Rh4AG116400

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0018830
Physical Location & Seq
Forward (+)
25141949 .. 25142758
810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG116400.1

Sequence Viewer

Length: 471 bp
ATGGCTTCTTTCAAATCTGCTCTTTCACTGATGCTGCTTGCTGTACCTCTTCTTGTTATGGTTGCCTCTGCGGGTAACTTCAACCAGGACTTTCAAATTACATGGGGAGATGGCCGAGCCAAGATACTCAACAATGGCCAACTTCTTACTCTCACGCTCGACAAAGCCTCTGGCTCTGGTTTCGAATCCAAAAATGAATATCTCTTCGGCAAAATCGATATGCAGCTCAAGCTTGTTGCTGGGAACTCTGCTGGAACTGTCACCGCCTACTATTTGCGCTCAGAAGGATCATTATGGGATGAAATAGACTTTGAGTTCTTGGGAAACCTGAGTGGTGACCCTTACATTCTTCACACCAATGTGTTCACCCAAGGCAAAGGAAACAGAGAGCAACAATTCTATCTCTGGTTCGACCCAACTGCTGATTTCCACACCTATTCCATTCTCTGGAATCCACAGCGCATTGTGTAA

Protein Analysis

156

Amino Acids

17.44

Weight (kDa)

5.01

Isoelectric Point (pI)

18.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 27 - 156 3.2e-45 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018830)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09151
pyrus_communis pycom09g07180
rosa_chinensis RchiOBHm_Chr4g0402941
rosa_laevigata RLG00000009038
rosa_samantha Rh2BG503900 Rh4AG116400 Rh4CG123600 Rh4CG123800 Rh4CG185600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 447
AciI CCGC 2 cut(s) 71, 264
AclWI GGATC 1 cut(s) 295
AcoI YGGCCR 2 cut(s) 112, 136
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 447
AgsI TTSAA 3 cut(s) 13, 82, 95
AjnI CCWGG 1 cut(s) 84
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 2 cut(s) 226, 232
AluI AGCT 2 cut(s) 226, 232
AlwI GGATC 1 cut(s) 295
AoxI GGCC 2 cut(s) 112, 136
ApeKI GCWGC 2 cut(s) 34, 223
AspLEI GCGC 2 cut(s) 279, 462
AsuHPI GGTGA 3 cut(s) 253, 347, 358
AsuII TTCGAA 1 cut(s) 183
BalI TGGCCA 1 cut(s) 138
BbvI GCAGC 2 cut(s) 21, 235
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 1 cut(s) 86
BisI GCNGC 2 cut(s) 35, 224
BlsI GCNGC 2 cut(s) 36, 225
Bme1390I CCNGG 1 cut(s) 86
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 1 cut(s) 21
Bpu14I TTCGAA 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 212
Bsa29I ATCGAT 1 cut(s) 216
BsaJI CCNNGG 1 cut(s) 370
Bsc4I CCNNNNNNNGG 1 cut(s) 447
BseBI CCWGG 1 cut(s) 86
BseCI ATCGAT 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 1 cut(s) 447
BseMII CTCAG 2 cut(s) 294, 320
BseXI GCAGC 2 cut(s) 21, 235
BseYI CCCAGC 1 cut(s) 239
BshFI GGCC 2 cut(s) 114, 138
BshVI ATCGAT 1 cut(s) 216
BslI CCNNNNNNNGG 1 cut(s) 447
BsnI GGCC 2 cut(s) 114, 138
Bsp119I TTCGAA 1 cut(s) 183
Bsp143I GATC 1 cut(s) 287
BspACI CCGC 2 cut(s) 71, 264
BspANI GGCC 2 cut(s) 114, 138
BspCNI CTCAG 2 cut(s) 293, 321
BspDI ATCGAT 1 cut(s) 216
BspPI GGATC 1 cut(s) 295
BspT104I TTCGAA 1 cut(s) 183
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 1 cut(s) 287
BssT1I CCWWGG 1 cut(s) 370
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 2 cut(s) 54, 209
BstBI TTCGAA 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 2 cut(s) 280, 329
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 1 cut(s) 304
BstHHI GCGC 2 cut(s) 279, 462
BstKTI GATC 1 cut(s) 290
BstMBI GATC 1 cut(s) 287
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstNI CCWGG 1 cut(s) 86
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 21, 235
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 2 cut(s) 114, 138
BsuTUI ATCGAT 1 cut(s) 216
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 1 cut(s) 39
CfoI GCGC 2 cut(s) 279, 462
ClaI ATCGAT 1 cut(s) 216
Csp6I GTAC 1 cut(s) 44
CviAII CATG 1 cut(s) 102
CviJI RGCY 8 cut(s) 5, 114, 119, 138, 167, 174, 226, 232
CviKI_1 RGCY 8 cut(s) 5, 114, 119, 138, 167, 174, 226, 232
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 2 cut(s) 280, 329
DpnI GATC 1 cut(s) 289
DpnII GATC 1 cut(s) 287
EaeI YGGCCR 2 cut(s) 112, 136
Eam1104I CTCTTC 2 cut(s) 54, 209
EarI CTCTTC 2 cut(s) 54, 209
Eco130I CCWWGG 1 cut(s) 370
Eco91I GGTNACC 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 1 cut(s) 105
FaiI YATR 4 cut(s) 59, 103, 221, 295
FatI CATG 1 cut(s) 101
FauI CCCGC 1 cut(s) 64
Fnu4HI GCNGC 2 cut(s) 35, 224
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 35, 224
GlaI GCGC 2 cut(s) 278, 461
GluI GCNGC 2 cut(s) 35, 224
GsaI CCCAGC 1 cut(s) 243
HaeIII GGCC 2 cut(s) 114, 138
HhaI GCGC 2 cut(s) 279, 462
Hin1II CATG 1 cut(s) 105
Hin6I GCGC 2 cut(s) 277, 460
HinP1I GCGC 2 cut(s) 277, 460
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 2 cut(s) 185, 451
HphI GGTGA 3 cut(s) 253, 347, 358
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 1 cut(s) 283
Hpy188III TCNNGA 1 cut(s) 448
Hpy8I GTNNAC 1 cut(s) 366
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
HpyF3I CTNAG 2 cut(s) 280, 329
Hsp92II CATG 1 cut(s) 105
HspAI GCGC 2 cut(s) 277, 460
Kzo9I GATC 1 cut(s) 287
LpnPI CCDG 9 cut(s) 71, 98, 156, 162, 225, 237, 341, 391, 433
Lsp1109I GCAGC 2 cut(s) 21, 235
LweI GCATC 1 cut(s) 21
MaeIII GTNAC 3 cut(s) 74, 259, 335
MalI GATC 1 cut(s) 289
MboI GATC 1 cut(s) 287
MboII GAAGA 3 cut(s) 41, 196, 341
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 2 cut(s) 96, 395
MluNI TGGCCA 1 cut(s) 138
MnlI CCTC 3 cut(s) 57, 76, 178
Mox20I TGGCCA 1 cut(s) 138
MscI TGGCCA 1 cut(s) 138
MslI CAYNNNNRTG 2 cut(s) 357, 359
Msp20I TGGCCA 1 cut(s) 138
MspR9I CCNGG 1 cut(s) 86
MvaI CCWGG 1 cut(s) 86
MwoI GCNNNNNNNGC 1 cut(s) 229
NdeII GATC 1 cut(s) 287
NlaIII CATG 1 cut(s) 105
NmeAIII GCCGAG 1 cut(s) 140
NmuCI GTSAC 2 cut(s) 259, 335
NspV TTCGAA 1 cut(s) 183
OliI CACNNNNGTG 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PfeI GAWTC 2 cut(s) 185, 451
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 2 cut(s) 36, 225
Psp6I CCWGG 1 cut(s) 84
PspEI GGTNACC 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 1 cut(s) 84
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
RseI CAYNNNNRTG 2 cut(s) 357, 359
SatI GCNGC 2 cut(s) 35, 224
Sau3AI GATC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 86
SetI ASST 5 cut(s) 49, 228, 234, 330, 437
SfaNI GCATC 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 183
SmiMI CAYNNNNRTG 2 cut(s) 357, 359
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 96, 395
SsiI CCGC 2 cut(s) 71, 264
StyD4I CCNGG 1 cut(s) 84
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 259
TaqI TCGA 4 cut(s) 159, 183, 216, 411
TasI AATT 2 cut(s) 96, 395
TfiI GAWTC 2 cut(s) 185, 451
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 2 cut(s) 259, 335
TseI GCWGC 2 cut(s) 34, 223
Tsp45I GTSAC 2 cut(s) 259, 335
TspDTI ATGAA 2 cut(s) 210, 315
TspRI CASTG 1 cut(s) 33
Van91I CCANNNNNTGG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.