Rh4CG185600

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018830
Physical Location & Seq
Forward (+)
40040065 .. 40042546
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG185600.1

Sequence Viewer

Length: 360 bp
ATGGGGATTTCTGGTAACGTGTCTTGTTTGGTTTTGTTGGTTGGTTTAGCATTGAGCTCTTTAATAGTAGGCTCTTATGCTGGCAACTTCTATCAAGACTTTGACTTGACATGGGGTGGTCACCGTCCTAAGATATTCAAGGGAGGTCAGCTTCTTTCTTTGTCTTTGGACAAGGTTTCTGGCTCTGGATTCCAGTCCAAGAAAGAGTACCTGTTCGGGAGGATTGACATGCAACTTAAGCTTGTTGCCGGAAACTCTGCCGGCACTGTGACTGCCTACTACGTACGTATTCGGAGAAATAAAAATATATACGTAACTCGCACTGCCTATACATTTTCTTCATTATTTTCTGATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

119

Amino Acids

13.03

Weight (kDa)

9.76

Isoelectric Point (pI)

27.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 31 - 96 6.5e-14 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018830)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09151
pyrus_communis pycom09g07180
rosa_chinensis RchiOBHm_Chr4g0402941
rosa_laevigata RLG00000009038
rosa_samantha Rh2BG503900 Rh4AG116400 Rh4CG123600 Rh4CG123800 Rh4CG185600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 2 cut(s) 209, 285
AflII CTTAAG 1 cut(s) 236
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 1 cut(s) 139
AluBI AGCT 3 cut(s) 57, 151, 241
AluI AGCT 3 cut(s) 57, 151, 241
Alw21I GWGCWC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 113
BanII GRGCYC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 59
BfrI CTTAAG 1 cut(s) 236
BsaAI YACGTR 3 cut(s) 283, 287, 313
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 193
BseNI ACTGG 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 59
BsiSI CCGG 2 cut(s) 249, 261
BsiWI CGTACG 1 cut(s) 283
Bsp1286I GDGCHC 1 cut(s) 59
BspTI CTTAAG 1 cut(s) 236
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 1 cut(s) 193
BssAI RCCGGY 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 125, 268
BstAFI CTTAAG 1 cut(s) 236
BstBAI YACGTR 3 cut(s) 283, 287, 313
BstC8I GCNNGC 2 cut(s) 82, 262
BstDEI CTNAG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 119
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstNSI RCATGY 1 cut(s) 232
BstPI GGTNACC 1 cut(s) 119
BstSNI TACGTA 3 cut(s) 283, 287, 313
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 2 cut(s) 264, 321
Cac8I GCNNGC 2 cut(s) 82, 262
Cfr10I RCCGGY 1 cut(s) 260
Csp6I GTAC 2 cut(s) 208, 284
CviAII CATG 2 cut(s) 111, 229
CviJI RGCY 5 cut(s) 57, 72, 151, 183, 241
CviKI_1 RGCY 5 cut(s) 57, 72, 151, 183, 241
CviQI GTAC 2 cut(s) 208, 284
DdeI CTNAG 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 57
Eco105I TACGTA 3 cut(s) 283, 287, 313
Eco24I GRGCYC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 57
Eco91I GGTNACC 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 57
EcoO65I GGTNACC 1 cut(s) 119
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 2 cut(s) 114, 232
FaiI YATR 8 cut(s) 78, 112, 230, 308, 310, 330, 356, 358
FatI CATG 2 cut(s) 110, 228
FriOI GRGCYC 1 cut(s) 59
HapII CCGG 2 cut(s) 249, 261
Hin1II CATG 2 cut(s) 114, 232
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 1 cut(s) 189
HpaII CCGG 2 cut(s) 249, 261
HphI GGTGA 1 cut(s) 113
Hpy188I TCNGA 2 cut(s) 294, 352
Hpy188III TCNNGA 3 cut(s) 95, 186, 217
HpyCH4III ACNGT 2 cut(s) 125, 268
HpyCH4IV ACGT 4 cut(s) 18, 282, 286, 312
HpyCH4V TGCA 1 cut(s) 232
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 4 cut(s) 18, 282, 286, 312
Hsp92II CATG 2 cut(s) 114, 232
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
LpnPI CCDG 7 cut(s) 66, 165, 171, 206, 224, 262, 274
MaeII ACGT 4 cut(s) 18, 282, 286, 312
MaeIII GTNAC 4 cut(s) 14, 119, 268, 313
MboII GAAGA 1 cut(s) 330
MhlI GDGCHC 1 cut(s) 59
MnlI CCTC 2 cut(s) 137, 213
MroNI GCCGGC 1 cut(s) 260
MseI TTAA 2 cut(s) 62, 237
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 2 cut(s) 249, 261
MwoI GCNNNNNNNGC 1 cut(s) 238
NaeI GCCGGC 1 cut(s) 262
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 2 cut(s) 114, 232
NmuCI GTSAC 2 cut(s) 119, 268
NspI RCATGY 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 262
PfeI GAWTC 1 cut(s) 189
Pfl23II CGTACG 1 cut(s) 283
Ppu21I YACGTR 3 cut(s) 283, 287, 313
Psp124BI GAGCTC 1 cut(s) 59
PspEI GGTNACC 1 cut(s) 119
PspLI CGTACG 1 cut(s) 283
RsaI GTAC 2 cut(s) 209, 285
RsaNI GTAC 2 cut(s) 208, 284
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 2 cut(s) 62, 237
SduI GDGCHC 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SnaBI TACGTA 3 cut(s) 283, 287, 313
SstI GAGCTC 1 cut(s) 59
TaaI ACNGT 2 cut(s) 125, 268
TaiI ACGT 4 cut(s) 21, 285, 289, 315
TfiI GAWTC 1 cut(s) 189
Tru1I TTAA 2 cut(s) 62, 237
Tru9I TTAA 2 cut(s) 62, 237
TscAI CASTG 2 cut(s) 271, 328
TseFI GTSAC 2 cut(s) 119, 268
Tsp45I GTSAC 2 cut(s) 119, 268
TspDTI ATGAA 1 cut(s) 330
TspRI CASTG 2 cut(s) 271, 328
Vha464I CTTAAG 1 cut(s) 236
XceI RCATGY 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.