Prupe.1G076900_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016818
Physical Location & Seq
Reverse (-)
5563712 .. 5564835
1124 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G076900.1

Sequence Viewer

Length: 612 bp
ATGGGTGCTTGCTTTTCTTGCAAACCAAAACCTGCACTTCAAAATGTGAGAGTGGTTCATCTAAATGGCTATGTGGAGGACTATGAGCATCCAATTTCTGTAGGTCAAGTCACAGGGAAGCCCGCAAAGCACTTTGTCTGCACCCCAGCTCAGCTTCTCTCTACTGGGTCCAAGCCATTAAAACAGGACACCCTGCTGCAACCAGGGCAGGTCTATTTTCTGCTTCCATACTCAGCATTGCAGAGTGATGTTTCTCCTCTGGACTGGGCCTCCATATCCAGAAAGCTCACTGCAGCAGCAGCAAAAAGCAGGAGGTCTTCATGTGAGGCTAACAATAATTCTTCTCATTCTTCTCCTTTGGCTCCTTCTAGTCCTTTGATGATGGGCCAGTTTTGCTCAAGCCCTGTGTGGAGCTCATCATCTTCACCAGGTAGAAGTCCTAGCAGGTTTTCTATGGAGCAGGAAAACATGGGCTCTCTGATGGATTATGGCTCTCAAAGGTCTTGTAGAGCTAGGTCATGGAAGCCACTTTTGGATACAATTACTGAGAAATCATTTAACAGGAGGACTGAATCAGATTTACAAGAGATGCATGTGGAGGCTGTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

204

Amino Acids

22.26

Weight (kDa)

9.08

Isoelectric Point (pI)

74.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016818)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07400
malus_domestica MD13G1243500.v1.1 MD16G1250000.v1.1
prunus_persica Prupe.1G076900_v2.0.a1
pyrus_communis pycom13g21490
rosa_chinensis RchiOBHm_Chr4g0399841
rosa_laevigata RLG00000009238
rosa_multiflora Rmu_sc0014676.1_g000014
rosa_roxburghii Rroxscaffold_5G00344730
rosa_rugosa Rorug04G0019400
rosa_samantha Rh4BG094100 Rh4CG105800 Rh4DG090700
rosa_wichuraiana Rw4G007990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 40, 199, 435
AciI CCGC 1 cut(s) 123
AgsI TTSAA 1 cut(s) 41
AjnI CCWGG 2 cut(s) 202, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 515, 547
AluBI AGCT 5 cut(s) 149, 154, 286, 414, 512
AluI AGCT 5 cut(s) 149, 154, 286, 414, 512
Alw21I GWGCWC 1 cut(s) 416
AoxI GGCC 2 cut(s) 267, 385
ApeKI GCWGC 4 cut(s) 196, 293, 296, 299
AspS9I GGNCC 3 cut(s) 168, 267, 385
AsuHPI GGTGA 1 cut(s) 417
AvaII GGWCC 1 cut(s) 168
BanII GRGCYC 2 cut(s) 416, 476
BbsI GAAGAC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 416
BbvI GCAGC 4 cut(s) 183, 305, 308, 311
BccI CCATC 2 cut(s) 376, 475
BciT130I CCWGG 2 cut(s) 204, 429
BciVI GTATCC 1 cut(s) 529
BfaI CTAG 3 cut(s) 369, 441, 513
BfmI CTRYAG 2 cut(s) 99, 291
BfuAI ACCTGC 3 cut(s) 40, 199, 435
BfuI GTATCC 1 cut(s) 529
BisI GCNGC 4 cut(s) 197, 294, 297, 300
BlpI GCTNAGC 1 cut(s) 150
BlsI GCNGC 4 cut(s) 198, 295, 298, 301
Bme1390I CCNGG 2 cut(s) 204, 429
Bme18I GGWCC 1 cut(s) 168
BmgT120I GGNCC 3 cut(s) 168, 267, 385
BmiI GGNNCC 2 cut(s) 169, 363
BmrFI CCNGG 2 cut(s) 204, 429
BmrI ACTGGG 2 cut(s) 174, 274
BmsI GCATC 2 cut(s) 97, 579
BmuI ACTGGG 2 cut(s) 174, 274
BpiI GAAGAC 1 cut(s) 309
Bpu1102I GCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 382
BsaJI CCNNGG 1 cut(s) 203
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bse1I ACTGG 3 cut(s) 169, 269, 388
Bse3DI GCAATG 1 cut(s) 236
BseBI CCWGG 2 cut(s) 204, 429
BseDI CCNNGG 1 cut(s) 203
BseGI GGATG 1 cut(s) 88
BseMI GCAATG 1 cut(s) 236
BseMII CTCAG 3 cut(s) 164, 246, 537
BseNI ACTGG 3 cut(s) 169, 269, 388
BseRI GAGGAG 1 cut(s) 246
BseXI GCAGC 4 cut(s) 183, 305, 308, 311
BseYI CCCAGC 1 cut(s) 145
BsgI GTGCAG 2 cut(s) 18, 124
BshFI GGCC 2 cut(s) 269, 387
BsiHKAI GWGCWC 1 cut(s) 416
BsnI GGCC 2 cut(s) 269, 387
Bsp1286I GDGCHC 2 cut(s) 416, 476
Bsp1720I GCTNAGC 1 cut(s) 150
BspACI CCGC 1 cut(s) 123
BspANI GGCC 2 cut(s) 269, 387
BspCNI CTCAG 3 cut(s) 163, 245, 538
BspLI GGNNCC 2 cut(s) 169, 363
BspMAI CTGCAG 1 cut(s) 295
BspMI ACCTGC 3 cut(s) 40, 199, 435
BsrDI GCAATG 1 cut(s) 236
BsrI ACTGG 3 cut(s) 169, 269, 388
BssECI CCNNGG 1 cut(s) 203
Bst2UI CCWGG 2 cut(s) 204, 429
BstC8I GCNNGC 2 cut(s) 10, 123
BstDEI CTNAG 3 cut(s) 150, 232, 546
BstF5I GGATG 1 cut(s) 88
BstMWI GCNNNNNNNGC 5 cut(s) 18, 127, 205, 299, 393
BstNI CCWGG 2 cut(s) 204, 429
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 2 cut(s) 202, 427
BstSFI CTRYAG 2 cut(s) 99, 291
BstV1I GCAGC 4 cut(s) 183, 305, 308, 311
BstV2I GAAGAC 1 cut(s) 309
BsuI GTATCC 1 cut(s) 529
BsuRI GGCC 2 cut(s) 269, 387
BtsCI GGATG 1 cut(s) 88
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 288
BveI ACCTGC 3 cut(s) 40, 199, 435
Cac8I GCNNGC 2 cut(s) 10, 123
Cfr13I GGNCC 3 cut(s) 168, 267, 385
CsiI ACCWGGT 1 cut(s) 427
CviAII CATG 4 cut(s) 321, 469, 519, 593
DdeI CTNAG 3 cut(s) 150, 232, 546
Ecl136II GAGCTC 1 cut(s) 414
Eco24I GRGCYC 2 cut(s) 416, 476
Eco47I GGWCC 1 cut(s) 168
Eco53kI GAGCTC 1 cut(s) 414
EcoICRI GAGCTC 1 cut(s) 414
EcoRII CCWGG 2 cut(s) 202, 427
EcoT22I ATGCAT 1 cut(s) 594
EcoT38I GRGCYC 2 cut(s) 416, 476
FaeI CATG 4 cut(s) 324, 472, 522, 596
FatI CATG 4 cut(s) 320, 468, 518, 592
FauI CCCGC 1 cut(s) 130
Fnu4HI GCNGC 4 cut(s) 197, 294, 297, 300
FokI GGATG 1 cut(s) 75
FriOI GRGCYC 2 cut(s) 416, 476
Fsp4HI GCNGC 4 cut(s) 197, 294, 297, 300
FspBI CTAG 3 cut(s) 369, 441, 513
GluI GCNGC 4 cut(s) 197, 294, 297, 300
GsaI CCCAGC 1 cut(s) 149
HaeIII GGCC 2 cut(s) 269, 387
Hin1II CATG 4 cut(s) 324, 472, 522, 596
HinfI GANTC 1 cut(s) 572
HphI GGTGA 1 cut(s) 417
Hpy188I TCNGA 2 cut(s) 480, 577
Hpy188III TCNNGA 2 cut(s) 260, 279
HpyAV CCTTC 1 cut(s) 375
HpyCH4V TGCA 7 cut(s) 21, 35, 141, 199, 241, 293, 592
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 127, 205, 299, 393
HpyF3I CTNAG 3 cut(s) 150, 232, 546
Hsp92II CATG 4 cut(s) 324, 472, 522, 596
LmnI GCTCC 3 cut(s) 367, 411, 457
Lsp1109I GCAGC 4 cut(s) 183, 305, 308, 311
LweI GCATC 2 cut(s) 97, 579
MabI ACCWGGT 1 cut(s) 427
MaeI CTAG 3 cut(s) 369, 441, 513
MaeIII GTNAC 1 cut(s) 109
MboII GAAGA 4 cut(s) 309, 333, 342, 414
MhlI GDGCHC 2 cut(s) 416, 476
MluCI AATT 3 cut(s) 93, 337, 540
MnlI CCTC 7 cut(s) 70, 267, 280, 306, 319, 558, 592
Mph1103I ATGCAT 1 cut(s) 594
MseI TTAA 2 cut(s) 179, 558
MslI CAYNNNNRTG 1 cut(s) 63
MspR9I CCNGG 2 cut(s) 204, 429
MvaI CCWGG 2 cut(s) 204, 429
MwoI GCNNNNNNNGC 5 cut(s) 18, 127, 205, 299, 393
NlaIII CATG 4 cut(s) 324, 472, 522, 596
NlaIV GGNNCC 2 cut(s) 169, 363
NmuCI GTSAC 1 cut(s) 109
NsiI ATGCAT 1 cut(s) 594
NspI RCATGY 1 cut(s) 596
PfeI GAWTC 1 cut(s) 572
PkrI GCNGC 4 cut(s) 198, 295, 298, 301
Psp124BI GAGCTC 1 cut(s) 416
Psp6I CCWGG 2 cut(s) 202, 427
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 2 cut(s) 202, 427
PspN4I GGNNCC 2 cut(s) 169, 363
PspPI GGNCC 3 cut(s) 168, 267, 385
PstI CTGCAG 1 cut(s) 295
RseI CAYNNNNRTG 1 cut(s) 63
SacI GAGCTC 1 cut(s) 416
SaqAI TTAA 2 cut(s) 179, 558
SatI GCNGC 4 cut(s) 197, 294, 297, 300
Sau96I GGNCC 3 cut(s) 168, 267, 385
ScrFI CCNGG 2 cut(s) 204, 429
SduI GDGCHC 2 cut(s) 416, 476
SexAI ACCWGGT 1 cut(s) 427
SfaNI GCATC 2 cut(s) 97, 579
SfcI CTRYAG 2 cut(s) 99, 291
SinI GGWCC 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 397
SmoI CTYRAG 1 cut(s) 397
Sse9I AATT 3 cut(s) 93, 337, 540
SsiI CCGC 1 cut(s) 123
SspMI CTAG 3 cut(s) 369, 441, 513
SstI GAGCTC 1 cut(s) 416
StyD4I CCNGG 2 cut(s) 202, 427
TasI AATT 3 cut(s) 93, 337, 540
TfiI GAWTC 1 cut(s) 572
Tru1I TTAA 2 cut(s) 179, 558
Tru9I TTAA 2 cut(s) 179, 558
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 4 cut(s) 196, 293, 296, 299
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 2 cut(s) 47, 309
TspRI CASTG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 168
XceI RCATGY 1 cut(s) 596
XspI CTAG 3 cut(s) 369, 441, 513
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.