pycom09g07180

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0018830
Physical Location & Seq
Reverse (-)
5407027 .. 5407804
778 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g07180.1

Sequence Viewer

Length: 639 bp
ATGAATATGGTGTTTCTTCTTTCTCTGTTCACAACTTCTCTAATGGCCATGACTGCCTCAGCTGGTAATTTCTTTCAAGACTTTGACATTACATTTGGCGACGGACGTGCCAAGATACTCAACGGAGGACAGCTTCTCACACTCAACCTTGATAAGGCCTCTGGGTCTGGCTTCAAGTCCAAGAATGAGTACTTATTTGGAAGGATTGACATGCAGATCAAGCTCGTCTCCGGCAACTCAGCTGGAACCGTTACTGCCTACTATGTAAGTACTAGTACTGCCCTCATAATTATACCCTTTAATTCTAAATATATATTCCATAATTGCATTGCTAATTTGTTAAACAAATTGCATATTTTCAGTTATCTTCTGAAGGTCCAACCCATGACGAGATTGATTTCGAGTTTTTGGGCAACTCAACTGGTGAACCCTACACTCTCCATACCAATGTCTTCAGCCAAGGAAAAGGGAACAGAGAACAACAATTCCAGCTTTGGTTTGACCCCACAAAAACCTTCCACACATACTCCATTGTTTGGAACTCGCAGCGCATTATATTCTTGGTGGATAACATTCCAATCAGAGTGTTCAACAACTTGGAATCAGCTGGAGTCCCATTCCCTAAAAACCAGCCCATGA

Protein Analysis

213

Amino Acids

23.48

Weight (kDa)

9.39

Isoelectric Point (pI)

26.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 26 - 91 2e-13 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018830)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09151
pyrus_communis pycom09g07180
rosa_chinensis RchiOBHm_Chr4g0402941
rosa_laevigata RLG00000009038
rosa_samantha Rh2BG503900 Rh4AG116400 Rh4CG123600 Rh4CG123800 Rh4CG185600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 536
AcoI YGGCCR 1 cut(s) 45
AcuI CTGAAG 2 cut(s) 392, 438
AfaI GTAC 3 cut(s) 191, 271, 277
AfiI CCNNNNNNNGG 2 cut(s) 154, 536
AgsI TTSAA 3 cut(s) 77, 175, 591
AhlI ACTAGT 1 cut(s) 272
AjiI CACGTC 1 cut(s) 107
AloI GAACNNNNNNTCC 2 cut(s) 470, 502
AluBI AGCT 6 cut(s) 62, 133, 223, 242, 492, 607
AluI AGCT 6 cut(s) 62, 133, 223, 242, 492, 607
Alw26I GTCTC 1 cut(s) 232
AoxI GGCC 2 cut(s) 45, 156
ApeKI GCWGC 1 cut(s) 546
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspLEI GCGC 1 cut(s) 551
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 436
AvaII GGWCC 1 cut(s) 376
BalI TGGCCA 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 444
BbvCI CCTCAGC 1 cut(s) 58
BbvI GCAGC 1 cut(s) 558
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BcoDI GTCTC 1 cut(s) 232
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 1 cut(s) 273
BisI GCNGC 1 cut(s) 547
BlsI GCNGC 1 cut(s) 548
BmcAI AGTACT 3 cut(s) 191, 271, 277
Bme18I GGWCC 1 cut(s) 376
BmgBI CACGTC 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 247
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 58
BsaJI CCNNGG 1 cut(s) 459
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 536
Bse1I ACTGG 1 cut(s) 426
Bse3DI GCAATG 1 cut(s) 327
BseDI CCNNGG 1 cut(s) 459
BseLI CCNNNNNNNGG 2 cut(s) 154, 536
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 72, 252
BseNI ACTGG 1 cut(s) 426
BseXI GCAGC 1 cut(s) 558
BshFI GGCC 2 cut(s) 47, 158
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 2 cut(s) 154, 536
BsmAI GTCTC 1 cut(s) 232
BsmBI CGTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 598
BsnI GGCC 2 cut(s) 47, 158
Bsp143I GATC 1 cut(s) 216
BspANI GGCC 2 cut(s) 47, 158
BspCNI CTCAG 2 cut(s) 71, 251
BspLI GGNNCC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 327
BsrI ACTGG 1 cut(s) 426
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 250
BstDEI CTNAG 2 cut(s) 58, 238
BstENI CCTNNNNNAGG 1 cut(s) 152
BstHHI GCGC 1 cut(s) 551
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 1 cut(s) 216
BstMWI GCNNNNNNNGC 2 cut(s) 53, 220
BstNSI RCATGY 1 cut(s) 214
BstV1I GCAGC 1 cut(s) 558
BstV2I GAAGAC 1 cut(s) 444
BsuRI GGCC 2 cut(s) 47, 158
BtrI CACGTC 1 cut(s) 107
CfoI GCGC 1 cut(s) 551
Cfr13I GGNCC 1 cut(s) 376
Csp6I GTAC 3 cut(s) 190, 270, 276
CviAII CATG 4 cut(s) 49, 211, 385, 636
CviQI GTAC 3 cut(s) 190, 270, 276
DdeI CTNAG 2 cut(s) 58, 238
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
EaeI YGGCCR 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 459
Eco147I AGGCCT 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 2 cut(s) 392, 438
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 4 cut(s) 52, 214, 388, 639
FaqI GGGAC 1 cut(s) 598
FatI CATG 4 cut(s) 48, 210, 384, 635
Fnu4HI GCNGC 1 cut(s) 547
Fsp4HI GCNGC 1 cut(s) 547
FspBI CTAG 1 cut(s) 273
GlaI GCGC 1 cut(s) 550
GluI GCNGC 1 cut(s) 547
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 2 cut(s) 47, 158
HapII CCGG 1 cut(s) 231
HhaI GCGC 1 cut(s) 551
Hin1II CATG 4 cut(s) 52, 214, 388, 639
Hin6I GCGC 1 cut(s) 549
HinP1I GCGC 1 cut(s) 549
HinfI GANTC 2 cut(s) 601, 611
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 436
Hpy166II GTNNAC 2 cut(s) 30, 427
Hpy188I TCNGA 2 cut(s) 372, 583
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 2 cut(s) 30, 427
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 195, 367, 525
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 3 cut(s) 214, 327, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 220
HpyF3I CTNAG 2 cut(s) 58, 238
HpySE526I ACGT 1 cut(s) 106
Hsp92II CATG 4 cut(s) 52, 214, 388, 639
HspAI GCGC 1 cut(s) 549
Kzo9I GATC 1 cut(s) 216
LpnPI CCDG 8 cut(s) 48, 147, 153, 228, 244, 407, 502, 593
Lsp1109I GCAGC 1 cut(s) 558
MaeI CTAG 1 cut(s) 273
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 1 cut(s) 250
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MboII GAAGA 3 cut(s) 8, 359, 444
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 7 cut(s) 67, 288, 301, 322, 334, 347, 484
MluNI TGGCCA 1 cut(s) 47
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 4 cut(s) 67, 119, 169, 293
Mox20I TGGCCA 1 cut(s) 47
MscI TGGCCA 1 cut(s) 47
MseI TTAA 2 cut(s) 300, 341
MslI CAYNNNNRTG 1 cut(s) 446
Msp20I TGGCCA 1 cut(s) 47
MspA1I CMGCKG 3 cut(s) 62, 242, 607
MspI CCGG 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 53, 220
NdeII GATC 1 cut(s) 216
NlaIII CATG 4 cut(s) 52, 214, 388, 639
NlaIV GGNNCC 1 cut(s) 247
NspI RCATGY 1 cut(s) 214
PceI AGGCCT 1 cut(s) 158
PfeI GAWTC 1 cut(s) 601
PflMI CCANNNNNTGG 1 cut(s) 536
PkrI GCNGC 1 cut(s) 548
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 376
PvuII CAGCTG 3 cut(s) 62, 242, 607
RsaI GTAC 3 cut(s) 191, 271, 277
RsaNI GTAC 3 cut(s) 190, 270, 276
RseI CAYNNNNRTG 1 cut(s) 446
SaqAI TTAA 2 cut(s) 300, 341
SatI GCNGC 1 cut(s) 547
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 1 cut(s) 376
ScaI AGTACT 3 cut(s) 191, 271, 277
SchI GAGTC 1 cut(s) 620
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 446
SpeI ACTAGT 1 cut(s) 272
Sse9I AATT 7 cut(s) 67, 288, 301, 322, 334, 347, 484
SseBI AGGCCT 1 cut(s) 158
SspMI CTAG 1 cut(s) 273
StuI AGGCCT 1 cut(s) 158
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 1 cut(s) 109
TaqI TCGA 1 cut(s) 401
TasI AATT 7 cut(s) 67, 288, 301, 322, 334, 347, 484
TatI WGTACW 3 cut(s) 189, 269, 275
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 2 cut(s) 300, 341
Tru9I TTAA 2 cut(s) 300, 341
TseI GCWGC 1 cut(s) 546
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 117, 138
Van91I CCANNNNNTGG 1 cut(s) 536
VpaK11BI GGWCC 1 cut(s) 376
XagI CCTNNNNNAGG 1 cut(s) 152
XceI RCATGY 1 cut(s) 214
XspI CTAG 1 cut(s) 273
ZrmI AGTACT 3 cut(s) 191, 271, 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.