Rroxscaffold_5G00344730

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016818
Physical Location & Seq
Reverse (-)
14855883 .. 14858187
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00344730.1

Sequence Viewer

Length: 570 bp
ATGCGAGGCGGCGAGACCTTCGGCATAGGCGTAGTCCAAGGAAGATGGATGGTCGTTGGATTTTGGGGTGCGCAGATCGATCTCAATCGGTCCATTCGGATCTTTGGTGAACGCGGACCAAAGCCAGAAGAAACCCAGAACAATGTGCGCGTGGTTCATCTCAACGGCTACGTCGAAGAGTTCGAGCACCCAGTTTCGAGTGATGCTTCTCCTATGGACTTGGCTGCCGTGGTCCGGAAGCTAACGGCTTTGGCAAGAAGTGGTAGTAGTAGGAGTAATGAGGGGTTGGGAAGGTCTGGTAGTTCGTCGAGGTCCGGTCAGATGAGTCCTATGCATTGCTCTAGTCCTGTTTGGAGTAACTATTCATCATCGCCGGGGAGAAATAGTCCAGGCCGGTTTGTGGAGCCGGAGGGTAGTCCTAGTCGGATGGAGATGTATGGTGCGCAGAGGTCTACAAGAGCAAGGTCATGGAAGCCACTGTTGGATACTATAACGGAGAGGCAGTTGAACCGGAGAACGGAGTCGGATTTGCAGGAGAAGCATGTGGAGGCAGCCAAATATCTACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

189

Amino Acids

20.97

Weight (kDa)

9.71

Isoelectric Point (pI)

74.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016818)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07400
malus_domestica MD13G1243500.v1.1 MD16G1250000.v1.1
prunus_persica Prupe.1G076900_v2.0.a1
pyrus_communis pycom13g21490
rosa_chinensis RchiOBHm_Chr4g0399841
rosa_laevigata RLG00000009238
rosa_multiflora Rmu_sc0014676.1_g000014
rosa_roxburghii Rroxscaffold_5G00344730
rosa_rugosa Rorug04G0019400
rosa_samantha Rh4BG094100 Rh4CG105800 Rh4DG090700
rosa_wichuraiana Rw4G007990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 72, 444
AccI GTMKAC 1 cut(s) 452
AccII CGCG 2 cut(s) 114, 150
AccIII TCCGGA 1 cut(s) 234
AciI CCGC 2 cut(s) 9, 114
AclWI GGATC 1 cut(s) 107
AfiI CCNNNNNNNGG 3 cut(s) 234, 400, 517
AgsI TTSAA 1 cut(s) 508
AjnI CCWGG 1 cut(s) 388
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
AluBI AGCT 1 cut(s) 241
AluI AGCT 1 cut(s) 241
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 1 cut(s) 107
Aor13HI TCCGGA 1 cut(s) 234
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 2 cut(s) 224, 551
AspLEI GCGC 3 cut(s) 73, 150, 445
AspS9I GGNCC 4 cut(s) 90, 116, 232, 312
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 119
AvaII GGWCC 4 cut(s) 90, 116, 232, 312
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 211, 563
BccI CCATC 3 cut(s) 39, 43, 421
BceAI ACGGC 3 cut(s) 181, 212, 261
BciT130I CCWGG 1 cut(s) 390
BciVI GTATCC 1 cut(s) 478
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 2 cut(s) 342, 420
BfmI CTRYAG 1 cut(s) 563
BfuI GTATCC 1 cut(s) 478
BisI GCNGC 3 cut(s) 10, 225, 552
BlsI GCNGC 3 cut(s) 11, 226, 553
Bme1390I CCNGG 2 cut(s) 375, 390
Bme18I GGWCC 4 cut(s) 90, 116, 232, 312
BmgT120I GGNCC 4 cut(s) 90, 116, 232, 312
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 2 cut(s) 375, 390
BmrI ACTGGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 193
BmuI ACTGGG 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 37, 228, 374
BsaWI WCCGGW 3 cut(s) 234, 314, 510
BsaXI ACNNNNNCTCC 4 cut(s) 370, 400, 488, 518
Bsc4I CCNNNNNNNGG 3 cut(s) 234, 400, 517
Bse118I RCCGGY 1 cut(s) 393
Bse1I ACTGG 1 cut(s) 191
Bse3DI GCAATG 1 cut(s) 334
Bse8I GATNNNNATC 1 cut(s) 84
BseAI TCCGGA 1 cut(s) 234
BseBI CCWGG 1 cut(s) 390
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 3 cut(s) 37, 228, 374
BseGI GGATG 2 cut(s) 54, 432
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 3 cut(s) 234, 400, 517
BseMI GCAATG 1 cut(s) 334
BseNI ACTGG 1 cut(s) 191
BseXI GCAGC 2 cut(s) 211, 563
Bsh1236I CGCG 2 cut(s) 114, 150
BshFI GGCC 1 cut(s) 393
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 6 cut(s) 235, 315, 374, 394, 407, 511
BslI CCNNNNNNNGG 3 cut(s) 234, 400, 517
BsmAI GTCTC 1 cut(s) 8
BsnI GGCC 1 cut(s) 393
Bso31I GGTCTC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 189
Bsp13I TCCGGA 1 cut(s) 234
Bsp143I GATC 3 cut(s) 75, 79, 99
BspACI CCGC 2 cut(s) 9, 114
BspANI GGCC 1 cut(s) 393
BspDI ATCGAT 1 cut(s) 78
BspEI TCCGGA 1 cut(s) 234
BspFNI CGCG 2 cut(s) 114, 150
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 393
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 393
BssECI CCNNGG 3 cut(s) 37, 228, 374
BssMI GATC 3 cut(s) 75, 79, 99
BssT1I CCWWGG 1 cut(s) 37
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 2 cut(s) 480, 567
Bst6I CTCTTC 1 cut(s) 171
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 2 cut(s) 54, 432
BstFNI CGCG 2 cut(s) 114, 150
BstHHI GCGC 3 cut(s) 73, 150, 445
BstKTI GATC 3 cut(s) 78, 82, 102
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 3 cut(s) 75, 79, 99
BstMWI GCNNNNNNNGC 1 cut(s) 538
BstNI CCWGG 1 cut(s) 390
BstNSI RCATGY 1 cut(s) 545
BstSCI CCNGG 2 cut(s) 373, 388
BstSFI CTRYAG 1 cut(s) 563
BstUI CGCG 2 cut(s) 114, 150
BstV1I GCAGC 2 cut(s) 211, 563
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
Bsu15I ATCGAT 1 cut(s) 78
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 1 cut(s) 393
BsuTUI ATCGAT 1 cut(s) 78
BtgI CCRYGG 1 cut(s) 228
BtgZI GCGATG 1 cut(s) 354
BtsCI GGATG 2 cut(s) 54, 432
BtsIMutI CAGTG 1 cut(s) 476
CfoI GCGC 3 cut(s) 73, 150, 445
Cfr10I RCCGGY 1 cut(s) 393
Cfr13I GGNCC 4 cut(s) 90, 116, 232, 312
ClaI ATCGAT 1 cut(s) 78
CviAII CATG 2 cut(s) 468, 542
CviJI RGCY 9 cut(s) 124, 168, 224, 241, 248, 393, 406, 475, 554
CviKI_1 RGCY 9 cut(s) 124, 168, 224, 241, 248, 393, 406, 475, 554
DpnI GATC 3 cut(s) 77, 81, 101
DpnII GATC 3 cut(s) 75, 79, 99
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 37
Eco31I GGTCTC 1 cut(s) 8
Eco47I GGWCC 4 cut(s) 90, 116, 232, 312
EcoRII CCWGG 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 37
EcoT22I ATGCAT 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 2 cut(s) 471, 545
FaiI YATR 7 cut(s) 26, 215, 332, 438, 469, 491, 543
FatI CATG 2 cut(s) 467, 541
FblI GTMKAC 1 cut(s) 452
Fnu4HI GCNGC 3 cut(s) 10, 225, 552
FokI GGATG 2 cut(s) 61, 439
Fsp4HI GCNGC 3 cut(s) 10, 225, 552
FspBI CTAG 2 cut(s) 342, 420
FspI TGCGCA 2 cut(s) 72, 444
GlaI GCGC 3 cut(s) 72, 149, 444
GluI GCNGC 3 cut(s) 10, 225, 552
HaeIII GGCC 1 cut(s) 393
HapII CCGG 6 cut(s) 235, 315, 374, 394, 407, 511
HhaI GCGC 3 cut(s) 73, 150, 445
Hin1II CATG 2 cut(s) 471, 545
Hin6I GCGC 3 cut(s) 71, 148, 443
HinP1I GCGC 3 cut(s) 71, 148, 443
HinfI GANTC 2 cut(s) 325, 521
HpaII CCGG 6 cut(s) 235, 315, 374, 394, 407, 511
HphI GGTGA 1 cut(s) 119
Hpy166II GTNNAC 2 cut(s) 110, 453
Hpy188I TCNGA 4 cut(s) 99, 321, 426, 526
Hpy188III TCNNGA 1 cut(s) 235
Hpy8I GTNNAC 2 cut(s) 110, 453
Hpy99I CGWCG 2 cut(s) 176, 310
HpyAV CCTTC 2 cut(s) 28, 285
HpyCH4III ACNGT 2 cut(s) 480, 567
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 2 cut(s) 334, 532
HpyF10VI GCNNNNNNNGC 1 cut(s) 538
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 2 cut(s) 471, 545
HspAI GCGC 3 cut(s) 71, 148, 443
Kpn2I TCCGGA 1 cut(s) 234
Kzo9I GATC 3 cut(s) 75, 79, 99
LmnI GCTCC 1 cut(s) 403
Lsp1109I GCAGC 2 cut(s) 211, 563
LweI GCATC 1 cut(s) 193
MaeI CTAG 2 cut(s) 342, 420
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 356
MalI GATC 3 cut(s) 77, 81, 101
MboI GATC 3 cut(s) 75, 79, 99
MboII GAAGA 3 cut(s) 54, 140, 188
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 1 cut(s) 189
MlyI GAGTC 2 cut(s) 334, 530
MmeI TCCRAC 4 cut(s) 37, 404, 462, 504
MnlI CCTC 6 cut(s) 274, 303, 403, 441, 492, 541
Mph1103I ATGCAT 1 cut(s) 336
MroI TCCGGA 1 cut(s) 234
MspI CCGG 6 cut(s) 235, 315, 374, 394, 407, 511
MspR9I CCNGG 2 cut(s) 375, 390
MvaI CCWGG 1 cut(s) 390
MvnI CGCG 2 cut(s) 114, 150
MwoI GCNNNNNNNGC 1 cut(s) 538
NciI CCSGG 1 cut(s) 375
NdeII GATC 3 cut(s) 75, 79, 99
NlaIII CATG 2 cut(s) 471, 545
NlaIV GGNNCC 1 cut(s) 405
NsbI TGCGCA 2 cut(s) 72, 444
NsiI ATGCAT 1 cut(s) 336
NspI RCATGY 1 cut(s) 545
PcsI WCGNNNNNNNCGW 3 cut(s) 27, 171, 180
PkrI GCNGC 3 cut(s) 11, 226, 553
PleI GAGTC 2 cut(s) 333, 529
PpsI GAGTC 2 cut(s) 333, 529
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 4 cut(s) 90, 116, 232, 312
PsuI RGATCY 1 cut(s) 99
SatI GCNGC 3 cut(s) 10, 225, 552
Sau3AI GATC 3 cut(s) 75, 79, 99
Sau96I GGNCC 4 cut(s) 90, 116, 232, 312
SchI GAGTC 2 cut(s) 334, 530
ScrFI CCNGG 2 cut(s) 375, 390
SduI GDGCHC 1 cut(s) 189
SetI ASST 7 cut(s) 20, 174, 243, 296, 314, 452, 467
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 1 cut(s) 563
SinI GGWCC 4 cut(s) 90, 116, 232, 312
SsiI CCGC 2 cut(s) 9, 114
SspMI CTAG 2 cut(s) 342, 420
StyD4I CCNGG 2 cut(s) 373, 388
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 2 cut(s) 480, 567
TaiI ACGT 1 cut(s) 174
TaqI TCGA 5 cut(s) 78, 174, 183, 197, 308
TaqII GACCGA 1 cut(s) 78
TauI GCSGC 1 cut(s) 12
TscAI CASTG 1 cut(s) 483
TseI GCWGC 2 cut(s) 224, 551
TspDTI ATGAA 2 cut(s) 146, 354
TspGWI ACGGA 2 cut(s) 509, 533
TspRI CASTG 1 cut(s) 483
VpaK11BI GGWCC 4 cut(s) 90, 116, 232, 312
XceI RCATGY 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 452
XspI CTAG 2 cut(s) 342, 420
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.