MD16G1250000.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016818
Physical Location & Seq
Reverse (-)
28003850 .. 28004482
633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1250000.v1.1.491

Sequence Viewer

Length: 633 bp
ATGGGTGGTTGCTTTTCCTGCAGACAAAAAGTTGAACTCCAGAATGTGAGGGTGGTGCATCTGAATGGGTATGTGGAGGATTTTGAGCACCCAATCTCGGTAGGGCAAGTGACAGGGAAGCCCACAAAGCACTTTGTGTGCACTCCAGTTCAGCTCCTCTCAAGTGGGTCGAAGCCATTAAAGCATGACACCGTCCTCCAACCAGGGCAGGTCTACTTCCTCCTCCCCTACTCTGCATTGCAGAGTGATGTTTCTCCTCTGGACTTGGCCTCCATTGCCAGAAAGCTCACATCTGCAGCAGCAAAGAGCAGCAGGAAGCAGTCTACGTCTGCCCCTCAGTCTCCTGTGGCTTATTCTTCAAGCATGAGCGGCCAGTTTTGCCCAAGCCCTGTGTGGAGCTCTACGTCGTCGCCGGGGAGGAGTCCGGGGAGGAGTCCAGGGAGGAGTCCAGGGAGGAGTCCGCTAAGGTTTGTGGAGCAGGAGAATTTGAGTATGATGATGGATTATGGCTCTCAGAGATCGTCGCGGGGCCGGTCATGGAAACCGCTGTTGGATACGATAACGGAGAGATCGTTGAACAAAAGGAGCGAGTCTGACTTGCAGGAGATGCATGTGGAGGTTGTTGTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

211

Amino Acids

22.98

Weight (kDa)

9.62

Isoelectric Point (pI)

83.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 189 2.4e-32 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016818)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07400
malus_domestica MD13G1243500.v1.1 MD16G1250000.v1.1
prunus_persica Prupe.1G076900_v2.0.a1
pyrus_communis pycom13g21490
rosa_chinensis RchiOBHm_Chr4g0399841
rosa_laevigata RLG00000009238
rosa_multiflora Rmu_sc0014676.1_g000014
rosa_roxburghii Rroxscaffold_5G00344730
rosa_rugosa Rorug04G0019400
rosa_samantha Rh4BG094100 Rh4CG105800 Rh4DG090700
rosa_wichuraiana Rw4G007990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
AccBSI CCGCTC 1 cut(s) 369
AccI GTMKAC 2 cut(s) 213, 323
AccII CGCG 1 cut(s) 526
AciI CCGC 4 cut(s) 369, 461, 526, 545
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 1 cut(s) 484
AdeI CACNNNGTG 1 cut(s) 136
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 3 cut(s) 35, 360, 577
AjnI CCWGG 3 cut(s) 202, 436, 448
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
AluBI AGCT 3 cut(s) 154, 286, 399
AluI AGCT 3 cut(s) 154, 286, 399
Alw21I GWGCWC 3 cut(s) 90, 143, 401
Alw26I GTCTC 1 cut(s) 345
Alw44I GTGCAC 1 cut(s) 139
AoxI GGCC 3 cut(s) 267, 370, 529
ApaLI GTGCAC 1 cut(s) 139
ApeKI GCWGC 3 cut(s) 296, 299, 309
ApoI RAATTY 1 cut(s) 484
AspS9I GGNCC 1 cut(s) 529
AsuC2I CCSGG 2 cut(s) 414, 426
BaeGI GKGCMC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 401
Bbv12I GWGCWC 3 cut(s) 90, 143, 401
BbvI GCAGC 3 cut(s) 308, 311, 321
BccI CCATC 1 cut(s) 493
BciT130I CCWGG 3 cut(s) 204, 438, 450
BciVI GTATCC 1 cut(s) 547
BcnI CCSGG 2 cut(s) 414, 426
BcoDI GTCTC 1 cut(s) 345
BfmI CTRYAG 2 cut(s) 19, 294
BfuAI ACCTGC 1 cut(s) 199
BfuI GTATCC 1 cut(s) 547
BisI GCNGC 4 cut(s) 297, 300, 310, 370
BlsI GCNGC 4 cut(s) 298, 301, 311, 371
Bme1390I CCNGG 5 cut(s) 204, 414, 426, 438, 450
BmgT120I GGNCC 1 cut(s) 529
BmiI GGNNCC 1 cut(s) 530
BmrFI CCNGG 5 cut(s) 204, 414, 426, 438, 450
BmsI GCATC 2 cut(s) 67, 597
BpmI CTGGAG 2 cut(s) 23, 129
Bpu10I CCTNAGC 1 cut(s) 464
BpuEI CTTGAG 1 cut(s) 145
BpuMI CCSGG 2 cut(s) 414, 426
BsaJI CCNNGG 5 cut(s) 203, 413, 425, 437, 449
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 557, 587
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 2 cut(s) 146, 373
Bse3DI GCAATG 2 cut(s) 236, 273
BseBI CCWGG 3 cut(s) 204, 438, 450
BseDI CCNNGG 5 cut(s) 203, 413, 425, 437, 449
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 2 cut(s) 236, 273
BseMII CTCAG 2 cut(s) 350, 527
BseNI ACTGG 2 cut(s) 146, 373
BseRI GAGGAG 7 cut(s) 146, 212, 246, 433, 445, 457, 469
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 3 cut(s) 308, 311, 321
Bsh1236I CGCG 1 cut(s) 526
BshFI GGCC 3 cut(s) 269, 372, 531
BsiHKAI GWGCWC 3 cut(s) 90, 143, 401
BsiSI CCGG 3 cut(s) 413, 425, 532
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 1 cut(s) 345
BsnI GGCC 3 cut(s) 269, 372, 531
Bsp1286I GDGCHC 3 cut(s) 90, 143, 401
Bsp143I GATC 2 cut(s) 518, 569
BspACI CCGC 4 cut(s) 369, 461, 526, 545
BspANI GGCC 3 cut(s) 269, 372, 531
BspCNI CTCAG 2 cut(s) 349, 526
BspFNI CGCG 1 cut(s) 526
BspLI GGNNCC 1 cut(s) 530
BspMAI CTGCAG 2 cut(s) 23, 298
BspMI ACCTGC 1 cut(s) 199
BsrBI CCGCTC 1 cut(s) 369
BsrDI GCAATG 2 cut(s) 236, 273
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 2 cut(s) 146, 373
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 5 cut(s) 203, 413, 425, 437, 449
BssMI GATC 2 cut(s) 518, 569
Bst2UI CCWGG 3 cut(s) 204, 438, 450
Bst4CI ACNGT 1 cut(s) 193
BstAPI GCANNNNNTGC 1 cut(s) 607
BstDEI CTNAG 3 cut(s) 336, 464, 513
BstFNI CGCG 1 cut(s) 526
BstKTI GATC 2 cut(s) 521, 572
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 2 cut(s) 518, 569
BstMWI GCNNNNNNNGC 7 cut(s) 18, 127, 181, 275, 369, 378, 607
BstNI CCWGG 3 cut(s) 204, 438, 450
BstNSI RCATGY 1 cut(s) 614
BstSCI CCNGG 5 cut(s) 202, 412, 424, 436, 448
BstSFI CTRYAG 2 cut(s) 19, 294
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 1 cut(s) 526
BstV1I GCAGC 3 cut(s) 308, 311, 321
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 3 cut(s) 269, 372, 531
BveI ACCTGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 529
CviAII CATG 4 cut(s) 185, 364, 537, 611
DdeI CTNAG 3 cut(s) 336, 464, 513
DpnI GATC 2 cut(s) 520, 571
DpnII GATC 2 cut(s) 518, 569
DraIII CACNNNGTG 1 cut(s) 136
EaeI YGGCCR 1 cut(s) 370
Ecl136II GAGCTC 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 401
Eco53kI GAGCTC 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 399
EcoRII CCWGG 3 cut(s) 202, 436, 448
EcoT22I ATGCAT 1 cut(s) 612
EcoT38I GRGCYC 1 cut(s) 401
FaeI CATG 4 cut(s) 188, 367, 540, 614
FaiI YATR 7 cut(s) 72, 186, 365, 494, 507, 538, 612
FatI CATG 4 cut(s) 184, 363, 536, 610
FauI CCCGC 1 cut(s) 519
FblI GTMKAC 2 cut(s) 213, 323
Fnu4HI GCNGC 4 cut(s) 297, 300, 310, 370
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 4 cut(s) 297, 300, 310, 370
GluI GCNGC 4 cut(s) 297, 300, 310, 370
GsuI CTGGAG 2 cut(s) 23, 129
HaeIII GGCC 3 cut(s) 269, 372, 531
HapII CCGG 3 cut(s) 413, 425, 532
Hin1II CATG 4 cut(s) 188, 367, 540, 614
HinfI GANTC 5 cut(s) 421, 433, 445, 457, 590
HpaII CCGG 3 cut(s) 413, 425, 532
Hpy166II GTNNAC 3 cut(s) 141, 214, 324
Hpy188I TCNGA 3 cut(s) 63, 516, 595
Hpy188III TCNNGA 2 cut(s) 40, 260
Hpy8I GTNNAC 3 cut(s) 141, 214, 324
Hpy99I CGWCG 3 cut(s) 409, 412, 526
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 2 cut(s) 326, 404
HpyCH4V TGCA 8 cut(s) 21, 58, 141, 236, 241, 296, 601, 610
HpyF10VI GCNNNNNNNGC 7 cut(s) 18, 127, 181, 275, 369, 378, 607
HpyF3I CTNAG 3 cut(s) 336, 464, 513
HpySE526I ACGT 2 cut(s) 326, 404
Hsp92II CATG 4 cut(s) 188, 367, 540, 614
Kzo9I GATC 2 cut(s) 518, 569
LmnI GCTCC 4 cut(s) 159, 396, 475, 585
Lsp1109I GCAGC 3 cut(s) 308, 311, 321
LweI GCATC 2 cut(s) 67, 597
MaeII ACGT 2 cut(s) 326, 404
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 520, 571
MbiI CCGCTC 1 cut(s) 369
MboI GATC 2 cut(s) 518, 569
MboII GAAGA 1 cut(s) 348
MhlI GDGCHC 3 cut(s) 90, 143, 401
MluCI AATT 1 cut(s) 484
MlyI GAGTC 5 cut(s) 430, 442, 454, 466, 599
MmeI TCCRAC 2 cut(s) 223, 531
Mph1103I ATGCAT 1 cut(s) 612
MseI TTAA 1 cut(s) 179
MslI CAYNNNNRTG 1 cut(s) 63
MspA1I CMGCKG 1 cut(s) 547
MspI CCGG 3 cut(s) 413, 425, 532
MspR9I CCNGG 5 cut(s) 204, 414, 426, 438, 450
MvaI CCWGG 3 cut(s) 204, 438, 450
MvnI CGCG 1 cut(s) 526
MwoI GCNNNNNNNGC 7 cut(s) 18, 127, 181, 275, 369, 378, 607
NciI CCSGG 2 cut(s) 414, 426
NdeII GATC 2 cut(s) 518, 569
NlaIII CATG 4 cut(s) 188, 367, 540, 614
NlaIV GGNNCC 1 cut(s) 530
NmuCI GTSAC 1 cut(s) 109
NsiI ATGCAT 1 cut(s) 612
NspI RCATGY 1 cut(s) 614
PcsI WCGNNNNNNNCGW 1 cut(s) 569
PflFI GACNNNGTC 1 cut(s) 191
PkrI GCNGC 4 cut(s) 298, 301, 311, 371
PleI GAGTC 5 cut(s) 429, 441, 453, 465, 598
PpsI GAGTC 5 cut(s) 429, 441, 453, 465, 598
Psp124BI GAGCTC 1 cut(s) 401
Psp6I CCWGG 3 cut(s) 202, 436, 448
PspGI CCWGG 3 cut(s) 202, 436, 448
PspN4I GGNNCC 1 cut(s) 530
PspPI GGNCC 1 cut(s) 529
PstI CTGCAG 2 cut(s) 23, 298
PsyI GACNNNGTC 1 cut(s) 191
RseI CAYNNNNRTG 1 cut(s) 63
SacI GAGCTC 1 cut(s) 401
SaqAI TTAA 1 cut(s) 179
SatI GCNGC 4 cut(s) 297, 300, 310, 370
Sau3AI GATC 2 cut(s) 518, 569
Sau96I GGNCC 1 cut(s) 529
SchI GAGTC 5 cut(s) 430, 442, 454, 466, 599
ScrFI CCNGG 5 cut(s) 204, 414, 426, 438, 450
SduI GDGCHC 3 cut(s) 90, 143, 401
SetI ASST 8 cut(s) 156, 213, 288, 329, 401, 407, 470, 621
SfaNI GCATC 2 cut(s) 67, 597
SfcI CTRYAG 2 cut(s) 19, 294
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 1 cut(s) 484
SsiI CCGC 4 cut(s) 369, 461, 526, 545
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 5 cut(s) 202, 412, 424, 436, 448
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 2 cut(s) 329, 407
TaqI TCGA 1 cut(s) 170
TasI AATT 1 cut(s) 484
TauI GCSGC 1 cut(s) 372
Tru1I TTAA 1 cut(s) 179
Tru9I TTAA 1 cut(s) 179
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 3 cut(s) 296, 299, 309
Tsp45I GTSAC 1 cut(s) 109
TspGWI ACGGA 1 cut(s) 578
Tth111I GACNNNGTC 1 cut(s) 191
VneI GTGCAC 1 cut(s) 139
XapI RAATTY 1 cut(s) 484
XceI RCATGY 1 cut(s) 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 390
XmiI GTMKAC 2 cut(s) 213, 323
Zsp2I ATGCAT 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.