Rh4CG123800

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018830
Physical Location & Seq
Reverse (-)
23919027 .. 23919931
905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG123800.1

Sequence Viewer

Length: 333 bp
ATGGCTTCATCCAGTTCACTAATGTTGCTGACTATTGCTCTTCTTATTAGCTCTTTCATGGTTGCCTCTGCCGGTAACTTCAACCAAGACTTTAAGATTACATGGGGGGATGGTCGAGCCAATATACTCAACGACGGTCAACTTCTTACTCTTTCGCTCGACAAAGCCTCTGGCTCTGGTTTTGAATCCCAAAACGAATATCTATTCGGCAAGATTGACATGCAGCTCAAACTTGTTGCCGGAAACTCTGCTGGAACTGTCACTGCCTATTATGTATGTCTTCATTTTGGAATTTATCTATTTTATTGCTTCATATTAATTCTCTATTTCTAA

Protein Analysis

110

Amino Acids

12.06

Weight (kDa)

4.94

Isoelectric Point (pI)

21.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 27 - 93 2.6e-14 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018830)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09151
pyrus_communis pycom09g07180
rosa_chinensis RchiOBHm_Chr4g0402941
rosa_laevigata RLG00000009038
rosa_samantha Rh2BG503900 Rh4AG116400 Rh4CG123600 Rh4CG123800 Rh4CG185600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 291
AgsI TTSAA 2 cut(s) 82, 185
AluBI AGCT 2 cut(s) 51, 226
AluI AGCT 2 cut(s) 51, 226
ApeKI GCWGC 1 cut(s) 223
ApoI RAATTY 1 cut(s) 291
AseI ATTAAT 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 272
BbvI GCAGC 1 cut(s) 235
BccI CCATC 1 cut(s) 104
BisI GCNGC 1 cut(s) 224
BlsI GCNGC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 272
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 12
BseGI GGATG 2 cut(s) 8, 115
BseNI ACTGG 1 cut(s) 12
BseXI GCAGC 1 cut(s) 235
BsiSI CCGG 2 cut(s) 72, 240
BspQI GCTCTTC 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 12
BssAI RCCGGY 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 137, 259
Bst6I CTCTTC 1 cut(s) 45
BstF5I GGATG 2 cut(s) 8, 115
BstNSI RCATGY 1 cut(s) 223
BstV1I GCAGC 1 cut(s) 235
BstV2I GAAGAC 1 cut(s) 272
BtsCI GGATG 2 cut(s) 8, 115
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 1 cut(s) 261
Cfr10I RCCGGY 1 cut(s) 71
CviAII CATG 3 cut(s) 58, 102, 220
CviJI RGCY 6 cut(s) 5, 51, 119, 167, 174, 226
CviKI_1 RGCY 6 cut(s) 5, 51, 119, 167, 174, 226
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
FaeI CATG 3 cut(s) 61, 105, 223
FaiI YATR 7 cut(s) 59, 103, 125, 221, 273, 277, 314
FatI CATG 3 cut(s) 57, 101, 219
Fnu4HI GCNGC 1 cut(s) 224
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 1 cut(s) 224
GluI GCNGC 1 cut(s) 224
HapII CCGG 2 cut(s) 72, 240
Hin1II CATG 3 cut(s) 61, 105, 223
HincII GTYRAC 1 cut(s) 140
HindII GTYRAC 1 cut(s) 140
HinfI GANTC 1 cut(s) 185
HpaII CCGG 2 cut(s) 72, 240
Hpy166II GTNNAC 2 cut(s) 17, 140
Hpy8I GTNNAC 2 cut(s) 17, 140
Hpy99I CGWCG 1 cut(s) 137
HpyCH4III ACNGT 2 cut(s) 137, 259
HpyCH4V TGCA 1 cut(s) 223
Hsp92II CATG 3 cut(s) 61, 105, 223
LguI GCTCTTC 1 cut(s) 45
LpnPI CCDG 6 cut(s) 25, 85, 156, 162, 237, 253
Lsp1109I GCAGC 1 cut(s) 235
MaeIII GTNAC 2 cut(s) 74, 259
MboII GAAGA 2 cut(s) 32, 272
MluCI AATT 2 cut(s) 291, 318
MnlI CCTC 2 cut(s) 76, 178
MseI TTAA 2 cut(s) 93, 317
MspI CCGG 2 cut(s) 72, 240
NlaIII CATG 3 cut(s) 61, 105, 223
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 185
PkrI GCNGC 1 cut(s) 225
PshBI ATTAAT 1 cut(s) 317
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 2 cut(s) 93, 317
SatI GCNGC 1 cut(s) 224
SetI ASST 2 cut(s) 53, 228
Sse9I AATT 2 cut(s) 291, 318
TaaI ACNGT 2 cut(s) 137, 259
TaqI TCGA 2 cut(s) 115, 159
TasI AATT 2 cut(s) 291, 318
TfiI GAWTC 1 cut(s) 185
Tru1I TTAA 2 cut(s) 93, 317
Tru9I TTAA 2 cut(s) 93, 317
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 3 cut(s) 46, 272, 301
TspRI CASTG 1 cut(s) 268
VspI ATTAAT 1 cut(s) 317
XapI RAATTY 1 cut(s) 291
XceI RCATGY 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.