MD13G1243500.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016818
Physical Location & Seq
Reverse (-)
25399327 .. 25399932
606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1243500.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGGTGGTTGCTTTTCTTGCAGACCAAAAGTTGAACTCCAGAATGTGAAGGTGGTGCATCTGAATGGGTATGTGGAGGATTTTGAGCACCCAATCTCAGTAGGGCAAGTGACAGGGAAGCCCACAAAGCACTTTGTATGCACTCCATCGCAGCTCCTCTCAACTGGGTCCAAGCCATTGAAGCATGACACCCTCCTCCAACCAGGGCAGGTCTACTTCCTCCTTCCCTACTCTGCACTGCGGAGTGATGCTTCTCCTCTCGACTTGGCTTCCATAGCCAGAAAACTCACTTCTGCAGCAGCAAAGAGCAGCAGGAAGCAGTCTCCGTCTGCCCCTCAGTCTCCTGCGGCCTATTCTTCAAGCATGAGCGGCCAGTTTTGCCCAAGCCCTATGTGGAGCTCTTCGTCGTCGCCGGGGAGGAGTCCAGGGAGGAGTCCGGGAAGGTTTGTGGAGCAGGAGAATTTGAGTATGATGGTGGATTATGGCTCTCAGAGATCGTCGCGTCGTTCGTGGAAGCCGCTGTTGGATACGATAACGGAGAGATCGTTGAACAAGATGACTGAGTCTGATGTGCAGGAGAGGCATGTGGAGGTGCTTGTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

202

Amino Acids

22.0

Weight (kDa)

9.48

Isoelectric Point (pI)

75.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 180 1e-31 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016818)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07400
malus_domestica MD13G1243500.v1.1 MD16G1250000.v1.1
prunus_persica Prupe.1G076900_v2.0.a1
pyrus_communis pycom13g21490
rosa_chinensis RchiOBHm_Chr4g0399841
rosa_laevigata RLG00000009238
rosa_multiflora Rmu_sc0014676.1_g000014
rosa_roxburghii Rroxscaffold_5G00344730
rosa_rugosa Rorug04G0019400
rosa_samantha Rh4BG094100 Rh4CG105800 Rh4DG090700
rosa_wichuraiana Rw4G007990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
AccBSI CCGCTC 1 cut(s) 369
AccI GTMKAC 1 cut(s) 213
AccII CGCG 1 cut(s) 502
AciI CCGC 4 cut(s) 241, 347, 369, 518
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 1 cut(s) 460
AgsI TTSAA 4 cut(s) 35, 181, 360, 550
AjnI CCWGG 2 cut(s) 202, 424
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 2 cut(s) 154, 399
AluI AGCT 2 cut(s) 154, 399
Alw21I GWGCWC 2 cut(s) 90, 401
Alw26I GTCTC 2 cut(s) 327, 345
AoxI GGCC 2 cut(s) 348, 370
ApeKI GCWGC 4 cut(s) 151, 296, 299, 309
ApoI RAATTY 1 cut(s) 460
AspS9I GGNCC 1 cut(s) 168
AsuC2I CCSGG 2 cut(s) 414, 438
AvaII GGWCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 401
Bbv12I GWGCWC 2 cut(s) 90, 401
BbvI GCAGC 4 cut(s) 163, 308, 311, 321
BccI CCATC 2 cut(s) 154, 466
BciT130I CCWGG 2 cut(s) 204, 426
BciVI GTATCC 1 cut(s) 520
BcnI CCSGG 2 cut(s) 414, 438
BcoDI GTCTC 2 cut(s) 327, 345
BfmI CTRYAG 1 cut(s) 294
BfuAI ACCTGC 1 cut(s) 199
BfuI GTATCC 1 cut(s) 520
BisI GCNGC 7 cut(s) 152, 297, 300, 310, 348, 370, 518
BlsI GCNGC 7 cut(s) 153, 298, 301, 311, 349, 371, 519
Bme1390I CCNGG 4 cut(s) 204, 414, 426, 438
Bme18I GGWCC 1 cut(s) 168
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 4 cut(s) 204, 414, 426, 438
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 67, 238
BmuI ACTGGG 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 23
BpuMI CCSGG 2 cut(s) 414, 438
BsaJI CCNNGG 3 cut(s) 203, 413, 425
BsaXI ACNNNNNCTCC 2 cut(s) 530, 560
Bse1I ACTGG 2 cut(s) 169, 373
BseBI CCWGG 2 cut(s) 204, 426
BseDI CCNNGG 3 cut(s) 203, 413, 425
BseMII CTCAG 4 cut(s) 111, 350, 503, 552
BseNI ACTGG 2 cut(s) 169, 373
BseRI GAGGAG 5 cut(s) 146, 185, 246, 433, 445
BseXI GCAGC 4 cut(s) 163, 308, 311, 321
BsgI GTGCAG 2 cut(s) 219, 593
Bsh1236I CGCG 1 cut(s) 502
BshFI GGCC 2 cut(s) 350, 372
BsiHKAI GWGCWC 2 cut(s) 90, 401
BsiSI CCGG 2 cut(s) 413, 437
BsmAI GTCTC 2 cut(s) 327, 345
BsnI GGCC 2 cut(s) 350, 372
Bsp1286I GDGCHC 2 cut(s) 90, 401
Bsp143I GATC 2 cut(s) 494, 542
BspACI CCGC 4 cut(s) 241, 347, 369, 518
BspANI GGCC 2 cut(s) 350, 372
BspCNI CTCAG 4 cut(s) 110, 349, 502, 553
BspFNI CGCG 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 169
BspMAI CTGCAG 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 199
BspQI GCTCTTC 1 cut(s) 406
BsrBI CCGCTC 1 cut(s) 369
BsrI ACTGG 2 cut(s) 169, 373
BssECI CCNNGG 3 cut(s) 203, 413, 425
BssMI GATC 2 cut(s) 494, 542
Bst2UI CCWGG 2 cut(s) 204, 426
Bst6I CTCTTC 1 cut(s) 406
BstDEI CTNAG 4 cut(s) 97, 336, 489, 561
BstFNI CGCG 1 cut(s) 502
BstKTI GATC 2 cut(s) 497, 545
BstMAI GTCTC 2 cut(s) 327, 345
BstMBI GATC 2 cut(s) 494, 542
BstMWI GCNNNNNNNGC 7 cut(s) 18, 127, 181, 275, 369, 378, 580
BstNI CCWGG 2 cut(s) 204, 426
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 4 cut(s) 202, 412, 424, 436
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 502
BstV1I GCAGC 4 cut(s) 163, 308, 311, 321
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 2 cut(s) 350, 372
BtgZI GCGATG 1 cut(s) 132
BtsI GCAGTG 1 cut(s) 236
BtsIMutI CAGTG 1 cut(s) 236
BveI ACCTGC 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 168
CseI GACGC 1 cut(s) 491
CviAII CATG 3 cut(s) 185, 364, 584
DdeI CTNAG 4 cut(s) 97, 336, 489, 561
DpnI GATC 2 cut(s) 496, 544
DpnII GATC 2 cut(s) 494, 542
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 406
EarI CTCTTC 1 cut(s) 406
Ecl136II GAGCTC 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 168
Eco53kI GAGCTC 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 399
EcoRII CCWGG 2 cut(s) 202, 424
EcoT38I GRGCYC 1 cut(s) 401
FaeI CATG 3 cut(s) 188, 367, 587
FaiI YATR 9 cut(s) 72, 139, 186, 275, 365, 392, 470, 483, 585
FatI CATG 3 cut(s) 184, 363, 583
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 7 cut(s) 152, 297, 300, 310, 348, 370, 518
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 7 cut(s) 152, 297, 300, 310, 348, 370, 518
GluI GCNGC 7 cut(s) 152, 297, 300, 310, 348, 370, 518
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 2 cut(s) 350, 372
HapII CCGG 2 cut(s) 413, 437
HgaI GACGC 1 cut(s) 491
Hin1II CATG 3 cut(s) 188, 367, 587
HinfI GANTC 3 cut(s) 421, 433, 563
HpaII CCGG 2 cut(s) 413, 437
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 3 cut(s) 63, 492, 568
Hpy188III TCNNGA 2 cut(s) 40, 260
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 4 cut(s) 409, 412, 502, 507
HpyAV CCTTC 3 cut(s) 43, 233, 435
HpyCH4V TGCA 6 cut(s) 21, 58, 141, 236, 296, 574
HpyF10VI GCNNNNNNNGC 7 cut(s) 18, 127, 181, 275, 369, 378, 580
HpyF3I CTNAG 4 cut(s) 97, 336, 489, 561
Hsp92II CATG 3 cut(s) 188, 367, 587
Kzo9I GATC 2 cut(s) 494, 542
LguI GCTCTTC 1 cut(s) 406
LmnI GCTCC 3 cut(s) 159, 396, 451
Lsp1109I GCAGC 4 cut(s) 163, 308, 311, 321
LweI GCATC 2 cut(s) 67, 238
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 496, 544
MbiI CCGCTC 1 cut(s) 369
MboI GATC 2 cut(s) 494, 542
MboII GAAGA 2 cut(s) 348, 393
MhlI GDGCHC 2 cut(s) 90, 401
MluCI AATT 1 cut(s) 460
MlyI GAGTC 3 cut(s) 430, 442, 572
MmeI TCCRAC 2 cut(s) 223, 504
MslI CAYNNNNRTG 1 cut(s) 63
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 2 cut(s) 413, 437
MspR9I CCNGG 4 cut(s) 204, 414, 426, 438
MvaI CCWGG 2 cut(s) 204, 426
MvnI CGCG 1 cut(s) 502
MwoI GCNNNNNNNGC 7 cut(s) 18, 127, 181, 275, 369, 378, 580
NciI CCSGG 2 cut(s) 414, 438
NdeII GATC 2 cut(s) 494, 542
NlaIII CATG 3 cut(s) 188, 367, 587
NlaIV GGNNCC 1 cut(s) 169
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 587
PciSI GCTCTTC 1 cut(s) 406
PcsI WCGNNNNNNNCGW 2 cut(s) 506, 542
PflFI GACNNNGTC 1 cut(s) 562
PfoI TCCNGGA 1 cut(s) 436
PkrI GCNGC 7 cut(s) 153, 298, 301, 311, 349, 371, 519
PleI GAGTC 3 cut(s) 429, 441, 571
PpsI GAGTC 3 cut(s) 429, 441, 571
Psp124BI GAGCTC 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 202, 424
PspGI CCWGG 2 cut(s) 202, 424
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 1 cut(s) 168
PstI CTGCAG 1 cut(s) 298
PsyI GACNNNGTC 1 cut(s) 562
RseI CAYNNNNRTG 1 cut(s) 63
SacI GAGCTC 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 406
SatI GCNGC 7 cut(s) 152, 297, 300, 310, 348, 370, 518
Sau3AI GATC 2 cut(s) 494, 542
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 3 cut(s) 430, 442, 572
ScrFI CCNGG 4 cut(s) 204, 414, 426, 438
SduI GDGCHC 2 cut(s) 90, 401
SetI ASST 6 cut(s) 54, 156, 213, 401, 446, 594
SfaNI GCATC 2 cut(s) 67, 238
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 63
Sse9I AATT 1 cut(s) 460
SsiI CCGC 4 cut(s) 241, 347, 369, 518
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 4 cut(s) 202, 412, 424, 436
TaqI TCGA 1 cut(s) 261
TasI AATT 1 cut(s) 460
TauI GCSGC 3 cut(s) 350, 372, 520
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 4 cut(s) 151, 296, 299, 309
Tsp45I GTSAC 1 cut(s) 109
TspGWI ACGGA 2 cut(s) 315, 551
TspRI CASTG 1 cut(s) 243
Tth111I GACNNNGTC 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 168
XapI RAATTY 1 cut(s) 460
XceI RCATGY 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 390
XmiI GTMKAC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.