, 370, 530
HaeIII GGCC 2 cut(s) 350, 372
HapII CCGG 3 cut(s) 413, 425, 449
HgaI GACGC 1 cut(s) 503
Hin1II CATG 2 cut(s) 188, 367
HinfI GANTC 4 cut(s) 421, 433, 445, 575
HpaII CCGG 3 cut(s) 413, 425, 449
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 3 cut(s) 63, 504, 580
Hpy188III TCNNGA 1 cut(s) 260
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 4 cut(s) 409, 412, 514, 519
HpyAV CCTTC 2 cut(s) 43, 447
HpyCH4V TGCA 6 cut(s) 21, 58, 141, 236, 296, 586
HpyF10VI GCNNNNNNNGC 7 cut(s) 18, 127, 181, 275, 369, 378, 592
HpyF3I CTNAG 5 cut(s) 97, 150, 336, 501, 573
Hsp92II CATG 2 cut(s) 188, 367
Kzo9I GATC 2 cut(s) 506, 554
LguI GCTCTTC 1 cut(s) 406
LmnI GCTCC 3 cut(s) 159, 396, 463
Lsp1109I GCAGC 3 cut(s) 308, 311, 321
LweI GCATC 1 cut(s) 67
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 508, 556
MbiI CCGCTC 1 cut(s) 369
MboI GATC 2 cut(s) 506, 554
MboII GAAGA 3 cut(s) 315, 348, 393
MhlI GDGCHC 2 cut(s) 90, 401
MluCI AATT 2 cut(s) 472, 613
MlyI GAGTC 4 cut(s) 430, 442, 454, 584
MmeI TCCRAC 2 cut(s) 223, 516
MslI CAYNNNNRTG 1 cut(s) 63
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 3 cut(s) 413, 425, 449
MspR9I CCNGG 5 cut(s) 204, 414, 426, 438, 450
MvaI CCWGG 2 cut(s) 204, 438
MvnI CGCG 1 cut(s) 514
MwoI GCNNNNNNNGC 7 cut(s) 18, 127, 181, 275, 369, 378, 592
NciI CCSGG 3 cut(s) 414, 426, 450
NdeII GATC 2 cut(s) 506, 554
NlaIII CATG 2 cut(s) 188, 367
NmuCI GTSAC 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 406
PcsI WCGNNNNNNNCGW 2 cut(s) 518, 554
PflFI GACNNNGTC 1 cut(s) 574
PfoI TCCNGGA 1 cut(s) 448
PkrI GCNGC 6 cut(s) 298, 301, 311, 349, 371, 531
PleI GAGTC 4 cut(s) 429, 441, 453, 583
PpsI GAGTC 4 cut(s) 429, 441, 453, 583
Psp124BI GAGCTC 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 202, 436
PspGI CCWGG 2 cut(s) 202, 436
PstI CTGCAG 1 cut(s) 298
PsyI GACNNNGTC 1 cut(s) 574
RseI CAYNNNNRTG 1 cut(s) 63
SacI GAGCTC 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 406
SatI GCNGC 6 cut(s) 297, 300, 310, 348, 370, 530
Sau3AI GATC 2 cut(s) 506, 554
SchI GAGTC 4 cut(s) 430, 442, 454, 584
ScrFI CCNGG 5 cut(s) 204, 414, 426, 438, 450
SduI GDGCHC 2 cut(s) 90, 401
SetI ASST 7 cut(s) 54, 156, 213, 288, 401, 458, 606
SfaNI GCATC 1 cut(s) 67
SfcI CTRYAG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 63
Sse9I AATT 2 cut(s) 472, 613
SsiI CCGC 4 cut(s) 241, 347, 369, 530
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 5 cut(s) 202, 412, 424, 436, 448
TaqI TCGA 3 cut(s) 33, 170, 261
TasI AATT 2 cut(s) 472, 613
TauI GCSGC 3 cut(s) 350, 372, 532
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 3 cut(s) 296, 299, 309
Tsp45I GTSAC 1 cut(s) 109
TspGWI ACGGA 1 cut(s) 563
Tth111I GACNNNGTC 1 cut(s) 574
XapI RAATTY 1 cut(s) 472
XcmI CCANNNNNNNNNTGG 1 cut(s) 390
XmiI GTMKAC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.