Rorug04G0019400

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016818
Physical Location & Seq
Forward (+)
2864276 .. 2867552
3277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0019400.1

Sequence Viewer

Length: 2838 bp
ATGGCTTCTCTGACCAATGAAGCAGCCAGTTCTTCTTCTTCTCCTCTCCCAAGCTCTTCTACTGATCACCAAACTCATTACACATACGAGGTCTTCTTGAGCTTTCGAGGCAAGGATACACGCTTTAATTTTACGGATCATTTGTATACTGCTCTGTGTCAGAGGGGAATTGAGACCTTCAGAGATGATAAGCTCAGCAGAGGAGAAGACATATCACAAGAGCTCCTCAAAGCAATTGACGAGTCGAGAGTTTCAATCATAGTCTTCTCTCAAAATTATGCTGCCTCAAGGTGGTGCTTAGATGAACTGGTCAAGATACTTGAATGCAGAGAATCCAAAGGACAGGAGGTTAGAGCGGTGTTCTACAAGGTGGATCCCTCAGATGTACGACACCAAAGAGGTGCTTTGGGTGACGCGTTTGCTACCCTTGATCAATGCAAATACAAGGACAGCATGGAAAAATGGAAGGCAGCTCTCAAGGAAGCAGCAGATTTGTCTGGATGGCCTTTCAAGGATGGCGAGTACGAGTCTAAATTTATCAACGACATTGTTGGGGAGTTGTCTGCCCGAGTGGTAAACCCTTCATGTGACTTGCAAGTAGCTGCACATCCTATTGGAATAGAGTCTTGTAGACAAGATGTCAACAAACTTTTACACACCGGGGAAAATATTGTTCGCATGGTAGGTATATGGGGGCCTGGTGGAATAGGAAAGACCACAATTGCGAAAGATGTGTTTAATTCTATTTGCCAAAAGTTTGAAGCTAGTTGTTTCTTGGCAGATGTCAGATCTAATTCAAATGGTCTAGCCCAACTACAAGAGACACTTTTGTCTGATATTTTAGACTCAACTTTGAAGGTAAGTAGTGTTGATCAAGGAGTCAGTTTAATAAAGACAAGGATGCGACATAAAAAAGTTCTCTTAATCCTTGATGACGTATCTCATTCTAGCCAATTACATAACTTAGTTCCGTCTCCAAATTGTTTTGGGCCGGGCAGCAGAATTCTCATAACAACAAGAGATAAACGTTGGCTAATTGCTCATCAAGTCGATGAAGTATACGAGGTCAAGATGTTAGATTATGATCAAGCTTTGGAGTTGTTTAGCTTAAATGCTTTCAAAAGAAATGGACCTCCAGACGATTATCTGGAACTTGCACAACGTGCTGTACGTTATGCTCAGGGCCTTCCATTTGGTTTGATAGTTTTAGGGTCTCATCTATTTCGTAGAAGCAGAGAGGAGTGGGAAGCTACATTAGATAGTTGTAAAGAAGATCCCCACAGAGAGATAAGAGACATTCTCAAAATAAGTTATGATGCTCTGGGAGTAGATTTAAAAGGATATTTTCTTGATATCGCTTGTTTCTTTAAAGGTGAGTATGTAGACCGTGTGAATCAAATACTAGAAGCTTGCTACGACCTCAAATCAGTGACTGGTATTGCACAACTCCAAGAAAAAGCTTTGATTAGAATTGACGGAGTGCGTTGGACAAATGGAATAGACAAAGGTCGCATTTGGATGCATGACTTGATAGAAGAAATGGGTAAAGACATAGTGTATCAAGAGTCACCTGGTGAGCCCGGGGAACGTAGCAGAGTGTGGAGCAAAGAAGATGTCGACCACGTTCTAACAAATAATACAGGCACAGATAAAATTATAGGCATCCAGTTCTCGGAATATTCTAAGATGTCTTTGAATGCTAAAAGCTTCTCAGGGATGAAGAATCTTAGATATATTTCTATGAGCAGAATGATGAAAGATGAATGCTTTTCTGGAGACATTGATTATCTTTCCAACCAGTTGAGATGGCTTGATTGGTCTAAATGTCCGTTGCAATCTTTTCCATCTGATTTTCATGCAAACAAACTTGTAAATCTCGATATTTCTGAGAGTCACGGAATCACACGACTATGGGAGGGACGTAAGAATTTTTCACGGCTAACATGCATGAATTTAAGGGGTTGTCATTCCCTAATAGAACTCCCAGACTTTAGTGGAATCCCCAACTTGAAAGAGTTGAATCTATATGAATGTCGCAATTTAGTTGAGGTTCCTGATTCCGTTGGATTCCTACGAAACCTTTTTACCTTGAATGTTGACGGCTGCTCTAACCTTAGTATGTTTCCGAGAAAAATCAACTTTAAATATGTAGAAACTATTGTTATCCGCTCTTGCAAGCTTGAGGAATTTTCAGAAGTTGGGAAAGAGATGGGTTCCTTGAGAAGTTTGGATCTATCACGCACTTGCATTAAAGAATTGGATGAGTCGATTGGAAATCTCGTTGGGCTTGAATGGTTGGATCTTAAGGATTGCAAAAATCTTACAACTCTACCGTGCAGCATTTATGGATTGCAAAATTTAAAGATATTTGAGGTTAGTAACTGCTCGAAACTTGTCAGATTTCCAACAAATACCAAAATTTTGAATGTCGATGGTTGCCCATTATCACTTCCAAAGCTAGAGTGGTTCGACATCGAAGGATGCAGTTTACCAGATTGTGATTTCCTCGAGACTCTTGATTGCTGGGAAACATTATTCCTGCTTGATCTGTCATTTAACAATTTTGTTAGTCTTCCTGCTTGCTTCACCAAATTTGTCAACTTGCAGGTGCTTTTCTTGTGGGGTTGCAAGAGACTCCGAAAACTTCCAGAGCTTCCACCAAATGTGTCAATAAACAGTTTTGTTAGTCTTCCTGCTTGCATCACCAAATTTATCAACTTGCGGGTTCTTTTCTTGGAGGGTTGCAAGAGACTTCGAGGAATTCCGGAGCTTCCCCCAAACGTTTACCATACATCCAATGGTCTTGTTTTTAATTGGTTGAGAGATTCTCGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

945

Amino Acids

107.41

Weight (kDa)

5.83

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 29 - 190 1.7e-53 TIR domain
TIR_2 PF13676 31 - 125 1.5e-14 TIR domain
NB-ARC PF00931 215 - 375 1.1e-20 NB-ARC domain
WHD_ROQ1 PF23282 446 - 523 3.4e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 707 - 798 2.1e-09 Leucine-rich repeat region
LRR_At1g61320_AtMIF1 PF23622 718 - 798 9.3e-06 At1g61320/AtMIF1, LRR domain
LRR_13 PF23286 725 - 884 8.1e-18 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016818)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07400
malus_domestica MD13G1243500.v1.1 MD16G1250000.v1.1
prunus_persica Prupe.1G076900_v2.0.a1
pyrus_communis pycom13g21490
rosa_chinensis RchiOBHm_Chr4g0399841
rosa_laevigata RLG00000009238
rosa_multiflora Rmu_sc0014676.1_g000014
rosa_roxburghii Rroxscaffold_5G00344730
rosa_rugosa Rorug04G0019400
rosa_samantha Rh4BG094100 Rh4CG105800 Rh4DG090700
rosa_wichuraiana Rw4G007990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2596
AasI GACNNNNNNGTC 1 cut(s) 829
Acc36I ACCTGC 1 cut(s) 2596
AccBSI CCGCTC 2 cut(s) 356, 2169
AccI GTMKAC 5 cut(s) 146, 631, 1059, 1383, 1617
AccII CGCG 1 cut(s) 416
AccIII TCCGGA 1 cut(s) 2764
AciI CCGC 3 cut(s) 356, 2167, 2722
AclI AACGTT 2 cut(s) 1027, 2781
AclWI GGATC 6 cut(s) 144, 368, 381, 1268, 2238, 2307
AcuI CTGAAG 1 cut(s) 163
AdeI CACNNNGTG 2 cut(s) 1163, 1574
AfaI GTAC 3 cut(s) 387, 524, 1170
AfiI CCNNNNNNNGG 2 cut(s) 291, 1672
AflII CTTAAG 1 cut(s) 2303
AflIII ACRYGT 1 cut(s) 414
AhdI GACNNNNNGTC 1 cut(s) 638
AjnI CCWGG 2 cut(s) 697, 1570
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 9 cut(s) 167, 815, 978, 1218, 1287, 1770, 2504, 2626, 2743
AlwI GGATC 6 cut(s) 144, 368, 381, 1268, 2238, 2307
AlwNI CAGNNNCTG 1 cut(s) 1433
Ama87I CYCGRG 3 cut(s) 567, 1580, 2507
Aor13HI TCCGGA 1 cut(s) 2764
AoxI GGCC 4 cut(s) 503, 695, 989, 1183
ApeKI GCWGC 8 cut(s) 23, 281, 470, 485, 602, 996, 2103, 2337
ArsI GACNNNNNNTTYG 2 cut(s) 1599, 1631
AspS9I GGNCC 4 cut(s) 695, 989, 1130, 1183
AsuC2I CCSGG 4 cut(s) 661, 993, 1581, 1582
AsuHPI GGTGA 7 cut(s) 59, 422, 1385, 1560, 1586, 2578, 2695
AvaI CYCGRG 3 cut(s) 567, 1580, 2507
AvaII GGWCC 1 cut(s) 1130
BamHI GGATCC 1 cut(s) 373
BanII GRGCYC 2 cut(s) 225, 1581
BbsI GAAGAC 5 cut(s) 85, 213, 256, 2564, 2681
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 8 cut(s) 35, 268, 482, 497, 589, 1008, 2090, 2349
BccI CCATC 6 cut(s) 495, 509, 1800, 1852, 2203, 2426
BceAI ACGGC 2 cut(s) 1952, 2116
BciT130I CCWGG 2 cut(s) 699, 1572
BciVI GTATCC 1 cut(s) 109
BclI TGATCA 4 cut(s) 64, 430, 871, 1084
BcnI CCSGG 4 cut(s) 661, 993, 1581, 1582
BcoDI GTCTC 9 cut(s) 167, 815, 978, 1218, 1287, 1770, 2504, 2626, 2743
BfaI CTAG 5 cut(s) 765, 806, 948, 1403, 2459
BfrI CTTAAG 1 cut(s) 2303
BfuAI ACCTGC 1 cut(s) 2596
BfuI GTATCC 1 cut(s) 109
BglII AGATCT 1 cut(s) 788
BisI GCNGC 8 cut(s) 24, 282, 471, 486, 603, 997, 2104, 2338
BlpI GCTNAGC 1 cut(s) 194
BlsI GCNGC 8 cut(s) 25, 283, 472, 487, 604, 998, 2105, 2339
Bme1390I CCNGG 6 cut(s) 661, 699, 993, 1572, 1581, 1582
Bme18I GGWCC 1 cut(s) 1130
BmeRI GACNNNNNGTC 1 cut(s) 638
BmeT110I CYCGRG 3 cut(s) 567, 1580, 2507
BmgT120I GGNCC 4 cut(s) 695, 989, 1130, 1183
BmiI GGNNCC 4 cut(s) 375, 696, 2052, 2215
BmrFI CCNGG 6 cut(s) 661, 699, 993, 1572, 1581, 1582
BmsI GCATC 6 cut(s) 891, 1306, 1509, 1671, 2471, 2709
BoxI GACNNNNGTC 1 cut(s) 1506
BpiI GAAGAC 5 cut(s) 85, 213, 256, 2564, 2681
BplI GAGNNNNNCTC 3 cut(s) 1284, 1316, 2812
BpmI CTGGAG 2 cut(s) 1119, 1794
Bpu10I CCTNAGC 1 cut(s) 1179
Bpu1102I GCTNAGC 1 cut(s) 194
BpuEI CTTGAG 5 cut(s) 118, 271, 461, 2201, 2239
BpuMI CCSGG 4 cut(s) 661, 993, 1581, 1582
BsaBI GATNNNNATC 1 cut(s) 1083
BsaI GGTCTC 2 cut(s) 167, 1218
BsaJI CCNNGG 3 cut(s) 660, 1580, 1581
BsaWI WCCGGW 1 cut(s) 2764
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 1672
Bse1I ACTGG 5 cut(s) 27, 312, 1438, 1666, 1798
Bse8I GATNNNNATC 1 cut(s) 1083
BseAI TCCGGA 1 cut(s) 2764
BseBI CCWGG 2 cut(s) 699, 1572
BseDI CCNNGG 3 cut(s) 660, 1580, 1581
BseJI GATNNNNATC 1 cut(s) 1083
BseLI CCNNNNNNNGG 2 cut(s) 291, 1672
BseMII CTCAG 5 cut(s) 208, 393, 1193, 1725, 1878
BseNI ACTGG 5 cut(s) 27, 312, 1438, 1666, 1798
BseRI GAGGAG 4 cut(s) 33, 215, 216, 1253
BseXI GCAGC 8 cut(s) 35, 268, 482, 497, 589, 1008, 2090, 2349
BseYI CCCAGC 1 cut(s) 2523
BsgI GTGCAG 2 cut(s) 588, 2356
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 4 cut(s) 505, 697, 991, 1185
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 3 cut(s) 567, 1580, 2507
BsiSI CCGG 4 cut(s) 660, 992, 1581, 2765
BslFI GGGAC 1 cut(s) 1932
BslI CCNNNNNNNGG 2 cut(s) 291, 1672
BsmAI GTCTC 9 cut(s) 167, 815, 978, 1218, 1287, 1770, 2504, 2626, 2743
BsmBI CGTCTC 1 cut(s) 978
BsmFI GGGAC 1 cut(s) 1932
BsmI GAATGC 3 cut(s) 329, 1702, 1769
BsnI GGCC 4 cut(s) 505, 697, 991, 1185
Bso31I GGTCTC 2 cut(s) 167, 1218
BsoBI CYCGRG 3 cut(s) 567, 1580, 2507
Bsp1286I GDGCHC 2 cut(s) 225, 1581
Bsp13I TCCGGA 1 cut(s) 2764
Bsp1720I GCTNAGC 1 cut(s) 194
BspACI CCGC 3 cut(s) 356, 2167, 2722
BspANI GGCC 4 cut(s) 505, 697, 991, 1185
BspCNI CTCAG 5 cut(s) 207, 392, 1192, 1724, 1879
BspEI TCCGGA 1 cut(s) 2764
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 4 cut(s) 375, 696, 2052, 2215
BspMI ACCTGC 1 cut(s) 2596
BspPI GGATC 6 cut(s) 144, 368, 381, 1268, 2238, 2307
BspQI GCTCTTC 1 cut(s) 61
BspTI CTTAAG 1 cut(s) 2303
BspTNI GGTCTC 2 cut(s) 167, 1218
BsrBI CCGCTC 2 cut(s) 356, 2169
BsrI ACTGG 5 cut(s) 27, 312, 1438, 1666, 1798
BssECI CCNNGG 3 cut(s) 660, 1580, 1581
BssNAI GTATAC 2 cut(s) 147, 1060
Bst1107I GTATAC 2 cut(s) 147, 1060
Bst2UI CCWGG 2 cut(s) 699, 1572
Bst4CI ACNGT 3 cut(s) 1388, 2334, 2678
Bst6I CTCTTC 1 cut(s) 61
BstAFI CTTAAG 1 cut(s) 2303
BstAPI GCANNNNNTGC 1 cut(s) 1163
BstC8I GCNNGC 4 cut(s) 1411, 2177, 2581, 2698
BstFNI CGCG 1 cut(s) 416
BstMAI GTCTC 9 cut(s) 167, 815, 978, 1218, 1287, 1770, 2504, 2626, 2743
BstMWI GCNNNNNNNGC 2 cut(s) 108, 1163
BstNI CCWGG 2 cut(s) 699, 1572
BstNSI RCATGY 1 cut(s) 1947
BstPAI GACNNNNGTC 1 cut(s) 1506
BstSCI CCNGG 6 cut(s) 659, 697, 991, 1570, 1579, 1580
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 8 cut(s) 35, 268, 482, 497, 589, 1008, 2090, 2349
BstV2I GAAGAC 5 cut(s) 85, 213, 256, 2564, 2681
BstX2I RGATCY 5 cut(s) 373, 788, 1273, 2230, 2299
BstYI RGATCY 5 cut(s) 373, 788, 1273, 2230, 2299
BstZ17I GTATAC 2 cut(s) 147, 1060
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 4 cut(s) 505, 697, 991, 1185
BtsIMutI CAGTG 1 cut(s) 1434
BveI ACCTGC 1 cut(s) 2596
Cac8I GCNNGC 4 cut(s) 1411, 2177, 2581, 2698
CaiI CAGNNNCTG 1 cut(s) 1433
Cfr13I GGNCC 4 cut(s) 695, 989, 1130, 1183
Cfr9I CCCGGG 1 cut(s) 1580
CseI GACGC 1 cut(s) 422
CsiI ACCWGGT 1 cut(s) 1570
Csp6I GTAC 3 cut(s) 386, 523, 1169
CviAII CATG 7 cut(s) 454, 585, 679, 1523, 1856, 1944, 1948
CviQI GTAC 3 cut(s) 386, 523, 1169
DraI TTTAAA 4 cut(s) 1335, 1369, 2143, 2361
DraIII CACNNNGTG 2 cut(s) 1163, 1574
DrdI GACNNNNNNGTC 1 cut(s) 829
DriI GACNNNNNGTC 1 cut(s) 638
DseDI GACNNNNNNGTC 1 cut(s) 829
Eam1104I CTCTTC 1 cut(s) 61
Eam1105I GACNNNNNGTC 1 cut(s) 638
EarI CTCTTC 1 cut(s) 61
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 2 cut(s) 225, 1581
Eco31I GGTCTC 2 cut(s) 167, 1218
Eco32I GATATC 1 cut(s) 1354
Eco47I GGWCC 1 cut(s) 1130
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 163
Eco88I CYCGRG 3 cut(s) 567, 1580, 2507
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 695, 1183
EcoRI GAATTC 2 cut(s) 1002, 2760
EcoRII CCWGG 2 cut(s) 697, 1570
EcoRV GATATC 1 cut(s) 1354
EcoT22I ATGCAT 2 cut(s) 1524, 1949
EcoT38I GRGCYC 2 cut(s) 225, 1581
Esp3I CGTCTC 1 cut(s) 978
FaeI CATG 7 cut(s) 457, 588, 682, 1526, 1859, 1947, 1951
FalI AAGNNNNNCTT 4 cut(s) 388, 420, 810, 842
FaqI GGGAC 1 cut(s) 1932
FatI CATG 7 cut(s) 453, 584, 678, 1522, 1855, 1943, 1947
FauI CCCGC 1 cut(s) 2715
FbaI TGATCA 4 cut(s) 64, 430, 871, 1084
FblI GTMKAC 5 cut(s) 146, 631, 1059, 1383, 1617
Fnu4HI GCNGC 8 cut(s) 24, 282, 471, 486, 603, 997, 2104, 2338
FriOI GRGCYC 2 cut(s) 225, 1581
Fsp4HI GCNGC 8 cut(s) 24, 282, 471, 486, 603, 997, 2104, 2338
FspBI CTAG 5 cut(s) 765, 806, 948, 1403, 2459
GluI GCNGC 8 cut(s) 24, 282, 471, 486, 603, 997, 2104, 2338
GsaI CCCAGC 1 cut(s) 2527
GsuI CTGGAG 2 cut(s) 1119, 1794
HaeIII GGCC 4 cut(s) 505, 697, 991, 1185
HapII CCGG 4 cut(s) 660, 992, 1581, 2765
HgaI GACGC 1 cut(s) 422
Hin1II CATG 7 cut(s) 457, 588, 682, 1526, 1859, 1947, 1951
HincII GTYRAC 4 cut(s) 643, 1618, 2098, 2599
HindII GTYRAC 4 cut(s) 643, 1618, 2098, 2599
HindIII AAGCTT 5 cut(s) 1089, 1407, 1458, 1705, 2177
HpaII CCGG 4 cut(s) 660, 992, 1581, 2765
HphI GGTGA 7 cut(s) 59, 422, 1385, 1560, 1586, 2578, 2695
HpyAV CCTTC 6 cut(s) 187, 460, 591, 850, 1196, 2471
HpyCH4III ACNGT 3 cut(s) 1388, 2334, 2678
HpyCH4IV ACGT 8 cut(s) 936, 1027, 1162, 1171, 1588, 1623, 1921, 2781
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 1163
HpySE526I ACGT 8 cut(s) 936, 1027, 1162, 1171, 1588, 1623, 1921, 2781
Hsp92II CATG 7 cut(s) 457, 588, 682, 1526, 1859, 1947, 1951
Kpn2I TCCGGA 1 cut(s) 2764
Ksp22I TGATCA 4 cut(s) 64, 430, 871, 1084
LguI GCTCTTC 1 cut(s) 61
LmnI GCTCC 3 cut(s) 228, 1602, 2767
Lsp1109I GCAGC 8 cut(s) 35, 268, 482, 497, 589, 1008, 2090, 2349
LweI GCATC 6 cut(s) 891, 1306, 1509, 1671, 2471, 2709
MabI ACCWGGT 1 cut(s) 1570
MaeI CTAG 5 cut(s) 765, 806, 948, 1403, 2459
MaeII ACGT 8 cut(s) 936, 1027, 1162, 1171, 1588, 1623, 1921, 2781
MaeIII GTNAC 6 cut(s) 410, 587, 1429, 1566, 1892, 2378
MbiI CCGCTC 2 cut(s) 356, 2169
MfeI CAATTG 2 cut(s) 234, 720
MflI RGATCY 5 cut(s) 373, 788, 1273, 2230, 2299
MhlI GDGCHC 2 cut(s) 225, 1581
MluI ACGCGT 1 cut(s) 414
MmeI TCCRAC 5 cut(s) 1466, 1818, 2044, 2277, 2429
Mph1103I ATGCAT 2 cut(s) 1524, 1949
MroI TCCGGA 1 cut(s) 2764
MslI CAYNNNNRTG 2 cut(s) 1517, 1909
MspCI CTTAAG 1 cut(s) 2303
MspI CCGG 4 cut(s) 660, 992, 1581, 2765
MspR9I CCNGG 6 cut(s) 661, 699, 993, 1572, 1581, 1582
MunI CAATTG 2 cut(s) 234, 720
Mva1269I GAATGC 3 cut(s) 329, 1702, 1769
MvaI CCWGG 2 cut(s) 699, 1572
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 2 cut(s) 108, 1163
NciI CCSGG 4 cut(s) 661, 993, 1581, 1582
NlaIII CATG 7 cut(s) 457, 588, 682, 1526, 1859, 1947, 1951
NlaIV GGNNCC 4 cut(s) 375, 696, 2052, 2215
NmuCI GTSAC 5 cut(s) 410, 587, 1429, 1566, 1892
NsiI ATGCAT 2 cut(s) 1524, 1949
NspI RCATGY 1 cut(s) 1947
PaeR7I CTCGAG 1 cut(s) 2507
PaqCI CACCTGC 1 cut(s) 2596
PciSI GCTCTTC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 1168
PctI GAATGC 3 cut(s) 329, 1702, 1769
PfeI GAWTC 9 cut(s) 332, 1393, 1723, 1899, 1998, 2020, 2057, 2067, 2825
PkrI GCNGC 8 cut(s) 25, 283, 472, 487, 604, 998, 2105, 2339
PshAI GACNNNNGTC 1 cut(s) 1506
Psp124BI GAGCTC 1 cut(s) 225
Psp1406I AACGTT 2 cut(s) 1027, 2781
Psp6I CCWGG 2 cut(s) 697, 1570
PspFI CCCAGC 1 cut(s) 2523
PspGI CCWGG 2 cut(s) 697, 1570
PspN4I GGNNCC 4 cut(s) 375, 696, 2052, 2215
PspPI GGNCC 4 cut(s) 695, 989, 1130, 1183
PstNI CAGNNNCTG 1 cut(s) 1433
PsuI RGATCY 5 cut(s) 373, 788, 1273, 2230, 2299
RsaI GTAC 3 cut(s) 387, 524, 1170
RsaNI GTAC 3 cut(s) 386, 523, 1169
RseI CAYNNNNRTG 2 cut(s) 1517, 1909
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 1616
SapI GCTCTTC 1 cut(s) 61
SatI GCNGC 8 cut(s) 24, 282, 471, 486, 603, 997, 2104, 2338
Sau96I GGNCC 4 cut(s) 695, 989, 1130, 1183
ScrFI CCNGG 6 cut(s) 661, 699, 993, 1572, 1581, 1582
SduI GDGCHC 2 cut(s) 225, 1581
SexAI ACCWGGT 1 cut(s) 1570
SfaNI GCATC 6 cut(s) 891, 1306, 1509, 1671, 2471, 2709
Sfr274I CTCGAG 1 cut(s) 2507
SinI GGWCC 1 cut(s) 1130
SlaI CTCGAG 1 cut(s) 2507
SmaI CCCGGG 1 cut(s) 1582
SmiMI CAYNNNNRTG 2 cut(s) 1517, 1909
SmlI CTYRAG 7 cut(s) 97, 286, 476, 2180, 2218, 2303, 2507
SmoI CTYRAG 7 cut(s) 97, 286, 476, 2180, 2218, 2303, 2507
SsiI CCGC 3 cut(s) 356, 2167, 2722
SspI AATATT 2 cut(s) 670, 1679
SspMI CTAG 5 cut(s) 765, 806, 948, 1403, 2459
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 6 cut(s) 659, 697, 991, 1570, 1579, 1580
TaaI ACNGT 3 cut(s) 1388, 2334, 2678
TaiI ACGT 8 cut(s) 939, 1030, 1165, 1174, 1591, 1626, 1924, 2784
TfiI GAWTC 9 cut(s) 332, 1393, 1723, 1899, 1998, 2020, 2057, 2067, 2825
TscAI CASTG 1 cut(s) 1434
TseFI GTSAC 5 cut(s) 410, 587, 1429, 1566, 1892
TseI GCWGC 8 cut(s) 23, 281, 470, 485, 602, 996, 2103, 2337
Tsp45I GTSAC 5 cut(s) 410, 587, 1429, 1566, 1892
TspGWI ACGGA 6 cut(s) 149, 960, 1491, 1818, 1911, 2050
TspMI CCCGGG 1 cut(s) 1580
TspRI CASTG 1 cut(s) 1434
Vha464I CTTAAG 1 cut(s) 2303
VpaK11BI GGWCC 1 cut(s) 1130
XceI RCATGY 1 cut(s) 1947
XcmI CCANNNNNNNNNTGG 2 cut(s) 2460, 2795
XhoI CTCGAG 1 cut(s) 2507
XmaI CCCGGG 1 cut(s) 1580
XmiI GTMKAC 5 cut(s) 146, 631, 1059, 1383, 1617
XspI CTAG 5 cut(s) 765, 806, 948, 1403, 2459
Zsp2I ATGCAT 2 cut(s) 1524, 1949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.