Rh4DG090700

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0016818
Physical Location & Seq
Reverse (-)
13688209 .. 13688829
621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG090700.1

Sequence Viewer

Length: 621 bp
ATGGGTGCTTGCTTTTCTTGTAGACCAAAGCCAGAAGAAACCCAGAACAATGTGCGCGTGGTTCATCTCAACGGCTACGTCGAAGAGTTCGAGCACCCAGTTTCGGTCAGTGAACTAACCGGAAGGCCCCCGAAGCACTTTGTGTGCACACCAGCCCAGCTCCTCTCAACTGGGTACAAGCCATTGAAGCAGGACACTCTTCTTCAGCAAGGCCAAATCTACTTCTTGCTGCCCTACTCTGCGTTACAGAGTGATGCTTCTCCTATGGACTTGGCTGCCGTGGTCCGGAAGCTAACGGCTTTGGCAAGAAGTGGTAGTAGTAGGAGTAATGAGGGGTTGGGAAGGTCTGGTAGTTCGTCAAGGTCCGGTCAGATGAGTCCTATGCATTGCTCTAGTCCTGTTTGGAGTAACTATTCATCATCGCCGGGGAGAAATAGTCCGGGCAGGTTTGTGGAGTCGGAGGGTAGTCCTAGTCGGATGGAGATGTATGGTGCGCAGAGGTCTACAAGAGCAAGGTCATGGAAGCCACTGTTGGATACTATAACGGAGAGGCAGTTGAACCGGAGAACGGAGTCGGATTTGCAGGAGAAGCATGTGGAGGCAGCCAAATATCTACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

206

Amino Acids

22.85

Weight (kDa)

9.27

Isoelectric Point (pI)

80.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 183 1.7e-32 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016818)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07400
malus_domestica MD13G1243500.v1.1 MD16G1250000.v1.1
prunus_persica Prupe.1G076900_v2.0.a1
pyrus_communis pycom13g21490
rosa_chinensis RchiOBHm_Chr4g0399841
rosa_laevigata RLG00000009238
rosa_multiflora Rmu_sc0014676.1_g000014
rosa_roxburghii Rroxscaffold_5G00344730
rosa_rugosa Rorug04G0019400
rosa_samantha Rh4BG094100 Rh4CG105800 Rh4DG090700
rosa_wichuraiana Rw4G007990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 495
Acc36I ACCTGC 1 cut(s) 435
AccI GTMKAC 2 cut(s) 22, 503
AccII CGCG 1 cut(s) 57
AccIII TCCGGA 1 cut(s) 285
AcuI CTGAAG 1 cut(s) 188
AdeI CACNNNGTG 1 cut(s) 142
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 3 cut(s) 103, 285, 568
AgsI TTSAA 2 cut(s) 187, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 515, 547
AluBI AGCT 2 cut(s) 160, 292
AluI AGCT 2 cut(s) 160, 292
Alw21I GWGCWC 2 cut(s) 96, 149
Alw44I GTGCAC 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 285
AoxI GGCC 2 cut(s) 125, 211
ApaLI GTGCAC 1 cut(s) 145
ApeKI GCWGC 3 cut(s) 229, 275, 602
AspLEI GCGC 2 cut(s) 57, 496
AspS9I GGNCC 3 cut(s) 126, 283, 363
AsuC2I CCSGG 2 cut(s) 426, 441
AvaII GGWCC 2 cut(s) 283, 363
BaeGI GKGCMC 1 cut(s) 149
Bbv12I GWGCWC 2 cut(s) 96, 149
BbvI GCAGC 3 cut(s) 216, 262, 614
BccI CCATC 1 cut(s) 472
BceAI ACGGC 3 cut(s) 88, 263, 312
BciVI GTATCC 1 cut(s) 529
BcnI CCSGG 2 cut(s) 426, 441
BfaI CTAG 2 cut(s) 393, 471
BfmI CTRYAG 1 cut(s) 614
BfuAI ACCTGC 1 cut(s) 435
BfuI GTATCC 1 cut(s) 529
BisI GCNGC 3 cut(s) 230, 276, 603
BlsI GCNGC 3 cut(s) 231, 277, 604
Bme1390I CCNGG 2 cut(s) 426, 441
Bme18I GGWCC 2 cut(s) 283, 363
BmgT120I GGNCC 3 cut(s) 126, 283, 363
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 2 cut(s) 426, 441
BmrI ACTGGG 2 cut(s) 92, 180
BmsI GCATC 1 cut(s) 244
BmuI ACTGGG 2 cut(s) 92, 180
BpuMI CCSGG 2 cut(s) 426, 441
BsaJI CCNNGG 2 cut(s) 279, 425
BsaWI WCCGGW 4 cut(s) 119, 285, 365, 561
BsaXI ACNNNNNCTCC 4 cut(s) 421, 451, 539, 569
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 285, 568
Bse1I ACTGG 2 cut(s) 98, 175
Bse3DI GCAATG 1 cut(s) 385
BseAI TCCGGA 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 279, 425
BseGI GGATG 1 cut(s) 483
BseLI CCNNNNNNNGG 3 cut(s) 103, 285, 568
BseMI GCAATG 1 cut(s) 385
BseNI ACTGG 2 cut(s) 98, 175
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 3 cut(s) 216, 262, 614
BseYI CCCAGC 1 cut(s) 156
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 2 cut(s) 127, 213
BsiHKAI GWGCWC 2 cut(s) 96, 149
BsiSI CCGG 6 cut(s) 120, 286, 366, 425, 440, 562
BslI CCNNNNNNNGG 3 cut(s) 103, 285, 568
BsnI GGCC 2 cut(s) 127, 213
Bsp1286I GDGCHC 2 cut(s) 96, 149
Bsp13I TCCGGA 1 cut(s) 285
BspANI GGCC 2 cut(s) 127, 213
BspEI TCCGGA 1 cut(s) 285
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 128
BspMI ACCTGC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 2 cut(s) 98, 175
BssECI CCNNGG 2 cut(s) 279, 425
Bst4CI ACNGT 2 cut(s) 531, 618
Bst6I CTCTTC 2 cut(s) 78, 204
BstC8I GCNNGC 1 cut(s) 10
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 483
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 2 cut(s) 57, 496
BstMWI GCNNNNNNNGC 3 cut(s) 133, 187, 589
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 2 cut(s) 424, 439
BstSFI CTRYAG 1 cut(s) 614
BstSLI GKGCMC 1 cut(s) 149
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 3 cut(s) 216, 262, 614
BsuI GTATCC 1 cut(s) 529
BsuRI GGCC 2 cut(s) 127, 213
BtgI CCRYGG 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 115, 527
BveI ACCTGC 1 cut(s) 435
Cac8I GCNNGC 1 cut(s) 10
CfoI GCGC 2 cut(s) 57, 496
Cfr13I GGNCC 3 cut(s) 126, 283, 363
Csp6I GTAC 1 cut(s) 175
CviAII CATG 2 cut(s) 519, 593
CviQI GTAC 1 cut(s) 175
DraIII CACNNNGTG 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 78, 204
EarI CTCTTC 2 cut(s) 78, 204
Eco47I GGWCC 2 cut(s) 283, 363
Eco57I CTGAAG 1 cut(s) 188
EcoO109I RGGNCCY 1 cut(s) 126
EcoT22I ATGCAT 1 cut(s) 387
FaeI CATG 2 cut(s) 522, 596
FaiI YATR 6 cut(s) 266, 383, 489, 520, 542, 594
FatI CATG 2 cut(s) 518, 592
FblI GTMKAC 2 cut(s) 22, 503
Fnu4HI GCNGC 3 cut(s) 230, 276, 603
FokI GGATG 1 cut(s) 490
Fsp4HI GCNGC 3 cut(s) 230, 276, 603
FspBI CTAG 2 cut(s) 393, 471
FspI TGCGCA 1 cut(s) 495
GlaI GCGC 2 cut(s) 56, 495
GluI GCNGC 3 cut(s) 230, 276, 603
GsaI CCCAGC 1 cut(s) 160
HaeIII GGCC 2 cut(s) 127, 213
HapII CCGG 6 cut(s) 120, 286, 366, 425, 440, 562
HhaI GCGC 2 cut(s) 57, 496
Hin1II CATG 2 cut(s) 522, 596
Hin6I GCGC 2 cut(s) 55, 494
HinP1I GCGC 2 cut(s) 55, 494
HinfI GANTC 3 cut(s) 376, 455, 572
HpaII CCGG 6 cut(s) 120, 286, 366, 425, 440, 562
Hpy166II GTNNAC 4 cut(s) 23, 113, 147, 504
Hpy188I TCNGA 4 cut(s) 372, 460, 477, 577
Hpy188III TCNNGA 1 cut(s) 286
Hpy8I GTNNAC 4 cut(s) 23, 113, 147, 504
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 117, 336
HpyCH4III ACNGT 2 cut(s) 531, 618
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 3 cut(s) 147, 385, 583
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 187, 589
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 2 cut(s) 522, 596
HspAI GCGC 2 cut(s) 55, 494
Kpn2I TCCGGA 1 cut(s) 285
LmnI GCTCC 1 cut(s) 165
Lsp1109I GCAGC 3 cut(s) 216, 262, 614
LweI GCATC 1 cut(s) 244
MaeI CTAG 2 cut(s) 393, 471
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 2 cut(s) 243, 407
MboII GAAGA 4 cut(s) 47, 95, 191, 194
MhlI GDGCHC 2 cut(s) 96, 149
MlyI GAGTC 3 cut(s) 385, 464, 581
MmeI TCCRAC 4 cut(s) 438, 455, 513, 555
MnlI CCTC 6 cut(s) 173, 325, 454, 492, 543, 592
Mph1103I ATGCAT 1 cut(s) 387
MroI TCCGGA 1 cut(s) 285
MspI CCGG 6 cut(s) 120, 286, 366, 425, 440, 562
MspR9I CCNGG 2 cut(s) 426, 441
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 3 cut(s) 133, 187, 589
NciI CCSGG 2 cut(s) 426, 441
NlaIII CATG 2 cut(s) 522, 596
NlaIV GGNNCC 1 cut(s) 128
NsbI TGCGCA 1 cut(s) 495
NsiI ATGCAT 1 cut(s) 387
NspI RCATGY 1 cut(s) 596
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 87
PkrI GCNGC 3 cut(s) 231, 277, 604
PleI GAGTC 3 cut(s) 384, 463, 580
PpsI GAGTC 3 cut(s) 384, 463, 580
PspFI CCCAGC 1 cut(s) 156
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 3 cut(s) 126, 283, 363
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SatI GCNGC 3 cut(s) 230, 276, 603
Sau96I GGNCC 3 cut(s) 126, 283, 363
SchI GAGTC 3 cut(s) 385, 464, 581
ScrFI CCNGG 2 cut(s) 426, 441
SduI GDGCHC 2 cut(s) 96, 149
SetI ASST 8 cut(s) 81, 162, 294, 347, 365, 449, 503, 518
SfaNI GCATC 1 cut(s) 244
SfcI CTRYAG 1 cut(s) 614
SinI GGWCC 2 cut(s) 283, 363
SspMI CTAG 2 cut(s) 393, 471
StyD4I CCNGG 2 cut(s) 424, 439
TaaI ACNGT 2 cut(s) 531, 618
TaiI ACGT 1 cut(s) 81
TaqI TCGA 2 cut(s) 81, 90
TaqII GACCGA 1 cut(s) 94
TscAI CASTG 2 cut(s) 115, 534
TseI GCWGC 3 cut(s) 229, 275, 602
TspDTI ATGAA 2 cut(s) 53, 405
TspGWI ACGGA 2 cut(s) 560, 584
TspRI CASTG 2 cut(s) 115, 534
VneI GTGCAC 1 cut(s) 145
VpaK11BI GGWCC 2 cut(s) 283, 363
XceI RCATGY 1 cut(s) 596
XmiI GTMKAC 2 cut(s) 22, 503
XspI CTAG 2 cut(s) 393, 471
Zsp2I ATGCAT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.