FvH4_4g09151

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018830
Physical Location & Seq
Reverse (-)
10556367 .. 10557622
1256 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g09151.t1

Sequence Viewer

Length: 303 bp
ATGTCTTCTTCCACTACTTCAGTAGTGTTGCTGATCATTCCTCTACTTGTAGTTAGCTCTTTCATAGTTGCCTCTGCTGGTAACTTCAACCAAGACTTCAAGATTACATGGGGCGACGGTCGAGCCAAGATACTCAACCACGGTCAACTTCTTACTCTCTCCCTCGATAAAGCCTCTGTCTCTGGTTTTGAATCCACAAACGAATATCTCTTCGGCAAGATTGACATGCAGCTCAAGCTTGTTGCAGGAAACTCTGCTGGCACCGTCACTGCCTACTATTTAAAATCAGAGGGGTCTGTTTGA

Protein Analysis

101

Amino Acids

10.63

Weight (kDa)

6.53

Isoelectric Point (pI)

7.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 29 - 97 2.5e-14 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018830)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09151
pyrus_communis pycom09g07180
rosa_chinensis RchiOBHm_Chr4g0402941
rosa_laevigata RLG00000009038
rosa_samantha Rh2BG503900 Rh4AG116400 Rh4CG123600 Rh4CG123800 Rh4CG185600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AgsI TTSAA 3 cut(s) 88, 100, 191
AluBI AGCT 3 cut(s) 57, 232, 238
AluI AGCT 3 cut(s) 57, 232, 238
Alw26I GTCTC 1 cut(s) 184
ApeKI GCWGC 1 cut(s) 229
BanI GGYRCC 1 cut(s) 260
BbvI GCAGC 1 cut(s) 241
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 184
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 262
BpuEI CTTGAG 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 139
BseDI CCNNGG 1 cut(s) 139
BseXI GCAGC 1 cut(s) 241
Bsh1285I CGRYCG 1 cut(s) 121
BshNI GGYRCC 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 121
BsmAI GTCTC 1 cut(s) 184
Bsp143I GATC 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 1 cut(s) 33
Bst4CI ACNGT 3 cut(s) 119, 143, 265
Bst6I CTCTTC 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 259
BstDSI CCRYGG 1 cut(s) 139
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 1 cut(s) 33
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstNSI RCATGY 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 241
BtgI CCRYGG 1 cut(s) 139
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 1 cut(s) 267
Cac8I GCNNGC 1 cut(s) 259
CviAII CATG 2 cut(s) 108, 226
CviJI RGCY 5 cut(s) 57, 125, 173, 232, 238
CviKI_1 RGCY 5 cut(s) 57, 125, 173, 232, 238
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
DraI TTTAAA 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
FaeI CATG 2 cut(s) 111, 229
FaiI YATR 3 cut(s) 65, 109, 227
FatI CATG 2 cut(s) 107, 225
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 230
Fsp4HI GCNGC 1 cut(s) 230
GluI GCNGC 1 cut(s) 230
Hin1II CATG 2 cut(s) 111, 229
HincII GTYRAC 1 cut(s) 146
HindII GTYRAC 1 cut(s) 146
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 1 cut(s) 191
Hpy166II GTNNAC 1 cut(s) 146
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 1 cut(s) 100
Hpy8I GTNNAC 1 cut(s) 146
Hpy99I CGWCG 1 cut(s) 119
HpyCH4III ACNGT 3 cut(s) 119, 143, 265
HpyCH4V TGCA 2 cut(s) 229, 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
Hsp92II CATG 2 cut(s) 111, 229
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 1 cut(s) 33
LpnPI CCDG 4 cut(s) 63, 168, 231, 243
Lsp1109I GCAGC 1 cut(s) 241
MaeIII GTNAC 2 cut(s) 80, 265
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 1 cut(s) 202
MnlI CCTC 5 cut(s) 51, 82, 173, 184, 283
MseI TTAA 1 cut(s) 281
MwoI GCNNNNNNNGC 1 cut(s) 235
NdeII GATC 1 cut(s) 33
NlaIII CATG 2 cut(s) 111, 229
NlaIV GGNNCC 1 cut(s) 262
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 1 cut(s) 229
PfeI GAWTC 1 cut(s) 191
PkrI GCNGC 1 cut(s) 231
PspN4I GGNNCC 1 cut(s) 262
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 1 cut(s) 230
Sau3AI GATC 1 cut(s) 33
SetI ASST 3 cut(s) 59, 234, 240
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
TaaI ACNGT 3 cut(s) 119, 143, 265
TaqI TCGA 2 cut(s) 121, 165
TfiI GAWTC 1 cut(s) 191
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 52
TspRI CASTG 1 cut(s) 274
XceI RCATGY 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.